Planaria EST Database

DrC_01008

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01008
         (707 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q75C43|SNX4_ASHGO  Sorting nexin-4 (Autophagy-related pro...    35   0.15 
sp|P07271|PIF1_YEAST  DNA repair and recombination protein P...    33   0.57 
sp|Q4R5U9|SXN7_MACFA  Sorting nexin-7                              33   0.74 
sp|Q9UNH6|SNX7_HUMAN  Sorting nexin-7                              33   0.97 
sp|Q7N365|YS56_PHOLL  Hypothetical UPF0324 membrane protein ...    32   1.3  
sp|P47057|SNX4_YEAST  Sorting nexin-4 (Autophagy-related pro...    32   1.7  
sp|Q6CTQ0|SNX4_KLULA  Sorting nexin-4 (Autophagy-related pro...    32   1.7  
sp|Q9ZYM7|NU5M_RHISA  NADH-ubiquinone oxidoreductase chain 5...    32   2.2  
sp|Q9CY18|SNX7_MOUSE  Sorting nexin-7                              31   2.8  
sp|P40007|YEJ2_YEAST  Hypothetical 26.9 kDa protein in MNN1-...    31   2.8  

>sp|Q75C43|SNX4_ASHGO Sorting nexin-4 (Autophagy-related protein 24)
          Length = 410

 Score = 35.4 bits (80), Expect = 0.15
 Identities = 20/75 (26%), Positives = 37/75 (49%)
 Frame = +1

Query: 247 QSKIQDQNEDISNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFADL 426
           +SK+Q    ++ N       A K+ D   K   + + E++ +++ +  E K  L   AD 
Sbjct: 342 ESKVQALTTEVEN-------AKKVADAFEK---EALKEVEIFEQIKTRELKRSLTTLADH 391

Query: 427 HIDHYRQMSTIWRKV 471
           HI+ Y++M   W K+
Sbjct: 392 HIEFYQKMVNTWSKI 406

>sp|P07271|PIF1_YEAST DNA repair and recombination protein PIF1, mitochondrial precursor
           (ATP-dependent helicase PIF1)
          Length = 857

 Score = 33.5 bits (75), Expect = 0.57
 Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
 Frame = +1

Query: 280 SNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRC---EETKEWLKVFADLH 429
           SN   Q Q+  K   I+SK + DL+S+ D W+E  C   E  K+  K+  D+H
Sbjct: 47  SNNFIQAQL--KHPSILSKEDLDLLSDSDDWEEPDCIQLETEKQEKKIITDIH 97

>sp|Q4R5U9|SXN7_MACFA Sorting nexin-7
          Length = 387

 Score = 33.1 bits (74), Expect = 0.74
 Identities = 26/111 (23%), Positives = 46/111 (41%)
 Frame = +1

Query: 148 PWIVESSLYWTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAQQGQVANKLHDI 327
           P + E  LY   +  V +RR+  +A+        SK++      ++     +   KL D 
Sbjct: 269 PVVREYVLYSEMLMGVMKRRDQIQAEL------DSKVEALTYKKTDTDLLPEEIGKLEDK 322

Query: 328 VSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 480
           V   N  L ++ +RWK+    + K      A+ +I +Y Q    W   + S
Sbjct: 323 VECANNALKADWERWKQNMQNDIKLAFTDMAEENIHYYEQCLATWESFLTS 373

>sp|Q9UNH6|SNX7_HUMAN Sorting nexin-7
          Length = 387

 Score = 32.7 bits (73), Expect = 0.97
 Identities = 26/111 (23%), Positives = 46/111 (41%)
 Frame = +1

Query: 148 PWIVESSLYWTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAQQGQVANKLHDI 327
           P + E  LY   +  V +RR+  +A+        SK++      ++     +   KL D 
Sbjct: 269 PVVHEYVLYSEMLMGVMKRRDQIQAEL------DSKVEVLTYKKADTDLLPEEIGKLEDK 322

Query: 328 VSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 480
           V   N  L ++ +RWK+    + K      A+ +I +Y Q    W   + S
Sbjct: 323 VECANNALKADWERWKQNMQNDIKLAFTDMAEENIHYYEQCLATWESFLTS 373

>sp|Q7N365|YS56_PHOLL Hypothetical UPF0324 membrane protein plu2856
          Length = 360

 Score = 32.3 bits (72), Expect = 1.3
 Identities = 13/52 (25%), Positives = 28/52 (53%)
 Frame = +2

