Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_K24
(732 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 129 6e-30
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 128 1e-29
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 125 2e-28
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 120 3e-27
sp|P27636|CDC15_YEAST Cell division control protein 15 119 6e-27
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 117 2e-26
sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kina... 117 3e-26
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 113 5e-25
sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinas... 112 1e-24
sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinas... 112 1e-24
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 129 bits (325), Expect = 6e-30
Identities = 78/206 (37%), Positives = 121/206 (58%), Gaps = 2/206 (0%)
Frame = +2
Query: 2 HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 175
H RI+ G +D E I ME+M GS+ +K ++ E + RK++ QIL+G+SYL
Sbjct: 421 HERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 176 HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 355
H N ++H D+K +NIL D+ ++K+ F SK L + MS M+S GT +M+PE
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGMRSVTGTPYWMSPE 537
Query: 356 VLIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpreGMSDHL 535
V+ + RK+DVWS G +VE++T K P+ E++ I KIA + T +P +S+H
Sbjct: 538 VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSH-ISEHG 596
Query: 536 RDFIAKCLTGKRKDRWNADQLLEHDF 613
RDF+ + R+ R +A++LL H F
Sbjct: 597 RDFLRRIFVEARQ-RPSAEELLTHHF 621
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 128 bits (322), Expect = 1e-29
Identities = 77/206 (37%), Positives = 121/206 (58%), Gaps = 2/206 (0%)
Frame = +2
Query: 2 HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 175
H RI+ G +D E I ME+M GS+ +K ++ E + RK++ QIL+G+SYL
Sbjct: 421 HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 176 HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 355
H N ++H D+K +NIL D+ ++K+ F SK L + MS ++S GT +M+PE
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPE 537
Query: 356 VLIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpreGMSDHL 535
V+ + RK+DVWS G +VE++T K P+ E++ I KIA + T +P +S+H
Sbjct: 538 VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSH-ISEHG 596
Query: 536 RDFIAKCLTGKRKDRWNADQLLEHDF 613
RDF+ + R+ R +A++LL H F
Sbjct: 597 RDFLRRIFVEARQ-RPSAEELLTHHF 621
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 125 bits (313), Expect = 2e-28
Identities = 76/209 (36%), Positives = 121/209 (57%), Gaps = 2/209 (0%)
Frame = +2
Query: 2 HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 175
H RI+ G +D E I ME+M GS+ +K ++ E+ RK++ QIL+G+ YL
Sbjct: 414 HERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYL 473
Query: 176 HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 355
H N ++H D+K +NIL D+ ++K+ F SK L + +S M+S GT +M+PE
Sbjct: 474 HSNMILHRDIKGANILRDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPE 530
Query: 356 VLIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpreGMSDHL 535
V+ Q RK+D+WS +VE++T K P+ E++ I KIA + T +P +SD+
Sbjct: 531 VISGQGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPH-VSDYT 589
Query: 536 RDFIAKCLTGKRKDRWNADQLLEHDFLKF 622
RDF+ + + K R +AD+LL H F+ +
Sbjct: 590 RDFLKRIFV-EAKLRPSADELLRHMFVHY 617
