Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_P06
(751 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kina... 93 9e-19
sp|Q38997|KIN10_ARATH SNF1-related protein kinase catalytic... 91 3e-18
sp|P92958|KIN11_ARATH SNF1-related protein kinase catalytic... 90 6e-18
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 89 1e-17
sp|Q9Y7J6|KOIA_SCHPO Putative serine/threonine-protein kina... 89 1e-17
sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinas... 89 2e-17
sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinas... 88 3e-17
sp|P62343|CDPK1_PLAFK Calcium-dependent protein kinase 1 (P... 87 6e-17
sp|Q9Z2A0|PDPK1_MOUSE 3-phosphoinositide dependent protein ... 86 8e-17
sp|O15530|PDPK1_HUMAN 3-phosphoinositide dependent protein ... 86 8e-17
>sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kinase YOL100W
Length = 1081
Score = 92.8 bits (229), Expect = 9e-19
Identities = 49/155 (31%), Positives = 82/155 (52%), Gaps = 5/155 (3%)
Frame = +3
Query: 282 ILMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLGFFKDELYFYIAMEHMERGSLHFY 461
+L K Y + ++ K V E + KL++ P ++ L F+DE Y +E+ G
Sbjct: 209 VLNKEYLIRQKKVKYVSIEKTALQKLNNSPSVVRLFSTFQDESSLYFLLEYAPNGDFLSL 268
Query: 462 IKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYL 641
+K S++E R ++ QI+ + YLH N +IH D+K NILLD + IK++ F +K L
Sbjct: 269 MKKYGSLDETCARYYAAQIIDAIDYLHSNGIIHRDIKPENILLDGEMKIKLTDFGTAKLL 328
Query: 642 AGANSTVMSEGF-----MQSKPGTCNFMAPEVLID 731
N++V + +S GT +++PE+L D
Sbjct: 329 NPTNNSVSKPEYDLSTRSKSFVGTAEYVSPELLND 363
>sp|Q38997|KIN10_ARATH SNF1-related protein kinase catalytic alpha subunit KIN10 (AKIN10)
(AKIN alpha2)
Length = 535
Score = 90.9 bits (224), Expect = 3e-18
Identities = 53/172 (30%), Positives = 89/172 (51%), Gaps = 4/172 (2%)
Frame = +3
Query: 222 VAVGQFSQVYTAR--IVGNETVI-LMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLG 392
+ +G F +V A + G++ I ++ R + + +R + +L HP II L
Sbjct: 48 LGIGSFGRVKIAEHALTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRLYE 107
Query: 393 FFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLK 572
+ Y+ ME++ G L YI + ED R + QI+ G+ Y H+N V+H DLK
Sbjct: 108 VIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLK 167
Query: 573 SSNILLDNNLDIKISGFSLSKYLAGANSTVMSEG-FMQSKPGTCNFMAPEVL 725
N+LLD+ ++KI+ F L S +M +G F+++ G+ N+ APEV+
Sbjct: 168 PENLLLDSKCNVKIADFGL--------SNIMRDGHFLKTSCGSPNYAAPEVI 211
>sp|P92958|KIN11_ARATH SNF1-related protein kinase catalytic alpha subunit KIN11 (AKIN11)
(AKIN alpha1)
Length = 512
Score = 90.1 bits (222), Expect = 6e-18
Identities = 54/172 (31%), Positives = 89/172 (51%), Gaps = 4/172 (2%)
Frame = +3
Query: 222 VAVGQFSQVYTAR--IVGNETVI-LMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLG 392
+ +G F +V A + G++ I ++ R + + +R + +L HP II
Sbjct: 26 LGIGSFGKVKIAEHVVTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRQYE 85
Query: 393 FFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLK 572
+ Y+ ME+++ G L YI + ED R + QI+ G+ Y H+N V+H DLK
Sbjct: 86 VIETTSDIYVVMEYVKSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLK 145
