Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_N01
(870 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75689|CENA1_HUMAN Centaurin-alpha 1 (Putative MAPK-acti... 133 7e-31
sp|Q9NPF8|CENA2_HUMAN Centaurin-alpha 2 110 5e-24
sp|Q9JK15|CENA2_RAT Centaurin-alpha 2 105 2e-22
sp|Q8R2V5|CENA2_MOUSE Centaurin-alpha 2 105 2e-22
sp|Q7TQG1|PLEA6_MOUSE Pleckstrin homology domain-containing... 43 0.001
sp|P60669|PLEA4_RAT Pleckstrin homology domain-containing p... 42 0.002
sp|Q9H4M7|PLEA4_HUMAN Pleckstrin homology domain-containing... 42 0.002
sp|Q8VC98|PLEA4_MOUSE Pleckstrin homology domain-containing... 42 0.002
sp|Q9Y2H5|PLEA6_HUMAN Pleckstrin homology domain-containing... 41 0.005
sp|Q96P48|CEND2_HUMAN Centaurin-delta 2 (Cnt-d2) (Arf-GAP, ... 37 0.055
>sp|O75689|CENA1_HUMAN Centaurin-alpha 1 (Putative MAPK-activating protein PM25)
Length = 374
Score = 133 bits (334), Expect = 7e-31
Identities = 84/287 (29%), Positives = 143/287 (49%), Gaps = 4/287 (1%)
Frame = +1
Query: 13 LPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLLKKRCKDKNIYNA 192
+P FY P + ++LR +I AKY+ ++F E PY + G L KR +D + +
Sbjct: 89 VPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAGYREGFLWKRGRDNGQFLS 148
Query: 193 RFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMISFF 360
R F L+ + YF + +PK ++++ +NATF I +P L +++
Sbjct: 149 RKFVLTEREGALKYFNRNDAKEPKAVMKIEHLNATFQPAKIGHPHG--------LQVTYL 200
Query: 361 RQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLDIT 540
+ + S R+I++ +++VDW+ +R A+F Y+ A++ +L L+ +
Sbjct: 201 K--DNSTRNIFIYHEDGKEIVDWFNALRAARFH----YLQVAFPGASDADLVPKLSRNYL 254
Query: 541 MSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSSPP 720
G+M KTG + TE ++KRW + RRL+Y PL +GE+ IG + GY + PP
Sbjct: 255 KEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFPP 314
Query: 721 NARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEM 861
+ + +TI TP R +LFA ++E D E W+ D M
Sbjct: 315 STQGHHWPHG-ITIVTPDRKFLFACETESDQRE-WVAAFQKAVDRPM 359
>sp|Q9NPF8|CENA2_HUMAN Centaurin-alpha 2
Length = 381
Score = 110 bits (275), Expect = 5e-24
Identities = 83/290 (28%), Positives = 142/290 (48%), Gaps = 6/290 (2%)
Frame = +1
Query: 13 LPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLLKKRCKDKNIYNA 192
+P FY +P AN +L+ +I AKY+ ++F + + + + + G L KR +D + +
Sbjct: 93 VPAFYYIPQANDCLVLKEQWIRAKYERREF-MADGETISLPGNREGFLWKRGRDNSQFLR 151
Query: 193 RFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMISFF 360
R F L + YF KE PK + + +NATF I +P L I++
Sbjct: 152 RKFVLLAREGLLKYFTKEQGKSPKAVISIKDLNATFQTEKIGHPHG--------LQITYR 203
Query: 361 RQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLDIT 540
R + R++++ + +++VDW+ +R A R Y+ E E EL LT +
Sbjct: 204 R--DGHTRNLFVYHESGKEIVDWFNALRAA----RLQYLKMAFPELPESELVPFLTRNYL 257
Query: 541 MSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSS 714
G+M KTG + E ++KRW L RRLLY PL +G++ +G + +GY A
Sbjct: 258 KQGFMEKTGPKQKEPFKKRWFALDCHERRLLYYKNPLDAFEQGQVFLGNKEQGYEAYEDL 317
Query: 715 PPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 864
P R ++ LTI TP R ++ SE + ++WL+ + + + ++
Sbjct: 318 PKGIRGNRW-KAGLTIVTPERRFVLTCPSEKE-QQEWLESLRGVLSSPLT 365
>sp|Q9JK15|CENA2_RAT Centaurin-alpha 2
Length = 376
Score = 105 bits (262), Expect = 2e-22