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 120 bits (302), Expect = 3e-27
Identities = 74/209 (35%), Positives = 120/209 (57%), Gaps = 2/209 (0%)
Frame = +2
Query: 2 HPRIINLLGFFKD--ELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYL 175
H RI+ G +D E I ME GS+ +K ++ E++ RK++ QIL+G+ YL
Sbjct: 415 HERIVQYYGCLRDPQEKTLSIFMELSPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYL 474
Query: 176 HKNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPE 355
H N ++H D+K +NIL D+ +IK+ F SK L + +S M+S GT +M+PE
Sbjct: 475 HSNMIVHRDIKGANILRDSTGNIKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPE 531
Query: 356 VLIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpreGMSDHL 535
V+ + RK+D+WS +VE++T K P+ E++ I KIA + T +P +SD+
Sbjct: 532 VISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPH-VSDYT 590
Query: 536 RDFIAKCLTGKRKDRWNADQLLEHDFLKF 622
RDF+ + + K R +A++LL H F+ +
Sbjct: 591 RDFLKRIFV-EAKLRPSAEELLRHMFVHY 618
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 119 bits (299), Expect = 6e-27
Identities = 69/203 (33%), Positives = 110/203 (54%), Gaps = 1/203 (0%)
Frame = +2
Query: 2 HPRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSS-INEDLIRKWSLQILQGLSYLH 178
H I+ GF + YI +E+ GSL I +S+ ++E+ + + Q L GL YLH
Sbjct: 79 HNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLH 138
Query: 179 KNRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEV 358
VIH D+K++NILL + +K++ F +S + + T+ GT N+MAPE+
Sbjct: 139 GEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSSALTLA---------GTLNWMAPEI 189
Query: 359 LIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpreGMSDHLR 538
L ++ + SD+WS GA +VE++T P++ L NI + ++ Y P S+ L+
Sbjct: 190 LGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAVEND---TYYPPSSFSEPLK 246
Query: 539 DFIAKCLTGKRKDRWNADQLLEH 607
DF++KC R ADQLL+H
Sbjct: 247 DFLSKCFVKNMYKRPTADQLLKH 269
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 117 bits (294), Expect = 2e-26
Identities = 74/211 (35%), Positives = 113/211 (53%), Gaps = 5/211 (2%)
Frame = +2
Query: 2 HPRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHK 181
H I+ LGF K E I +E++ GS+ ++N E L+R S Q+L GLSYLH
Sbjct: 889 HLNIVQYLGFEKTETDISIFLEYVSGGSIGRCLRNYGPFEEQLVRFVSRQVLYGLSYLHS 948
Query: 182 NRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVL 361
+IH DLK+ N+L+D + KIS F +SK+ + V S G+ +MAPEV+
Sbjct: 949 KGIIHRDLKADNLLIDFDGVCKISDFGISKH----SDNVYDNDANLSMQGSIFWMAPEVI 1004
Query: 362 ID--QKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpre---GMS 526
+ Q + K DVWS G V++E++ G++P+ E+I + K+ E+ +P E +S
Sbjct: 1005 HNDHQGYSAKVDVWSLGCVVLEMLAGRRPWSTDEAIQAMFKLGTEKKAPPIPSELVSQVS 1064
Query: 527 DHLRDFIAKCLTGKRKDRWNADQLLEHDFLK 619
F+ C T R A++LL H F+K
Sbjct: 1065 PEAIQFLNACFTVNADVRPTAEELLNHPFMK 1095
>sp|Q12236|KOK0_YEAST Probable serine/threonine-protein kinase YOL100W
Length = 1081
Score = 117 bits (293), Expect = 3e-26
Identities = 64/210 (30%), Positives = 103/210 (49%), Gaps = 5/210 (2%)
Frame = +2
Query: 5 PRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHKN 184
P ++ L F+DE Y +E+ G +K S++E R ++ QI+ + YLH N
Sbjct: 238 PSVVRLFSTFQDESSLYFLLEYAPNGDFLSLMKKYGSLDETCARYYAAQIIDAIDYLHSN 297
Query: 185 RVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGF-----MQSKPGTCNFMA 349
+IH D+K NILLD + IK++ F +K L N++V + +S GT +++
Sbjct: 298 GIIHRDIKPENILLDGEMKIKLTDFGTAKLLNPTNNSVSKPEYDLSTRSKSFVGTAEYVS 357
