Planarian EST Database


Dr_sW_011_J15

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_011_J15
         (555 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O74777|MIS3_SCHPO  Ribosomal RNA assembly protein mis3          55   9e-08
sp|Q57885|Y443_METJA  Hypothetical protein MJ0443                  54   3e-07
sp|Q82570|RRP2_IAFOM  RNA-directed RNA polymerase subunit P2...    32   1.0  
sp|Q8K9P0|BIOA_BUCAP  Adenosylmethionine-8-amino-7-oxononano...    31   2.3  
sp|Q5PAG8|SYE2_ANAMM  Glutamyl-tRNA synthetase 2 (Glutamate-...    30   4.0  
sp|P26538|VL2_HPV58  Minor capsid protein L2                       30   5.2  
sp|O34204|TOP1_FERIS  DNA topoisomerase I (Omega-protein) (R...    30   5.2  
sp|P13175|RRP2_IAZI1  RNA-directed RNA polymerase subunit P2...    30   5.2  
sp|P13172|RRP2_IARUD  RNA-directed RNA polymerase subunit P2...    30   5.2  
sp|P38308|YB53_YEAST  Hypothetical 104.5 kDa protein in CDC4...    29   6.8  
>sp|O74777|MIS3_SCHPO Ribosomal RNA assembly protein mis3
          Length = 327

 Score = 55.5 bits (132), Expect = 9e-08
 Identities = 39/144 (27%), Positives = 66/144 (45%)
 Frame = +2

Query: 74  PVSRLKPLKEKWDDIVKILVKELYLQCKADPTKRPLTVYLRSPSNDEVKLQKGIDFLNAF 253
           P  R K L+E W  + + L K   + C  D  +  +TV     + D   +    D +   
Sbjct: 59  PKYREKYLREVWPHVTRALDK-FGITCVLDLVEGSMTVKTTRKTFDPYSILDARDLIKLL 117

Query: 254 SKGFDFIDCVSLIRMDGLYIRTMYIKDITRTIKGDHFTRAVGRMCGANGIMRKKIENATR 433
           ++   F   V +++ DG+    + I +I R    + F +   R+ G NG   K +E  T+
Sbjct: 118 ARSVPFPQAVKIMQ-DGVACDIIKIGNILRN--KERFVKRRQRLIGTNGQTLKALELLTQ 174

Query: 434 CRIVINETTVHIMGTYRGIDAAHR 505
           C I++  TTV +MG Y+G+    R
Sbjct: 175 CYILVQGTTVAVMGGYKGLKEVRR 198
>sp|Q57885|Y443_METJA Hypothetical protein MJ0443
          Length = 227

 Score = 53.9 bits (128), Expect = 3e-07
 Identities = 43/166 (25%), Positives = 76/166 (45%)
 Frame = +2

Query: 35  NIFDNLKTRNIKIPVSRLKPLKEKWDDIVKILVKELYLQCKADPTKRPLTVYLRSPSNDE 214
           NI  +     +KIP  R+  L  K  ++ K + KEL ++ + D     +T+Y      D 
Sbjct: 9   NIGQDKSIEILKIPKDRVGVLIGKKGNVKKTIEKELGVKLEID-ADGTVTIYGTDKQKDP 67

Query: 215 VKLQKGIDFLNAFSKGFDFIDCVSLIRMDGLYIRTMYIKDITRTIKGDHFTRAVGRMCGA 394
           + + K  D + A  +GF+    + L+  D   +  + I+D   +   +   R  GR+ G 
Sbjct: 68  LAVWKARDIVRAIGRGFNPEIALKLV-SDEYVLEVIDIEDYASS--DNSIRRLKGRVIGK 124

Query: 395 NGIMRKKIENATRCRIVINETTVHIMGTYRGIDAAHRKLGSLVRGA 532
            G  R+ IE+ T   + +   TV I+G +  +  A   +  L+RGA
Sbjct: 125 EGKSRRYIESLTGANVSVYGNTVAIVGEHEPVQIAKEAVEMLLRGA 170
>sp|Q82570|RRP2_IAFOM RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 32.0 bits (71), Expect = 1.0
 Identities = 14/64 (21%), Positives = 33/64 (51%)
 Frame = +2

Query: 47  NLKTRNIKIPVSRLKPLKEKWDDIVKILVKELYLQCKADPTKRPLTVYLRSPSNDEVKLQ 226
           N  +    +   RL+P   KW+    + + ++ L+    P  RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRSAIAPVSRPMFLYVRTNGTSKIKMK 576

Query: 227 KGID 238
            G++
Sbjct: 577 WGME 580
>sp|Q8K9P0|BIOA_BUCAP Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
           (7,8-diamino-pelargonic acid aminotransferase) (DAPA
           aminotransferase)
          Length = 432

 Score = 30.8 bits (68), Expect = 2.3
 Identities = 20/63 (31%), Positives = 31/63 (49%), Gaps = 6/63 (9%)
 Frame = +3

