Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_J07
(820 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8UVX0|PIWI_BRARE Piwi protein 172 1e-42
sp|Q9VKM1|PIWI_DROME Piwi protein 159 9e-39
sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein 101 3e-21
sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromo... 100 4e-21
sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 96 2e-19
sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 95 2e-19
sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation fact... 94 3e-19
sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation fact... 94 3e-19
sp|O77503|I2C2_RABIT Eukaryotic translation initiation fact... 94 3e-19
sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation fact... 94 3e-19
>sp|Q8UVX0|PIWI_BRARE Piwi protein
Length = 858
Score = 172 bits (436), Expect = 1e-42
Identities = 98/268 (36%), Positives = 151/268 (56%), Gaps = 5/268 (1%)
Frame = +2
Query: 32 TQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLKMAPTMIVGIDTFH-SKTG 208
+QCV R S + TVA K +QM K+G + W + + + MIVGID +H + G
Sbjct: 580 SQCVVSRTISK--PQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAG 637
Query: 209 KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIY 388
KRS+ A V S++ S++ S E + L + A+ A+ + ++P R I+Y
Sbjct: 638 KRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVY 697
Query: 389 RDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFFK--DG--ANP 556
RDGVGD L +E +M+ ++ + ++ P++ VVVKKRIS +FF DG ANP
Sbjct: 698 RDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANP 756
Query: 557 NPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQ 736
PGTV+D ++ +P +Y+F++VSQ G +PT+YNV+ D S + P +Q
Sbjct: 757 PPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQ 806
Query: 737 QITYSLTHLYFNWMGTIRVPVPVHYADR 820
++TY L H+Y+NW G +RVP P YA +
Sbjct: 807 RLTYKLCHMYYNWQGIVRVPAPCQYAHK 834
>sp|Q9VKM1|PIWI_DROME Piwi protein
Length = 843
Score = 159 bits (402), Expect = 9e-39
Identities = 93/267 (34%), Positives = 140/267 (52%), Gaps = 4/267 (1%)
Frame = +2
Query: 32 TQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLKMAPTMIVGIDTFHS-KTG 208
TQ VT + NR ++A K +Q+ KLGY PW I L ++ M +G D S +
Sbjct: 566 TQVVTLKTTKNRS---LMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDR 622
Query: 209 KRSVQASVFSIS-SKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFII 385
KR+ A + S+ + S Y S V + L A+ +Q + +P R +
Sbjct: 623 KRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVF 682
Query: 386 YRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNL--PQIIYVVVKKRISVKFFKDGANPN 559
YRDGV L +FE +++ ++ Y L PQ+ Y+VV + ++ +FF +G NP
Sbjct: 683 YRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPP 742
Query: 560 PGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQ 739
PGT+VD+ I P Y+FYLVSQ+ +GT SPT+YNVL + ++P +Q+
Sbjct: 743 PGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSS----------MGLSPEKMQK 792
Query: 740 ITYSLTHLYFNWMGTIRVPVPVHYADR 820
+TY + HLY+NW GT RVP YA +
Sbjct: 793 LTYKMCHLYYNWSGTTRVPAVCQYAKK 819
>sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein
Length = 1214
Score = 101 bits (251), Expect = 3e-21
Identities = 81/285 (28%), Positives = 129/285 (45%), Gaps = 21/285 (7%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLK---------MAPTMI 175
G+LTQC+ Q + + + ++++ SKL GI+ K M TM
Sbjct: 909 GILTQCIKQFTVERKCNNQ---TIGNILLKINSKLN----GINHKIKDDPRLPMMKNTMY 961
Query: 176 VGIDTFHSKTGKRSVQASVFSISSKFSQYMSFVNSP----KGKNEFHENLGKNFLTAITA 343
+G D H +R + SV +++ Y + N +G E E++ L +
Sbjct: 962 IGADVTHPSPDQREIP-SVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSITLEHLRV 1020
Query: 344 FQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRI 523
+++ P I YRDGV D Q K E + + +K+ P+I V+V KR
Sbjct: 1021 YKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCK---PKICCVIVVKRH 1077
Query: 524 SVKFFKDGA--------NPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDT 679
+FF G N +PGTVVD IV PN +F++VS + +GTA PT YNV+ +T
Sbjct: 1078 HTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENT 1137
Query: 680 KYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 814
+ +LQQ+TY+L H++ ++ P P + A
Sbjct: 1138 ----------GNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLA 1172
>sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromosome III
