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Planaria EST Database

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01029
         (1096 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q12236|KOK0_YEAST  Probable serine/threonine-protein kina...   129   1e-29
sp|Q99759|M3K3_HUMAN  Mitogen-activated protein kinase kinas...   128   3e-29
sp|Q61084|M3K3_MOUSE  Mitogen-activated protein kinase kinas...   127   6e-29
sp|Q13233|M3K1_HUMAN  Mitogen-activated protein kinase kinas...   125   2e-28
sp|P50527|SHK1_SCHPO  Serine/threonine-protein kinase pak1/shk1   125   2e-28
sp|P53349|M3K1_MOUSE  Mitogen-activated protein kinase kinas...   125   2e-28
sp|P27636|CDC15_YEAST  Cell division control protein 15           125   3e-28
sp|Q99683|M3K5_HUMAN  Mitogen-activated protein kinase kinas...   124   6e-28
sp|Q9Y2U5|M3K2_HUMAN  Mitogen-activated protein kinase kinas...   124   6e-28
sp|Q62925|M3K1_RAT  Mitogen-activated protein kinase kinase ...   123   8e-28

>sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kinase YOL100W
          Length = 1081

 Score =  129 bits (324), Expect = 1e-29
 Identities = 71/239 (29%), Positives = 118/239 (49%), Gaps = 5/239 (2%)
 Frame = +3

Query: 282 ILMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLGFFKDELYFYIAMEHMERGSLHFY 461
           +L K Y + ++  K V  E  +  KL++ P ++ L   F+DE   Y  +E+   G     
Sbjct: 209 VLNKEYLIRQKKVKYVSIEKTALQKLNNSPSVVRLFSTFQDESSLYFLLEYAPNGDFLSL 268

Query: 462 IKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYL 641
           +K   S++E   R ++ QI+  + YLH N +IH D+K  NILLD  + IK++ F  +K L
Sbjct: 269 MKKYGSLDETCARYYAAQIIDAIDYLHSNGIIHRDIKPENILLDGEMKIKLTDFGTAKLL 328

Query: 642 AGANSTVMSEGF-----MQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVELVTGRQP 806
              N++V    +      +S  GT  +++PE+L D     + D+W+FG +L +++ G+ P
Sbjct: 329 NPTNNSVSKPEYDLSTRSKSFVGTAEYVSPELLNDSFTDYRCDIWAFGCILFQMIAGKPP 388

Query: 807 FYGLESINIILKIADEQTIDYVpreGMSDHLRDFIAKCLTGKRKDRWNADQLLEHDFLK 983
           F   ++ N  L       + Y    G    +RD + K L      R    Q+ EH F K
Sbjct: 389 F---KATNEYLTFQKVMKVQYAFTPGFPLIIRDLVKKILVKNLDRRLTISQIKEHHFFK 444

>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
            kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  128 bits (322), Expect = 3e-29
 Identities = 77/206 (37%), Positives = 121/206 (58%), Gaps = 2/206 (0%)
 Frame = +3

Query: 366  HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 539
            H RI+   G  +D  E    I ME+M  GS+   +K   ++ E + RK++ QIL+G+SYL
Sbjct: 421  HERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480

Query: 540  HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 719
            H N ++H D+K +NIL D+  ++K+  F  SK L    +  MS   M+S  GT  +M+PE
Sbjct: 481  HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGMRSVTGTPYWMSPE 537

Query: 720  VLIDQKITRKSDVWSFGAVLVELVTGRQPFYGLESINIILKIADEQTIDYVpreGMSDHL 899
            V+  +   RK+DVWS G  +VE++T + P+   E++  I KIA + T   +P   +S+H 
Sbjct: 538  VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSH-ISEHG 596

Query: 900  RDFIAKCLTGKRKDRWNADQLLEHDF 977
            RDF+ +     R+ R +A++LL H F
Sbjct: 597  RDFLRRIFVEARQ-RPSAEELLTHHF 621

>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
            kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  127 bits (319), Expect = 6e-29
 Identities = 76/206 (36%), Positives = 121/206 (58%), Gaps = 2/206 (0%)
 Frame = +3

