Planaria EST Database


DrC_00930

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00930
         (493 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P35479|CPI1_PIG  Leukocyte cysteine proteinase inhibitor ...    79   6e-15
sp|P01040|CYTA_HUMAN  Cystatin A (Stefin A) (Cystatin AS)          76   5e-14
sp|Q28988|CYTA1_PIG  Cystatin A1 (Stefin A1)                       73   4e-13
sp|Q8I030|CYTB_PANTR  Cystatin B (Stefin B)                        72   9e-13
sp|Q28986|CYTA5_PIG  Cystatin A5 (Stefin A5)                       72   9e-13
sp|Q862Z5|CYTB_MACFU  Cystatin B (Stefin B)                        71   1e-12
sp|Q28987|CYTA8_PIG  Cystatin A8 (Stefin A8)                       71   1e-12
sp|P60576|CYTB_PONPY  Cystatin B (Stefin B) >gi|45476793|sp|...    71   2e-12
sp|P01041|CYTB_RAT  Cystatin B (Liver thiol proteinase inhib...    70   3e-12
sp|P80416|CYTA_BOVIN  Cystatin A (Stefin A) (Cystatin AS)          69   8e-12
>sp|P35479|CPI1_PIG Leukocyte cysteine proteinase inhibitor 1 (PLCPI) (Stefin D1)
          Length = 103

 Score = 79.0 bits (193), Expect = 6e-15
 Identities = 45/98 (45%), Positives = 60/98 (61%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           ML GG    R  + E + I   VKPQ+E++   +   F+ +  RSQVVAGTNY+ KVH+G
Sbjct: 6   MLAGGLTEPRPATPEIQEIANKVKPQLEEKTNKTYEKFEAIIYRSQVVAGTNYYIKVHVG 65

Query: 241 DGKHIHIRVYQPLPGQGD-LALHGLQ-EKLKEDVLEYF 348
              ++HIRV+Q LP Q D L L G Q +K K+D L  F
Sbjct: 66  GNNYVHIRVFQSLPHQEDPLKLIGYQVDKTKDDELTGF 103
>sp|P01040|CYTA_HUMAN Cystatin A (Stefin A) (Cystatin AS)
          Length = 98

 Score = 75.9 bits (185), Expect = 5e-14
 Identities = 40/98 (40%), Positives = 62/98 (63%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           M+ GG    +  + E + I+  VKPQ+E++   +    + +Q ++QVVAGTNY+ KV  G
Sbjct: 1   MIPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAG 60

Query: 241 DGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           D K++H++V++ LPGQ  DL L G Q +K K+D L  F
Sbjct: 61  DNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF 98
>sp|Q28988|CYTA1_PIG Cystatin A1 (Stefin A1)
          Length = 103

 Score = 72.8 bits (177), Expect = 4e-13
 Identities = 39/100 (39%), Positives = 61/100 (61%), Gaps = 2/100 (2%)
 Frame = +1

Query: 55  NRMLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVH 234
           ++M+ GG    R  + E + I  TVK Q+E++   +   F+ ++ +SQVVAG NY+ KVH
Sbjct: 4   DKMMPGGLTEARPATPEIQEIATTVKSQLEEKTNKTYEKFEAVEYKSQVVAGINYYIKVH 63

Query: 235 IGDGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           +G   ++HI+V++ LP Q   L L G Q +K K+D L  F
Sbjct: 64  VGGNSYVHIKVFKSLPYQNKPLELSGYQVDKTKDDELTGF 103
>sp|Q8I030|CYTB_PANTR Cystatin B (Stefin B)
          Length = 98

 Score = 71.6 bits (174), Expect = 9e-13
 Identities = 39/98 (39%), Positives = 55/98 (56%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           M+CG     +  + E + I   V+ Q+E++       F  +  +SQVVAGTNYF KVH+G
Sbjct: 1   MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVG 60

Query: 241 DGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           D + +H+RV+Q LP +   L L   Q  K K D L YF
Sbjct: 61  DEEFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF 98
>sp|Q28986|CYTA5_PIG Cystatin A5 (Stefin A5)
          Length = 103

 Score = 71.6 bits (174), Expect = 9e-13
 Identities = 42/98 (42%), Positives = 58/98 (59%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           ML GG    R  + E + I   VKPQ+E++   +   F+ +  RSQVVAGTNY+ KVH+G
Sbjct: 6   MLAGGLTEPRPATPEIQEIANKVKPQLEEKTKKTYEKFEAIIYRSQVVAGTNYYIKVHVG 65

