Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_005_F17
(668 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa su... 298 9e-81
sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa su... 298 9e-81
sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subu... 297 2e-80
sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa su... 296 3e-80
sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa su... 296 3e-80
sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa su... 295 6e-80
sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subu... 295 7e-80
sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11 149 5e-36
sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader... 52 2e-06
sp|P37675|YIAN_ECOLI Hypothetical protein yiaN 32 1.5
>sp|Q9D8V7|SPC21_MOUSE Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 298 bits (763), Expect = 9e-81
Identities = 140/178 (78%), Positives = 163/178 (91%)
Frame = +1
Query: 64 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 243
S+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF
Sbjct: 15 SLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFH 74
Query: 244 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 423
RGDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDD
Sbjct: 75 RGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDD 134
Query: 424 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
RGLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 135 RGLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|Q9R0P6|SPC18_MOUSE Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1) (Sid 2895)
Length = 179
Score = 298 bits (763), Expect = 9e-81
Identities = 140/179 (78%), Positives = 164/179 (91%)
Frame = +1
Query: 58 LFSMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPA 237
+ S+ DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPA
Sbjct: 1 MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPA 60
Query: 238 FFRGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSV 417
F RGDLLFLTN ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+V
Sbjct: 61 FHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAV 120
Query: 418 DDRGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 594
DDRGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKFKYA+L LGLFVL HRE
Sbjct: 121 DDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179
>sp|Q9WTR7|SPC21_RAT Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 297 bits (760), Expect = 2e-80
Identities = 139/178 (78%), Positives = 163/178 (91%)
Frame = +1
Query: 64 SMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFF 243
S+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF
Sbjct: 15 SLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFH 74
Query: 244 RGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDD 423
RGDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDD
Sbjct: 75 RGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDD 134
Query: 424 RGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
RGLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA++ +G +VL RES
Sbjct: 135 RGLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALVAVMGAYVLLKRES 192
>sp|P13679|SPC21_CANFA Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 296 bits (759), Expect = 3e-80
Identities = 139/177 (78%), Positives = 162/177 (91%)
Frame = +1
Query: 67 MSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFR 246
+ IF DLR+MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF R
Sbjct: 16 LDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHR 75
Query: 247 GDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDR 426
GDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDR
Sbjct: 76 GDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDR 135
Query: 427 GLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
GLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 136 GLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|P67812|SPC18_HUMAN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|P67811|SPC18_CANFA Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|P67810|SPC18_BOVIN Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
sp|Q5R9C7|SPC18_PONPY Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
Length = 179
Score = 296 bits (758), Expect = 3e-80
Identities = 139/179 (77%), Positives = 164/179 (91%)
Frame = +1
Query: 58 LFSMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPA 237
+ S+ DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+V+TGSESPIVVVLSGSMEPA
Sbjct: 1 MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVITGSESPIVVVLSGSMEPA 60
