Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_N03
(567 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa su... 270 1e-72
sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa su... 270 2e-72
sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa su... 270 2e-72
sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa su... 270 2e-72
sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subu... 269 4e-72
sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa su... 268 5e-72
sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subu... 267 1e-71
sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11 148 8e-36
sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader... 52 1e-06
sp|Q58920|YF25_METJA Hypothetical protein MJ1525 32 1.4
>sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1) (Sid 2895)
Length = 179
Score = 270 bits (691), Expect = 1e-72
Identities = 128/159 (80%), Positives = 148/159 (93%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPAF RGDLLFLTN ++PIRVG
Sbjct: 21 QVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPAFHRGDLLFLTNRVEDPIRVG 80
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+VDDRGLY + Q+WLE+KDV+G
Sbjct: 81 EIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAVDDRGLYKQGQHWLEKKDVVG 140
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 489
RA+GF+PYIG+VTI+MNDYPKFKYA+L LGLFVL HRE
Sbjct: 141 RARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179
>sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
sp|Q5RC30|SPC21_PONPY Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 270 bits (690), Expect = 2e-72
Identities = 127/160 (79%), Positives = 147/160 (91%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF RGDLLFLTNFR++PIR G
Sbjct: 33 QVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHRGDLLFLTNFREDPIRAG 92
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDRGLY E Q WLE+KDV+G
Sbjct: 93 EIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDRGLYKEGQNWLEKKDVVG 152
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 492
RA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 153 RARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 270 bits (690), Expect = 2e-72
Identities = 127/160 (79%), Positives = 147/160 (91%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF RGDLLFLTNFR++PIR G
Sbjct: 33 QVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHRGDLLFLTNFREDPIRAG 92
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDRGLY E Q WLE+KDV+G
Sbjct: 93 EIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDRGLYKEGQNWLEKKDVVG 152
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 492
RA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 153 RARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 270 bits (690), Expect = 2e-72
Identities = 127/160 (79%), Positives = 147/160 (91%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF RGDLLFLTNFR++PIR G
Sbjct: 33 QVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHRGDLLFLTNFREDPIRAG 92
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDRGLY E Q WLE+KDV+G
Sbjct: 93 EIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDRGLYKEGQNWLEKKDVVG 152
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 492
RA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 153 RARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 269 bits (687), Expect = 4e-72
Identities = 126/160 (78%), Positives = 147/160 (91%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF RGDLLFLTNFR++PIR G
Sbjct: 33 QVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHRGDLLFLTNFREDPIRAG 92
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDRGLY E Q WLE+KDV+G
Sbjct: 93 EIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDRGLYKEGQNWLEKKDVVG 152
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 492
RA+GF+PY+GMVTIIMNDYPKFKYA++ +G +VL RES
Sbjct: 153 RARGFLPYVGMVTIIMNDYPKFKYALVAVMGAYVLLKRES 192
>sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|P67811|SPC18_CANFA Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|P67810|SPC18_BOVIN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|Q5R9C7|SPC18_PONPY Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
Length = 179
Score = 268 bits (686), Expect = 5e-72
Identities = 127/159 (79%), Positives = 148/159 (93%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPAF RGDLLFLTN ++PIRVG
Sbjct: 21 QVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPAFHRGDLLFLTNRVEDPIRVG 80
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VFRI+GREIPIVHRV+K+HEK++G IKFLTKGDNN+VDDRGLY + Q+WLE+KDV+G
Sbjct: 81 EIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAVDDRGLYKQGQHWLEKKDVVG 140
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 489
RA+GF+PYIG+VTI+MNDYPKFKYA+L LGLFVL HRE
Sbjct: 141 RARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179
>sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
Length = 179
Score = 267 bits (683), Expect = 1e-71
Identities = 126/159 (79%), Positives = 147/159 (92%)
Frame = +1
Query: 13 QVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEPIRVG 192
QVLNF MIV SALMIWKGL+++TGSESPIVVVLSGSMEPAF RGDLLFLTN ++PIRVG
Sbjct: 21 QVLNFGMIVSSALMIWKGLMLITGSESPIVVVLSGSMEPAFHRGDLLFLTNRVEDPIRVG 80
Query: 193 DIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLERKDVIG 372
+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+VDDRGLY + Q+WLE+KDV+G
Sbjct: 81 EIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAVDDRGLYKQGQHWLEKKDVVG 140
Query: 373 RAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 489
RA+GF+PYIG+VTI+MNDYPKF YA+L LGLFVL HRE
Sbjct: 141 RARGFVPYIGIVTILMNDYPKFSYAVLFLLGLFVLVHRE 179
>sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11
Length = 167
Score = 148 bits (374), Expect = 8e-36
Identities = 75/160 (46%), Positives = 109/160 (68%), Gaps = 1/160 (0%)
Frame = +1
Query: 1 FGTRQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNFRDEP 180
F +++LN + SA M W+GL + T S SPIVVVLSGSMEPAF RGD+LFL N R+
Sbjct: 5 FELQKLLNVCFLFASAYMFWQGLAIATNSASPIVVVLSGSMEPAFQRGDILFLWN-RNTF 63
Query: 181 IRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYWLER- 357
+VGD++V+ ++G++IPIVHRV++ H LTKGDNN+ +D LYA + +L +
Sbjct: 64 NQVGDVVVYEVEGKQIPIVHRVLRQHNNHADKQFLLTKGDNNAGNDISLYANKKIYLNKS 123
Query: 358 KDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVL 477
K+++G KG+ P +G +TI +++ K+A+LG LGL L
Sbjct: 124 KEIVGTVKGYFPQLGYITIWISENKYAKFALLGMLGLSAL 163
>sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader peptidase I)
Length = 190
Score = 51.6 bits (122), Expect = 1e-06
Identities = 42/140 (30%), Positives = 68/140 (48%), Gaps = 11/140 (7%)
Frame = +1
Query: 16 VLNFSMIVCSALMIWKGLIVVTGSES---PIVV------VLSGSMEPAFFRGDLLFLTNF 168
+ N ++ L+I L+V++ S P V VLSGSMEP F G L+ +
Sbjct: 4 ISNILYVIIFTLIIVLTLVVISTRSSGGEPAVFGYTLKSVLSGSMEPEFNTGSLILVKEI 63
Query: 169 RD-EPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQY 345
D + ++ GD+I F D + HR++ + K+ + F TKGDNN+ D ++
Sbjct: 64 TDVKELQKGDVITFMQDA-NTAVTHRIVDI-TKQGDHLLFKTKGDNNAAADSAPVSD--- 118
Query: 346 WLERKDVIGRAKGF-MPYIG 402
++V + GF +PY G
Sbjct: 119 ----ENVRAQYTGFQLPYAG 134
>sp|Q58920|YF25_METJA Hypothetical protein MJ1525
Length = 933
Score = 31.6 bits (70), Expect = 1.4
Identities = 32/96 (33%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
Frame = -2
Query: 476 NTNNPRQPSIAYLNFG*SFIMIVTIPIYGMNPLARPITSFLSNQY*FSAYNPLSSTELLS 297
N N SI Y+ FG SF+++V I YGM PITS L S Y + +
Sbjct: 353 NLKNIVYLSIFYI-FG-SFVLLVAI--YGMGIAISPITSPLGYNQILSTYTQTTGWPNVY 408
Query: 296 PLVKNLIDPSSFS*TFI-TLCTIGISLPSILNTIIS 192
V L PSS+S F + + I++ IL ++S
Sbjct: 409 TTVAELAKPSSWSEIFTNAIGSDTIAIVGILGILLS 444
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,659,874
Number of Sequences: 369166
Number of extensions: 1320821
Number of successful extensions: 3530
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3526
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4127973360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)