Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_K18
(913 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8UVX0|PIWI_BRARE Piwi protein 177 5e-44
sp|Q9VKM1|PIWI_DROME Piwi protein 163 5e-40
sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein 99 1e-20
sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromo... 98 4e-20
sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 96 2e-19
sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation fact... 94 4e-19
sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 94 4e-19
sp|O77503|I2C2_RABIT Eukaryotic translation initiation fact... 94 7e-19
sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation fact... 94 7e-19
sp|Q9XGW1|PINH_ARATH PINHEAD protein (ZWILLE protein) 93 9e-19
>sp|Q8UVX0|PIWI_BRARE Piwi protein
Length = 858
Score = 177 bits (448), Expect = 5e-44
Identities = 98/256 (38%), Positives = 149/256 (58%), Gaps = 5/256 (1%)
Frame = +3
Query: 24 TVADKSVMQMFSKLGYDPWGISLKMAPTMIVGIDTFH-SKTGKRSVQASVFSISSKFSQY 200
TVA K +QM K+G + W + + + MIVGID +H + GKRS+ A V S++ S++
Sbjct: 596 TVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRW 655
Query: 201 MSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETD 380
S E + L + A+ A+ + ++P R I+YRDGVGD L +E
Sbjct: 656 FSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVP 715
Query: 381 AVMKMVEKIYENQNLPQIIYVVVKKRISVKFFK--DG--ANPNPGTVVDEKIVKPNFYEF 548
+M+ ++ + ++ P++ VVVKKRIS +FF DG ANP PGTV+D ++ +P +Y+F
Sbjct: 716 QIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTRPEWYDF 774
Query: 549 YLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIR 728
++VSQ G +PT+YNV+ D S + P +Q++TY L H+Y+NW G +R
Sbjct: 775 FIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQRLTYKLCHMYYNWQGIVR 824
Query: 729 VPVPVHYADRLAELVG 776
VP P YA +LA LVG
Sbjct: 825 VPAPCQYAHKLAFLVG 840
>sp|Q9VKM1|PIWI_DROME Piwi protein
Length = 843
Score = 163 bits (413), Expect = 5e-40
Identities = 92/255 (36%), Positives = 138/255 (54%), Gaps = 4/255 (1%)
Frame = +3
Query: 24 TVADKSVMQMFSKLGYDPWGISLKMAPTMIVGIDTFHS-KTGKRSVQASVFSIS-SKFSQ 197
++A K +Q+ KLGY PW I L ++ M +G D S + KR+ A + S+ + S
Sbjct: 581 SIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNST 640
Query: 198 YMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFET 377
Y S V + L A+ +Q + +P R + YRDGV L +FE
Sbjct: 641 YFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEV 700
Query: 378 DAVMKMVEKIYENQNL--PQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFY 551
+++ ++ Y L PQ+ Y+VV + ++ +FF +G NP PGT+VD+ I P Y+FY
Sbjct: 701 KDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFY 760
Query: 552 LVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRV 731
LVSQ+ +GT SPT+YNVL + ++P +Q++TY + HLY+NW GT RV
Sbjct: 761 LVSQQVRQGTVSPTSYNVLYSS----------MGLSPEKMQKLTYKMCHLYYNWSGTTRV 810
Query: 732 PVPVHYADRLAELVG 776
P YA +LA LVG
Sbjct: 811 PAVCQYAKKLATLVG 825
>sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein
Length = 1214
Score = 99.4 bits (246), Expect = 1e-20
Identities = 72/245 (29%), Positives = 112/245 (45%), Gaps = 12/245 (4%)
Frame = +3
Query: 96 MAPTMIVGIDTFHSKTGKRSVQASVFSISSKFSQYMSFVNSP----KGKNEFHENLGKNF 263
M TM +G D H +R + SV +++ Y + N +G E E++
Sbjct: 956 MKNTMYIGADVTHPSPDQREIP-SVVGVAASHDPYGASYNMQYRLQRGALEEIEDMFSIT 1014
Query: 264 LTAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYV 443
L + +++ P I YRDGV D Q K E + + +K+ P+I V
Sbjct: 1015 LEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKVGCK---PKICCV 1071
Query: 444 VVKKRISVKFFKDGA--------NPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNY 599
+V KR +FF G N +PGTVVD IV PN +F++VS + +GTA PT Y
Sbjct: 1072 IVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRY 1131
Query: 600 NVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLAELVGI 779
NV+ +T + +LQQ+TY+L H++ ++ P P + A +A +
Sbjct: 1132 NVIENT----------GNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRV 1181
Query: 780 VHRGT 794
