Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_E05
(528 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9QZC7|PLEB2_MOUSE Pleckstrin homology domain-containing... 69 7e-12
sp|Q96CS7|PLEB2_HUMAN Pleckstrin homology domain-containing... 69 9e-12
sp|Q9QYE9|PLEB1_MOUSE Pleckstrin homology domain-containing... 63 5e-10
sp|Q9WU68|PLEB1_RAT Pleckstrin homology domain-containing p... 63 5e-10
sp|Q9UF11|PLEB1_HUMAN Pleckstrin homology domain-containing... 61 1e-09
sp|Q9ST43|PH1_ARATH Pleckstrin homology domain-containing p... 38 0.017
sp|P47197|AKT2_RAT RAC-beta serine/threonine-protein kinase... 35 0.11
sp|P31751|AKT2_HUMAN RAC-beta serine/threonine-protein kina... 35 0.11
sp|Q60823|AKT2_MOUSE RAC-beta serine/threonine-protein kina... 35 0.11
sp|P97694|CYH1_RAT Cytohesin-1 (SEC7 homolog A) (msec7-1) 34 0.19
>sp|Q9QZC7|PLEB2_MOUSE Pleckstrin homology domain-containing protein family B member 2
(Evectin-2)
Length = 221
Score = 68.9 bits (167), Expect = 7e-12
Identities = 33/102 (32%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +2
Query: 32 VKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF- 208
VK+GWL R S +LK WK+ W ++ DG + + Q + S + K+++ ++C +++ E
Sbjct: 4 VKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQSIEDKVHMPVDCINIRTGHECR 63
Query: 209 ESKIPDGKTRPCLFGF-SNSESSYWFCAEDEDDAMVWRLVLE 331
+ + PDGK R CL + CAE DD + W+ L+
Sbjct: 64 DIQPPDGKPRDCLLQIVCRDGKTISLCAESTDDCLAWKFTLQ 105
>sp|Q96CS7|PLEB2_HUMAN Pleckstrin homology domain-containing protein family B member 2
(Evectin-2)
Length = 222
Score = 68.6 bits (166), Expect = 9e-12
Identities = 30/102 (29%), Positives = 58/102 (56%), Gaps = 2/102 (1%)
Frame = +2
Query: 32 VKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF- 208
VK+GWL R S +LK WK+ W ++ DG + + Q + + + K+++ ++C +++ E
Sbjct: 4 VKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGQECR 63
Query: 209 ESKIPDGKTRPCLFGF-SNSESSYWFCAEDEDDAMVWRLVLE 331
+++ PDGK++ C+ + CAE DD + W+ L+
Sbjct: 64 DTQPPDGKSKDCMLQIVCRDGKTISLCAESTDDCLAWKFTLQ 105
>sp|Q9QYE9|PLEB1_MOUSE Pleckstrin homology domain-containing protein family B member 1
(Pleckstrin homology domain retinal protein 1) (PH
domain containing protein in retina 1) (PHRET1)
Length = 243
Score = 62.8 bits (151), Expect = 5e-10
Identities = 29/102 (28%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +2
Query: 29 IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF 208
+V+ GWL+R S +L+ WKR W +++DG++ + + + ++ + +++K E
Sbjct: 22 LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGQEC 81
Query: 209 ES-KIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 328
+ + P+G++R L + E S CAE DDA+ W+ L
Sbjct: 82 QDVQPPEGRSRDGLLTVNLREGSRLHLCAETRDDAIAWKTAL 123
>sp|Q9WU68|PLEB1_RAT Pleckstrin homology domain-containing protein family B member 1
(Evectin-1)
Length = 223
Score = 62.8 bits (151), Expect = 5e-10
Identities = 29/102 (28%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +2
Query: 29 IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDEF 208
+V+ GWL+R S +L+ WKR W +++DG++ + + + ++ + +++K E
Sbjct: 3 LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGQEC 62
Query: 209 ES-KIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 328
+ + P+G++R L + E S CAE DDA+ W+ L
Sbjct: 63 QDVQPPEGRSRDGLLTVNLREGSRLHLCAETRDDAIAWKTAL 104
>sp|Q9UF11|PLEB1_HUMAN Pleckstrin homology domain-containing protein family B member 1
