Planaria EST Database


DrC_01105

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01105
         (923 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O75689|CENA1_HUMAN  Centaurin-alpha 1 (Putative MAPK-acti...   135   2e-31
sp|Q9NPF8|CENA2_HUMAN  Centaurin-alpha 2                          110   6e-24
sp|Q9JK15|CENA2_RAT  Centaurin-alpha 2                            105   2e-22
sp|Q8R2V5|CENA2_MOUSE  Centaurin-alpha 2                          105   2e-22
sp|Q7TQG1|PLEA6_MOUSE  Pleckstrin homology domain-containing...    43   0.001
sp|P60669|PLEA4_RAT  Pleckstrin homology domain-containing p...    42   0.002
sp|Q9H4M7|PLEA4_HUMAN  Pleckstrin homology domain-containing...    42   0.002
sp|Q8VC98|PLEA4_MOUSE  Pleckstrin homology domain-containing...    42   0.002
sp|Q9Y2H5|PLEA6_HUMAN  Pleckstrin homology domain-containing...    41   0.005
sp|P79114|MYO10_BOVIN  Myosin-10 (Myosin X)                        39   0.027
>sp|O75689|CENA1_HUMAN Centaurin-alpha 1 (Putative MAPK-activating protein PM25)
          Length = 374

 Score =  135 bits (339), Expect = 2e-31
 Identities = 86/293 (29%), Positives = 146/293 (49%), Gaps = 4/293 (1%)
 Frame = +1

Query: 13  LPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLLKKRCKDKNIYNA 192
           +P FY  P  +  ++LR  +I AKY+ ++F   E   PY    + G L KR +D   + +
Sbjct: 89  VPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAGYREGFLWKRGRDNGQFLS 148

Query: 193 RFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMISFF 360
           R F L+  +    YF +    +PK  ++++ +NATF    I +P          L +++ 
Sbjct: 149 RKFVLTEREGALKYFNRNDAKEPKAVMKIEHLNATFQPAKIGHPHG--------LQVTYL 200

Query: 361 RQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLDIT 540
           +  + S R+I++     +++VDW+  +R A+F     Y+      A++ +L   L+ +  
Sbjct: 201 K--DNSTRNIFIYHEDGKEIVDWFNALRAARFH----YLQVAFPGASDADLVPKLSRNYL 254

Query: 541 MSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSSPP 720
             G+M KTG + TE ++KRW  +  RRL+Y   PL    +GE+ IG +  GY  +   PP
Sbjct: 255 KEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFPP 314

Query: 721 NARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMSVREMA 879
           + +        +TI TP R +LFA ++E D  E W+       D  M  +E A
Sbjct: 315 STQGHHWPHG-ITIVTPDRKFLFACETESDQRE-WVAAFQKAVDRPMLPQEYA 365
>sp|Q9NPF8|CENA2_HUMAN Centaurin-alpha 2
          Length = 381

 Score =  110 bits (275), Expect = 6e-24
 Identities = 83/290 (28%), Positives = 142/290 (48%), Gaps = 6/290 (2%)
 Frame = +1

Query: 13  LPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLLKKRCKDKNIYNA 192
           +P FY +P AN   +L+  +I AKY+ ++F + + +   +   + G L KR +D + +  
Sbjct: 93  VPAFYYIPQANDCLVLKEQWIRAKYERREF-MADGETISLPGNREGFLWKRGRDNSQFLR 151

Query: 193 RFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMISFF 360
           R F L   +    YF KE    PK  + +  +NATF    I +P          L I++ 
Sbjct: 152 RKFVLLAREGLLKYFTKEQGKSPKAVISIKDLNATFQTEKIGHPHG--------LQITYR 203

Query: 361 RQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLDIT 540
           R  +   R++++   + +++VDW+  +R A    R  Y+     E  E EL   LT +  
Sbjct: 204 R--DGHTRNLFVYHESGKEIVDWFNALRAA----RLQYLKMAFPELPESELVPFLTRNYL 257

Query: 541 MSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSS 714
             G+M KTG +  E ++KRW  L    RRLLY   PL    +G++ +G + +GY A    
Sbjct: 258 KQGFMEKTGPKQKEPFKKRWFALDCHERRLLYYKNPLDAFEQGQVFLGNKEQGYEAYEDL 317

