Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_K13
(859 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9QZC7|PLEB2_MOUSE Pleckstrin homology domain-containing... 69 2e-11
sp|Q96CS7|PLEB2_HUMAN Pleckstrin homology domain-containing... 69 2e-11
sp|Q9QYE9|PLEB1_MOUSE Pleckstrin homology domain-containing... 63 1e-09
sp|Q9WU68|PLEB1_RAT Pleckstrin homology domain-containing p... 63 1e-09
sp|Q9UF11|PLEB1_HUMAN Pleckstrin homology domain-containing... 61 3e-09
sp|Q9ST43|PH1_ARATH Pleckstrin homology domain-containing p... 38 0.041
sp|P47197|AKT2_RAT RAC-beta serine/threonine-protein kinase... 35 0.27
sp|P31751|AKT2_HUMAN RAC-beta serine/threonine-protein kina... 35 0.27
sp|Q60823|AKT2_MOUSE RAC-beta serine/threonine-protein kina... 35 0.27
sp|P97694|CYH1_RAT Cytohesin-1 (SEC7 homolog A) (msec7-1) 34 0.46
>sp|Q9QZC7|PLEB2_MOUSE Pleckstrin homology domain-containing protein family B member 2
(Evectin-2)
Length = 221
Score = 68.9 bits (167), Expect = 2e-11
Identities = 33/102 (32%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +3
Query: 36 VKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF- 212
VK+GWL R S +LK WK+ W ++ DG + + Q + S + K+++ ++C +++ E
Sbjct: 4 VKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQSIEDKVHMPVDCINIRTGHECR 63
Query: 213 ESKIPDGKTRPCLFGF-SNSESSYWFCAEDEDDAMVWRLVLE 335
+ + PDGK R CL + CAE DD + W+ L+
Sbjct: 64 DIQPPDGKPRDCLLQIVCRDGKTISLCAESTDDCLAWKFTLQ 105
>sp|Q96CS7|PLEB2_HUMAN Pleckstrin homology domain-containing protein family B member 2
(Evectin-2)
Length = 222
Score = 68.6 bits (166), Expect = 2e-11
Identities = 30/102 (29%), Positives = 58/102 (56%), Gaps = 2/102 (1%)
Frame = +3
Query: 36 VKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF- 212
VK+GWL R S +LK WK+ W ++ DG + + Q + + + K+++ ++C +++ E
Sbjct: 4 VKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGQECR 63
Query: 213 ESKIPDGKTRPCLFGF-SNSESSYWFCAEDEDDAMVWRLVLE 335
+++ PDGK++ C+ + CAE DD + W+ L+
Sbjct: 64 DTQPPDGKSKDCMLQIVCRDGKTISLCAESTDDCLAWKFTLQ 105
>sp|Q9QYE9|PLEB1_MOUSE Pleckstrin homology domain-containing protein family B member 1
(Pleckstrin homology domain retinal protein 1) (PH
domain containing protein in retina 1) (PHRET1)
Length = 243
Score = 62.8 bits (151), Expect = 1e-09
Identities = 29/102 (28%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +3
Query: 33 IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF 212
+V+ GWL+R S +L+ WKR W +++DG++ + + + ++ + +++K E
Sbjct: 22 LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGQEC 81
Query: 213 ES-KIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 332
+ + P+G++R L + E S CAE DDA+ W+ L
Sbjct: 82 QDVQPPEGRSRDGLLTVNLREGSRLHLCAETRDDAIAWKTAL 123
>sp|Q9WU68|PLEB1_RAT Pleckstrin homology domain-containing protein family B member 1
(Evectin-1)
Length = 223
Score = 62.8 bits (151), Expect = 1e-09
Identities = 29/102 (28%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +3
Query: 33 IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF 212
+V+ GWL+R S +L+ WKR W +++DG++ + + + ++ + +++K E
Sbjct: 3 LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGQEC 62
Query: 213 ES-KIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 332
+ + P+G++R L + E S CAE DDA+ W+ L
Sbjct: 63 QDVQPPEGRSRDGLLTVNLREGSRLHLCAETRDDAIAWKTAL 104
>sp|Q9UF11|PLEB1_HUMAN Pleckstrin homology domain-containing protein family B member 1