Length = 1040
Score = 100 bits (250), Expect = 4e-21
Identities = 85/290 (29%), Positives = 124/290 (42%), Gaps = 25/290 (8%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGISLKMA------------P 166
G+++QCV +N S R V+++ K+G GI+ ++ P
Sbjct: 685 GIMSQCVLMKNVS----RPTPATCANIVLKLNMKMG----GINSRIVADKITNKYLVDQP 736
Query: 167 TMIVGIDTFHSKTGKR-----SVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLT 331
TM+VGID H + SV A V ++ Y + V K E L
Sbjct: 737 TMVVGIDVTHPTQAEMRMNMPSVAAIVANVDLLPQSYGANVKVQKKCRESVVYLLDAIRE 796
Query: 332 AITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVV 511
I F + P R I+YRDGV + Q + E ++ I E+ P I Y+VV
Sbjct: 797 RIITFYRHTKQKPARIIVYRDGVSEGQFSEVLREEIQSIRTACLAIAEDFR-PPITYIVV 855
Query: 512 KKRISVKFF--------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNV 667
+KR + F N PGT VD IV P ++FYL S +GT+ P Y+V
Sbjct: 856 QKRHHARIFCKYQNDMVGKAKNVPPGTTVDTGIVSPEGFDFYLCSHYGVQGTSRPARYHV 915
Query: 668 LMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYAD 817
L+D C +E+ Q ITY + H Y ++ +P PV+YAD
Sbjct: 916 LLDE--CKFTADEI--------QSITYGMCHTYGRCTRSVSIPTPVYYAD 955
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880
Length = 997
Score = 95.5 bits (236), Expect = 2e-19
Identities = 67/244 (27%), Positives = 111/244 (45%), Gaps = 27/244 (11%)
Frame = +2
Query: 164 PTMIVGIDTFHSKTGKRSVQASVFSISS----KFSQYMSFVNSPKGKNEFHENLGKNFLT 331
PT+I+G D H + G+ S + ++S + ++Y V++ + E ++L K
Sbjct: 714 PTIIMGADVTHPQPGEDSSPSIAAVVASMDWPEINKYRGLVSAQAHREEIIQDLYKLVQD 773
Query: 332 A-------------ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIY 472
AF++ G +P R I YRDGV + Q E A+ K +
Sbjct: 774 PQRGLVHSGLIREHFIAFRRATGQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQ 833
Query: 473 ENQNLPQIIYVVVKKRISVKFFKD----------GANPNPGTVVDEKIVKPNFYEFYLVS 622
EN +P++ +V+V+KR + F + N PGTVVD KI PN ++FYL S
Sbjct: 834 ENY-VPRVTFVIVQKRHHTRLFPEQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNS 892
Query: 623 QRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVP 802
+GT+ P +Y+VL+D + LQ +T +L + Y ++ + P
Sbjct: 893 HAGIQGTSRPAHYHVLLDE----------NGFTADQLQMLTNNLCYTYARCTKSVSIVPP 942
Query: 803 VHYA 814
+YA
Sbjct: 943 AYYA 946
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Golgi ER protein 95 kDa) (GERp95)
Length = 860
Score = 95.1 bits (235), Expect = 2e-19
Identities = 77/282 (27%), Positives = 120/282 (42%), Gaps = 18/282 (6%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADK------SVMQMFSKLGYDPWGISLKMAPTMIVGI 184
G+ TQCV +N + + K V + G P + P + +G
Sbjct: 542 GMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGA 597
Query: 185 DTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQK 355
D H G K S+ A V S+ + ++Y + V + + E ++L + F +
Sbjct: 598 DVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKS 657
Query: 356 FGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKF 535
P R I YRDGV + Q E A+ + K+ E + P I ++VV+KR +
Sbjct: 658 TRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKEYQPGITFIVVQKRHHTRL 716
Query: 536 F---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 688
F K G P GT VD KI P ++FYL S +GT+ P++Y+VL D
Sbjct: 717 FCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--- 772
Query: 689 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 814
+ + LQ +TY L H Y ++ +P P +YA
Sbjct: 773 -------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYA 807
>sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation factor 2C 1 (eIF2C 1) (eIF-2C 1)
(Argonaute-1) (Piwi/argonaute family protein meIF2C1)
Length = 857
Score = 94.4 bits (233), Expect = 3e-19
Identities = 79/278 (28%), Positives = 122/278 (43%), Gaps = 14/278 (5%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQM--FSKLGYDPWGISLKMAPTMIVGIDTFH 196
G+ TQCV +N + + K +++ + + ++ P + +G D H
Sbjct: 539 GMATQCVQVKNAVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTH 598
Query: 197 SKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTM 367
G K S+ A V S+ + S+Y + V + + E E+L + F +
Sbjct: 599 PPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFK 658
Query: 368 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF--- 538
P R I YRDGV + QL +E A+ K+ E P I Y+VV+KR + F
Sbjct: 659 PTRIIFYRDGVPEGQLPQILHYELLAIRDACIKL-EKDYQPGITYIVVQKRHHTRLFCAD 717