Query: 241 DGKHIHIRVYQPLPGQGD-LALHGLQ-EKLKEDVLEYF 348
              ++HI V+Q LP   + L L G Q +K K+D L  F
Sbjct: 66  GNSYVHIIVFQNLPQLNEPLKLIGYQVDKTKDDELTSF 103
>sp|Q862Z5|CYTB_MACFU Cystatin B (Stefin B)
          Length = 98

 Score = 71.2 bits (173), Expect = 1e-12
 Identities = 39/98 (39%), Positives = 55/98 (56%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           M+CG    V+  + E + I   V+ Q+E++       F  +  +SQVVAGTNYF KVH+G
Sbjct: 1   MMCGAPSAVQPATAETQDIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVG 60

Query: 241 DGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           D   +H+RV++ LP +   L L   Q  K K D L YF
Sbjct: 61  DEDFVHLRVFKSLPHENKPLTLSDYQTNKAKHDELSYF 98
>sp|Q28987|CYTA8_PIG Cystatin A8 (Stefin A8)
          Length = 103

 Score = 71.2 bits (173), Expect = 1e-12
 Identities = 42/98 (42%), Positives = 58/98 (59%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           ML GG    R  + E + I   VKPQ+E++   +   F+ +  RSQVVAGTNY+ KVH+G
Sbjct: 6   MLAGGLTEPRPATPEIQEIANKVKPQLEEKTKKTYEKFEAIIYRSQVVAGTNYYIKVHVG 65

Query: 241 DGKHIHIRVYQPLPGQGD-LALHGLQ-EKLKEDVLEYF 348
              ++HI V+Q LP   + L L G Q +K K+D L  F
Sbjct: 66  GNSYVHIIVFQNLPQLNEPLKLIGYQVDKTKDDELTGF 103
>sp|P60576|CYTB_PONPY Cystatin B (Stefin B)
 sp|P60575|CYTB_PANPA Cystatin B (Stefin B)
 sp|Q76LA0|CYTB_GORGO Cystatin B (Stefin B)
 sp|P04080|CYTB_HUMAN Cystatin B (Liver thiol proteinase inhibitor) (CPI-B) (Stefin B)
          Length = 98

 Score = 70.9 bits (172), Expect = 2e-12
 Identities = 39/98 (39%), Positives = 54/98 (55%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           M+CG     +  + E + I   V+ Q+E++       F  +  +SQVVAGTNYF KVH+G
Sbjct: 1   MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVG 60

Query: 241 DGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           D   +H+RV+Q LP +   L L   Q  K K D L YF
Sbjct: 61  DEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF 98
>sp|P01041|CYTB_RAT Cystatin B (Liver thiol proteinase inhibitor) (Stefin B) (Cystatin
           beta)
          Length = 98

 Score = 69.7 bits (169), Expect = 3e-12
 Identities = 38/98 (38%), Positives = 54/98 (55%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           M+CG        + E + I   VK Q+E++       F  +  R QVVAGTN+F KV +G
Sbjct: 1   MMCGAPSATMPATTETQEIADKVKSQLEEKANQKFDVFKAISFRRQVVAGTNFFIKVDVG 60

Query: 241 DGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           + K +H+RV++PLP +   L L   Q +K K D L YF
Sbjct: 61  EEKCVHLRVFEPLPHENKPLTLSSYQTDKEKHDELTYF 98
>sp|P80416|CYTA_BOVIN Cystatin A (Stefin A) (Cystatin AS)
          Length = 98

 Score = 68.6 bits (166), Expect = 8e-12
 Identities = 38/98 (38%), Positives = 58/98 (59%), Gaps = 2/98 (2%)
 Frame = +1

Query: 61  MLCGGTGNVRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIG 240
           M+ GG    +  + E + I   VKPQ+E++   +   F  ++ +SQVVAG NY+ K+  G
Sbjct: 1   MIPGGLTEAKPATIEIQEIANMVKPQLEEKTNETYEEFTAIEYKSQVVAGINYYIKIQTG 60

Query: 241 DGKHIHIRVYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 348
           D ++IHI+V++ LP Q   L L G Q +K K+D L  F
Sbjct: 61  DNRYIHIKVFKSLPQQSHSLILTGYQVDKTKDDELAGF 98
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,019,141
Number of Sequences: 369166
Number of extensions: 1037086
Number of successful extensions: 2358
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2317
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2355
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3020232610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00930

  1. Dr_sW_023_H20
  2. Dr_sW_007_F16
  3. Dr_sW_009_P17
  4. Dr_sW_013_A18