Query: 573 SSNILLDNNLDIKISGFSLSKYLAGANSTVMSEG-FMQSKPGTCNFMAPEVL 725
N+LLD+ +IKI+ F L S VM +G F+++ G+ N+ APEV+
Sbjct: 146 PENLLLDSRCNIKIADFGL--------SNVMRDGHFLKTSCGSPNYAAPEVI 189
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 89.0 bits (219), Expect = 1e-17
Identities = 58/182 (31%), Positives = 91/182 (50%), Gaps = 6/182 (3%)
Frame = +3
Query: 198 ELIEGNNHVAVGQ--FSQVYTARIVGNETVILMKRYTVAEQSYKPVLRELVSFAKLDDHP 371
E E + V +G+ + VY R + N+ I +K + Y L E ++ K H
Sbjct: 676 EYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHK 735
Query: 372 RIINLLGFFKDELYFYIAMEHMERGSLHFYIKNN---SSINEDLIRKWSLQILQGLSYLH 542
I+ LG F + + I ME + GSL +++ NE I ++ QIL+GL YLH
Sbjct: 736 NIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLH 795
Query: 543 KNRVIHCDLKSSNILLDNNLDI-KISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 719
N+++H D+K N+L++ + KIS F SK LAG N ++ GT +MAPE
Sbjct: 796 DNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCT------ETFTGTLQYMAPE 849
Query: 720 VL 725
++
Sbjct: 850 II 851
>sp|Q9Y7J6|KOIA_SCHPO Putative serine/threonine-protein kinase C1778.10c
Length = 550
Score = 89.0 bits (219), Expect = 1e-17
Identities = 53/160 (33%), Positives = 83/160 (51%), Gaps = 5/160 (3%)
Frame = +3
Query: 282 ILMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLGFFKDELYFYIAMEHMERGSLHFY 461
+L K+Y V E K V E S +L+ P I L F+D+L Y +E G L Y
Sbjct: 84 VLDKKYIVKENKVKYVNIERDSMMRLNGFPGISRLFHTFQDDLKLYYVLELAPNGELLQY 143
Query: 462 IKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLKSSNILLDNNLDIKISGFSLS--- 632
IK ++E+ +R ++ +IL + Y+H +IH DLK NIL D N+ +KI+ F +
Sbjct: 144 IKKYRFLDENCVRFYAAEILSSIEYMHSCGIIHRDLKPENILFDGNMHVKITDFGTAKIL 203
Query: 633 --KYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITR 746
KY+ + T F+ GT ++APE+L Q +++
Sbjct: 204 PPKYVNSPDYTTFPSSFV----GTAEYVAPELLSRQVVSK 239
>sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1495
Score = 88.6 bits (218), Expect = 2e-17
Identities = 58/182 (31%), Positives = 98/182 (53%), Gaps = 7/182 (3%)
Frame = +3
Query: 222 VAVGQFSQVYTARIVGNETVILMKRYTVA------EQSYKPVLRELVSFAKLDDHPRIIN 383
+ +G FS Y A+ VG T++ +K+ T ++ LRE + +HP II
Sbjct: 1232 IGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIR 1291
Query: 384 LLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHC 563
+LG ++ + + +E M GS+ + + E ++ ++ Q+L+GLSYLH+N++IH
Sbjct: 1292 MLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHR 1351
Query: 564 DLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKI 740
D+K +N+L+D+ ++I+ F + LA + T E F GT FMAPEVL Q+
Sbjct: 1352 DVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEVLRGQQY 1409
Query: 741 TR 746
R
Sbjct: 1410 GR 1411
>sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1493
Score = 87.8 bits (216), Expect = 3e-17
Identities = 58/182 (31%), Positives = 98/182 (53%), Gaps = 7/182 (3%)
Frame = +3
Query: 222 VAVGQFSQVYTARIVGNETVILMKRYTVA------EQSYKPVLRELVSFAKLDDHPRIIN 383
+ +G FS Y A+ VG T++ +K+ T ++ LRE + +HP II
Sbjct: 1230 IGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIR 1289
Query: 384 LLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHC 563
