Planarian EST Database


Dr_sW_007_F16

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_007_F16
         (370 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P35479|CPI1_PIG  Leukocyte cysteine proteinase inhibitor ...    73   2e-13
sp|P01040|CYTA_HUMAN  Cystatin A (Stefin A) (Cystatin AS)          70   1e-12
sp|Q28988|CYTA1_PIG  Cystatin A1 (Stefin A1)                       67   2e-11
sp|Q28986|CYTA5_PIG  Cystatin A5 (Stefin A5)                       65   4e-11
sp|Q28987|CYTA8_PIG  Cystatin A8 (Stefin A8)                       65   5e-11
sp|Q8I030|CYTB_PANTR  Cystatin B (Stefin B)                        65   6e-11
sp|P80416|CYTA_BOVIN  Cystatin A (Stefin A) (Cystatin AS)          64   8e-11
sp|P60576|CYTB_PONPY  Cystatin B (Stefin B) >gi|45476793|sp|...    64   1e-10
sp|Q862Z5|CYTB_MACFU  Cystatin B (Stefin B)                        64   1e-10
sp|P01041|CYTB_RAT  Cystatin B (Liver thiol proteinase inhib...    64   1e-10
>sp|P35479|CPI1_PIG Leukocyte cysteine proteinase inhibitor 1 (PLCPI) (Stefin D1)
          Length = 103

 Score = 72.8 bits (177), Expect = 2e-13
 Identities = 41/89 (46%), Positives = 56/89 (62%), Gaps = 2/89 (2%)
 Frame = +2

Query: 14  RTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRV 193
           R  + E + I   VKPQ+E++   +   F+ +  RSQVVAGTNY+ KVH+G   ++HIRV
Sbjct: 15  RPATPEIQEIANKVKPQLEEKTNKTYEKFEAIIYRSQVVAGTNYYIKVHVGGNNYVHIRV 74

Query: 194 YQPLPGQGD-LALHGLQ-EKLKEDVLEYF 274
           +Q LP Q D L L G Q +K K+D L  F
Sbjct: 75  FQSLPHQEDPLKLIGYQVDKTKDDELTGF 103
>sp|P01040|CYTA_HUMAN Cystatin A (Stefin A) (Cystatin AS)
          Length = 98

 Score = 70.5 bits (171), Expect = 1e-12
 Identities = 39/88 (44%), Positives = 58/88 (65%), Gaps = 2/88 (2%)
 Frame = +2

Query: 17  TPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRVY 196
           TP  +E  I+  VKPQ+E++   +    + +Q ++QVVAGTNY+ KV  GD K++H++V+
Sbjct: 13  TPEIQE--IVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVF 70

Query: 197 QPLPGQG-DLALHGLQ-EKLKEDVLEYF 274
           + LPGQ  DL L G Q +K K+D L  F
Sbjct: 71  KSLPGQNEDLVLTGYQVDKNKDDELTGF 98
>sp|Q28988|CYTA1_PIG Cystatin A1 (Stefin A1)
          Length = 103

 Score = 66.6 bits (161), Expect = 2e-11
 Identities = 36/89 (40%), Positives = 55/89 (61%), Gaps = 2/89 (2%)
 Frame = +2

Query: 14  RTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRV 193
           R  + E + I  TVK Q+E++   +   F+ ++ +SQVVAG NY+ KVH+G   ++HI+V
Sbjct: 15  RPATPEIQEIATTVKSQLEEKTNKTYEKFEAVEYKSQVVAGINYYIKVHVGGNSYVHIKV 74

Query: 194 YQPLPGQG-DLALHGLQ-EKLKEDVLEYF 274
           ++ LP Q   L L G Q +K K+D L  F
Sbjct: 75  FKSLPYQNKPLELSGYQVDKTKDDELTGF 103
>sp|Q28986|CYTA5_PIG Cystatin A5 (Stefin A5)
          Length = 103

 Score = 65.5 bits (158), Expect = 4e-11
 Identities = 38/89 (42%), Positives = 54/89 (60%), Gaps = 2/89 (2%)
 Frame = +2

Query: 14  RTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRV 193
           R  + E + I   VKPQ+E++   +   F+ +  RSQVVAGTNY+ KVH+G   ++HI V
Sbjct: 15  RPATPEIQEIANKVKPQLEEKTKKTYEKFEAIIYRSQVVAGTNYYIKVHVGGNSYVHIIV 74

Query: 194 YQPLPGQGD-LALHGLQ-EKLKEDVLEYF 274
           +Q LP   + L L G Q +K K+D L  F
Sbjct: 75  FQNLPQLNEPLKLIGYQVDKTKDDELTSF 103
>sp|Q28987|CYTA8_PIG Cystatin A8 (Stefin A8)
          Length = 103

 Score = 65.1 bits (157), Expect = 5e-11
 Identities = 38/89 (42%), Positives = 54/89 (60%), Gaps = 2/89 (2%)
 Frame = +2

