Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_F11
(628 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q75C43|SNX4_ASHGO Sorting nexin-4 (Autophagy-related pro... 37 0.054
sp|P07271|PIF1_YEAST DNA repair and recombination protein P... 33 0.46
sp|Q6CTQ0|SNX4_KLULA Sorting nexin-4 (Autophagy-related pro... 33 0.60
sp|Q4R5U9|SXN7_MACFA Sorting nexin-7 33 0.78
sp|Q9UNH6|SNX7_HUMAN Sorting nexin-7 32 1.0
sp|P12844|MYO3_CAEEL Myosin-3 (Myosin heavy chain A) (MHC A) 32 1.0
sp|Q7N365|YS56_PHOLL Hypothetical UPF0324 membrane protein ... 32 1.0
sp|P40007|YEJ2_YEAST Hypothetical 26.9 kDa protein in MNN1-... 32 1.0
sp|P47057|SNX4_YEAST Sorting nexin-4 (Autophagy-related pro... 32 1.3
sp|Q9CY18|SNX7_MOUSE Sorting nexin-7 31 2.3
>sp|Q75C43|SNX4_ASHGO Sorting nexin-4 (Autophagy-related protein 24)
Length = 410
Score = 36.6 bits (83), Expect = 0.054
Identities = 25/99 (25%), Positives = 47/99 (47%)
Frame = +3
Query: 96 WTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAEQGQVANKLHDIVSKNNADLI 275
+T + Q A RR K SK++ + + ++ + E A K+ D K + +
Sbjct: 325 FTGINQEAARRE-----------KISKLESKVQALTTEVEN---AKKVADAFEK---EAL 367
Query: 276 SEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKV 392
E++ +++ + E K L AD HI+ Y++M W K+
Sbjct: 368 KEVEIFEQIKTRELKRSLTTLADHHIEFYQKMVNTWSKI 406
>sp|P07271|PIF1_YEAST DNA repair and recombination protein PIF1, mitochondrial precursor
(ATP-dependent helicase PIF1)
Length = 857
Score = 33.5 bits (75), Expect = 0.46
Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
Frame = +3
Query: 201 SNQAEQGQVANKLHDIVSKNNADLISEIDRWKEQRC---EETKEWLKVFADLH 350
SN Q Q+ K I+SK + DL+S+ D W+E C E K+ K+ D+H
Sbjct: 47 SNNFIQAQL--KHPSILSKEDLDLLSDSDDWEEPDCIQLETEKQEKKIITDIH 97
>sp|Q6CTQ0|SNX4_KLULA Sorting nexin-4 (Autophagy-related protein 24)
Length = 400
Score = 33.1 bits (74), Expect = 0.60
Identities = 15/76 (19%), Positives = 40/76 (52%)
Frame = +3
Query: 165 KQSKIQDQNEDISNQAEQGQVANKLHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFAD 344
K +K++ + + ++++ E+ + + + +++ E++++++ + E K+ L A
Sbjct: 324 KIAKLETRVQSLTDEVEKSK------QVADEFEKEVLKEVEQFEQIKTLELKDSLAALAQ 377
Query: 345 LHIDHYRQMSTIWRKV 392
HID Y M W K+
Sbjct: 378 KHIDFYDDMVEKWSKI 393
>sp|Q4R5U9|SXN7_MACFA Sorting nexin-7
Length = 387
Score = 32.7 bits (73), Expect = 0.78
Identities = 26/111 (23%), Positives = 46/111 (41%)
Frame = +3
Query: 69 PWIVESSLYWTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAEQGQVANKLHDI 248
P + E LY + V +RR+ +A+ SK++ ++ + KL D
Sbjct: 269 PVVREYVLYSEMLMGVMKRRDQIQAEL------DSKVEALTYKKTDTDLLPEEIGKLEDK 322
Query: 249 VSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 401
V N L ++ +RWK+ + K A+ +I +Y Q W + S
Sbjct: 323 VECANNALKADWERWKQNMQNDIKLAFTDMAEENIHYYEQCLATWESFLTS 373
>sp|Q9UNH6|SNX7_HUMAN Sorting nexin-7
Length = 387
Score = 32.3 bits (72), Expect = 1.0
Identities = 26/111 (23%), Positives = 46/111 (41%)
Frame = +3
Query: 69 PWIVESSLYWTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAEQGQVANKLHDI 248
P + E LY + V +RR+ +A+ SK++ ++ + KL D
Sbjct: 269 PVVHEYVLYSEMLMGVMKRRDQIQAEL------DSKVEVLTYKKADTDLLPEEIGKLEDK 322
Query: 249 VSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 401
V N L ++ +RWK+ + K A+ +I +Y Q W + S