(Pleckstrin homology domain retinal protein 1) (PH
domain containing protein in retina 1) (PHRET1)
Length = 243
Score = 61.2 bits (147), Expect = 3e-09
Identities = 28/102 (27%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +3
Query: 33 IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDE- 209
+V+ GWL+R S +L+ WKR W +++DG++ + + + ++ + +++K E
Sbjct: 22 LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVLIHFNVRDIKIGPEC 81
Query: 210 FESKIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 332
+ + P+G++R L + E CAE +DDA+ W+ L
Sbjct: 82 HDVQPPEGRSRDGLLTVNLREGGRLHLCAETKDDALAWKTAL 123
>sp|Q9ST43|PH1_ARATH Pleckstrin homology domain-containing protein 1 (AtPH1)
Length = 145
Score = 37.7 bits (86), Expect = 0.041
Identities = 23/101 (22%), Positives = 47/101 (46%), Gaps = 4/101 (3%)
Frame = +3
Query: 30 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQD----KMSPDKKINLILECKEMK 197
N ++GWL + D +K W+R W ++ G + + K Q + S + + + +C +K
Sbjct: 26 NPERSGWLTKQGDYIKTWRRRWFVL-KRGKLLWFKDQAAAGIRGSTPRGVISVGDCLTVK 84
Query: 198 SYDEFESKIPDGKTRPCLFGFSNSESSYWFCAEDEDDAMVW 320
++ +K P F S+ + +F A++E + W
Sbjct: 85 GAEDVVNK-------PFAFELSSGSYTMFFIADNEKEKEEW 118
>sp|P47197|AKT2_RAT RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
kinase Akt-2) (Protein kinase B, beta) (PKB beta)
Length = 481
Score = 35.0 bits (79), Expect = 0.27
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
Frame = +3
Query: 30 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 197
+++K GWL++ + +K W+ + ++ DGS K + D+ P + EC+ MK
Sbjct: 5 SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64
Query: 198 S 200
+
Sbjct: 65 T 65
>sp|P31751|AKT2_HUMAN RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
kinase Akt-2) (Protein kinase B, beta) (PKB beta)
Length = 481
Score = 35.0 bits (79), Expect = 0.27
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
Frame = +3
Query: 30 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 197
+++K GWL++ + +K W+ + ++ DGS K + D+ P + EC+ MK
Sbjct: 5 SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64
Query: 198 S 200
+
Sbjct: 65 T 65
>sp|Q60823|AKT2_MOUSE RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
kinase Akt-2) (Protein kinase B, beta) (PKB beta)
Length = 481
Score = 35.0 bits (79), Expect = 0.27
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
Frame = +3
Query: 30 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 197
+++K GWL++ + +K W+ + ++ DGS K + D+ P + EC+ MK
Sbjct: 5 SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64
Query: 198 S 200
+
Sbjct: 65 T 65
>sp|P97694|CYH1_RAT Cytohesin-1 (SEC7 homolog A) (msec7-1)
Length = 398
Score = 34.3 bits (77), Expect = 0.46
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 5/75 (6%)
Frame = +3
Query: 27 FNIVKAGWLYRLSDV-LKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINL----ILECKE 191
FN + GWL +L +K WKR W I+ D + + + P I L I E ++
Sbjct: 259 FNPDREGWLLKLGGGRVKTWKRRWFIL-TDNCLYYFEYTTDKEPRGIIPLENLSIREVED 317
Query: 192 MKSYDEFESKIPDGK 236
K + FE IPD K
Sbjct: 318 SKKPNCFELYIPDNK 332
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,351,653
Number of Sequences: 369166
Number of extensions: 1601117
Number of successful extensions: 3498
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3427
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3492
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)