Planaria EST Database


DrC_01059

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01059
         (862 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9QZC7|PLEB2_MOUSE  Pleckstrin homology domain-containing...    69   2e-11
sp|Q96CS7|PLEB2_HUMAN  Pleckstrin homology domain-containing...    69   2e-11
sp|Q9QYE9|PLEB1_MOUSE  Pleckstrin homology domain-containing...    63   1e-09
sp|Q9WU68|PLEB1_RAT  Pleckstrin homology domain-containing p...    63   1e-09
sp|Q9UF11|PLEB1_HUMAN  Pleckstrin homology domain-containing...    61   4e-09
sp|Q9ST43|PH1_ARATH  Pleckstrin homology domain-containing p...    38   0.041
sp|P47197|AKT2_RAT  RAC-beta serine/threonine-protein kinase...    35   0.27 
sp|P31751|AKT2_HUMAN  RAC-beta serine/threonine-protein kina...    35   0.27 
sp|Q60823|AKT2_MOUSE  RAC-beta serine/threonine-protein kina...    35   0.27 
sp|P97694|CYH1_RAT  Cytohesin-1 (SEC7 homolog A) (msec7-1)         34   0.46 
>sp|Q9QZC7|PLEB2_MOUSE Pleckstrin homology domain-containing protein family B member 2
           (Evectin-2)
          Length = 221

 Score = 68.9 bits (167), Expect = 2e-11
 Identities = 33/102 (32%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
 Frame = +3

Query: 36  VKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF- 212
           VK+GWL R S +LK WK+ W  ++ DG + +   Q + S + K+++ ++C  +++  E  
Sbjct: 4   VKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQSIEDKVHMPVDCINIRTGHECR 63

Query: 213 ESKIPDGKTRPCLFGF-SNSESSYWFCAEDEDDAMVWRLVLE 335
           + + PDGK R CL         +   CAE  DD + W+  L+
Sbjct: 64  DIQPPDGKPRDCLLQIVCRDGKTISLCAESTDDCLAWKFTLQ 105
>sp|Q96CS7|PLEB2_HUMAN Pleckstrin homology domain-containing protein family B member 2
           (Evectin-2)
          Length = 222

 Score = 68.6 bits (166), Expect = 2e-11
 Identities = 30/102 (29%), Positives = 58/102 (56%), Gaps = 2/102 (1%)
 Frame = +3

Query: 36  VKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF- 212
           VK+GWL R S +LK WK+ W  ++ DG + +   Q + + + K+++ ++C  +++  E  
Sbjct: 4   VKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGQECR 63

Query: 213 ESKIPDGKTRPCLFGF-SNSESSYWFCAEDEDDAMVWRLVLE 335
           +++ PDGK++ C+         +   CAE  DD + W+  L+
Sbjct: 64  DTQPPDGKSKDCMLQIVCRDGKTISLCAESTDDCLAWKFTLQ 105
>sp|Q9QYE9|PLEB1_MOUSE Pleckstrin homology domain-containing protein family B member 1
           (Pleckstrin homology domain retinal protein 1) (PH
           domain containing protein in retina 1) (PHRET1)
          Length = 243

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 29/102 (28%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
 Frame = +3

Query: 33  IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF 212
           +V+ GWL+R S +L+ WKR W  +++DG++ +   +     + ++ +    +++K   E 
Sbjct: 22  LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGQEC 81

Query: 213 ES-KIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 332
           +  + P+G++R  L   +  E S    CAE  DDA+ W+  L
Sbjct: 82  QDVQPPEGRSRDGLLTVNLREGSRLHLCAETRDDAIAWKTAL 123
>sp|Q9WU68|PLEB1_RAT Pleckstrin homology domain-containing protein family B member 1
           (Evectin-1)
          Length = 223

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 29/102 (28%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
 Frame = +3

Query: 33  IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF 212
           +V+ GWL+R S +L+ WKR W  +++DG++ +   +     + ++ +    +++K   E 
Sbjct: 3   LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGQEC 62

