Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_E22
(611 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa su... 298 7e-81
sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa su... 297 1e-80
sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subu... 297 2e-80
sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa su... 296 2e-80
sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa su... 295 5e-80
sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa su... 295 5e-80
sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subu... 294 1e-79
sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11 149 4e-36
sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader... 52 2e-06
sp|Q58920|YF25_METJA Hypothetical protein MJ1525 32 1.7
>sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 298 bits (763), Expect = 7e-81
Identities = 140/178 (78%), Positives = 163/178 (91%)
Frame = +3
Query: 3 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 182
S+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF
Sbjct: 15 SLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFH 74
Query: 183 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 362
RGDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDD
Sbjct: 75 RGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDD 134
Query: 363 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 536
RGLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 135 RGLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1) (Sid 2895)
Length = 179
Score = 297 bits (761), Expect = 1e-80
Identities = 140/177 (79%), Positives = 163/177 (92%)
Frame = +3
Query: 3 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 182
S+ DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPAF
Sbjct: 3 SLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPAFH 62
Query: 183 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 362
RGDLLFLTN ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+VDD
Sbjct: 63 RGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAVDD 122
Query: 363 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 533
RGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKFKYA+L LGLFVL HRE
Sbjct: 123 RGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179
>sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 297 bits (760), Expect = 2e-80
Identities = 139/178 (78%), Positives = 163/178 (91%)
Frame = +3
Query: 3 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 182
S+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF
Sbjct: 15 SLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFH 74
Query: 183 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 362
RGDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDD
Sbjct: 75 RGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDD 134
Query: 363 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 536
RGLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA++ +G +VL RES
Sbjct: 135 RGLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALVAVMGAYVLLKRES 192
>sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 296 bits (759), Expect = 2e-80
Identities = 139/177 (78%), Positives = 162/177 (91%)
Frame = +3
Query: 6 MSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFR 185
+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF R
Sbjct: 16 LDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHR 75
Query: 186 GDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDR 365
GDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDR
Sbjct: 76 GDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDR 135
Query: 366 GLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 536
GLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 136 GLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
sp|Q5RC30|SPC21_PONPY Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 295 bits (756), Expect = 5e-80
Identities = 138/177 (77%), Positives = 162/177 (91%)
Frame = +3
Query: 6 MSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFR 185
+ IF DL++MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF R
Sbjct: 16 LDIFGDLKKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHR 75
Query: 186 GDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDR 365
GDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDR
Sbjct: 76 GDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDR 135
Query: 366 GLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 536
GLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 136 GLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|P67811|SPC18_CANFA Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|P67810|SPC18_BOVIN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|Q5R9C7|SPC18_PONPY Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
Length = 179
Score = 295 bits (756), Expect = 5e-80
Identities = 139/177 (78%), Positives = 163/177 (92%)
Frame = +3
Query: 3 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 182
S+ DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPAF
Sbjct: 3 SLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPAFH 62
Query: 183 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 362
RGDLLFLTN ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK++G IKFLTKGDNN+VDD
Sbjct: 63 RGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAVDD 122
Query: 363 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 533
RGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKFKYA+L LGLFVL HRE
Sbjct: 123 RGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179
>sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
Length = 179
Score = 294 bits (753), Expect = 1e-79
Identities = 138/177 (77%), Positives = 162/177 (91%)
Frame = +3
Query: 3 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 182
S+ DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+++TGSESPIVVVLSGSMEPAF
Sbjct: 3 SLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMLITGSESPIVVVLSGSMEPAFH 62
Query: 183 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 362
RGDLLFLTN ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+VDD
Sbjct: 63 RGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAVDD 122
Query: 363 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 533
RGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKF YA+L LGLFVL HRE
Sbjct: 123 RGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFSYAVLFLLGLFVLVHRE 179
>sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11
Length = 167
Score = 149 bits (377), Expect = 4e-36
Identities = 77/164 (46%), Positives = 110/164 (67%), Gaps = 1/164 (0%)
Frame = +3
Query: 33 MNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNF 212
MN R ++LN + SA M W+GL + T S SPIVVVLSGSMEPAF RGD+LFL N
Sbjct: 1 MNLRFELQKLLNVCFLFASAYMFWQGLAIATNSASPIVVVLSGSMEPAFQRGDILFLWN- 59
Query: 213 RDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYW 392
R+ +VGD++V+ ++G++IPIVHRV++ H LTKGDNN+ +D LYA + +
Sbjct: 60 RNTFNQVGDVVVYEVEGKQIPIVHRVLRQHNNHADKQFLLTKGDNNAGNDISLYANKKIY 119
Query: 393 LER-KDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVL 521
L + K+++G KG+ P +G +TI +++ K+A+LG LGL L
Sbjct: 120 LNKSKEIVGTVKGYFPQLGYITIWISENKYAKFALLGMLGLSAL 163
>sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader peptidase I)
Length = 190
Score = 51.6 bits (122), Expect = 2e-06
Identities = 42/140 (30%), Positives = 68/140 (48%), Gaps = 11/140 (7%)
Frame = +3
Query: 60 VLNFSMIVCSALMIWKGLIVVTGSES---PIVV------VLSGSMEPAFFRGDLLFLTNF 212
+ N ++ L+I L+V++ S P V VLSGSMEP F G L+ +
Sbjct: 4 ISNILYVIIFTLIIVLTLVVISTRSSGGEPAVFGYTLKSVLSGSMEPEFNTGSLILVKEI 63
Query: 213 RD-EPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQY 389
D + ++ GD+I F D + HR++ + K+ + F TKGDNN+ D ++
Sbjct: 64 TDVKELQKGDVITFMQDA-NTAVTHRIVDI-TKQGDHLLFKTKGDNNAAADSAPVSD--- 118
Query: 390 WLERKDVIGRAKGF-MPYIG 446
++V + GF +PY G
Sbjct: 119 ----ENVRAQYTGFQLPYAG 134
>sp|Q58920|YF25_METJA Hypothetical protein MJ1525
Length = 933
Score = 31.6 bits (70), Expect = 1.7
Identities = 32/96 (33%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
Frame = -2
Query: 520 NTNNPRQPSIAYLNFG*SFIMIVTIPIYGMNPLARPITSFLSNQY*FSAYNPLSSTELLS 341
N N SI Y+ FG SF+++V I YGM PITS L S Y + +
Sbjct: 353 NLKNIVYLSIFYI-FG-SFVLLVAI--YGMGIAISPITSPLGYNQILSTYTQTTGWPNVY 408
Query: 340 PLVKNLIDPSSFS*TFI-TLCTIGISLPSILNTIIS 236
V L PSS+S F + + I++ IL ++S
Sbjct: 409 TTVAELAKPSSWSEIFTNAIGSDTIAIVGILGILLS 444
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,677,662
Number of Sequences: 369166
Number of extensions: 1395010
Number of successful extensions: 3693
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3689
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4797864890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)