Query: 366  HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 539
            H RI+   G  +D  E    I ME+M  GS+   +K   ++ E + RK++ QIL+G+SYL
Sbjct: 421  HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480

Query: 540  HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 719
            H N ++H D+K +NIL D+  ++K+  F  SK L    +  MS   ++S  GT  +M+PE
Sbjct: 481  HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPE 537

Query: 720  VLIDQKITRKSDVWSFGAVLVELVTGRQPFYGLESINIILKIADEQTIDYVpreGMSDHL 899
            V+  +   RK+DVWS G  +VE++T + P+   E++  I KIA + T   +P   +S+H 
Sbjct: 538  VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSH-ISEHG 596

Query: 900  RDFIAKCLTGKRKDRWNADQLLEHDF 977
            RDF+ +     R+ R +A++LL H F
Sbjct: 597  RDFLRRIFVEARQ-RPSAEELLTHHF 621

>sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
            kinase 1) (MEK kinase 1) (MEKK 1)
          Length = 1495

 Score =  125 bits (315), Expect = 2e-28
 Identities = 83/260 (31%), Positives = 138/260 (53%), Gaps = 10/260 (3%)
 Frame = +3

Query: 222  VAVGQFSQVYTARIVGNETVILMKRYTVA------EQSYKPVLRELVSFAKLDDHPRIIN 383
            + +G FS  Y A+ VG  T++ +K+ T        ++     LRE +      +HP II 
Sbjct: 1232 IGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIR 1291

Query: 384  LLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHC 563
            +LG   ++  + + +E M  GS+   +    +  E ++  ++ Q+L+GLSYLH+N++IH 
Sbjct: 1292 MLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHR 1351

Query: 564  DLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKI 740
            D+K +N+L+D+    ++I+ F  +  LA +  T   E F     GT  FMAPEVL  Q+ 
Sbjct: 1352 DVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEVLRGQQY 1409

Query: 741  TRKSDVWSFGAVLVELVTGRQPFYGLESIN---IILKIADEQTIDYVpreGMSDHLRDFI 911
             R  DVWS G  ++E+   + P+   +  N   +I KIA   T   +P   +S  LRD  
Sbjct: 1410 GRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSH-LSPGLRDVA 1468

Query: 912  AKCLTGKRKDRWNADQLLEH 971
             +CL  + +DR  + +LL+H
Sbjct: 1469 LRCLELQPQDRPPSRELLKH 1488

>sp|P50527|SHK1_SCHPO Serine/threonine-protein kinase pak1/shk1
          Length = 658

 Score =  125 bits (315), Expect = 2e-28
 Identities = 88/271 (32%), Positives = 135/271 (49%), Gaps = 2/271 (0%)
 Frame = +3

Query: 177  CEIKNFGELIEGNNHVAVGQFSQVYTARIVGNETVILMKRYTVAEQSYKPVLRELVSFAK 356
            C  KN   L      +  G    VY+AR VG    + +K+  + +Q  K  +   +   K
Sbjct: 377  CNPKNPTLLYRNFVKIGQGASGDVYSARQVGTNLSVAIKKMNINQQPKKEFIVNEILVMK 436

Query: 357  LDDHPRIINLLG--FFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGL 530
               H  I+N +   F+K EL+  + ME+M  GSL   + NN+ ++E  I     + L+GL
Sbjct: 437  SHHHKNIVNFIDTFFYKSELW--MVMEYMRGGSLTEVVTNNT-LSEGQIAAICKETLEGL 493

Query: 531  SYLHKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFM 710
             +LH+N ++H D+KS NILL    DIK++ F    + A  +S +     M    GT  +M
Sbjct: 494  QHLHENGIVHRDIKSDNILLSLQGDIKLTDFG---FCAQIDSNMTKRTTMV---GTPYWM 547

Query: 711  APEVLIDQKITRKSDVWSFGAVLVELVTGRQPFYGLESINIILKIADEQTIDYVpreGMS 890
            APEV+  ++   K DVWS G + +E+V G  P+     +  +  IA   T      E +S
Sbjct: 548  APEVVTRKEYGFKVDVWSLGIMAIEMVEGEPPYLNENPLRALYLIATIGTPKISRPELLS 607

