Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_M05
(625 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8UVX0|PIWI_BRARE Piwi protein 137 3e-32
sp|Q9VKM1|PIWI_DROME Piwi protein 134 2e-31
sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein 88 2e-17
sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromo... 87 3e-17
sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 85 2e-16
sp|O04379|AGO1_ARATH Argonaute protein 81 2e-15
sp|Q9XGW1|PINH_ARATH PINHEAD protein (ZWILLE protein) 81 2e-15
sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 80 5e-15
sp|O77503|I2C2_RABIT Eukaryotic translation initiation fact... 79 9e-15
sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation fact... 79 9e-15
>sp|Q8UVX0|PIWI_BRARE Piwi protein
Length = 858
Score = 137 bits (344), Expect = 3e-32
Identities = 75/186 (40%), Positives = 114/186 (61%), Gaps = 5/186 (2%)
Frame = +1
Query: 7 TMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFFK 186
++P R I+YRDGVGD L +E +M+ ++ + ++ P++ VVVKKRIS +FF
Sbjct: 689 SLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFA 747
Query: 187 --DG--ANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEV 354
DG ANP PGTV+D ++ +P +Y+F++VSQ G +PT+YNV+ D
Sbjct: 748 RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN---------- 797
Query: 355 SPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLAELVG-IVHRGTAPPTINERIR 531
S + P +Q++TY L H+Y+NW G +RVP P YA +LA LVG +H+ P +N +
Sbjct: 798 SGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHK---EPNMN--LD 852
Query: 532 ERLFYL 549
+ L+YL
Sbjct: 853 DFLYYL 858
>sp|Q9VKM1|PIWI_DROME Piwi protein
Length = 843
Score = 134 bits (337), Expect = 2e-31
Identities = 71/181 (39%), Positives = 104/181 (57%), Gaps = 2/181 (1%)
Frame = +1
Query: 10 MPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNL--PQIIYVVVKKRISVKFF 183
+P R + YRDGV L +FE +++ ++ Y L PQ+ Y+VV + ++ +FF
Sbjct: 676 LPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFF 735
Query: 184 KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPM 363
+G NP PGT+VD+ I P Y+FYLVSQ+ +GT SPT+YNVL + +
Sbjct: 736 LNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSS----------MGL 785
Query: 364 APSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLAELVGIVHRGTAPPTINERIRERLF 543
+P +Q++TY + HLY+NW GT RVP YA +LA LV GT +I + E+ F
Sbjct: 786 SPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLV-----GTNLHSIPQNALEKKF 840
Query: 544 Y 546
Y
Sbjct: 841 Y 841
>sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein
Length = 1214
Score = 87.8 bits (216), Expect = 2e-17
Identities = 57/171 (33%), Positives = 83/171 (48%), Gaps = 8/171 (4%)
Frame = +1
Query: 13 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFFKDG 192
P I YRDGV D Q K E + + +K+ P+I V+V KR +FF G
Sbjct: 1029 PDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCK---PKICCVIVVKRHHTRFFPSG 1085
Query: 193 A--------NPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTN 348
N +PGTVVD IV PN +F++VS + +GTA PT YNV+ +T
Sbjct: 1086 DVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENT-------- 1137
Query: 349 EVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLAELVGIVHRGT 501
+ +LQQ+TY+L H++ ++ P P + A +A + GT
Sbjct: 1138 --GNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRVYLTGT 1186
>sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromosome III
Length = 1040
Score = 87.0 bits (214), Expect = 3e-17
Identities = 55/161 (34%), Positives = 78/161 (48%), Gaps = 8/161 (4%)
Frame = +1
Query: 13 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF--- 183
P R I+YRDGV + Q + E ++ I E+ P I Y+VV+KR + F
Sbjct: 809 PARIIVYRDGVSEGQFSEVLREEIQSIRTACLAIAEDFR-PPITYIVVQKRHHARIFCKY 867
Query: 184 -----KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTN 348
N PGT VD IV P ++FYL S +GT+ P Y+VL+D C +
Sbjct: 868 QNDMVGKAKNVPPGTTVDTGIVSPEGFDFYLCSHYGVQGTSRPARYHVLLDE--CKFTAD 925
Query: 349 EVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 471
E+ Q ITY + H Y ++ +P PV+YAD +A
Sbjct: 926 EI--------QSITYGMCHTYGRCTRSVSIPTPVYYADLVA 958
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880
Length = 997
Score = 84.7 bits (208), Expect = 2e-16
Identities = 51/162 (31%), Positives = 78/162 (48%), Gaps = 10/162 (6%)
Frame = +1
Query: 4 GTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF 183
G +P R I YRDGV + Q E A+ K + EN +P++ +V+V+KR + F
Sbjct: 796 GQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQENY-VPRVTFVIVQKRHHTRLF 854
Query: 184 KD----------GANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 333
+ N PGTVVD KI PN ++FYL S +GT+ P +Y+VL+D