Sbjct: 323 VECANNALKADWERWKQNMQNDIKLAFTDMAEENIHYYEQCLATWESFLTS 373
>sp|P12844|MYO3_CAEEL Myosin-3 (Myosin heavy chain A) (MHC A)
Length = 1969
Score = 32.3 bits (72), Expect = 1.0
Identities = 19/86 (22%), Positives = 45/86 (52%), Gaps = 4/86 (4%)
Frame = +3
Query: 102 QVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAEQGQVANKLHDI----VSKNNAD 269
Q+++ R++++ +F+AS + + +Q + ++++ QAE + A + + + + N D
Sbjct: 1669 QIEEEQRQKDEIREQFLASEKRNAILQSEKDELAQQAEAAERARRNAEAECIELREQNND 1728
Query: 270 LISEIDRWKEQRCEETKEWLKVFADL 347
L + + QR + E L A+L
Sbjct: 1729 LNAHVSALTGQRRKLEGELLAAHAEL 1754
Score = 29.6 bits (65), Expect = 6.6
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 5/73 (6%)
Frame = +3
Query: 150 IASIVKQSKIQDQ-----NEDISNQAEQGQVANKLHDIVSKNNADLISEIDRWKEQRCEE 314
+A + K+ K Q++ NED+ ++ ++ K+ + + + +L IDR K R +
Sbjct: 1002 VAKLNKEKKHQEESNRKLNEDLQSEEDKVNHLEKIRNKLEQQMDELEENIDREKRSRGDI 1061
Query: 315 TKEWLKVFADLHI 353
K KV DL +
Sbjct: 1062 EKAKRKVEGDLKV 1074
>sp|Q7N365|YS56_PHOLL Hypothetical UPF0324 membrane protein plu2856
Length = 360
Score = 32.3 bits (72), Expect = 1.0
Identities = 13/52 (25%), Positives = 28/52 (53%)
Frame = +1
Query: 10 HHHWEINTNNQLKIFNGKSNHGLLRVVSIGHKFSRLLDDATMLRQNLSLVLL 165
H+ W T IF G + H + +VV++GH S ++ ++ + + +++L
Sbjct: 194 HYQWLPLTQETFGIFTGSTLHEVAQVVAVGHAISDQTENVAVISKMIRVMML 245
>sp|P40007|YEJ2_YEAST Hypothetical 26.9 kDa protein in MNN1-PMI40 intergenic region
Length = 231
Score = 32.3 bits (72), Expect = 1.0
Identities = 21/90 (23%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
Frame = +3
Query: 117 ARRRNDAEAKFIASIVKQSKIQDQNEDIS-----NQAEQGQVANKLHDIVSKNNADLISE 281
AR + D + + + K D +ED++ + E+ +V KL ++ S+
Sbjct: 117 ARIQRDKNGDVVRVVYGKKKNFDADEDVNEIKARDTTEETEVVKKLEELASR-------P 169
Query: 282 IDRWKEQRCEETKEWLKVFADLHIDHYRQM 371
+ R + + E +EWL+ H D Y++M
Sbjct: 170 VIRKERSQSEREEEWLEKLYKKHGDDYKKM 199
>sp|P47057|SNX4_YEAST Sorting nexin-4 (Autophagy-related protein 24) (Cytoplasm to
vacuole targeting protein 13)
Length = 423
Score = 32.0 bits (71), Expect = 1.3
Identities = 20/79 (25%), Positives = 35/79 (44%), Gaps = 14/79 (17%)
Frame = +3
Query: 198 ISNQAEQGQVANKLHDIVSKNNADL--------------ISEIDRWKEQRCEETKEWLKV 335
I+ +A + + NKL ++ +L + EID ++ + E K+ L
Sbjct: 341 INQEASRREKINKLEGKITSLTGELENAKKVADGFEQECLKEIDHFESVKTAEIKKSLGS 400
Query: 336 FADLHIDHYRQMSTIWRKV 392
AD HI+ Y ++ W KV
Sbjct: 401 LADHHIEFYERILEAWEKV 419
>sp|Q9CY18|SNX7_MOUSE Sorting nexin-7
Length = 387
Score = 31.2 bits (69), Expect = 2.3
Identities = 28/115 (24%), Positives = 48/115 (41%), Gaps = 4/115 (3%)
Frame = +3
Query: 69 PWIVESSLYWTQVQQVARRRN----DAEAKFIASIVKQSKIQDQNEDISNQAEQGQVANK 236
P + E LY + V +RR+ + ++K A K++ I E+I K
Sbjct: 269 PVVHEYVLYSEMLVGVMKRRDQIQTELDSKVEALTYKKADIDLLTEEIG----------K 318
Query: 237 LHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 401
L D V N L ++ +RWK+ + + A+ +I +Y Q W + S
Sbjct: 319 LEDKVECANNALKADWERWKQNMKNDLRSAFTDTAEQNIRYYEQCLATWESFLTS 373
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,161,642
Number of Sequences: 369166
Number of extensions: 1135520
Number of successful extensions: 3376
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3375
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)