Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_N05
(486 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q75C43|SNX4_ASHGO Sorting nexin-4 (Autophagy-related pro... 35 0.070
sp|P07271|PIF1_YEAST DNA repair and recombination protein P... 33 0.27
sp|Q4R5U9|SXN7_MACFA Sorting nexin-7 33 0.35
sp|Q9UNH6|SNX7_HUMAN Sorting nexin-7 33 0.46
sp|P47057|SNX4_YEAST Sorting nexin-4 (Autophagy-related pro... 32 0.78
sp|Q6CTQ0|SNX4_KLULA Sorting nexin-4 (Autophagy-related pro... 32 0.78
sp|Q9CY18|SNX7_MOUSE Sorting nexin-7 31 1.3
sp|P40007|YEJ2_YEAST Hypothetical 26.9 kDa protein in MNN1-... 31 1.3
sp|Q9ZYM7|NU5M_RHISA NADH-ubiquinone oxidoreductase chain 5... 31 1.3
sp|P12222|YCF1_TOBAC Hypothetical 226 kDa protein ycf1 (ORF... 31 1.7
>sp|Q75C43|SNX4_ASHGO Sorting nexin-4 (Autophagy-related protein 24)
Length = 410
Score = 35.4 bits (80), Expect = 0.070
Identities = 20/75 (26%), Positives = 37/75 (49%)
Frame = +2
Query: 128 QSKIQDQNEDISNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFADL 307
+SK+Q ++ N A K+ D K + + E++ +++ + E K L AD
Sbjct: 342 ESKVQALTTEVEN-------AKKVADAFEK---EALKEVEIFEQIKTRELKRSLTTLADH 391
Query: 308 HIDHYRQMSTIWRKV 352
HI+ Y++M W K+
Sbjct: 392 HIEFYQKMVNTWSKI 406
>sp|P07271|PIF1_YEAST DNA repair and recombination protein PIF1, mitochondrial precursor
(ATP-dependent helicase PIF1)
Length = 857
Score = 33.5 bits (75), Expect = 0.27
Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
Frame = +2
Query: 161 SNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRC---EETKEWLKVFADLH 310
SN Q Q+ K I+SK + DL+S+ D W+E C E K+ K+ D+H
Sbjct: 47 SNNFIQAQL--KHPSILSKEDLDLLSDSDDWEEPDCIQLETEKQEKKIITDIH 97
>sp|Q4R5U9|SXN7_MACFA Sorting nexin-7
Length = 387
Score = 33.1 bits (74), Expect = 0.35
Identities = 26/111 (23%), Positives = 46/111 (41%)
Frame = +2
Query: 29 PWIVESSLYWTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAQQGQVANKLHDI 208
P + E LY + V +RR+ +A+ SK++ ++ + KL D
Sbjct: 269 PVVREYVLYSEMLMGVMKRRDQIQAEL------DSKVEALTYKKTDTDLLPEEIGKLEDK 322
Query: 209 VSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 361
V N L ++ +RWK+ + K A+ +I +Y Q W + S
Sbjct: 323 VECANNALKADWERWKQNMQNDIKLAFTDMAEENIHYYEQCLATWESFLTS 373
>sp|Q9UNH6|SNX7_HUMAN Sorting nexin-7
Length = 387
Score = 32.7 bits (73), Expect = 0.46
Identities = 26/111 (23%), Positives = 46/111 (41%)
Frame = +2
Query: 29 PWIVESSLYWTQVQQVARRRNDAEAKFIASIVKQSKIQDQNEDISNQAQQGQVANKLHDI 208
P + E LY + V +RR+ +A+ SK++ ++ + KL D
Sbjct: 269 PVVHEYVLYSEMLMGVMKRRDQIQAEL------DSKVEVLTYKKADTDLLPEEIGKLEDK 322
Query: 209 VSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 361
V N L ++ +RWK+ + K A+ +I +Y Q W + S
Sbjct: 323 VECANNALKADWERWKQNMQNDIKLAFTDMAEENIHYYEQCLATWESFLTS 373
>sp|P47057|SNX4_YEAST Sorting nexin-4 (Autophagy-related protein 24) (Cytoplasm to
vacuole targeting protein 13)
Length = 423
Score = 32.0 bits (71), Expect = 0.78
Identities = 20/79 (25%), Positives = 35/79 (44%), Gaps = 14/79 (17%)
Frame = +2
Query: 158 ISNQAQQGQVANKLHDIVSKNNADL--------------ISEIDRWKEQRCEETKEWLKV 295
