Planaria EST Database

DrC_00174

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00174
         (672 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9D8V7|SPC21_MOUSE  Microsomal signal peptidase 21 kDa su...   298   9e-81
sp|Q9R0P6|SPC18_MOUSE  Microsomal signal peptidase 18 kDa su...   298   9e-81
sp|Q9WTR7|SPC21_RAT  Microsomal signal peptidase 21 kDa subu...   297   2e-80
sp|P13679|SPC21_CANFA  Microsomal signal peptidase 21 kDa su...   296   3e-80
sp|P67812|SPC18_HUMAN  Microsomal signal peptidase 18 kDa su...   296   3e-80
sp|Q9BY50|SPC21_HUMAN  Microsomal signal peptidase 21 kDa su...   295   6e-80
sp|P42667|SPC18_RAT  Microsomal signal peptidase 18 kDa subu...   295   7e-80
sp|P15367|SEC11_YEAST  Signal sequence processing protein SEC11   149   5e-36
sp|P54506|LEPW_BACSU  Signal peptidase I W (SPase I) (Leader...    52   2e-06
sp|Q58920|YF25_METJA  Hypothetical protein MJ1525                  32   2.0  

>sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
           (SPC21) (SEC11-like 3)
          Length = 192

 Score =  298 bits (763), Expect = 9e-81
 Identities = 140/178 (78%), Positives = 163/178 (91%)
 Frame = +1

Query: 64  SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 243
           S+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF 
Sbjct: 15  SLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFH 74

Query: 244 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 423
           RGDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDD
Sbjct: 75  RGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDD 134

Query: 424 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
           RGLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L  +G +VL  RES
Sbjct: 135 RGLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192

>sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
           (SPC18) (Endopeptidase SP18) (SEC11-like 1) (Sid 2895)
          Length = 179

 Score =  298 bits (763), Expect = 9e-81
 Identities = 140/179 (78%), Positives = 164/179 (91%)
 Frame = +1

Query: 58  LFSMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPA 237
           + S+   DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPA
Sbjct: 1   MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPA 60

Query: 238 FFRGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSV 417
           F RGDLLFLTN  ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+V
Sbjct: 61  FHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAV 120

Query: 418 DDRGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 594
           DDRGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKFKYA+L  LGLFVL HRE
Sbjct: 121 DDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179

>sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
           (SPC21) (SEC11-like 3)
          Length = 192

 Score =  297 bits (760), Expect = 2e-80
 Identities = 139/178 (78%), Positives = 163/178 (91%)
 Frame = +1

Query: 64  SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 243
           S+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF 
Sbjct: 15  SLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFH 74

Query: 244 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 423
           RGDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDD
Sbjct: 75  RGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDD 134

Query: 424 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
           RGLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA++  +G +VL  RES
Sbjct: 135 RGLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALVAVMGAYVLLKRES 192

>sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
           (SPC21) (SEC11-like 3)
          Length = 192

 Score =  296 bits (759), Expect = 3e-80
 Identities = 139/177 (78%), Positives = 162/177 (91%)
 Frame = +1

Query: 67  MSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFR 246
           + IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF R
Sbjct: 16  LDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHR 75

Query: 247 GDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDR 426
           GDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDR
Sbjct: 76  GDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDR 135

Query: 427 GLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
           GLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L  +G +VL  RES
Sbjct: 136 GLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192

>sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
           (SPC18) (Endopeptidase SP18) (SEC11-like 1)
 sp|P67811|SPC18_CANFA Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
           (SPC18) (Endopeptidase SP18) (SEC11-like 1)
 sp|P67810|SPC18_BOVIN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
           (SPC18) (Endopeptidase SP18) (SEC11-like 1)
 sp|Q5R9C7|SPC18_PONPY Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
           (SPC18) (Endopeptidase SP18) (SEC11-like 1)
          Length = 179

 Score =  296 bits (758), Expect = 3e-80
 Identities = 139/179 (77%), Positives = 164/179 (91%)
 Frame = +1

Query: 58  LFSMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPA 237
           + S+   DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPA
Sbjct: 1   MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPA 60

Query: 238 FFRGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSV 417
           F RGDLLFLTN  ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK++G IKFLTKGDNN+V
Sbjct: 61  FHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAV 120

Query: 418 DDRGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 594
           DDRGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKFKYA+L  LGLFVL HRE
Sbjct: 121 DDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179

>sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
           (SPC21) (SEC11-like 3)
 sp|Q5RC30|SPC21_PONPY Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
           (SPC21) (SEC11-like 3)
          Length = 192

