Planaria EST Database

DrC_00843

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00843
         (1396 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9UI08|EVL_HUMAN  Ena/vasodilator-stimulated phosphoprote...    65   4e-10
sp|P70429|EVL_MOUSE  Ena/vasodilator-stimulated phosphoprote...    65   4e-10
sp|O08719|EVL_RAT  Ena/vasodilator-stimulated phosphoprotein...    62   5e-09
sp|P50552|VASP_HUMAN  Vasodilator-stimulated phosphoprotein ...    50   2e-05
sp|P70460|VASP_MOUSE  Vasodilator-stimulated phosphoprotein ...    46   2e-04
sp|P50551|VASP_CANFA  Vasodilator-stimulated phosphoprotein ...    42   0.003
sp|Q9NJG9|SUZ12_DROME  Polycomb protein Su(z)12 (Suppressor ...    39   0.036
sp|O00203|AP3B1_HUMAN  Adapter-related protein complex 3 bet...    36   0.23 
sp|O77459|KEN_DROME  Probable transcription factor Ken (Ken ...    36   0.23 
sp|P32583|SRP40_YEAST  Suppressor protein SRP40                    35   0.52 

>sp|Q9UI08|EVL_HUMAN Ena/vasodilator-stimulated phosphoprotein-like protein (Ena/VASP-like
            protein)
          Length = 416

 Score = 65.5 bits (158), Expect = 4e-10
 Identities = 60/196 (30%), Positives = 94/196 (47%), Gaps = 16/196 (8%)
 Frame = +2

Query: 533  SDEESNQSGLASQLRQAKLKSRGGPVEPDVSSNQNKNVTSSATLGRA----GGDSMMSEM 700
            S +ES+ SGLA+ +  AKL+    P   D S   + + TS +   RA    GG  +M EM
Sbjct: 214  SHDESSMSGLAAAIAGAKLRRVQRP--EDASGGSSPSGTSKSDANRASSGGGGGGLMEEM 271

Query: 701  QRVLAARKKA-KEVVKPTGSECTDNGGSGDNSSKNPQWR---QNHHRSQSDCDQTNGTNN 868
             ++LA R+KA  +  KP   +  ++     ++S +P  R   Q  + S++       +N+
Sbjct: 272  NKLLAKRRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNSSEAGRKPWERSNS 331

Query: 869  QSKPAADAAKSTSMQCATLGRKGSLQ--PDSQ-QPVSSITRQ-----DLDNFKREILQEV 1024
              KP +     T     +   K  LQ  P S+ +P  S+        DLD  K+EIL+EV
Sbjct: 332  VEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEV 391

Query: 1025 RSIIQQFKADIIDVIR 1072
               + + K +IID IR
Sbjct: 392  VRELHKVKEEIIDAIR 407

>sp|P70429|EVL_MOUSE Ena/vasodilator-stimulated phosphoprotein-like protein (Ena/VASP-like
            protein)
          Length = 414

 Score = 65.5 bits (158), Expect = 4e-10
 Identities = 60/194 (30%), Positives = 93/194 (47%), Gaps = 16/194 (8%)
 Frame = +2

Query: 539  EESNQSGLASQLRQAKLKSRGGPVEPDVSSNQNKNVTSSATLGRA----GGDSMMSEMQR 706
            +ES+ SGLA+ L  AKL+    P   D S   + + TS +   RA    GG  +M EM +
Sbjct: 214  DESSASGLAAALAGAKLRRVQRP--EDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNK 271

Query: 707  VLAARKKA-KEVVKPTGSECTDNGGSGDNSSKNPQWR---QNHHRSQSDCDQTNGTNNQS 874
            +LA R+KA  +  KP   +  ++     ++S +P  R   Q  + S++       +N+  
Sbjct: 272  LLAKRRKAASQTDKPADRKEDESQTEDPSTSPSPGTRATSQPPNSSEAGRKPWERSNSVE 331

Query: 875  KPAADAAKSTSMQCATLGRKGSLQ--PDSQ-QPVSSIT-----RQDLDNFKREILQEVRS 1030
            KP +     T     +   K  LQ  P S+ +P  S+        DLD  K+EIL+EV  
Sbjct: 332  KPVSSLLSRTPSVAKSPEAKSPLQSQPHSRVKPAGSVNDVGLDALDLDRMKQEILEEVVR 391

Query: 1031 IIQQFKADIIDVIR 1072
             + + K +IID IR
Sbjct: 392  ELHKVKEEIIDAIR 405

>sp|O08719|EVL_RAT Ena/vasodilator-stimulated phosphoprotein-like protein (Ena/VASP-like
            protein)
          Length = 393

 Score = 61.6 bits (148), Expect = 5e-09
 Identities = 54/183 (29%), Positives = 85/183 (46%), Gaps = 5/183 (2%)
 Frame = +2