+LG ++ + + +E M GS+ + + E ++ ++ Q+L+GLSYLH+N++IH
Sbjct: 1290 MLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHR 1349
Query: 564 DLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKI 740
D+K +N+L+D+ ++I+ F + LA + T E F GT FMAPEVL Q+
Sbjct: 1350 DVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEVLRGQQY 1407
Query: 741 TR 746
R
Sbjct: 1408 GR 1409
>sp|P62343|CDPK1_PLAFK Calcium-dependent protein kinase 1 (PfCPK) (PfCDPK1)
sp|P62344|CDPK1_PLAF7 Calcium-dependent protein kinase 1
Length = 524
Score = 86.7 bits (213), Expect = 6e-17
Identities = 51/131 (38%), Positives = 70/131 (53%), Gaps = 3/131 (2%)
Frame = +3
Query: 342 VSFAKLDDHPRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQIL 521
+S K DHP II L F+D+ YFY+ E E G L I N +E QIL
Sbjct: 117 ISLLKSLDHPNIIKLFDVFEDKKYFYLVTEFYEGGELFEQIINRHKFDECDAANIMKQIL 176
Query: 522 QGLSYLHKNRVIHCDLKSSNILLDNN---LDIKISGFSLSKYLAGANSTVMSEGFMQSKP 692
G+ YLHK+ ++H D+K NILL+N L+IKI F LS + + N ++ +
Sbjct: 177 SGICYLHKHNIVHRDIKPENILLENKHSLLNIKIVDFGLSSFFSKDNK-------LRDRL 229
Query: 693 GTCNFMAPEVL 725
GT ++APEVL
Sbjct: 230 GTAYYIAPEVL 240
>sp|Q9Z2A0|PDPK1_MOUSE 3-phosphoinositide dependent protein kinase 1 (mPDK1)
Length = 559
Score = 86.3 bits (212), Expect = 8e-17
Identities = 54/172 (31%), Positives = 88/172 (51%), Gaps = 4/172 (2%)
Frame = +3
Query: 231 GQFSQVYTARIVGNETV----ILMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLGFF 398
G FS V AR + IL KR+ + E V RE ++LD HP + L F
Sbjct: 94 GSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLD-HPFFVKLYFTF 152
Query: 399 KDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLKSS 578
+D+ Y + + + G L YI+ S +E R ++ +I+ L YLH +IH DLK
Sbjct: 153 QDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPE 212
Query: 579 NILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQ 734
NILL+ ++ I+I+ F +K L+ + + F+ GT +++PE+L ++
Sbjct: 213 NILLNEDMHIQITDFGTAKVLSPESKQARANSFV----GTAQYVSPELLTEK 260
>sp|O15530|PDPK1_HUMAN 3-phosphoinositide dependent protein kinase 1 (hPDK1)
Length = 556
Score = 86.3 bits (212), Expect = 8e-17
Identities = 54/172 (31%), Positives = 88/172 (51%), Gaps = 4/172 (2%)
Frame = +3
Query: 231 GQFSQVYTARIVGNETV----ILMKRYTVAEQSYKPVLRELVSFAKLDDHPRIINLLGFF 398
G FS V AR + IL KR+ + E V RE ++LD HP + L F
Sbjct: 91 GSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLD-HPFFVKLYFTF 149
Query: 399 KDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKNRVIHCDLKSS 578
+D+ Y + + + G L YI+ S +E R ++ +I+ L YLH +IH DLK
Sbjct: 150 QDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPE 209
Query: 579 NILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQ 734
NILL+ ++ I+I+ F +K L+ + + F+ GT +++PE+L ++
Sbjct: 210 NILLNEDMHIQITDFGTAKVLSPESKQARANSFV----GTAQYVSPELLTEK 257
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,067,164
Number of Sequences: 369166
Number of extensions: 1844358
Number of successful extensions: 7133
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6596
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6824907600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)