 Score =  295 bits (756), Expect = 6e-80
 Identities = 138/177 (77%), Positives = 162/177 (91%)
 Frame = +1

Query: 67  MSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFR 246
           + IF DL++MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF R
Sbjct: 16  LDIFGDLKKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHR 75

Query: 247 GDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDR 426
           GDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDR
Sbjct: 76  GDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDR 135

Query: 427 GLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
           GLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L  +G +VL  RES
Sbjct: 136 GLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192

>sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
           (SPC18) (Endopeptidase SP18) (SEC11-like 1)
          Length = 179

 Score =  295 bits (755), Expect = 7e-80
 Identities = 138/179 (77%), Positives = 163/179 (91%)
 Frame = +1

Query: 58  LFSMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPA 237
           + S+   DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+++TGSESPIVVVLSGSMEPA
Sbjct: 1   MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMLITGSESPIVVVLSGSMEPA 60

Query: 238 FFRGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSV 417
           F RGDLLFLTN  ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+V
Sbjct: 61  FHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAV 120

Query: 418 DDRGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 594
           DDRGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKF YA+L  LGLFVL HRE
Sbjct: 121 DDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFSYAVLFLLGLFVLVHRE 179

>sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11
          Length = 167

 Score =  149 bits (377), Expect = 5e-36
 Identities = 77/164 (46%), Positives = 110/164 (67%), Gaps = 1/164 (0%)
 Frame = +1

Query: 94  MNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNF 273
           MN R    ++LN   +  SA M W+GL + T S SPIVVVLSGSMEPAF RGD+LFL N 
Sbjct: 1   MNLRFELQKLLNVCFLFASAYMFWQGLAIATNSASPIVVVLSGSMEPAFQRGDILFLWN- 59

Query: 274 RDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYW 453
           R+   +VGD++V+ ++G++IPIVHRV++ H         LTKGDNN+ +D  LYA  + +
Sbjct: 60  RNTFNQVGDVVVYEVEGKQIPIVHRVLRQHNNHADKQFLLTKGDNNAGNDISLYANKKIY 119

Query: 454 LER-KDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVL 582
           L + K+++G  KG+ P +G +TI +++    K+A+LG LGL  L
Sbjct: 120 LNKSKEIVGTVKGYFPQLGYITIWISENKYAKFALLGMLGLSAL 163

>sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader peptidase I)
          Length = 190

 Score = 51.6 bits (122), Expect = 2e-06
 Identities = 42/140 (30%), Positives = 68/140 (48%), Gaps = 11/140 (7%)
 Frame = +1

Query: 121 VLNFSMIVCSALMIWKGLIVVTGSES---PIVV------VLSGSMEPAFFRGDLLFLTNF 273
           + N   ++   L+I   L+V++   S   P V       VLSGSMEP F  G L+ +   
Sbjct: 4   ISNILYVIIFTLIIVLTLVVISTRSSGGEPAVFGYTLKSVLSGSMEPEFNTGSLILVKEI 63

Query: 274 RD-EPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQY 450
            D + ++ GD+I F  D     + HR++ +  K+   + F TKGDNN+  D    ++   
Sbjct: 64  TDVKELQKGDVITFMQDA-NTAVTHRIVDI-TKQGDHLLFKTKGDNNAAADSAPVSD--- 118

Query: 451 WLERKDVIGRAKGF-MPYIG 507
               ++V  +  GF +PY G
Sbjct: 119 ----ENVRAQYTGFQLPYAG 134

>sp|Q58920|YF25_METJA Hypothetical protein MJ1525
          Length = 933

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 32/96 (33%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
 Frame = -2

Query: 581 NTNNPRQPSIAYLNFG*SFIMIVTIPIYGMNPLARPITSFLSNQY*FSAYNPLSSTELLS 402
           N  N    SI Y+ FG SF+++V I  YGM     PITS L      S Y   +    + 
Sbjct: 353 NLKNIVYLSIFYI-FG-SFVLLVAI--YGMGIAISPITSPLGYNQILSTYTQTTGWPNVY 408

Query: 401 PLVKNLIDPSSFS*TFI-TLCTIGISLPSILNTIIS 297
             V  L  PSS+S  F   + +  I++  IL  ++S
Sbjct: 409 TTVAELAKPSSWSEIFTNAIGSDTIAIVGILGILLS 444

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,275,603
Number of Sequences: 369166
Number of extensions: 1521711
Number of successful extensions: 3933
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3929
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5636246860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00174

  1. Dr_sW_005_F17
  2. Dr_sW_001_E22
  3. Dr_sW_010_M16
  4. Dr_sW_003_N03