Planaria EST Database

DrC_01070

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01070
         (564 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O74777|MIS3_SCHPO  Ribosomal RNA assembly protein mis3          56   5e-08
sp|Q57885|Y443_METJA  Hypothetical protein MJ0443                  54   3e-07
sp|P26538|VL2_HPV58  Minor capsid protein L2                       33   0.64 
sp|Q9UGJ0|AAKG2_HUMAN  5'-AMP-activated protein kinase, gamm...    32   1.4  
sp|Q82570|RRP2_IAFOM  RNA-directed RNA polymerase subunit P2...    31   1.9  
sp|P36284|COAT_SMSV1  Capsid protein precursor (Coat protein)      30   4.1  
sp|Q8RDN0|TGT_FUSNN  Queuine tRNA-ribosyltransferase (tRNA-g...    30   5.4  
sp|P38308|YB53_YEAST  Hypothetical 104.5 kDa protein in CDC4...    29   7.0  
sp|O83823|Y851_TREPA  Hypothetical protein TP0851                  29   7.0  
sp|O34204|TOP1_FERIS  DNA topoisomerase I (Omega-protein) (R...    29   7.0  

>sp|O74777|MIS3_SCHPO Ribosomal RNA assembly protein mis3
          Length = 327

 Score = 56.2 bits (134), Expect = 5e-08
 Identities = 39/144 (27%), Positives = 66/144 (45%)
 Frame = +2

Query: 83  PVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQKGIDFLNAF 262
           P  R K L+E W  + + L K   + C  D  +  +TV     + D   +    D +   
Sbjct: 59  PKYREKYLREVWPHVTRALDK-FGITCVLDLVEGSMTVKTTRKTFDPYSILDARDLIKLL 117

Query: 263 SKGFDFIDCVSLIRMDGLYIRTMYIKDIKRTIKGDHFTRAVGRMCGANGIMRKKIENATR 442
           ++   F   V +++ DG+    + I +I R    + F +   R+ G NG   K +E  T+
Sbjct: 118 ARSVPFPQAVKIMQ-DGVACDIIKIGNILRN--KERFVKRRQRLIGTNGQTLKALELLTQ 174

Query: 443 CRIVINETTVHIMGTYRGIDAAHR 514
           C I++  TTV +MG Y+G+    R
Sbjct: 175 CYILVQGTTVAVMGGYKGLKEVRR 198

>sp|Q57885|Y443_METJA Hypothetical protein MJ0443
          Length = 227

 Score = 53.5 bits (127), Expect = 3e-07
 Identities = 41/156 (26%), Positives = 73/156 (46%)
 Frame = +2

Query: 74  IKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQKGIDFL 253
           +KIP  R+  L  K  ++ K + KEL ++ + D     +T+Y      D + + K  D +
Sbjct: 19  LKIPKDRVGVLIGKKGNVKKTIEKELGVKLEID-ADGTVTIYGTDKQKDPLAVWKARDIV 77

Query: 254 NAFSKGFDFIDCVSLIRMDGLYIRTMYIKDIKRTIKGDHFTRAVGRMCGANGIMRKKIEN 433
            A  +GF+    + L+  D   +  + I+D   +   +   R  GR+ G  G  R+ IE+
Sbjct: 78  RAIGRGFNPEIALKLV-SDEYVLEVIDIEDYASS--DNSIRRLKGRVIGKEGKSRRYIES 134

Query: 434 ATRCRIVINETTVHIMGTYRGIDAAHRKLGSLVRGA 541
            T   + +   TV I+G +  +  A   +  L+RGA
Sbjct: 135 LTGANVSVYGNTVAIVGEHEPVQIAKEAVEMLLRGA 170

>sp|P26538|VL2_HPV58 Minor capsid protein L2
          Length = 472

 Score = 32.7 bits (73), Expect = 0.64
 Identities = 20/67 (29%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
 Frame = -3

Query: 529 QRSKFSVSSINTSIGSHNMNSCFIN-YDSTTSCIFNF----FSHNTISPTHSTNSSGKVI 365
           Q+ +F + S+NTS+  +++N    + Y      I +F     SH + + T ++N S   I
Sbjct: 340 QQQQFELQSLNTSVSPYSINDGLYDIYADDADTIHDFQSPLHSHTSFATTRTSNVS---I 396

Query: 364 PFNSAFD 344
           P N+ FD
Sbjct: 397 PLNTGFD 403

>sp|Q9UGJ0|AAKG2_HUMAN 5'-AMP-activated protein kinase, gamma-2 subunit (AMPK gamma-2
           chain) (AMPK gamma2) (H91620p)
          Length = 569

