Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_J05
(761 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75689|CENA1_HUMAN Centaurin-alpha 1 (Putative MAPK-acti... 104 2e-22
sp|Q9NPF8|CENA2_HUMAN Centaurin-alpha 2 87 6e-17
sp|Q9JK15|CENA2_RAT Centaurin-alpha 2 82 2e-15
sp|Q8R2V5|CENA2_MOUSE Centaurin-alpha 2 80 6e-15
sp|Q7TQG1|PLEA6_MOUSE Pleckstrin homology domain-containing... 43 0.001
sp|P60669|PLEA4_RAT Pleckstrin homology domain-containing p... 42 0.001
sp|Q9H4M7|PLEA4_HUMAN Pleckstrin homology domain-containing... 42 0.002
sp|Q8VC98|PLEA4_MOUSE Pleckstrin homology domain-containing... 42 0.002
sp|Q9Y2H5|PLEA6_HUMAN Pleckstrin homology domain-containing... 41 0.004
sp|P79114|MYO10_BOVIN Myosin-10 (Myosin X) 39 0.020
>sp|O75689|CENA1_HUMAN Centaurin-alpha 1 (Putative MAPK-activating protein PM25)
Length = 374
Score = 104 bits (260), Expect = 2e-22
Identities = 69/243 (28%), Positives = 118/243 (48%), Gaps = 4/243 (1%)
Frame = +1
Query: 1 RCKDKNIYNARFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARI 168
R +D + +R F L+ + YF + +PK ++++ +NATF I +P
Sbjct: 139 RGRDNGQFLSRKFVLTEREGALKYFNRNDAKEPKAVMKIEHLNATFQPAKIGHPHG---- 194
Query: 169 PDELLMISFFRQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELE 348
L +++ + S R+I++ +++VDW+ +R A+F G A++ +
Sbjct: 195 ----LQVTYLKDN--STRNIFIYHEDGKEIVDWFNALRAARFHYLQVAFPG----ASDAD 244
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSR 528
L L+ + G+M KTG + TE ++KRW + RRL+Y PL +GE+ IG +
Sbjct: 245 LVPKLSRNYLKEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKES 304
Query: 529 GYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMSVR 708
GY + PP+ + +TI TP R +LFA ++E D E W+ D M +
Sbjct: 305 GYTVLHGFPPSTQGHHWPHG-ITIVTPDRKFLFACETESDQRE-WVAAFQKAVDRPMLPQ 362
Query: 709 EMA 717
E A
Sbjct: 363 EYA 365
>sp|Q9NPF8|CENA2_HUMAN Centaurin-alpha 2
Length = 381
Score = 86.7 bits (213), Expect = 6e-17
Identities = 68/240 (28%), Positives = 114/240 (47%), Gaps = 6/240 (2%)
Frame = +1
Query: 1 RCKDKNIYNARFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARI 168
R +D + + R F L + YF KE PK + + +NATF I +P
Sbjct: 142 RGRDNSQFLRRKFVLLAREGLLKYFTKEQGKSPKAVISIKDLNATFQTEKIGHPHG---- 197
Query: 169 PDELLMISFFRQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELE 348
L I++ R + R++++ + +++VDW+ +R A R Y+ E E E
Sbjct: 198 ----LQITYRR--DGHTRNLFVYHESGKEIVDWFNALRAA----RLQYLKMAFPELPESE 247
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGER 522
L LT + G+M KTG + E ++KRW L RRLLY PL +G++ +G +
Sbjct: 248 LVPFLTRNYLKQGFMEKTGPKQKEPFKKRWFALDCHERRLLYYKNPLDAFEQGQVFLGNK 307
Query: 523 SRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 702
+GY A P R ++ LTI TP R ++ SE + ++WL+ + + + ++
Sbjct: 308 EQGYEAYEDLPKGIRGNRW-KAGLTIVTPERRFVLTCPSEKE-QQEWLESLRGVLSSPLT 365
>sp|Q9JK15|CENA2_RAT Centaurin-alpha 2
Length = 376
Score = 82.0 bits (201), Expect = 2e-15
Identities = 65/240 (27%), Positives = 113/240 (47%), Gaps = 6/240 (2%)
Frame = +1
Query: 1 RCKDKNIYNARFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARI 168