GT
Sbjct: 1182 YLTGT 1186
>sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromosome III
Length = 1040
Score = 97.8 bits (242), Expect = 4e-20
Identities = 74/234 (31%), Positives = 103/234 (44%), Gaps = 13/234 (5%)
Frame = +3
Query: 102 PTMIVGIDTFHSKTGKR-----SVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFL 266
PTM+VGID H + SV A V ++ Y + V K E L
Sbjct: 736 PTMVVGIDVTHPTQAEMRMNMPSVAAIVANVDLLPQSYGANVKVQKKCRESVVYLLDAIR 795
Query: 267 TAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVV 446
I F + P R I+YRDGV + Q + E ++ I E+ P I Y+V
Sbjct: 796 ERIITFYRHTKQKPARIIVYRDGVSEGQFSEVLREEIQSIRTACLAIAEDFR-PPITYIV 854
Query: 447 VKKRISVKFF--------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYN 602
V+KR + F N PGT VD IV P ++FYL S +GT+ P Y+
Sbjct: 855 VQKRHHARIFCKYQNDMVGKAKNVPPGTTVDTGIVSPEGFDFYLCSHYGVQGTSRPARYH 914
Query: 603 VLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 764
VL+D C +E+ Q ITY + H Y ++ +P PV+YAD +A
Sbjct: 915 VLLDE--CKFTADEI--------QSITYGMCHTYGRCTRSVSIPTPVYYADLVA 958
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880
Length = 997
Score = 95.5 bits (236), Expect = 2e-19
Identities = 67/244 (27%), Positives = 111/244 (45%), Gaps = 27/244 (11%)
Frame = +3
Query: 102 PTMIVGIDTFHSKTGKRSVQASVFSISS----KFSQYMSFVNSPKGKNEFHENLGKNFLT 269
PT+I+G D H + G+ S + ++S + ++Y V++ + E ++L K
Sbjct: 714 PTIIMGADVTHPQPGEDSSPSIAAVVASMDWPEINKYRGLVSAQAHREEIIQDLYKLVQD 773
Query: 270 A-------------ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIY 410
AF++ G +P R I YRDGV + Q E A+ K +
Sbjct: 774 PQRGLVHSGLIREHFIAFRRATGQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQ 833
Query: 411 ENQNLPQIIYVVVKKRISVKFFKD----------GANPNPGTVVDEKIVKPNFYEFYLVS 560
EN +P++ +V+V+KR + F + N PGTVVD KI PN ++FYL S
Sbjct: 834 ENY-VPRVTFVIVQKRHHTRLFPEQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNS 892
Query: 561 QRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVP 740
+GT+ P +Y+VL+D + LQ +T +L + Y ++ + P
Sbjct: 893 HAGIQGTSRPAHYHVLLDE----------NGFTADQLQMLTNNLCYTYARCTKSVSIVPP 942
Query: 741 VHYA 752
+YA
Sbjct: 943 AYYA 946
>sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3)
(Argonaute-3) (Piwi/argonaute family protein meIF2C3)
Length = 860
Score = 94.4 bits (233), Expect = 4e-19
Identities = 70/240 (29%), Positives = 109/240 (45%), Gaps = 14/240 (5%)
Frame = +3
Query: 87 SLKMAPTMIVGIDTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGK 257
S+ P + +G D H G K S+ A V S+ + S+Y + V + + E ++L
Sbjct: 586 SVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLAS 645
Query: 258 NFLTAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKM---VEKIYENQNLP 428
+ F + P R I YRDGV + Q +E A+ + +EK Y+ P
Sbjct: 646 MVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQ----P 701
Query: 429 QIIYVVVKKRISVKFF------KDGANPN--PGTVVDEKIVKPNFYEFYLVSQRTTKGTA 584
I Y+VV+KR + F + G + N GT VD I P ++FYL S +GT+
Sbjct: 702 GITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTS 761
Query: 585 SPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 764
P++Y+VL D + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 762 RPSHYHVLWDDNF----------FTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVA 811
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Golgi ER protein 95 kDa) (GERp95)
Length = 860
Score = 94.4 bits (233), Expect = 4e-19
Identities = 68/233 (29%), Positives = 106/233 (45%), Gaps = 12/233 (5%)
Frame = +3
Query: 102 PTMIVGIDTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTA 272
P + +G D H G K S+ A V S+ + ++Y + V + + E ++L
Sbjct: 591 PVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVREL 650
Query: 273 ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVK 452
+ F + P R I YRDGV + Q E A+ + K+ E + P I ++VV+
Sbjct: 651 LIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKEYQPGITFIVVQ 709
Query: 453 KRISVKFF---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNV 605
KR + F K G P GT VD KI P ++FYL S +GT+ P++Y+V