Query: 891  DHLRDFIAKCLTGKRKDRWNADQLLEHDFLK 983
                DF++K LT   K R ++ +LL H FLK
Sbjct: 608  SVFHDFLSKSLTVNPKQRPSSGELLRHPFLK 638

>sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
            kinase 1) (MEK kinase 1) (MEKK 1)
          Length = 1493

 Score =  125 bits (314), Expect = 2e-28
 Identities = 83/260 (31%), Positives = 138/260 (53%), Gaps = 10/260 (3%)
 Frame = +3

Query: 222  VAVGQFSQVYTARIVGNETVILMKRYTVA------EQSYKPVLRELVSFAKLDDHPRIIN 383
            + +G FS  Y A+ VG  T++ +K+ T        ++     LRE +      +HP II 
Sbjct: 1230 IGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIR 1289

Query: 384  LLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHC 563
            +LG   ++  + + +E M  GS+   +    +  E ++  ++ Q+L+GLSYLH+N++IH 
Sbjct: 1290 MLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHR 1349

Query: 564  DLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKI 740
            D+K +N+L+D+    ++I+ F  +  LA +  T   E F     GT  FMAPEVL  Q+ 
Sbjct: 1350 DVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEVLRGQQY 1407

Query: 741  TRKSDVWSFGAVLVELVTGRQPFYGLESIN---IILKIADEQTIDYVpreGMSDHLRDFI 911
             R  DVWS G  ++E+   + P+   +  N   +I KIA   T   +P   +S  LRD  
Sbjct: 1408 GRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSH-LSPGLRDVA 1466

Query: 912  AKCLTGKRKDRWNADQLLEH 971
             +CL  + +DR  + +LL+H
Sbjct: 1467 VRCLELQPQDRPPSRELLKH 1486

>sp|P27636|CDC15_YEAST Cell division control protein 15
          Length = 974

 Score =  125 bits (313), Expect = 3e-28
 Identities = 77/248 (31%), Positives = 125/248 (50%), Gaps = 1/248 (0%)
 Frame = +3

Query: 231 GQFSQVYTARIVGNETVILMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLGFFKDEL 410
           G +  VY A     + V+ +K            +   +S  K  +H  I+   GF +   
Sbjct: 34  GSYGVVYKAINKHTDQVVAIKEVVYENDEELNDIMAEISLLKNLNHNNIVKYHGFIRKSY 93

Query: 411 YFYIAMEHMERGSLHFYIKNNSS-INEDLIRKWSLQILQGLSYLHKNRVIHCDLKSSNIL 587
             YI +E+   GSL   I  +S+ ++E+  + +  Q L GL YLH   VIH D+K++NIL
Sbjct: 94  ELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANIL 153

Query: 588 LDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSF 767
           L  +  +K++ F +S  +  +  T+          GT N+MAPE+L ++  +  SD+WS 
Sbjct: 154 LSADNTVKLADFGVSTIVNSSALTLA---------GTLNWMAPEILGNRGASTLSDIWSL 204

Query: 768 GAVLVELVTGRQPFYGLESINIILKIADEQTIDYVpreGMSDHLRDFIAKCLTGKRKDRW 947
           GA +VE++T   P++ L   NI   + ++    Y P    S+ L+DF++KC       R 
Sbjct: 205 GATVVEMLTKNPPYHNLTDANIYYAVEND---TYYPPSSFSEPLKDFLSKCFVKNMYKRP 261

Query: 948 NADQLLEH 971
            ADQLL+H
Sbjct: 262 TADQLLKH 269

>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
            kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
            signal-regulating kinase 1) (ASK-1)
          Length = 1374

 Score =  124 bits (310), Expect = 6e-28
 Identities = 86/271 (31%), Positives = 134/271 (49%), Gaps = 9/271 (3%)
 Frame = +3

Query: 198  ELIEGNNHVAVGQ--FSQVYTARIVGNETVILMKRYTVAEQSYKPVLRELVSFAKLDDHP 371
            E  E  + V +G+  +  VY  R + N+  I +K     +  Y   L E ++  K   H 
Sbjct: 676  EYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHK 735

Query: 372  RIINLLGFFKDELYFYIAMEHMERGSLHFYIKNN---SSINEDLIRKWSLQILQGLSYLH 542
             I+  LG F +  +  I ME +  GSL   +++       NE  I  ++ QIL+GL YLH
Sbjct: 736  NIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLH 795

