Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_00933 (980 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q8UVX0|PIWI_BRARE Piwi protein 180 5e-45 sp|Q9VKM1|PIWI_DROME Piwi protein 168 2e-41 sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein 102 2e-21 sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromo... 102 2e-21 sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 96 2e-19 sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 96 2e-19 sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation fact... 95 3e-19 sp|O77503|I2C2_RABIT Eukaryotic translation initiation fact... 95 3e-19 sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation fact... 95 3e-19 sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation fact... 95 3e-19
>sp|Q8UVX0|PIWI_BRARE Piwi protein Length = 858 Score = 180 bits (457), Expect = 5e-45 Identities = 109/297 (36%), Positives = 168/297 (56%), Gaps = 6/297 (2%) Frame = +2 Query: 32 TQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLKMAPTMIVGIDTFH-SKTG 208 +QCV R S + TVA K +QM K+G + W + + + MIVGID +H + G Sbjct: 580 SQCVVSRTISK--PQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAG 637 Query: 209 KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIY 388 KRS+ A V S++ S++ S E + L + A+ A+ + ++P R I+Y Sbjct: 638 KRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVY 697 Query: 389 RDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFFK--DG--ANP 556 RDGVGD L +E +M+ ++ + ++ P++ VVVKKRIS +FF DG ANP Sbjct: 698 RDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANP 756 Query: 557 NPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQ 736 PGTV+D ++ +P +Y+F++VSQ G +PT+YNV+ D S + P +Q Sbjct: 757 PPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQ 806 Query: 737 QITYSLTHLYFNWMGTIRVPVPVHYADRLAELVG-IVHRGTAPPTINERIRERLFYL 904 ++TY L H+Y+NW G +RVP P YA +LA LVG +H+ P +N + + L+YL Sbjct: 807 RLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHK---EPNMN--LDDFLYYL 858
>sp|Q9VKM1|PIWI_DROME Piwi protein Length = 843 Score = 168 bits (425), Expect = 2e-41 Identities = 103/294 (35%), Positives = 153/294 (52%), Gaps = 4/294 (1%) Frame = +2 Query: 32 TQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLKMAPTMIVGIDTFHS-KTG 208 TQ VT + NR ++A K +Q+ KLGY PW I L ++ M +G D S + Sbjct: 566 TQVVTLKTTKNRS---LMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDR 622 Query: 209 KRSVQASVFSIS-SKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFII 385 KR+ A + S+ + S Y S V + L A+ +Q + +P R + Sbjct: 623 KRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVF 682 Query: 386 YRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNL--PQIIYVVVKKRISVKFFKDGANPN 559 YRDGV L +FE +++ ++ Y L PQ+ Y+VV + ++ +FF +G NP Sbjct: 683 YRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPP 742 Query: 560 PGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQ 739 PGT+VD+ I P Y+FYLVSQ+ +GT SPT+YNVL + ++P +Q+ Sbjct: 743 PGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSS----------MGLSPEKMQK 792 Query: 740 ITYSLTHLYFNWMGTIRVPVPVHYADRLAELVGIVHRGTAPPTINERIRERLFY 901 +TY + HLY+NW GT RVP YA +LA LV GT +I + E+ FY Sbjct: 793 LTYKMCHLYYNWSGTTRVPAVCQYAKKLATLV-----GTNLHSIPQNALEKKFY 841
>sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein Length = 1214 Score = 102 bits (254), Expect = 2e-21 Identities = 84/299 (28%), Positives = 134/299 (44%), Gaps = 21/299 (7%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLK---------MAPTMI 175 G+LTQC+ Q + + + ++++ SKL GI+ K M TM Sbjct: 909 GILTQCIKQFTVERKCNNQ---TIGNILLKINSKLN----GINHKIKDDPRLPMMKNTMY 961 Query: 176 VGIDTFHSKTGKRSVQASVFSISSKFSQYMSFVNSP----KGKNEFHENLGKNFLTAITA 343 +G D H +R + SV +++ Y + N +G E E++ L + Sbjct: 962 IGADVTHPSPDQREIP-SVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRV 1020 Query: 344 FQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRI 523 +++ P I YRDGV D Q K E + + +K+ P+I V+V KR Sbjct: 1021 YKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCK---PKICCVIVVKRH 1077 Query: 524 SVKFFKDGA--------NPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDT 679 +FF G N +PGTVVD IV PN +F++VS + +GTA PT YNV+ +T Sbjct: 1078 HTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENT 1137 Query: 680 KYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLAELVGIVHRGT 856 + +LQQ+TY+L H++ ++ P P + A +A + GT Sbjct: 1138 ----------GNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGT 1186
>sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromosome III Length = 1040 Score = 102 bits (253), Expect = 2e-21 Identities = 86/293 (29%), Positives = 126/293 (43%), Gaps = 25/293 (8%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLKMA------------P 166 G+++QCV +N S R V+++ K+G GI+ ++ P Sbjct: 685 GIMSQCVLMKNVS----RPTPATCANIVLKLNMKMG----GINSRIVADKITNKYLVDQP 736 Query: 167 TMIVGIDTFHSKTGKR-----SVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLT 331 TM+VGID H + SV A V ++ Y + V K E L Sbjct: 737 TMVVGIDVTHPTQAEMRMNMPSVAAIVANVDLLPQSYGANVKVQKKCRESVVYLLDAIRE 796 Query: 332 AITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVV 511 I F + P R I+YRDGV + Q + E ++ I E+ P I Y+VV Sbjct: 797 RIITFYRHTKQKPARIIVYRDGVSEGQFSEVLREEIQSIRTACLAIAEDFR-PPITYIVV 855 Query: 512 KKRISVKFF--------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNV 667 +KR + F N PGT VD IV P ++FYL S +GT+ P Y+V Sbjct: 856 QKRHHARIFCKYQNDMVGKAKNVPPGTTVDTGIVSPEGFDFYLCSHYGVQGTSRPARYHV 915 Query: 668 LMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 826 L+D C +E+ Q ITY + H Y ++ +P PV+YAD +A Sbjct: 916 LLDE--CKFTADEI--------QSITYGMCHTYGRCTRSVSIPTPVYYADLVA 958
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Golgi ER protein 95 kDa) (GERp95) Length = 860 Score = 95.9 bits (237), Expect = 2e-19 Identities = 78/286 (27%), Positives = 122/286 (42%), Gaps = 18/286 (6%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADK------SVMQMFSKLGYDPWGISLKMAPTMIVGI 184 G+ TQCV +N + + K V + G P + P + +G Sbjct: 542 GMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGA 597 Query: 185 DTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQK 355 D H G K S+ A V S+ + ++Y + V + + E ++L + F + Sbjct: 598 DVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKS 657 Query: 356 FGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKF 535 P R I YRDGV + Q E A+ + K+ E + P I ++VV+KR + Sbjct: 658 TRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKEYQPGITFIVVQKRHHTRL 716 Query: 536 F---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 688 F K G P GT VD KI P ++FYL S +GT+ P++Y+VL D Sbjct: 717 FCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--- 772 Query: 689 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 826 + + LQ +TY L H Y ++ +P P +YA +A Sbjct: 773 -------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 811
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 Length = 997 Score = 95.5 bits (236), Expect = 2e-19 Identities = 67/244 (27%), Positives = 111/244 (45%), Gaps = 27/244 (11%) Frame = +2 Query: 164 PTMIVGIDTFHSKTGKRSVQASVFSISS----KFSQYMSFVNSPKGKNEFHENLGKNFLT 331 PT+I+G D H + G+ S + ++S + ++Y V++ + E ++L K Sbjct: 714 PTIIMGADVTHPQPGEDSSPSIAAVVASMDWPEINKYRGLVSAQAHREEIIQDLYKLVQD 773 Query: 332 A-------------ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIY 472 AF++ G +P R I YRDGV + Q E A+ K + Sbjct: 774 PQRGLVHSGLIREHFIAFRRATGQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQ 833 Query: 473 ENQNLPQIIYVVVKKRISVKFFKD----------GANPNPGTVVDEKIVKPNFYEFYLVS 622 EN +P++ +V+V+KR + F + N PGTVVD KI PN ++FYL S Sbjct: 834 ENY-VPRVTFVIVQKRHHTRLFPEQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNS 892 Query: 623 QRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVP 802 +GT+ P +Y+VL+D + LQ +T +L + Y ++ + P Sbjct: 893 HAGIQGTSRPAHYHVLLDE----------NGFTADQLQMLTNNLCYTYARCTKSVSIVPP 942 Query: 803 VHYA 814 +YA Sbjct: 943 AYYA 946
>sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3) (Argonaute-3) (Piwi/argonaute family protein meIF2C3) Length = 860 Score = 95.1 bits (235), Expect = 3e-19 Identities = 77/284 (27%), Positives = 125/284 (44%), Gaps = 16/284 (5%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQM--FSKLGYDPWGISLKMAPTMIVGIDTFH 196 G+ TQCV +N + + K +++ + + S+ P + +G D H Sbjct: 542 GMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTH 601 Query: 197 SKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTM 367 G K S+ A V S+ + S+Y + V + + E ++L + F + Sbjct: 602 PPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFK 661 Query: 368 PHRFIIYRDGVGDSQLYLTKKFETDAVMKM---VEKIYENQNLPQIIYVVVKKRISVKFF 538 P R I YRDGV + Q +E A+ + +EK Y+ P I Y+VV+KR + F Sbjct: 662 PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQ----PGITYIVVQKRHHTRLF 717 Query: 539 ------KDGANPN--PGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNK 694 + G + N GT VD I P ++FYL S +GT+ P++Y+VL D + Sbjct: 718 CADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNF--- 774 Query: 695 KTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 826 LQ +TY L H Y ++ +P P +YA +A Sbjct: 775 -------FTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVA 811