Identities = 80/290 (27%), Positives = 140/290 (48%), Gaps = 6/290 (2%)
Frame = +1
Query: 13 LPIFYRLPNANGPEILRRSFIIAKYQLKQFSVKEVDRPYITNQKLGLLKKRCKDKNIYNA 192
+P FY +P A+ +L+ +I AKY+ ++F ++ P + G L KR +D + +
Sbjct: 93 VPTFYYVPQASDCLVLKEQWIRAKYERQEFMAEKAVSP--PGDREGFLWKRGRDNSQFLR 150
Query: 193 RFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMISFF 360
R F L + Y+ KE PK + + +NATF I +P L I++
Sbjct: 151 RRFVLLSREGLLKYYTKEEGKTPKAIINIKDLNATFQTEKIGHPHG--------LQITY- 201
Query: 361 RQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLDIT 540
++E R++++ ++ +++VDW+ +R A R Y+ + E EL +T +
Sbjct: 202 -RKEGQTRNLFVYHDSGKEIVDWFNALRAA----RLQYLKLAFPDLPESELVPLITRNYL 256
Query: 541 MSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSS 714
G+M KTG + E ++KRW L RRLLY PL +G++ +G +GY
Sbjct: 257 KQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLDAFEQGQVFLGSNEQGYEVWEGL 316
Query: 715 PPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 864
P R + LT+ TP R ++F +E + E WL+ + + + +S
Sbjct: 317 PQGIRGNRW-KVGLTVITPERKFVFTCPTEKEQRE-WLESLRGVLSSPLS 364
>sp|Q8R2V5|CENA2_MOUSE Centaurin-alpha 2
Length = 381
Score = 105 bits (261), Expect = 2e-22
Identities = 83/292 (28%), Positives = 140/292 (47%), Gaps = 8/292 (2%)
Frame = +1
Query: 13 LPIFYRLPNANGPEILRRSFIIAKYQLKQFSV--KEVDRPYITNQKLGLLKKRCKDKNIY 186
+P FY +P AN +L+ +I AKY+ ++F+ K V P + G L KR +D +
Sbjct: 93 VPAFYYVPQANDCLVLKEQWIRAKYERQEFTAIDKAVSHP---GNREGFLWKRGRDNAQF 149
Query: 187 NARFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARIPDELLMIS 354
R F L + Y+ KE PK + + +NATF I +P L I+
Sbjct: 150 LRRRFVLLSREGLLKYYTKEEGKAPKAVISIKDLNATFQTEKIGHPHG--------LQIT 201
Query: 355 FFRQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELELARDLTLD 534
+ ++E R++++ ++ +++VDW+ +R A R Y+ + E EL +T +
Sbjct: 202 Y--RKEGHTRNLFVYHDSGKEIVDWFNALRAA----RLQYLKLAFPDLPESELVPLITRN 255
Query: 535 ITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVL 708
G+M KTG + E ++KRW L RRLLY PL G++ +G +GY
Sbjct: 256 YLKQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLDAFELGQVFLGSNEQGYEVWE 315
Query: 709 SSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 864
P R ++ LT+ TP R ++F +E + E WL+ + + + +S
Sbjct: 316 DLPKGIRGNRW-KAGLTVITPERKFIFTCPTEKEQRE-WLESLRGVLSSPLS 365
>sp|Q7TQG1|PLEA6_MOUSE Pleckstrin homology domain-containing protein family A member 6
(Phosphoinositol 3-phosphate-binding protein 3) (PEPP-3)
Length = 1173
Score = 42.7 bits (99), Expect = 0.001
Identities = 37/141 (26%), Positives = 62/141 (43%)
Frame = +1
Query: 439 IRFAKFSLRAAYVVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGR 618
IR + S +A + G A+ ++ R+ +T +GW+YK S +QW KRW VL+ R
Sbjct: 33 IRATRTSRKA---IAFGKRAHSMK--RNPNAPVTKAGWLYKQASSGVKQWNKRWFVLVDR 87
Query: 619 RLLYSSGPLSPCGKGEIVIGERSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVD 798
L Y G I + S AV S +R ++T Y F+ +
Sbjct: 88 CLFYYKDEKQESILGSIPL--LSFRVAAVQPSDNISRKHTFKAEHAGVRT----YFFSAE 141
Query: 799 SEGDVAEKWLKCINDLADTEM 861
S + E W++ + + A ++
Sbjct: 142 SP-EEQEAWIQAMGEAARVQI 161
>sp|P60669|PLEA4_RAT Pleckstrin homology domain-containing protein family A member 4
(Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
Length = 779
Score = 42.4 bits (98), Expect = 0.002
Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
Frame = +1
Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVI---GE 681
L RD L + + GW++K S W++RW VL G L Y G +++
Sbjct: 47 LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSV 106
Query: 682 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 858
R G GA P R T + P R Y+ A D+ D+ WL+ + + E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGKASRAE 155
>sp|Q9H4M7|PLEA4_HUMAN Pleckstrin homology domain-containing protein family A member 4
(Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
Length = 779
Score = 42.0 bits (97), Expect = 0.002
Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
Frame = +1
Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGE--- 681
L RD L + + GW++K S W++RW VL G L Y G +++
Sbjct: 47 LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYNI 106
Query: 682 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 858
R G GA P R T + P R Y+ A D+ D+ WL+ + + E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGRASRAE 155
>sp|Q8VC98|PLEA4_MOUSE Pleckstrin homology domain-containing protein family A member 4
(Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
Length = 588
Score = 42.0 bits (97), Expect = 0.002
Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
Frame = +1
Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVI---GE 681
L RD L + + GW++K S W++RW VL G L Y G +++
Sbjct: 47 LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSV 106
Query: 682 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 858
R G GA P R T + P R Y+ A D+ D+ WL+ + + E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGRASRAE 155
>sp|Q9Y2H5|PLEA6_HUMAN Pleckstrin homology domain-containing protein family A member 6
(Phosphoinositol 3-phosphate-binding protein 3) (PEPP-3)
Length = 1048
Score = 40.8 bits (94), Expect = 0.005
Identities = 30/117 (25%), Positives = 51/117 (43%)
Frame = +1
Query: 511 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSR 690
+ R+ +T +GW++K S +QW KRW VL+ R L Y G I + S
Sbjct: 52 MKRNPNAPVTKAGWLFKQASSGVKQWNKRWFVLVDRCLFYYKDEKEESILGSIPL--LSF 109
Query: 691 GYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEM 861
AV S +R ++T Y F+ +S + E W++ + + A ++
Sbjct: 110 RVAAVQPSDNISRKHTFKAEHAGVRT----YFFSAESP-EEQEAWIQAMGEAARVQI 161
>sp|Q96P48|CEND2_HUMAN Centaurin-delta 2 (Cnt-d2) (Arf-GAP, Rho-GAP, ankyrin repeat and
pleckstrin homology domain-containing protein 1)
Length = 1210
Score = 37.4 bits (85), Expect = 0.055
Identities = 30/109 (27%), Positives = 49/109 (44%), Gaps = 8/109 (7%)
Frame = +1
Query: 535 ITMSGWMYKTGS--------QLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSR 690
++ SG++YKT S + E++ +RWCVL L Y + GEI R+
Sbjct: 504 VSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEI----RAS 559
Query: 691 GYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCI 837
+ PP+ + + R YLF ++S + A +W+KCI
Sbjct: 560 EIVCLAVPPPDTHGF--EHTFEVYTEGERLYLFGLES-AEQAHEWVKCI 605
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,319,575
Number of Sequences: 369166
Number of extensions: 2002629
Number of successful extensions: 4723
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4552
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4708
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8598109270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)