 Score = 31.6 bits (70), Expect = 1.4
 Identities = 18/53 (33%), Positives = 27/53 (50%)
 Frame = -3

Query: 490 IGSHNMNSCFINYDSTTSCIFNFFSHNTISPTHSTNSSGKVIPFNSAFDIFNI 332
           IG+++ N  FI+ D+      N F    IS     + SGKV+   S FD+ N+
Sbjct: 430 IGTYH-NIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINL 481

>sp|Q82570|RRP2_IAFOM RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 31.2 bits (69), Expect = 1.9
 Identities = 14/64 (21%), Positives = 33/64 (51%)
 Frame = +2

Query: 56  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 235
           N  +    +   RL+P   KW+    + + ++ L+    P  RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRSAIAPVSRPMFLYVRTNGTSKIKMK 576

Query: 236 KGID 247
            G++
Sbjct: 577 WGME 580

>sp|P36284|COAT_SMSV1 Capsid protein precursor (Coat protein)
          Length = 702

 Score = 30.0 bits (66), Expect = 4.1
 Identities = 18/81 (22%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
 Frame = -3

Query: 550 VYRRSTYQRSKFSVSSINTSI-GSHNMNSCFINYDSTTSCIFNFFSHNTISPTHSTNSSG 374
           +    T+ +  + + ++ T + GS N+        +  + + N+   NTI+PTH   S+ 
Sbjct: 478 ITNNGTHFKGFYIMGNLTTKVKGSDNLGETQQTSRTLFASVGNYKDQNTINPTHKITSNS 537

Query: 373 KVIPFNSAFDIFNIHRSNIKT 311
            V+     +D  N+  +  KT
Sbjct: 538 LVV-----YDANNVSAATAKT 553

>sp|Q8RDN0|TGT_FUSNN Queuine tRNA-ribosyltransferase (tRNA-guanine transglycosylase)
           (Guanine insertion enzyme)
          Length = 373

 Score = 29.6 bits (65), Expect = 5.4
 Identities = 15/44 (34%), Positives = 23/44 (52%)
 Frame = +2

Query: 242 IDFLNAFSKGFDFIDCVSLIRMDGLYIRTMYIKDIKRTIKGDHF 373
           +D LNA   G D +DCV   R+      T++ KD +  IK + +
Sbjct: 255 VDMLNAVESGIDMMDCVQPTRL--ARHGTVFTKDGRLVIKSERY 296

>sp|P38308|YB53_YEAST Hypothetical 104.5 kDa protein in CDC47-KTR3 intergenic region
          Length = 924

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 16/31 (51%), Positives = 20/31 (64%)
 Frame = -3

Query: 529 QRSKFSVSSINTSIGSHNMNSCFINYDSTTS 437
           QRS  SV+SI TSI S   N+  ++  STTS
Sbjct: 390 QRSNSSVASITTSIMSSIYNTSHVSLSSTTS 420

>sp|O83823|Y851_TREPA Hypothetical protein TP0851
          Length = 724

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
 Frame = -3

Query: 454 YDSTTSCIFNFFSHNTISPTHS-TNSSGKVIPFNSAFDIF-NIHRSNIKTIH 305
           ++ TT CI +F SH+  + TH+  N  G+++P +    +  NI     K+ H
Sbjct: 557 FEQTTRCISHFTSHDPKTITHNWVNMGGQLVPEDKFETLLQNIETGKFKSWH 608

>sp|O34204|TOP1_FERIS DNA topoisomerase I (Omega-protein) (Relaxing enzyme) (Untwisting
           enzyme) (Swivelase)
          Length = 699

 Score = 29.3 bits (64), Expect = 7.0
 Identities = 13/48 (27%), Positives = 26/48 (54%)
 Frame = +2

Query: 11  IINFYLFLKMPNNFDNLKTRNIKIPVTRLKPLKEKWDDIVKILVKELN 154
           ++N YL  + P+  D   T  ++  +  ++  K+ W ++VK  +KE N
Sbjct: 533 VVNHYLEQRFPDIVDKGFTAEMEKELDEVENGKKDWKEVVKSFLKEFN 580

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,885,818
Number of Sequences: 369166
Number of extensions: 1138047
Number of successful extensions: 3075
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3006
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3074
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4078830820
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01070

  1. Dr_sW_023_J20
  2. Dr_sW_026_N18
  3. Dr_sW_011_J15