Sbjct: 710 KRHHTRLFCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHV 768
Query: 606 LMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 764
L D + + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 769 LWDD----------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 811
>sp|O77503|I2C2_RABIT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2)
Length = 840
Score = 93.6 bits (231), Expect = 7e-19
Identities = 68/233 (29%), Positives = 105/233 (45%), Gaps = 12/233 (5%)
Frame = +3
Query: 102 PTMIVGIDTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTA 272
P + +G D H G K S+ A V S+ + ++Y + V + + E ++L
Sbjct: 571 PVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVREL 630
Query: 273 ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVK 452
+ F + P R I YRDGV + Q E A+ + K+ E P I ++VV+
Sbjct: 631 LIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQ 689
Query: 453 KRISVKFF---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNV 605
KR + F K G P GT VD KI P ++FYL S +GT+ P++Y+V
Sbjct: 690 KRHHTRLFCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHV 748
Query: 606 LMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 764
L D + + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 749 LWDD----------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 791
>sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Slicer protein) (PAZ Piwi domain protein)
(PPD)
Length = 851
Score = 93.6 bits (231), Expect = 7e-19
Identities = 68/233 (29%), Positives = 105/233 (45%), Gaps = 12/233 (5%)
Frame = +3
Query: 102 PTMIVGIDTFHSKTG---KRSVQASVFSISSKFSQYMSFVNSPKGKNEFHENLGKNFLTA 272
P + +G D H G K S+ A V S+ + ++Y + V + + E ++L
Sbjct: 582 PVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVREL 641
Query: 273 ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVK 452
+ F + P R I YRDGV + Q E A+ + K+ E P I ++VV+
Sbjct: 642 LIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKL-EKDYQPGITFIVVQ 700
Query: 453 KRISVKFF---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNV 605
KR + F K G P GT VD KI P ++FYL S +GT+ P++Y+V
Sbjct: 701 KRHHTRLFCTDKNERVGKSGNIP-AGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHV 759
Query: 606 LMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYADRLA 764
L D + + LQ +TY L H Y ++ +P P +YA +A
Sbjct: 760 LWDD----------NRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVA 802
>sp|Q9XGW1|PINH_ARATH PINHEAD protein (ZWILLE protein)
Length = 988
Score = 93.2 bits (230), Expect = 9e-19
Identities = 70/269 (26%), Positives = 117/269 (43%), Gaps = 27/269 (10%)
Frame = +3
Query: 102 PTMIVGIDTFHSKTGKRSVQASVFSISSK----FSQYMSFVNSPKGKNEFHENLGKNFLT 269
PT+I G D H + G+ S + ++S+ ++Y V + + E ++L K +
Sbjct: 702 PTIIFGADVTHPENGEESSPSIAAVVASQDWPEVTKYAGLVCAQAHRQELIQDLYKTWQD 761
Query: 270 AIT-------------AFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIY 410
+ +F++ G P R I YRDGV + Q Y +E DA+ K +
Sbjct: 762 PVRGTVSGGMIRDLLISFRKATGQKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASLE 821
Query: 411 ENQNLPQIIYVVVKKRISVKFFKDG----------ANPNPGTVVDEKIVKPNFYEFYLVS 560
N P + ++VV+KR + F + N PGTVVD KI P ++FYL S
Sbjct: 822 PNYQ-PPVTFIVVQKRHHTRLFANNHRDKNSTDRSGNILPGTVVDTKICHPTEFDFYLCS 880
Query: 561 QRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVP 740
+GT+ P +Y+VL D + +Q +T +L + Y ++ + P
Sbjct: 881 HAGIQGTSRPAHYHVLWDE----------NNFTADGIQSLTNNLCYTYARCTRSVSIVPP 930
Query: 741 VHYADRLAELVGIVHRGTAPPSLMRELGN 827
+Y A L R P +M++ G+
Sbjct: 931 AYY----AHLAAFRARFYLEPEIMQDNGS 955
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 111,072,534
Number of Sequences: 369166
Number of extensions: 2352478
Number of successful extensions: 5110
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4932
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5074
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9270587090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)