Query: 238 FFRGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSV 417
F RGDLLFLTN ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK++G IKFLTKGDNN+V
Sbjct: 61 FHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAV 120
Query: 418 DDRGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 594
DDRGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKFKYA+L LGLFVL HRE
Sbjct: 121 DDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE 179
>sp|Q9BY50|SPC21_HUMAN Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
sp|Q5RC30|SPC21_PONPY Microsomal signal peptidase 21 kDa subunit (SPase 21 kDa subunit)
(SPC21) (SEC11-like 3)
Length = 192
Score = 295 bits (756), Expect = 6e-80
Identities = 138/177 (77%), Positives = 162/177 (91%)
Frame = +1
Query: 67 MSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFR 246
+ IF DL++MNKRQ+YYQVLNF+MIV SALMIWKGLIV+TGSESPIVVVLSGSMEPAF R
Sbjct: 16 LDIFGDLKKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSMEPAFHR 75
Query: 247 GDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDR 426
GDLLFLTNFR++PIR G+I+VF+++GR+IPIVHRVIKVHEK++G IKFLTKGDNN VDDR
Sbjct: 76 GDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDR 135
Query: 427 GLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRES 597
GLY E Q WLE+KDV+GRA+GF+PY+GMVTIIMNDYPKFKYA+L +G +VL RES
Sbjct: 136 GLYKEGQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES 192
>sp|P42667|SPC18_RAT Microsomal signal peptidase 18 kDa subunit (SPase 18 kDa subunit)
(SPC18) (Endopeptidase SP18) (SEC11-like 1)
Length = 179
Score = 295 bits (755), Expect = 7e-80
Identities = 138/179 (77%), Positives = 163/179 (91%)
Frame = +1
Query: 58 LFSMSIFDDLRRMNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPA 237
+ S+ DD+RRMNKRQ+YYQVLNF MIV SALMIWKGL+++TGSESPIVVVLSGSMEPA
Sbjct: 1 MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMLITGSESPIVVVLSGSMEPA 60
Query: 238 FFRGDLLFLTNFRDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSV 417
F RGDLLFLTN ++PIRVG+I+VFRI+GREIPIVHRV+K+HEK+DG IKFLTKGDNN+V
Sbjct: 61 FHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQDGHIKFLTKGDNNAV 120
Query: 418 DDRGLYAENQYWLERKDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVLAHRE 594
DDRGLY + Q+WLE+KDV+GRA+GF+PYIG+VTI+MNDYPKF YA+L LGLFVL HRE
Sbjct: 121 DDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFSYAVLFLLGLFVLVHRE 179
>sp|P15367|SEC11_YEAST Signal sequence processing protein SEC11
Length = 167
Score = 149 bits (377), Expect = 5e-36
Identities = 77/164 (46%), Positives = 110/164 (67%), Gaps = 1/164 (0%)
Frame = +1
Query: 94 MNKRQVYYQVLNFSMIVCSALMIWKGLIVVTGSESPIVVVLSGSMEPAFFRGDLLFLTNF 273
MN R ++LN + SA M W+GL + T S SPIVVVLSGSMEPAF RGD+LFL N
Sbjct: 1 MNLRFELQKLLNVCFLFASAYMFWQGLAIATNSASPIVVVLSGSMEPAFQRGDILFLWN- 59
Query: 274 RDEPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQYW 453
R+ +VGD++V+ ++G++IPIVHRV++ H LTKGDNN+ +D LYA + +
Sbjct: 60 RNTFNQVGDVVVYEVEGKQIPIVHRVLRQHNNHADKQFLLTKGDNNAGNDISLYANKKIY 119
Query: 454 LER-KDVIGRAKGFMPYIGMVTIIMNDYPKFKYAMLGCLGLFVL 582
L + K+++G KG+ P +G +TI +++ K+A+LG LGL L
Sbjct: 120 LNKSKEIVGTVKGYFPQLGYITIWISENKYAKFALLGMLGLSAL 163
>sp|P54506|LEPW_BACSU Signal peptidase I W (SPase I) (Leader peptidase I)
Length = 190
Score = 51.6 bits (122), Expect = 2e-06
Identities = 42/140 (30%), Positives = 68/140 (48%), Gaps = 11/140 (7%)
Frame = +1
Query: 121 VLNFSMIVCSALMIWKGLIVVTGSES---PIVV------VLSGSMEPAFFRGDLLFLTNF 273
+ N ++ L+I L+V++ S P V VLSGSMEP F G L+ +
Sbjct: 4 ISNILYVIIFTLIIVLTLVVISTRSSGGEPAVFGYTLKSVLSGSMEPEFNTGSLILVKEI 63
Query: 274 RD-EPIRVGDIIVFRIDGREIPIVHRVIKVHEKEDGSIKFLTKGDNNSVDDRGLYAENQY 450
D + ++ GD+I F D + HR++ + K+ + F TKGDNN+ D ++
Sbjct: 64 TDVKELQKGDVITFMQDA-NTAVTHRIVDI-TKQGDHLLFKTKGDNNAAADSAPVSD--- 118
Query: 451 WLERKDVIGRAKGF-MPYIG 507
++V + GF +PY G
Sbjct: 119 ----ENVRAQYTGFQLPYAG 134
>sp|P37675|YIAN_ECOLI Hypothetical protein yiaN
Length = 425
Score = 32.0 bits (71), Expect = 1.5
Identities = 18/39 (46%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = -1
Query: 416 TELLSPLVKNL-IDPSSFS*TFITRCTIGISLPSILNTI 303
T +L PLVK IDP F FI C+IG+ P I N +
Sbjct: 340 TPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVL 378
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,433,388
Number of Sequences: 369166
Number of extensions: 1558273
Number of successful extensions: 3867
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3770
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3863
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5657676120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)