Query: 539 ------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKT 700
K G P GT VD I P ++FYL S +GT+ P++Y VL D N+ T
Sbjct: 718 KNERIGKSGNIP-AGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDD---NRFT 773
Query: 701 NEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 814
+ LQ +TY L H Y ++ +P P +YA
Sbjct: 774 AD-------ELQILTYQLCHTYVRCTRSVSIPAPAYYA 804
>sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3)
(Argonaute-3) (Piwi/argonaute family protein meIF2C3)
Length = 860
Score = 94.4 bits (233), Expect = 3e-19
Identities = 76/280 (27%), Positives = 123/280 (43%), Gaps = 16/280 (5%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADKSVMQM--FSKLGYDPWGISLKMAPTMIVGIDTFH 196
G+ TQCV +N + + K +++ + + S+ P + +G D H
Sbjct: 542 GMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTH 601
Query: 197 SKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTM 367
G K S+ A V S+ + S+Y + V + + E ++L + F +
Sbjct: 602 PPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFK 661
Query: 368 PHRFIIYRDGVGDSQLYLTKKFETDAVMKM---VEKIYENQNLPQIIYVVVKKRISVKFF 538
P R I YRDGV + Q +E A+ + +EK Y+ P I Y+VV+KR + F
Sbjct: 662 PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQ----PGITYIVVQKRHHTRLF 717
Query: 539 ------KDGANPN--PGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNK 694
+ G + N GT VD I P ++FYL S +GT+ P++Y+VL D +
Sbjct: 718 CADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNF--- 774
Query: 695 KTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 814
LQ +TY L H Y ++ +P P +YA
Sbjct: 775 -------FTADELQLLTYQLCHTYVRCTRSVSIPAPAYYA 807
>sp|O77503|I2C2_RABIT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2)
Length = 840
Score = 94.4 bits (233), Expect = 3e-19
Identities = 77/282 (27%), Positives = 119/282 (42%), Gaps = 18/282 (6%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADK------SVMQMFSKLGYDPWGISLKMAPTMIVGI 184
G+ TQCV +N + + K V + G P + P + +G
Sbjct: 522 GMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGA 577
Query: 185 DTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQK 355
D H G K S+ A V S+ + ++Y + V + + E ++L + F +
Sbjct: 578 DVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKS 637
Query: 356 FGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKF 535
P R I YRDGV + Q E A+ + K+ E P I ++VV+KR +
Sbjct: 638 TRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQKRHHTRL 696
Query: 536 F---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 688
F K G P GT VD KI P ++FYL S +GT+ P++Y+VL D
Sbjct: 697 FCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--- 752
Query: 689 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 814
+ + LQ +TY L H Y ++ +P P +YA
Sbjct: 753 -------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYA 787
>sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Slicer protein) (PAZ Piwi domain protein)
(PPD)
Length = 851
Score = 94.4 bits (233), Expect = 3e-19
Identities = 77/282 (27%), Positives = 119/282 (42%), Gaps = 18/282 (6%)
Frame = +2
Query: 23 GLLTQCVTQRNGSNRDDRRRKTVADK------SVMQMFSKLGYDPWGISLKMAPTMIVGI 184
G+ TQCV +N + + K V + G P + P + +G
Sbjct: 533 GMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGA 588
Query: 185 DTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQK 355
D H G K S+ A V S+ + ++Y + V + + E ++L + F +
Sbjct: 589 DVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKS 648
Query: 356 FGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKF 535
P R I YRDGV + Q E A+ + K+ E P I ++VV+KR +
Sbjct: 649 TRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQKRHHTRL 707
Query: 536 F---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 688
F K G P GT VD KI P ++FYL S +GT+ P++Y+VL D
Sbjct: 708 FCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--- 763
Query: 689 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 814
+ + LQ +TY L H Y ++ +P P +YA
Sbjct: 764 -------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYA 798
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,605,284
Number of Sequences: 369166
Number of extensions: 2040086
Number of successful extensions: 4320
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4285
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7859674995
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)