Query: 89  HHHWEINTNNQLKIFNGKSNHGLLRVVSIGHKFSRLLDDATMLRQNLSLVLL 244
           H+ W   T     IF G + H + +VV++GH  S   ++  ++ + + +++L
Sbjct: 194 HYQWLPLTQETFGIFTGSTLHEVAQVVAVGHAISDQTENVAVISKMIRVMML 245

>sp|P47057|SNX4_YEAST Sorting nexin-4 (Autophagy-related protein 24) (Cytoplasm to
           vacuole targeting protein 13)
          Length = 423

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 20/79 (25%), Positives = 35/79 (44%), Gaps = 14/79 (17%)
 Frame = +1

Query: 277 ISNQAQQGQVANKLHDIVSKNNADL--------------ISEIDRWKEQRCEETKEWLKV 414
           I+ +A + +  NKL   ++    +L              + EID ++  +  E K+ L  
Sbjct: 341 INQEASRREKINKLEGKITSLTGELENAKKVADGFEQECLKEIDHFESVKTAEIKKSLGS 400

Query: 415 FADLHIDHYRQMSTIWRKV 471
            AD HI+ Y ++   W KV
Sbjct: 401 LADHHIEFYERILEAWEKV 419

>sp|Q6CTQ0|SNX4_KLULA Sorting nexin-4 (Autophagy-related protein 24)
          Length = 400

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 14/76 (18%), Positives = 40/76 (52%)
 Frame = +1

Query: 244 KQSKIQDQNEDISNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFAD 423
           K +K++ + + ++++ ++ +       +  +   +++ E++++++ +  E K+ L   A 
Sbjct: 324 KIAKLETRVQSLTDEVEKSK------QVADEFEKEVLKEVEQFEQIKTLELKDSLAALAQ 377

Query: 424 LHIDHYRQMSTIWRKV 471
            HID Y  M   W K+
Sbjct: 378 KHIDFYDDMVEKWSKI 393

>sp|Q9ZYM7|NU5M_RHISA NADH-ubiquinone oxidoreductase chain 5 (NADH dehydrogenase subunit
           5)
          Length = 552

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 21/47 (44%), Positives = 27/47 (57%)
 Frame = -3

Query: 324 VMKFICHLSLLCLI*NVFILILNFALFNNTSDKFCLSIVASSSNLLN 184
           V+ FI  + LL L+ NVF+LIL +     TS  +CL I   SSN  N
Sbjct: 89  VLMFILSMLLLVLMPNVFMLILGWDGLGLTS--YCLVIFYQSSNSYN 133

>sp|Q9CY18|SNX7_MOUSE Sorting nexin-7
          Length = 387

 Score = 31.2 bits (69), Expect = 2.8
 Identities = 28/115 (24%), Positives = 48/115 (41%), Gaps = 4/115 (3%)
 Frame = +1

Query: 148 PWIVESSLYWTQVQQVARRRN----DAEAKFIASIVKQSKIQDQNEDISNQAQQGQVANK 315
           P + E  LY   +  V +RR+    + ++K  A   K++ I    E+I           K
Sbjct: 269 PVVHEYVLYSEMLVGVMKRRDQIQTELDSKVEALTYKKADIDLLTEEIG----------K 318

Query: 316 LHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 480
           L D V   N  L ++ +RWK+    + +      A+ +I +Y Q    W   + S
Sbjct: 319 LEDKVECANNALKADWERWKQNMKNDLRSAFTDTAEQNIRYYEQCLATWESFLTS 373

>sp|P40007|YEJ2_YEAST Hypothetical 26.9 kDa protein in MNN1-PMI40 intergenic region
          Length = 231

 Score = 31.2 bits (69), Expect = 2.8
 Identities = 20/90 (22%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
 Frame = +1

Query: 196 ARRRNDAEAKFIASIVKQSKIQDQNEDIS-----NQAQQGQVANKLHDIVSKNNADLISE 360
           AR + D     +  +  + K  D +ED++     +  ++ +V  KL ++ S+        
Sbjct: 117 ARIQRDKNGDVVRVVYGKKKNFDADEDVNEIKARDTTEETEVVKKLEELASR-------P 169

Query: 361 IDRWKEQRCEETKEWLKVFADLHIDHYRQM 450
           + R +  + E  +EWL+     H D Y++M
Sbjct: 170 VIRKERSQSEREEEWLEKLYKKHGDDYKKM 199

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,442,439
Number of Sequences: 369166
Number of extensions: 1349837
Number of successful extensions: 3889
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3888
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6219306880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01008

  1. Dr_sW_025_N05
  2. Dr_sW_025_H12
  3. Dr_sW_006_H08
  4. Dr_sW_023_F11
  5. Dr_sW_007_H11