Planarian EST Database


Dr_sW_023_J20

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_023_J20
         (330 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q57885|Y443_METJA  Hypothetical protein MJ0443                  32   0.58 
sp|Q82570|RRP2_IAFOM  RNA-directed RNA polymerase subunit P2...    31   0.76 
sp|O34204|TOP1_FERIS  DNA topoisomerase I (Omega-protein) (R...    29   2.9  
sp|O46895|RK4_GUITH  Chloroplast 50S ribosomal protein L4          29   3.8  
sp|P13175|RRP2_IAZI1  RNA-directed RNA polymerase subunit P2...    29   3.8  
sp|P13172|RRP2_IARUD  RNA-directed RNA polymerase subunit P2...    29   3.8  
sp|P15659|RRP2_IAWIL  RNA-directed RNA polymerase subunit P2...    28   4.9  
sp|P13178|RRP2_IATKM  RNA-directed RNA polymerase subunit P2...    28   4.9  
sp|P13174|RRP2_IAZH3  RNA-directed RNA polymerase subunit P2...    28   4.9  
sp|P21427|RRP2_IAANN  RNA-directed RNA polymerase subunit P2...    28   4.9  
>sp|Q57885|Y443_METJA Hypothetical protein MJ0443
          Length = 227

 Score = 31.6 bits (70), Expect = 0.58
 Identities = 19/68 (27%), Positives = 34/68 (50%)
 Frame = +2

Query: 68  IKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQKGIDFL 247
           +KIP  R+  L  K  ++ K + KEL ++ + D     +T+Y      D + + K  D +
Sbjct: 19  LKIPKDRVGVLIGKKGNVKKTIEKELGVKLEID-ADGTVTIYGTDKQKDPLAVWKARDIV 77

Query: 248 NAFSKGFD 271
            A  +GF+
Sbjct: 78  RAIGRGFN 85
>sp|Q82570|RRP2_IAFOM RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 31.2 bits (69), Expect = 0.76
 Identities = 14/64 (21%), Positives = 33/64 (51%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + + ++ L+    P  RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRSAIAPVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
>sp|O34204|TOP1_FERIS DNA topoisomerase I (Omega-protein) (Relaxing enzyme) (Untwisting
           enzyme) (Swivelase)
          Length = 699

 Score = 29.3 bits (64), Expect = 2.9
 Identities = 13/48 (27%), Positives = 26/48 (54%)
 Frame = +2

Query: 5   IINFYLFLKMPNNFDNLKTRNIKIPVTRLKPLKEKWDDIVKILVKELN 148
           ++N YL  + P+  D   T  ++  +  ++  K+ W ++VK  +KE N
Sbjct: 533 VVNHYLEQRFPDIVDKGFTAEMEKELDEVENGKKDWKEVVKSFLKEFN 580
>sp|O46895|RK4_GUITH Chloroplast 50S ribosomal protein L4
          Length = 223

 Score = 28.9 bits (63), Expect = 3.8
 Identities = 24/95 (25%), Positives = 41/95 (43%), Gaps = 4/95 (4%)
 Frame = +2

Query: 20  LFLKMPN----NFDNLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLT 187
           L+LK  N    N DNL   N+K      + L  K  ++++   K+  +   A+PT   L 
Sbjct: 124 LYLKSNNTKVINLDNLDFTNLKT-----RDLVIKCSNLIENYKKDQKILFVAEPTASGLW 178

Query: 188 VYLRSPSNDEVKLQKGIDFLNAFSKGFDFIDCVSL 292
            Y+++ SN ++    G+D             C +L
Sbjct: 179 RYVKNISNVDLIYTTGLDLKKILQAHHIIFTCKAL 213
>sp|P13175|RRP2_IAZI1 RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 28.9 bits (63), Expect = 3.8
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRTSIGQVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
>sp|P13172|RRP2_IARUD RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 28.9 bits (63), Expect = 3.8
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRTAVGQVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
>sp|P15659|RRP2_IAWIL RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 28.5 bits (62), Expect = 4.9
 Identities = 14/64 (21%), Positives = 32/64 (50%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + V ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEVGDMLLRSAIGHVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
>sp|P13178|RRP2_IATKM RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 28.5 bits (62), Expect = 4.9
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRTAIGQVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
>sp|P13174|RRP2_IAZH3 RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 28.5 bits (62), Expect = 4.9
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRTAIGQVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
>sp|P21427|RRP2_IAANN RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 28.5 bits (62), Expect = 4.9
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 50  NLKTRNIKIPVTRLKPLKEKWDDIVKILVKELNLQCKADPTKRPLTVYLRSPSNDEVKLQ 229
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRSAIGQVSRPMFLYVRTNGTSKIKMK 576

Query: 230 KGID 241
            G++
Sbjct: 577 WGME 580
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,044,852
Number of Sequences: 369166
Number of extensions: 528422
Number of successful extensions: 1473
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1472
length of database: 68,354,980
effective HSP length: 78
effective length of database: 53,945,650
effective search space used: 1672315150
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)