Query: 543  KNRVIHCDLKSSNILLDNNLDI-KISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 719
             N+++H D+K  N+L++    + KIS F  SK LAG N         ++  GT  +MAPE
Sbjct: 796  DNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCT------ETFTGTLQYMAPE 849

Query: 720  VLI--DQKITRKSDVWSFGAVLVELVTGRQPFYGL-ESINIILKIADEQTIDYVpreGMS 890
            ++    +   + +D+WS G  ++E+ TG+ PFY L E    + K+   +    +P E MS
Sbjct: 850  IIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIP-ESMS 908

Query: 891  DHLRDFIAKCLTGKRKDRWNADQLLEHDFLK 983
               + FI KC       R  A+ LL  +FLK
Sbjct: 909  AEAKAFILKCFEPDPDKRACANDLLVDEFLK 939

>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
            kinase 2) (MEK kinase 2) (MEKK 2)
          Length = 618

 Score =  124 bits (310), Expect = 6e-28
 Identities = 75/209 (35%), Positives = 121/209 (57%), Gaps = 2/209 (0%)
 Frame = +3

Query: 366  HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 539
            H RI+   G  +D  E    I ME+M  GS+   +K   ++ E+  RK++ QIL+G+ YL
Sbjct: 414  HERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYL 473

Query: 540  HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 719
            H N ++H D+K +NIL D+  ++K+  F  SK L    +  +S   M+S  GT  +M+PE
Sbjct: 474  HSNMILHRDIKGANILRDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPE 530

Query: 720  VLIDQKITRKSDVWSFGAVLVELVTGRQPFYGLESINIILKIADEQTIDYVpreGMSDHL 899
            V+  Q   RK+D+WS    +VE++T + P+   E++  I KIA + T   +P   +SD+ 
Sbjct: 531  VISGQGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPH-VSDYT 589

Query: 900  RDFIAKCLTGKRKDRWNADQLLEHDFLKF 986
            RDF+ +    + K R +AD+LL H F+ +
Sbjct: 590  RDFLKRIFV-EAKLRPSADELLRHMFVHY 617

>sp|Q62925|M3K1_RAT Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
            kinase 1) (MEK kinase 1) (MEKK 1)
          Length = 1493

 Score =  123 bits (309), Expect = 8e-28
 Identities = 82/260 (31%), Positives = 137/260 (52%), Gaps = 10/260 (3%)
 Frame = +3

Query: 222  VAVGQFSQVYTARIVGNETVILMKRYTVA------EQSYKPVLRELVSFAKLDDHPRIIN 383
            + +G FS  Y A+ VG  T++ +K+ T        ++     LRE +      +HP II 
Sbjct: 1230 IGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIR 1289

Query: 384  LLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHC 563
            +LG   ++  + + +E M   S+   +    +  E ++  ++ Q+L+GLSYLH+N++IH 
Sbjct: 1290 MLGATCEKSNYNLFIEWMAGASVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHR 1349

Query: 564  DLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKI 740
            D+K +N+L+D+    ++I+ F  +  LA +  T   E F     GT  FMAPEVL  Q+ 
Sbjct: 1350 DVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEVLRGQQY 1407

Query: 741  TRKSDVWSFGAVLVELVTGRQPFYGLESIN---IILKIADEQTIDYVpreGMSDHLRDFI 911
             R  DVWS G  ++E+   + P+   +  N   +I KIA   T   +P   +S  LRD  
Sbjct: 1408 GRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSH-LSPGLRDVA 1466

Query: 912  AKCLTGKRKDRWNADQLLEH 971
             +CL  + +DR  + +LL+H
Sbjct: 1467 LRCLELQPQDRPPSRELLKH 1486

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 129,036,277
Number of Sequences: 369166
Number of extensions: 2735332
Number of successful extensions: 10174
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8285
length of database: 68,354,980
effective HSP length: 112
effective length of database: 47,664,660
effective search space used: 12011494320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

1. Dr_sW_025_P06
2. Dr_sW_009_I18
3. Dr_sW_002_B22
4. Dr_sW_027_K24