Planarian EST Database


Dr_sW_007_A23

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_007_A23
         (834 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q01241|NF70_LOLPE  70 kDa neurofilament protein (NF70)          65   2e-10
sp|Q01240|NF60_LOLPE  60 kDa neurofilament protein (NF60)          65   2e-10
sp|P23731|IFEB_ASCSU  Intermediate filament protein B (IF-B)       50   6e-06
sp|P90900|IFA4_CAEEL  Intermediate filament protein ifa-4 (I...    47   5e-05
sp|P23730|IFEA_ASCSU  Intermediate filament protein A (IF-A)       45   2e-04
sp|O02365|IFA2_CAEEL  Intermediate filament protein ifa-2 (I...    45   2e-04
sp|Q19289|IFB1_CAEEL  Intermediate filament protein ifb-1 (I...    45   3e-04
sp|P90901|IFA1_CAEEL  Intermediate filament protein ifa-1 (I...    45   3e-04
sp|Q21065|IFA3_CAEEL  Intermediate filament protein ifa-3 (I...    44   4e-04
sp|P31732|OV71_ONCVO  Muscle cell intermediate filament prot...    44   4e-04
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
          Length = 615

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 39/93 (41%), Positives = 51/93 (54%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +KS +  +R+R+ADLE +NA+ E     +    EEK R  ELE   ++E++T        
Sbjct: 357 LKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMES 416

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESRV 281
                      KL LELEIAAY KLLE EESRV
Sbjct: 417 ILKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
          Length = 511

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 39/93 (41%), Positives = 51/93 (54%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +KS +  +R+R+ADLE +NA+ E     +    EEK R  ELE   ++E++T        
Sbjct: 357 LKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMES 416

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESRV 281
                      KL LELEIAAY KLLE EESRV
Sbjct: 417 ILKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
          Length = 589

 Score = 50.4 bits (119), Expect = 6e-06
 Identities = 30/67 (44%), Positives = 42/67 (62%), Gaps = 1/67 (1%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           S +  F+R+ KGN++I+E D NG FITLEN  + K E +    LKR +D + + IV+TI 
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIP 523

Query: 798 NKTTLNA 818
             T L A
Sbjct: 524 PNTVLKA 530

 Score = 38.1 bits (87), Expect = 0.030
 Identities = 27/93 (29%), Positives = 38/93 (40%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           ++  I  +R ++ DLE +NA  E  +  +     + QR  E  L      L         
Sbjct: 341 MRDNIGDLRGKLGDLEAKNALLEKEVQNLNYQLNDDQRQYEAALNDRDATLRRMREECQT 400

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESRV 281
                      K  L+ EIA Y K+LE EESRV
Sbjct: 401 LVAELQALLDTKQMLDAEIAIYRKMLEGEESRV 433
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
           A4) (IF-A4) (Cel IF A4)
          Length = 575

 Score = 47.4 bits (111), Expect = 5e-05
 Identities = 26/61 (42%), Positives = 38/61 (62%)
 Frame = +3

Query: 636 FRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADIIVFTIQNKTTLN 815
           + R+ KGNI+I E + +G +I LENI ++ E I    L+R I G+ + IVFT   + TL 
Sbjct: 460 YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLR 518

Query: 816 A 818
           A
Sbjct: 519 A 519
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
          Length = 497

 Score = 45.4 bits (106), Expect = 2e-04
 Identities = 24/55 (43%), Positives = 35/55 (63%), Gaps = 1/55 (1%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADII 782
           + R  F+R+ KGN+SI ET  +G ++ LEN  + K E I    LKR IDG+ +I+
Sbjct: 374 ASRTSFQRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKREIV 428
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
           A2) (IF-A2) (Cel IF A2)
          Length = 581

 Score = 45.1 bits (105), Expect = 2e-04
 Identities = 27/66 (40%), Positives = 39/66 (59%), Gaps = 1/66 (1%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           S R  ++R+ KGN++I ET   G F+ LEN  + K E +    LKR IDG+ + IVFT  
Sbjct: 456 SSRTSYQRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFP 514

Query: 798 NKTTLN 815
           +   L+
Sbjct: 515 SDYILH 520

 Score = 30.8 bits (68), Expect = 4.8
 Identities = 20/87 (22%), Positives = 36/87 (41%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +++Q   +R +++DLE +N   E  +  +    E+ QRS E  L     Q+         
Sbjct: 332 LRNQTSELRQKLSDLESRNLLLEKQIEDLNYQLEDDQRSYEAALNDKDAQIRKLREECQA 391

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLE 263
                      K  L+ E+  Y ++LE
Sbjct: 392 LMVELQMLLDTKQTLDGELKVYRQMLE 418
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
           B1) (IF-B1) (Cel IF B1)
          Length = 589

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 24/67 (35%), Positives = 40/67 (59%), Gaps = 1/67 (1%)
 Frame = +3

Query: 585 NELSSQEAGAYQSKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNI 761
           N  S++      S +  F+R+ KGN++I+E D NG FI LEN  + K E +    ++R +
Sbjct: 453 NTDSTRSVRGEVSTKTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKL 512

Query: 762 DGQADII 782
           DG+ +I+
Sbjct: 513 DGRREIV 519
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
           A1) (IF-A1) (Cel IF A1)
          Length = 575

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 27/92 (29%), Positives = 43/92 (46%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +++Q+  +R ++ADLE +N+  E  +  +    E+ QRS E  L     Q+         
Sbjct: 330 LRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQA 389

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESR 278
                      K  L+ EIA Y K+LE EE+R
Sbjct: 390 LMVELQMLLDTKQTLDAEIAIYRKMLEGEENR 421

 Score = 43.1 bits (100), Expect = 0.001
 Identities = 27/67 (40%), Positives = 38/67 (56%), Gaps = 1/67 (1%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           + R  F+R+ KGN+SI E   +G FI L+N  + K E I    LKR IDG+ +  V+T+ 
Sbjct: 454 ASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRE-NVYTLP 512

Query: 798 NKTTLNA 818
               L A
Sbjct: 513 RDFVLRA 519
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
           A3) (IF-A3) (Cel IF A3)
          Length = 581

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 27/66 (40%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           S R  ++R+ KGN+SI E    G F+ LEN  + K E +    LKR IDG+ + IVFT  
Sbjct: 456 SSRTSYQRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDGKRE-IVFTFP 514

Query: 798 NKTTLN 815
           +   L+
Sbjct: 515 SDYILH 520
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
          Length = 432

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 24/55 (43%), Positives = 34/55 (61%), Gaps = 1/55 (1%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADII 782
           + R  F+R+ KGN+SI +   +G FI LEN  + K E I    LKR IDG+ +I+
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKREIV 363

 Score = 42.4 bits (98), Expect = 0.002
 Identities = 27/92 (29%), Positives = 42/92 (45%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           ++ Q+  +R ++ADLE +N+  E  +  +    E+ QRS E  L     Q+         
Sbjct: 185 LRVQLSDLRGKLADLEGRNSLLEKQMQELNYQLEDDQRSYEAALNDRDAQIRKMREECQA 244

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESR 278
                      K  L+ EIA Y K+LE EE+R
Sbjct: 245 LMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 276
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,273,375
Number of Sequences: 369166
Number of extensions: 715041
Number of successful extensions: 2407
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2290
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2394
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8100769320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)