Sbjct: 855 PEQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNSHAGIQGTSRPAHYHVLLDE--- 911
Query: 334 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 459
+ LQ +T +L + Y ++ + P +YA
Sbjct: 912 -------NGFTADQLQMLTNNLCYTYARCTKSVSIVPPAYYA 946
>sp|O04379|AGO1_ARATH Argonaute protein
Length = 1048
Score = 80.9 bits (198), Expect = 2e-15
Identities = 52/162 (32%), Positives = 74/162 (45%), Gaps = 10/162 (6%)
Frame = +1
Query: 4 GTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF 183
G P R I YRDGV + Q Y +E DA+ K + E P + +VVV+KR + F
Sbjct: 835 GHKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASL-EAGYQPPVTFVVVQKRHHTRLF 893
Query: 184 KDGANPN----------PGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 333
N PGTVVD KI P ++FYL S +GT+ P +Y+VL D
Sbjct: 894 AQNHNDRHSVDRSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDE--- 950
Query: 334 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 459
+ LQ +T +L + Y ++ + P +YA
Sbjct: 951 -------NNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYA 985
>sp|Q9XGW1|PINH_ARATH PINHEAD protein (ZWILLE protein)
Length = 988
Score = 80.9 bits (198), Expect = 2e-15
Identities = 50/162 (30%), Positives = 75/162 (46%), Gaps = 10/162 (6%)
Frame = +1
Query: 4 GTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF 183
G P R I YRDGV + Q Y +E DA+ K + N P + ++VV+KR + F
Sbjct: 784 GQKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEPNYQ-PPVTFIVVQKRHHTRLF 842
Query: 184 KDG----------ANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYC 333
+ N PGTVVD KI P ++FYL S +GT+ P +Y+VL D
Sbjct: 843 ANNHRDKNSTDRSGNILPGTVVDTKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDE--- 899
Query: 334 NKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 459
+ +Q +T +L + Y ++ + P +YA
Sbjct: 900 -------NNFTADGIQSLTNNLCYTYARCTRSVSIVPPAYYA 934
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Golgi ER protein 95 kDa) (GERp95)
Length = 860
Score = 79.7 bits (195), Expect = 5e-15
Identities = 53/162 (32%), Positives = 77/162 (47%), Gaps = 9/162 (5%)
Frame = +1
Query: 13 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF--- 183
P R I YRDGV + Q E A+ + K+ E + P I ++VV+KR + F
Sbjct: 662 PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKEYQPGITFIVVQKRHHTRLFCTD 720
Query: 184 ------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKT 345
K G P GT VD KI P ++FYL S +GT+ P++Y+VL D
Sbjct: 721 KNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD------- 772
Query: 346 NEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 471
+ + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 773 ---NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 811
>sp|O77503|I2C2_RABIT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2)
Length = 840
Score = 79.0 bits (193), Expect = 9e-15
Identities = 53/162 (32%), Positives = 76/162 (46%), Gaps = 9/162 (5%)
Frame = +1
Query: 13 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF--- 183
P R I YRDGV + Q E A+ + K+ E P I ++VV+KR + F
Sbjct: 642 PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQKRHHTRLFCTD 700
Query: 184 ------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKT 345
K G P GT VD KI P ++FYL S +GT+ P++Y+VL D
Sbjct: 701 KNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD------- 752
Query: 346 NEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 471
+ + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 753 ---NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 791
>sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Slicer protein) (PAZ Piwi domain protein)
(PPD)
Length = 851
Score = 79.0 bits (193), Expect = 9e-15
Identities = 53/162 (32%), Positives = 76/162 (46%), Gaps = 9/162 (5%)
Frame = +1
Query: 13 PHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF--- 183
P R I YRDGV + Q E A+ + K+ E P I ++VV+KR + F
Sbjct: 653 PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQKRHHTRLFCTD 711
Query: 184 ------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKT 345
K G P GT VD KI P ++FYL S +GT+ P++Y+VL D
Sbjct: 712 KNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD------- 763
Query: 346 NEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 471
+ + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 764 ---NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 802
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,262,487
Number of Sequences: 369166
Number of extensions: 1511282
Number of successful extensions: 3331
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3237
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3299
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)