R +D + + R F L + Y+ KE PK + + +NATF I +P
Sbjct: 141 RGRDNSQFLRRRFVLLSREGLLKYYTKEEGKTPKAIINIKDLNATFQTEKIGHPHG---- 196
Query: 169 PDELLMISFFRQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELE 348
L I++ ++E R++++ ++ +++VDW+ +R A R Y+ + E E
Sbjct: 197 ----LQITY--RKEGQTRNLFVYHDSGKEIVDWFNALRAA----RLQYLKLAFPDLPESE 246
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGER 522
L +T + G+M KTG + E ++KRW L RRLLY PL +G++ +G
Sbjct: 247 LVPLITRNYLKQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLDAFEQGQVFLGSN 306
Query: 523 SRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 702
+GY P R + LT+ TP R ++F +E + E WL+ + + + +S
Sbjct: 307 EQGYEVWEGLPQGIRGNRW-KVGLTVITPERKFVFTCPTEKEQRE-WLESLRGVLSSPLS 364
>sp|Q8R2V5|CENA2_MOUSE Centaurin-alpha 2
Length = 381
Score = 80.1 bits (196), Expect = 6e-15
Identities = 65/240 (27%), Positives = 112/240 (46%), Gaps = 6/240 (2%)
Frame = +1
Query: 1 RCKDKNIYNARFFRLSISDITFNYFIKETDADPKRNLRLDRINATF----IENPDSRARI 168
R +D + R F L + Y+ KE PK + + +NATF I +P
Sbjct: 142 RGRDNAQFLRRRFVLLSREGLLKYYTKEEGKAPKAVISIKDLNATFQTEKIGHPHG---- 197
Query: 169 PDELLMISFFRQEEASWRHIYLKSNTPEDLVDWYQTIRFAKFSLRAAYVVGTGDEANELE 348
L I++ ++E R++++ ++ +++VDW+ +R A R Y+ + E E
Sbjct: 198 ----LQITY--RKEGHTRNLFVYHDSGKEIVDWFNALRAA----RLQYLKLAFPDLPESE 247
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVL--LGRRLLYSSGPLSPCGKGEIVIGER 522
L +T + G+M KTG + E ++KRW L RRLLY PL G++ +G
Sbjct: 248 LVPLITRNYLKQGFMEKTGPKHREPFKKRWFALDPQERRLLYYKNPLDAFELGQVFLGSN 307
Query: 523 SRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEMS 702
+GY P R ++ LT+ TP R ++F +E + E WL+ + + + +S
Sbjct: 308 EQGYEVWEDLPKGIRGNRW-KAGLTVITPERKFIFTCPTEKEQRE-WLESLRGVLSSPLS 365
>sp|Q7TQG1|PLEA6_MOUSE Pleckstrin homology domain-containing protein family A member 6
(Phosphoinositol 3-phosphate-binding protein 3) (PEPP-3)
Length = 1173
Score = 42.7 bits (99), Expect = 0.001
Identities = 37/141 (26%), Positives = 62/141 (43%)
Frame = +1
Query: 277 IRFAKFSLRAAYVVGTGDEANELELARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGR 456
IR + S +A + G A+ ++ R+ +T +GW+YK S +QW KRW VL+ R
Sbjct: 33 IRATRTSRKA---IAFGKRAHSMK--RNPNAPVTKAGWLYKQASSGVKQWNKRWFVLVDR 87
Query: 457 RLLYSSGPLSPCGKGEIVIGERSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVD 636
L Y G I + S AV S +R ++T Y F+ +
Sbjct: 88 CLFYYKDEKQESILGSIPL--LSFRVAAVQPSDNISRKHTFKAEHAGVRT----YFFSAE 141
Query: 637 SEGDVAEKWLKCINDLADTEM 699
S + E W++ + + A ++
Sbjct: 142 SP-EEQEAWIQAMGEAARVQI 161
>sp|P60669|PLEA4_RAT Pleckstrin homology domain-containing protein family A member 4
(Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
Length = 779
Score = 42.4 bits (98), Expect = 0.001
Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
Frame = +1
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVI---GE 519
L RD L + + GW++K S W++RW VL G L Y G +++
Sbjct: 47 LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSV 106
Query: 520 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 696