Query: 14  RTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRV 193
           R  + E + I   VKPQ+E++   +   F+ +  RSQVVAGTNY+ KVH+G   ++HI V
Sbjct: 15  RPATPEIQEIANKVKPQLEEKTKKTYEKFEAIIYRSQVVAGTNYYIKVHVGGNSYVHIIV 74

Query: 194 YQPLPGQGD-LALHGLQ-EKLKEDVLEYF 274
           +Q LP   + L L G Q +K K+D L  F
Sbjct: 75  FQNLPQLNEPLKLIGYQVDKTKDDELTGF 103
>sp|Q8I030|CYTB_PANTR Cystatin B (Stefin B)
          Length = 98

 Score = 64.7 bits (156), Expect = 6e-11
 Identities = 36/84 (42%), Positives = 49/84 (58%), Gaps = 2/84 (2%)
 Frame = +2

Query: 29  EEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRVYQPLP 208
           E + I   V+ Q+E++       F  +  +SQVVAGTNYF KVH+GD + +H+RV+Q LP
Sbjct: 15  ETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEEFVHLRVFQSLP 74

Query: 209 GQG-DLALHGLQ-EKLKEDVLEYF 274
            +   L L   Q  K K D L YF
Sbjct: 75  HENKPLTLSNYQTNKAKHDELTYF 98
>sp|P80416|CYTA_BOVIN Cystatin A (Stefin A) (Cystatin AS)
          Length = 98

 Score = 64.3 bits (155), Expect = 8e-11
 Identities = 35/84 (41%), Positives = 52/84 (61%), Gaps = 2/84 (2%)
 Frame = +2

Query: 29  EEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRVYQPLP 208
           E + I   VKPQ+E++   +   F  ++ +SQVVAG NY+ K+  GD ++IHI+V++ LP
Sbjct: 15  EIQEIANMVKPQLEEKTNETYEEFTAIEYKSQVVAGINYYIKIQTGDNRYIHIKVFKSLP 74

Query: 209 GQG-DLALHGLQ-EKLKEDVLEYF 274
            Q   L L G Q +K K+D L  F
Sbjct: 75  QQSHSLILTGYQVDKTKDDELAGF 98
>sp|P60576|CYTB_PONPY Cystatin B (Stefin B)
 sp|P60575|CYTB_PANPA Cystatin B (Stefin B)
 sp|Q76LA0|CYTB_GORGO Cystatin B (Stefin B)
 sp|P04080|CYTB_HUMAN Cystatin B (Liver thiol proteinase inhibitor) (CPI-B) (Stefin B)
          Length = 98

 Score = 63.9 bits (154), Expect = 1e-10
 Identities = 36/84 (42%), Positives = 48/84 (57%), Gaps = 2/84 (2%)
 Frame = +2

Query: 29  EEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRVYQPLP 208
           E + I   V+ Q+E++       F  +  +SQVVAGTNYF KVH+GD   +H+RV+Q LP
Sbjct: 15  ETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLP 74

Query: 209 GQG-DLALHGLQ-EKLKEDVLEYF 274
            +   L L   Q  K K D L YF
Sbjct: 75  HENKPLTLSNYQTNKAKHDELTYF 98
>sp|Q862Z5|CYTB_MACFU Cystatin B (Stefin B)
          Length = 98

 Score = 63.9 bits (154), Expect = 1e-10
 Identities = 36/90 (40%), Positives = 51/90 (56%), Gaps = 2/90 (2%)
 Frame = +2

Query: 11  VRTPSEEEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIR 190
           V+  + E + I   V+ Q+E++       F  +  +SQVVAGTNYF KVH+GD   +H+R
Sbjct: 9   VQPATAETQDIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLR 68

Query: 191 VYQPLPGQG-DLALHGLQ-EKLKEDVLEYF 274
           V++ LP +   L L   Q  K K D L YF
Sbjct: 69  VFKSLPHENKPLTLSDYQTNKAKHDELSYF 98
>sp|P01041|CYTB_RAT Cystatin B (Liver thiol proteinase inhibitor) (Stefin B) (Cystatin
           beta)
          Length = 98

 Score = 63.5 bits (153), Expect = 1e-10
 Identities = 35/84 (41%), Positives = 49/84 (58%), Gaps = 2/84 (2%)
 Frame = +2

Query: 29  EEKTILLTVKPQIEDQIGHSCSAFDILQLRSQVVAGTNYFAKVHIGDGKHIHIRVYQPLP 208
           E + I   VK Q+E++       F  +  R QVVAGTN+F KV +G+ K +H+RV++PLP
Sbjct: 15  ETQEIADKVKSQLEEKANQKFDVFKAISFRRQVVAGTNFFIKVDVGEEKCVHLRVFEPLP 74

Query: 209 GQG-DLALHGLQ-EKLKEDVLEYF 274
            +   L L   Q +K K D L YF
Sbjct: 75  HENKPLTLSSYQTDKEKHDELTYF 98
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,396,448
Number of Sequences: 369166
Number of extensions: 752687
Number of successful extensions: 1852
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1821
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1849
length of database: 68,354,980
effective HSP length: 89
effective length of database: 51,913,565
effective search space used: 1713147645
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)