(Pleckstrin homology domain retinal protein 1) (PH
domain containing protein in retina 1) (PHRET1)
Length = 243
Score = 61.2 bits (147), Expect = 1e-09
Identities = 28/102 (27%), Positives = 56/102 (54%), Gaps = 2/102 (1%)
Frame = +2
Query: 29 IVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINLILECKEMKSYDE- 205
+V+ GWL+R S +L+ WKR W +++DG++ + + + ++ + +++K E
Sbjct: 22 LVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVLIHFNVRDIKIGPEC 81
Query: 206 FESKIPDGKTRPCLFGFSNSESS-YWFCAEDEDDAMVWRLVL 328
+ + P+G++R L + E CAE +DDA+ W+ L
Sbjct: 82 HDVQPPEGRSRDGLLTVNLREGGRLHLCAETKDDALAWKTAL 123
>sp|Q9ST43|PH1_ARATH Pleckstrin homology domain-containing protein 1 (AtPH1)
Length = 145
Score = 37.7 bits (86), Expect = 0.017
Identities = 23/101 (22%), Positives = 47/101 (46%), Gaps = 4/101 (3%)
Frame = +2
Query: 26 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQD----KMSPDKKINLILECKEMK 193
N ++GWL + D +K W+R W ++ G + + K Q + S + + + +C +K
Sbjct: 26 NPERSGWLTKQGDYIKTWRRRWFVL-KRGKLLWFKDQAAAGIRGSTPRGVISVGDCLTVK 84
Query: 194 SYDEFESKIPDGKTRPCLFGFSNSESSYWFCAEDEDDAMVW 316
++ +K P F S+ + +F A++E + W
Sbjct: 85 GAEDVVNK-------PFAFELSSGSYTMFFIADNEKEKEEW 118
>sp|P47197|AKT2_RAT RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
kinase Akt-2) (Protein kinase B, beta) (PKB beta)
Length = 481
Score = 35.0 bits (79), Expect = 0.11
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
Frame = +2
Query: 26 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 193
+++K GWL++ + +K W+ + ++ DGS K + D+ P + EC+ MK
Sbjct: 5 SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64
Query: 194 S 196
+
Sbjct: 65 T 65
>sp|P31751|AKT2_HUMAN RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
kinase Akt-2) (Protein kinase B, beta) (PKB beta)
Length = 481
Score = 35.0 bits (79), Expect = 0.11
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
Frame = +2
Query: 26 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 193
+++K GWL++ + +K W+ + ++ DGS K + D+ P + EC+ MK
Sbjct: 5 SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64
Query: 194 S 196
+
Sbjct: 65 T 65
>sp|Q60823|AKT2_MOUSE RAC-beta serine/threonine-protein kinase (RAC-PK-beta) (Protein
kinase Akt-2) (Protein kinase B, beta) (PKB beta)
Length = 481
Score = 35.0 bits (79), Expect = 0.11
Identities = 16/61 (26%), Positives = 32/61 (52%), Gaps = 4/61 (6%)
Frame = +2
Query: 26 NIVKAGWLYRLSDVLKVWKREWTIIYVDGSMSFLKSQ----DKMSPDKKINLILECKEMK 193
+++K GWL++ + +K W+ + ++ DGS K + D+ P + EC+ MK
Sbjct: 5 SVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMK 64
Query: 194 S 196
+
Sbjct: 65 T 65
>sp|P97694|CYH1_RAT Cytohesin-1 (SEC7 homolog A) (msec7-1)
Length = 398
Score = 34.3 bits (77), Expect = 0.19
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 5/75 (6%)
Frame = +2
Query: 23 FNIVKAGWLYRLSDV-LKVWKREWTIIYVDGSMSFLKSQDKMSPDKKINL----ILECKE 187
FN + GWL +L +K WKR W I+ D + + + P I L I E ++
Sbjct: 259 FNPDREGWLLKLGGGRVKTWKRRWFIL-TDNCLYYFEYTTDKEPRGIIPLENLSIREVED 317
Query: 188 MKSYDEFESKIPDGK 232
K + FE IPD K
Sbjct: 318 SKKPNCFELYIPDNK 332
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,939,127
Number of Sequences: 369166
Number of extensions: 998198
Number of successful extensions: 2537
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2531
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3551563800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)