Query: 715 PPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 864
           P   R     ++ LTI TP R ++    SE +  ++WL+ +  +  + ++
Sbjct: 318 PKGIRGNRW-KAGLTIVTPERRFVLTCPSEKE-QQEWLESLRGVLSSPLT 365
>sp|Q9JK15|CENA2_RAT Centaurin-alpha 2
          Length = 376

 Score =  105 bits (262), Expect = 2e-22
 Identities = 80/290 (27%), Positives = 140/290 (48%), Gaps = 6/290 (2%)
 Frame = +1

Query: 13  LPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLLKKRCKDKNIYNA 192
           +P FY +P A+   +L+  +I AKY+ ++F  ++   P     + G L KR +D + +  
Sbjct: 93  VPTFYYVPQASDCLVLKEQWIRAKYERQEFMAEKAVSP--PGDREGFLWKRGRDNSQFLR 150

Query: 193 RFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMISFF 360
           R F L   +    Y+ KE    PK  + +  +NATF    I +P          L I++ 
Sbjct: 151 RRFVLLSREGLLKYYTKEEGKTPKAIINIKDLNATFQTEKIGHPHG--------LQITY- 201

Query: 361 RQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLDIT 540
            ++E   R++++  ++ +++VDW+  +R A    R  Y+     +  E EL   +T +  
Sbjct: 202 -RKEGQTRNLFVYHDSGKEIVDWFNALRAA----RLQYLKLAFPDLPESELVPLITRNYL 256

Query: 541 MSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSS 714
             G+M KTG +  E ++KRW  L    RRLLY   PL    +G++ +G   +GY      
Sbjct: 257 KQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLDAFEQGQVFLGSNEQGYEVWEGL 316

Query: 715 PPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 864
           P   R     +  LT+ TP R ++F   +E +  E WL+ +  +  + +S
Sbjct: 317 PQGIRGNRW-KVGLTVITPERKFVFTCPTEKEQRE-WLESLRGVLSSPLS 364
>sp|Q8R2V5|CENA2_MOUSE Centaurin-alpha 2
          Length = 381

 Score =  105 bits (261), Expect = 2e-22
 Identities = 83/292 (28%), Positives = 140/292 (47%), Gaps = 8/292 (2%)
 Frame = +1

Query: 13  LPIFYRLPNANGPEILRRSFIIAKYQLKQFSV--KEVDRPYITNQKLGLLKKRCKDKNIY 186
           +P FY +P AN   +L+  +I AKY+ ++F+   K V  P     + G L KR +D   +
Sbjct: 93  VPAFYYVPQANDCLVLKEQWIRAKYERQEFTAIDKAVSHP---GNREGFLWKRGRDNAQF 149

Query: 187 NARFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMIS 354
             R F L   +    Y+ KE    PK  + +  +NATF    I +P          L I+
Sbjct: 150 LRRRFVLLSREGLLKYYTKEEGKAPKAVISIKDLNATFQTEKIGHPHG--------LQIT 201

Query: 355 FFRQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLD 534
           +  ++E   R++++  ++ +++VDW+  +R A    R  Y+     +  E EL   +T +
Sbjct: 202 Y--RKEGHTRNLFVYHDSGKEIVDWFNALRAA----RLQYLKLAFPDLPESELVPLITRN 255

Query: 535 ITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVL 708
               G+M KTG +  E ++KRW  L    RRLLY   PL     G++ +G   +GY    
Sbjct: 256 YLKQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLDAFELGQVFLGSNEQGYEVWE 315

Query: 709 SSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 864
             P   R     ++ LT+ TP R ++F   +E +  E WL+ +  +  + +S
Sbjct: 316 DLPKGIRGNRW-KAGLTVITPERKFIFTCPTEKEQRE-WLESLRGVLSSPLS 365
>sp|Q7TQG1|PLEA6_MOUSE Pleckstrin homology domain-containing protein family A member 6
           (Phosphoinositol 3-phosphate-binding protein 3) (PEPP-3)
          Length = 1173

 Score = 42.7 bits (99), Expect = 0.001
 Identities = 37/141 (26%), Positives = 62/141 (43%)
 Frame = +1

Query: 439 IRFAKFSLRAAYVVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGR 618
           IR  + S +A   +  G  A+ ++  R+    +T +GW+YK  S   +QW KRW VL+ R
Sbjct: 33  IRATRTSRKA---IAFGKRAHSMK--RNPNAPVTKAGWLYKQASSGVKQWNKRWFVLVDR 87

Query: 619 RLLYSSGPLSPCGKGEIVIGERSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVD 798
            L Y          G I +   S    AV  S   +R          ++T    Y F+ +
Sbjct: 88  CLFYYKDEKQESILGSIPL--LSFRVAAVQPSDNISRKHTFKAEHAGVRT----YFFSAE 141

Query: 799 SEGDVAEKWLKCINDLADTEM 861
           S  +  E W++ + + A  ++
Sbjct: 142 SP-EEQEAWIQAMGEAARVQI 161
>sp|P60669|PLEA4_RAT Pleckstrin homology domain-containing protein family A member 4
           (Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
          Length = 779

 Score = 42.4 bits (98), Expect = 0.002
 Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
 Frame = +1

Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVI---GE 681
           L RD  L + + GW++K  S     W++RW VL G  L Y          G +++     
Sbjct: 47  LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSV 106

Query: 682 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 858
           R  G GA    P   R   T + P       R Y+ A D+  D+   WL+ +   +  E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGKASRAE 155
>sp|Q9H4M7|PLEA4_HUMAN Pleckstrin homology domain-containing protein family A member 4
           (Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
          Length = 779

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
 Frame = +1

Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGE--- 681
           L RD  L + + GW++K  S     W++RW VL G  L Y          G +++     
Sbjct: 47  LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYNI 106

Query: 682 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 858
           R  G GA    P   R   T + P       R Y+ A D+  D+   WL+ +   +  E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGRASRAE 155
>sp|Q8VC98|PLEA4_MOUSE Pleckstrin homology domain-containing protein family A member 4
           (Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
          Length = 588

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
 Frame = +1

Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVI---GE 681
           L RD  L + + GW++K  S     W++RW VL G  L Y          G +++     
Sbjct: 47  LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSV 106

Query: 682 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 858
           R  G GA    P   R   T + P       R Y+ A D+  D+   WL+ +   +  E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGRASRAE 155
>sp|Q9Y2H5|PLEA6_HUMAN Pleckstrin homology domain-containing protein family A member 6
           (Phosphoinositol 3-phosphate-binding protein 3) (PEPP-3)
          Length = 1048

 Score = 40.8 bits (94), Expect = 0.005
 Identities = 30/117 (25%), Positives = 51/117 (43%)
 Frame = +1

Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSR 690
           + R+    +T +GW++K  S   +QW KRW VL+ R L Y          G I +   S 
Sbjct: 52  MKRNPNAPVTKAGWLFKQASSGVKQWNKRWFVLVDRCLFYYKDEKEESILGSIPL--LSF 109

Query: 691 GYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEM 861
              AV  S   +R          ++T    Y F+ +S  +  E W++ + + A  ++
Sbjct: 110 RVAAVQPSDNISRKHTFKAEHAGVRT----YFFSAESP-EEQEAWIQAMGEAARVQI 161
>sp|P79114|MYO10_BOVIN Myosin-10 (Myosin X)
          Length = 2052

 Score = 38.5 bits (88), Expect = 0.027
 Identities = 31/123 (25%), Positives = 57/123 (46%), Gaps = 5/123 (4%)
 Frame = +1

Query: 547  GWMYKTG---SQLTEQ-WQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSS 714
            GW++K G   S L+ + W+KRW VL   +L+Y         KG + +    R    ++ +
Sbjct: 1211 GWLHKKGGGSSTLSRRNWKKRWFVLRQAKLMYFENDSEEKLKGTVEV----RAAKEIIDN 1266

Query: 715  PPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDL-ADTEMSVREMADYQL 891
                    + ++ + I    R +    +S  D A +W   ++ + A T+  +REM D Q 
Sbjct: 1267 -------TSKENGIDIIMADRTFHLIAESPED-ASQWFSVLSQVHASTDQEIREMHDEQA 1318

Query: 892  HPK 900
            +P+
Sbjct: 1319 NPQ 1321
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,465,425
Number of Sequences: 369166
Number of extensions: 2134752
Number of successful extensions: 4901
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4886
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9462723610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01105

  1. Dr_sW_028_B16
  2. Dr_sW_027_N01
  3. Dr_sW_025_J05