I+ +A + + NKL ++ +L + EID ++ + E K+ L
Sbjct: 341 INQEASRREKINKLEGKITSLTGELENAKKVADGFEQECLKEIDHFESVKTAEIKKSLGS 400
Query: 296 FADLHIDHYRQMSTIWRKV 352
AD HI+ Y ++ W KV
Sbjct: 401 LADHHIEFYERILEAWEKV 419
>sp|Q6CTQ0|SNX4_KLULA Sorting nexin-4 (Autophagy-related protein 24)
Length = 400
Score = 32.0 bits (71), Expect = 0.78
Identities = 14/76 (18%), Positives = 40/76 (52%)
Frame = +2
Query: 125 KQSKIQDQNEDISNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFAD 304
K +K++ + + ++++ ++ + + + +++ E++++++ + E K+ L A
Sbjct: 324 KIAKLETRVQSLTDEVEKSK------QVADEFEKEVLKEVEQFEQIKTLELKDSLAALAQ 377
Query: 305 LHIDHYRQMSTIWRKV 352
HID Y M W K+
Sbjct: 378 KHIDFYDDMVEKWSKI 393
>sp|Q9CY18|SNX7_MOUSE Sorting nexin-7
Length = 387
Score = 31.2 bits (69), Expect = 1.3
Identities = 28/115 (24%), Positives = 48/115 (41%), Gaps = 4/115 (3%)
Frame = +2
Query: 29 PWIVESSLYWTQVQQVARRRN----DAEAKFIASIVKQSKIQDQNEDISNQAQQGQVANK 196
P + E LY + V +RR+ + ++K A K++ I E+I K
Sbjct: 269 PVVHEYVLYSEMLVGVMKRRDQIQTELDSKVEALTYKKADIDLLTEEIG----------K 318
Query: 197 LHDIVSKNNADLISEIDRWKEQRCEETKEWLKVFADLHIDHYRQMSTIWRKVVMS 361
L D V N L ++ +RWK+ + + A+ +I +Y Q W + S
Sbjct: 319 LEDKVECANNALKADWERWKQNMKNDLRSAFTDTAEQNIRYYEQCLATWESFLTS 373
>sp|P40007|YEJ2_YEAST Hypothetical 26.9 kDa protein in MNN1-PMI40 intergenic region
Length = 231
Score = 31.2 bits (69), Expect = 1.3
Identities = 20/90 (22%), Positives = 41/90 (45%), Gaps = 5/90 (5%)
Frame = +2
Query: 77 ARRRNDAEAKFIASIVKQSKIQDQNEDIS-----NQAQQGQVANKLHDIVSKNNADLISE 241
AR + D + + + K D +ED++ + ++ +V KL ++ S+
Sbjct: 117 ARIQRDKNGDVVRVVYGKKKNFDADEDVNEIKARDTTEETEVVKKLEELASR-------P 169
Query: 242 IDRWKEQRCEETKEWLKVFADLHIDHYRQM 331
+ R + + E +EWL+ H D Y++M
Sbjct: 170 VIRKERSQSEREEEWLEKLYKKHGDDYKKM 199
>sp|Q9ZYM7|NU5M_RHISA NADH-ubiquinone oxidoreductase chain 5 (NADH dehydrogenase subunit
5)
Length = 552
Score = 31.2 bits (69), Expect = 1.3
Identities = 21/47 (44%), Positives = 27/47 (57%)
Frame = -3
Query: 205 VMKFICHLPLLCLI*NVFILILNFALFNNTSDKFCLSIVASSSNLLN 65
V+ FI + LL L+ NVF+LIL + TS +CL I SSN N
Sbjct: 89 VLMFILSMLLLVLMPNVFMLILGWDGLGLTS--YCLVIFYQSSNSYN 133
>sp|P12222|YCF1_TOBAC Hypothetical 226 kDa protein ycf1 (ORF 1901)
Length = 1901
Score = 30.8 bits (68), Expect = 1.7
Identities = 28/113 (24%), Positives = 51/113 (45%), Gaps = 19/113 (16%)
Frame = +2
Query: 17 WKIEPWIVESSLYWTQV-------QQVARRRNDAEAKFIASIVK-QSKIQDQNE------ 154
+KI+PWI+ S L + Q +N + F+ S K +++IQ+ E
Sbjct: 1543 YKIKPWIIPSKLLLLNLNTNENVSQNKNINKNQKQNFFLRSNKKIKNRIQEAKEPASQGE 1602
Query: 155 -----DISNQAQQGQVANKLHDIVSKNNADLISEIDRWKEQRCEETKEWLKVF 298
DI N+ G V +K + + K+ A+ ++ + K+Q T+ L +F
Sbjct: 1603 KERGSDIENKGNLGPVLSKHQNALKKDYAESDTKKGKKKKQYKSNTEAELDLF 1655
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,084,165
Number of Sequences: 369166
Number of extensions: 916965
Number of successful extensions: 2748
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2747
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2921208590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)