Query: 312 LERCILKISHALLKGITLPELLVECVGLMVLCEKKLKMQLVVES------*LMKQLFILW 473
           L++ I K+SH +  GIT P  ++ C  L+ L  KKL    + +S        +K L   W
Sbjct: 68  LKKQIRKMSHVMFGGITHPSAILLCRKLLKLTPKKLDCVFLSDSGSIAIEVAIKMLIQYW 127

Query: 474 EPI 482
           E +
Sbjct: 128 EAL 130
>sp|Q5PAG8|SYE2_ANAMM Glutamyl-tRNA synthetase 2 (Glutamate--tRNA ligase 2) (GluRS 2)
          Length = 466

 Score = 30.0 bits (66), Expect = 4.0
 Identities = 22/72 (30%), Positives = 36/72 (50%), Gaps = 7/72 (9%)
 Frame = +2

Query: 191 LRSPSNDEVKLQKGIDFLNAFSKGFDFIDCVSLIRMDG--LYIRTMYIKD----ITRTIK 352
           L+SP N E+K   G+      S   + ID + ++R +G   Y+  + + D    IT  I+
Sbjct: 141 LKSPENREIKFTDGV--YGEISVSSEQIDDMVILRSNGHPTYLLAVVVDDHDMCITHIIR 198

Query: 353 G-DHFTRAVGRM 385
           G DH T  + +M
Sbjct: 199 GSDHITNTIKQM 210
>sp|P26538|VL2_HPV58 Minor capsid protein L2
          Length = 472

 Score = 29.6 bits (65), Expect = 5.2
 Identities = 19/67 (28%), Positives = 34/67 (50%), Gaps = 5/67 (7%)
 Frame = -3

Query: 520 QRSKFSVSSINTSIGSHNMNSCFIN-YDSTTSCIFNF----FSHNTISPTHSTNSSGKVI 356
           Q+ +F + S+NTS+  +++N    + Y      I +F     SH + + T ++N S   I
Sbjct: 340 QQQQFELQSLNTSVSPYSINDGLYDIYADDADTIHDFQSPLHSHTSFATTRTSNVS---I 396

Query: 355 PFNSACD 335
           P N+  D
Sbjct: 397 PLNTGFD 403
>sp|O34204|TOP1_FERIS DNA topoisomerase I (Omega-protein) (Relaxing enzyme) (Untwisting
           enzyme) (Swivelase)
          Length = 699

 Score = 29.6 bits (65), Expect = 5.2
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = +2

Query: 2   IINFYLFLKVPNIFDNLKTRNIKIPVSRLKPLKEKWDDIVKILVKE 139
           ++N YL  + P+I D   T  ++  +  ++  K+ W ++VK  +KE
Sbjct: 533 VVNHYLEQRFPDIVDKGFTAEMEKELDEVENGKKDWKEVVKSFLKE 578
>sp|P13175|RRP2_IAZI1 RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 29.6 bits (65), Expect = 5.2
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 47  NLKTRNIKIPVSRLKPLKEKWDDIVKILVKELYLQCKADPTKRPLTVYLRSPSNDEVKLQ 226
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRTSIGQVSRPMFLYVRTNGTSKIKMK 576

Query: 227 KGID 238
            G++
Sbjct: 577 WGME 580
>sp|P13172|RRP2_IARUD RNA-directed RNA polymerase subunit P2 (Polymerase acidic protein)
           (PA)
          Length = 716

 Score = 29.6 bits (65), Expect = 5.2
 Identities = 13/64 (20%), Positives = 32/64 (50%)
 Frame = +2

Query: 47  NLKTRNIKIPVSRLKPLKEKWDDIVKILVKELYLQCKADPTKRPLTVYLRSPSNDEVKLQ 226
           N  +    +   RL+P   KW+    + + ++ L+       RP+ +Y+R+    ++K++
Sbjct: 519 NFVSMEFSLTDPRLEP--HKWEKYCVLEIGDMLLRTAVGQVSRPMFLYVRTNGTSKIKMK 576

Query: 227 KGID 238
            G++
Sbjct: 577 WGME 580
>sp|P38308|YB53_YEAST Hypothetical 104.5 kDa protein in CDC47-KTR3 intergenic region
          Length = 924

 Score = 29.3 bits (64), Expect = 6.8
 Identities = 16/31 (51%), Positives = 20/31 (64%)
 Frame = -3

Query: 520 QRSKFSVSSINTSIGSHNMNSCFINYDSTTS 428
           QRS  SV+SI TSI S   N+  ++  STTS
Sbjct: 390 QRSNSSVASITTSIMSSIYNTSHVSLSSTTS 420
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,139,067
Number of Sequences: 369166
Number of extensions: 1196287
Number of successful extensions: 3018
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3017
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3931403200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)