Query: 213 ES-KIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 332
           +  + P+G++R  L   +  E S    CAE  DDA+ W+  L
Sbjct: 63  QDVQPPEGRSRDGLLTVNLREGSRLHLCAETRDDAIAWKTAL 104
>sp|Q9UF11|PLEB1_HUMAN Pleckstrin homology domain-containing protein family B member 1
           (Pleckstrin homology domain retinal protein 1) (PH
           domain containing protein in retina 1) (PHRET1)
          Length = 243

 Score = 61.2 bits (147), Expect = 4e-09
 Identities = 28/102 (27%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
 Frame = +3

Query: 33  IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDE- 209
           +V+ GWL+R S +L+ WKR W  +++DG++ +   +     + ++ +    +++K   E 
Sbjct: 22  LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVLIHFNVRDIKIGPEC 81

Query: 210 FESKIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 332
            + + P+G++R  L   +  E      CAE +DDA+ W+  L
Sbjct: 82  HDVQPPEGRSRDGLLTVNLREGGRLHLCAETKDDALAWKTAL 123
>sp|Q9ST43|PH1_ARATH Pleckstrin homology domain-containing protein 1 (AtPH1)
          Length = 145

 Score = 37.7 bits (86), Expect = 0.041
 Identities = 23/101 (22%), Positives = 47/101 (46%), Gaps = 4/101 (3%)
 Frame = +3

Query: 30  NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQD----KMSPDKKINLILECKEMK 197
           N  ++GWL +  D +K W+R W ++   G + + K Q     + S  + +  + +C  +K
Sbjct: 26  NPERSGWLTKQGDYIKTWRRRWFVL-KRGKLLWFKDQAAAGIRGSTPRGVISVGDCLTVK 84

Query: 198 SYDEFESKIPDGKTRPCLFGFSNSESSYWFCAEDEDDAMVW 320
             ++  +K       P  F  S+   + +F A++E +   W
Sbjct: 85  GAEDVVNK-------PFAFELSSGSYTMFFIADNEKEKEEW 118
>sp|P47197|AKT2_RAT RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
           kinase Akt-2) (Protein kinase B, beta) (PKB beta)
          Length = 481

 Score = 35.0 bits (79), Expect = 0.27
 Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
 Frame = +3

Query: 30  NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 197
           +++K GWL++  + +K W+  + ++  DGS    K +    D+  P      + EC+ MK
Sbjct: 5   SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64

Query: 198 S 200
           +
Sbjct: 65  T 65
>sp|P31751|AKT2_HUMAN RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
           kinase Akt-2) (Protein kinase B, beta) (PKB beta)
          Length = 481

 Score = 35.0 bits (79), Expect = 0.27
 Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
 Frame = +3

Query: 30  NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 197
           +++K GWL++  + +K W+  + ++  DGS    K +    D+  P      + EC+ MK
Sbjct: 5   SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64

Query: 198 S 200
           +
Sbjct: 65  T 65
>sp|Q60823|AKT2_MOUSE RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
           kinase Akt-2) (Protein kinase B, beta) (PKB beta)
          Length = 481

 Score = 35.0 bits (79), Expect = 0.27
 Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
 Frame = +3

Query: 30  NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 197
           +++K GWL++  + +K W+  + ++  DGS    K +    D+  P      + EC+ MK
Sbjct: 5   SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64

Query: 198 S 200
           +
Sbjct: 65  T 65
>sp|P97694|CYH1_RAT Cytohesin-1 (SEC7 homolog A) (msec7-1)
          Length = 398

 Score = 34.3 bits (77), Expect = 0.46
 Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 5/75 (6%)
 Frame = +3

Query: 27  FNIVKAGWLYRLSDV-LKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINL----ILECKE 191
           FN  + GWL +L    +K WKR W I+  D  + + +      P   I L    I E ++
Sbjct: 259 FNPDREGWLLKLGGGRVKTWKRRWFIL-TDNCLYYFEYTTDKEPRGIIPLENLSIREVED 317

Query: 192 MKSYDEFESKIPDGK 236
            K  + FE  IPD K
Sbjct: 318 SKKPNCFELYIPDNK 332
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,201,528
Number of Sequences: 369166
Number of extensions: 1621233
Number of successful extensions: 3461
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3388
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3455
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01059

  1. Dr_sW_028_E05
  2. Dr_sW_026_K13
  3. Dr_sW_028_H01