>sp|O77503|I2C2_RABIT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) Length = 840 Score = 95.1 bits (235), Expect = 3e-19 Identities = 78/286 (27%), Positives = 121/286 (42%), Gaps = 18/286 (6%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADK------SVMQMFSKLGYDPWGISLKMAPTMIVGI 184 G+ TQCV +N + + K V + G P + P + +G Sbjct: 522 GMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGA 577 Query: 185 DTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQK 355 D H G K S+ A V S+ + ++Y + V + + E ++L + F + Sbjct: 578 DVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKS 637 Query: 356 FGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKF 535 P R I YRDGV + Q E A+ + K+ E P I ++VV+KR + Sbjct: 638 TRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQKRHHTRL 696 Query: 536 F---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 688 F K G P GT VD KI P ++FYL S +GT+ P++Y+VL D Sbjct: 697 FCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--- 752 Query: 689 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 826 + + LQ +TY L H Y ++ +P P +YA +A Sbjct: 753 -------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 791
>sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Slicer protein) (PAZ Piwi domain protein) (PPD) Length = 851 Score = 95.1 bits (235), Expect = 3e-19 Identities = 78/286 (27%), Positives = 121/286 (42%), Gaps = 18/286 (6%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADK------SVMQMFSKLGYDPWGISLKMAPTMIVGI 184 G+ TQCV +N + + K V + G P + P + +G Sbjct: 533 GMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGA 588 Query: 185 DTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQK 355 D H G K S+ A V S+ + ++Y + V + + E ++L + F + Sbjct: 589 DVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKS 648 Query: 356 FGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKF 535 P R I YRDGV + Q E A+ + K+ E P I ++VV+KR + Sbjct: 649 TRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQKRHHTRL 707 Query: 536 F---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 688 F K G P GT VD KI P ++FYL S +GT+ P++Y+VL D Sbjct: 708 FCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--- 763 Query: 689 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 826 + + LQ +TY L H Y ++ +P P +YA +A Sbjct: 764 -------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 802
>sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation factor 2C 1 (eIF2C 1) (eIF-2C 1) (Argonaute-1) (Piwi/argonaute family protein meIF2C1) Length = 857 Score = 94.7 bits (234), Expect = 3e-19 Identities = 80/282 (28%), Positives = 124/282 (43%), Gaps = 14/282 (4%) Frame = +2 Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQM--FSKLGYDPWGISLKMAPTMIVGIDTFH 196 G+ TQCV +N + + K +++ + + ++ P + +G D H Sbjct: 539 GMATQCVQVKNAVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTH 598 Query: 197 SKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTM 367 G K S+ A V S+ + S+Y + V + + E E+L + F + Sbjct: 599 PPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFK 658 Query: 368 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF--- 538 P R I YRDGV + QL +E A+ K+ E P I Y+VV+KR + F Sbjct: 659 PTRIIFYRDGVPEGQLPQILHYELLAIRDACIKL-EKDYQPGITYIVVQKRHHTRLFCAD 717 Query: 539 ------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKT 700 K G P GT VD I P ++FYL S +GT+ P++Y VL D N+ T Sbjct: 718 KNERIGKSGNIP-AGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDD---NRFT 773 Query: 701 NEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 826 + LQ +TY L H Y ++ +P P +YA +A Sbjct: 774 AD-------ELQILTYQLCHTYVRCTRSVSIPAPAYYARLVA 808
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 119,471,304 Number of Sequences: 369166 Number of extensions: 2537624 Number of successful extensions: 5418 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 5229 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5383 length of database: 68,354,980 effective HSP length: 111 effective length of database: 47,849,395 effective search space used: 10287619925 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)