Query: 350 PEVLIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESINIILKIADEQTIDYVpreGMSD 529
PE+L D + D+W+FG +L +++ GK PF ++ N L + Y G
Sbjct: 358 PELLNDSFTDYRCDIWAFGCILFQMIAGKPPF---KATNEYLTFQKVMKVQYAFTPGFPL 414
Query: 530 HLRDFIAKCLTGKRKDRWNADQLLEHDFLK 619
+RD + K L R Q+ EH F K
Sbjct: 415 IIRDLVKKILVKNLDRRLTISQIKEHHFFK 444
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 113 bits (283), Expect = 5e-25
Identities = 66/206 (32%), Positives = 114/206 (55%), Gaps = 1/206 (0%)
Frame = +2
Query: 2 HPRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHK 181
HP I+ LG +++ I +E + GS+ ++ + E ++R ++ Q+L GL YLH
Sbjct: 130 HPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHN 189
Query: 182 NRVIHCDLKSSNILLDNNLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVL 361
+ ++H D+K +NIL+DN IK++ F SK +A + + +S GT +MAPEV+
Sbjct: 190 HAIMHRDIKGANILVDNQGCIKLADFGASKQVA----ELATISGAKSMKGTPYWMAPEVI 245
Query: 362 IDQKITRKSDVWSFGAVLVELVTGKQPF-YGLESINIILKIADEQTIDYVpreGMSDHLR 538
+ + +D+WS G ++E+VTGK P+ + I I I ++ +P + +S
Sbjct: 246 LQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIP-DNISSDAN 304
Query: 539 DFIAKCLTGKRKDRWNADQLLEHDFL 616
DF+ KCL + R A +LL+H F+
Sbjct: 305 DFLLKCLQQEPNLRPTASELLKHPFV 330
>sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1493
Score = 112 bits (280), Expect = 1e-24
Identities = 71/206 (34%), Positives = 114/206 (55%), Gaps = 4/206 (1%)
Frame = +2
Query: 2 HPRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHK 181
HP II +LG ++ + + +E M GS+ + + E ++ ++ Q+L+GLSYLH+
Sbjct: 1284 HPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHE 1343
Query: 182 NRVIHCDLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEV 358
N++IH D+K +N+L+D+ ++I+ F + LA + T E F GT FMAPEV
Sbjct: 1344 NQIIHRDVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEV 1401
Query: 359 LIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESIN---IILKIADEQTIDYVpreGMSD 529
L Q+ R DVWS G ++E+ K P+ + N +I KIA T +P +S
Sbjct: 1402 LRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSH-LSP 1460
Query: 530 HLRDFIAKCLTGKRKDRWNADQLLEH 607
LRD +CL + +DR + +LL+H
Sbjct: 1461 GLRDVAVRCLELQPQDRPPSRELLKH 1486
>sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1495
Score = 112 bits (279), Expect = 1e-24
Identities = 71/206 (34%), Positives = 114/206 (55%), Gaps = 4/206 (1%)
Frame = +2
Query: 2 HPRIINLLGFFKDELYFYIAMEHMERGSLHFYIKNNSSINEDLIRKWSLQILQGLSYLHK 181
HP II +LG ++ + + +E M GS+ + + E ++ ++ Q+L+GLSYLH+
Sbjct: 1286 HPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHE 1345
Query: 182 NRVIHCDLKSSNILLDN-NLDIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEV 358
N++IH D+K +N+L+D+ ++I+ F + LA + T E F GT FMAPEV
Sbjct: 1346 NQIIHRDVKGANLLIDSTGQRLRIADFGAAARLA-SKGTGAGE-FQGQLLGTIAFMAPEV 1403
Query: 359 LIDQKITRKSDVWSFGAVLVELVTGKQPFYGLESIN---IILKIADEQTIDYVpreGMSD 529
L Q+ R DVWS G ++E+ K P+ + N +I KIA T +P +S
Sbjct: 1404 LRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSH-LSP 1462
Query: 530 HLRDFIAKCLTGKRKDRWNADQLLEH 607
LRD +CL + +DR + +LL+H
Sbjct: 1463 GLRDVALRCLELQPQDRPPSRELLKH 1488
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,819,609
Number of Sequences: 369166
Number of extensions: 1768584
Number of successful extensions: 8095
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5988
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6281
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)