R G GA P R T + P R Y+ A D+ D+ WL+ + + E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGKASRAE 155
>sp|Q9H4M7|PLEA4_HUMAN Pleckstrin homology domain-containing protein family A member 4
(Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
Length = 779
Score = 42.0 bits (97), Expect = 0.002
Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
Frame = +1
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGE--- 519
L RD L + + GW++K S W++RW VL G L Y G +++
Sbjct: 47 LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYNI 106
Query: 520 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 696
R G GA P R T + P R Y+ A D+ D+ WL+ + + E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGRASRAE 155
>sp|Q8VC98|PLEA4_MOUSE Pleckstrin homology domain-containing protein family A member 4
(Phosphoinositol 3-phosphate binding protein 1) (PEPP-1)
Length = 588
Score = 42.0 bits (97), Expect = 0.002
Identities = 33/119 (27%), Positives = 49/119 (41%), Gaps = 3/119 (2%)
Frame = +1
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVI---GE 519
L RD L + + GW++K S W++RW VL G L Y G +++
Sbjct: 47 LRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSV 106
Query: 520 RSRGYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTE 696
R G GA P R T + P R Y+ A D+ D+ WL+ + + E
Sbjct: 107 RPDGPGA----PRGRRFTFTAEHP-----GMRTYVLAADTLEDL-RGWLRALGRASRAE 155
>sp|Q9Y2H5|PLEA6_HUMAN Pleckstrin homology domain-containing protein family A member 6
(Phosphoinositol 3-phosphate-binding protein 3) (PEPP-3)
Length = 1048
Score = 40.8 bits (94), Expect = 0.004
Identities = 30/117 (25%), Positives = 51/117 (43%)
Frame = +1
Query: 349 LARDLTLDITMSGWMYKTGSQLTEQWQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSR 528
+ R+ +T +GW++K S +QW KRW VL+ R L Y G I + S
Sbjct: 52 MKRNPNAPVTKAGWLFKQASSGVKQWNKRWFVLVDRCLFYYKDEKEESILGSIPL--LSF 109
Query: 529 GYGAVLSSPPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDLADTEM 699
AV S +R ++T Y F+ +S + E W++ + + A ++
Sbjct: 110 RVAAVQPSDNISRKHTFKAEHAGVRT----YFFSAESP-EEQEAWIQAMGEAARVQI 161
>sp|P79114|MYO10_BOVIN Myosin-10 (Myosin X)
Length = 2052
Score = 38.5 bits (88), Expect = 0.020
Identities = 31/123 (25%), Positives = 57/123 (46%), Gaps = 5/123 (4%)
Frame = +1
Query: 385 GWMYKTG---SQLTEQ-WQKRWCVLLGRRLLYSSGPLSPCGKGEIVIGERSRGYGAVLSS 552
GW++K G S L+ + W+KRW VL +L+Y KG + + R ++ +
Sbjct: 1211 GWLHKKGGGSSTLSRRNWKKRWFVLRQAKLMYFENDSEEKLKGTVEV----RAAKEIIDN 1266
Query: 553 PPNARSVMTDQSPLTIKTPSRDYLFAVDSEGDVAEKWLKCINDL-ADTEMSVREMADYQL 729
+ ++ + I R + +S D A +W ++ + A T+ +REM D Q
Sbjct: 1267 -------TSKENGIDIIMADRTFHLIAESPED-ASQWFSVLSQVHASTDQEIREMHDEQA 1318
Query: 730 HPK 738
+P+
Sbjct: 1319 NPQ 1321
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,641,571
Number of Sequences: 369166
Number of extensions: 1753922
Number of successful extensions: 4117
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3976
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4105
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)