Query: 539  EESNQSGLASQLRQAKLKSRGGPVEPDVSSNQNKNVTSSATLGRA----GGDSMMSEMQR 706
            +ES+ SGLA+ L  AKL+    P   D S   + + TS +   RA    GG  +M EM +
Sbjct: 214  DESSASGLAAALAGAKLRRVQRP--EDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNK 271

Query: 707  VLAARKKA-KEVVKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCDQTNGTNNQSKPA 883
            +LA R+KA  +  KP   +  +N     ++S +P        S++     N +    KP 
Sbjct: 272  LLAKRRKAASQTDKPADRKEDENQTEDPSTSPSPG-------SRATSQPPNSSEAGRKPW 324

Query: 884  ADAAKSTSMQCATLGRKGSLQPDSQQPVSSITRQDLDNFKREILQEVRSIIQQFKADIID 1063
                +S S++         ++P        +   DLD  K+EIL+EV   + + K +IID
Sbjct: 325  E---RSNSVEKPVSSLLSRVKPAGSVNDVGLDALDLDRMKQEILEEVVRELHKVKEEIID 381

Query: 1064 VIR 1072
             IR
Sbjct: 382  AIR 384

>sp|P50552|VASP_HUMAN Vasodilator-stimulated phosphoprotein (VASP)
          Length = 380

 Score = 50.1 bits (118), Expect = 2e-05
 Identities = 44/176 (25%), Positives = 77/176 (43%), Gaps = 7/176 (3%)
 Frame = +2

Query: 557  GLASQLRQAKLK-------SRGGPVEPDVSSNQNKNVTSSATLGRAGGDSMMSEMQRVLA 715
            GLA+ +  AKL+       + GGP  P   S            GR+GG  +M EM  +LA
Sbjct: 225  GLAAAIAGAKLRKVSKQEEASGGPTAPKAES------------GRSGGGGLMEEMNAMLA 272

Query: 716  ARKKAKEVVKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCDQTNGTNNQSKPAADAA 895
             R+KA +V + T  +  ++    +  ++ P   ++  R     ++ + T  + K ++   
Sbjct: 273  RRRKATQVGEKTPKD--ESANQEEPEARVPAQSESVRR---PWEKNSTTLPRMKSSSSVT 327

Query: 896  KSTSMQCATLGRKGSLQPDSQQPVSSITRQDLDNFKREILQEVRSIIQQFKADIID 1063
             S +  C                 SS    DL   K+E+L+EV+  +Q+ K +II+
Sbjct: 328  TSETQPCTP---------------SSSDYSDLQRVKQELLEEVKKELQKVKEEIIE 368

>sp|P70460|VASP_MOUSE Vasodilator-stimulated phosphoprotein (VASP)
          Length = 376

 Score = 46.2 bits (108), Expect = 2e-04
 Identities = 45/183 (24%), Positives = 74/183 (40%), Gaps = 9/183 (4%)
 Frame = +2

Query: 545  SNQSGLASQLRQAKLK-------SRGGPVEPDVSSNQNKNVTSSATLGRAGGDSMMSEMQ 703
            S   GLA+ +  AKL+       + GGP+ P   ++            R+ G  +M EM 
Sbjct: 217  SGAPGLAAAIAGAKLRKVSKQEEASGGPLAPKAENS------------RSTGGGLMEEMN 264

Query: 704  RVLAARKKAKEVVKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCDQTNGTNNQSKPA 883
             +LA R+KA +V              G+   K+    Q    ++           QS+P 
Sbjct: 265  AMLARRRKATQV--------------GEKPPKDESASQEESEARLPA--------QSEPV 302

Query: 884  ADAAKSTSMQCATLGRKGSLQPDSQQPVS--SITRQDLDNFKREILQEVRSIIQQFKADI 1057
                +  S     +    S+      P +  S    DL+  K+E+L+EVR  +Q+ K +I
Sbjct: 303  RRPWEKNSTTLPRMKSSSSVTTSEAHPSTPCSSDDSDLERVKQELLEEVRKELQKMKEEI 362

Query: 1058 IDV 1066
            I+V
Sbjct: 363  IEV 365

>sp|P50551|VASP_CANFA Vasodilator-stimulated phosphoprotein (VASP)
          Length = 384

 Score = 42.4 bits (98), Expect = 0.003
 Identities = 46/177 (25%), Positives = 75/177 (42%), Gaps = 8/177 (4%)
 Frame = +2

Query: 557  GLASQLRQAKLK-------SRGGPVEPDVSSNQNKNVTSSATLGRAGGDSMMSEMQRVLA 715
            GLA+ +  AKL+       + GGP  P   S             R+ G  +M EM  +LA
Sbjct: 228  GLAAAIAGAKLRKVSKQEEASGGPPVPKAEST------------RSTGGGLMEEMNAMLA 275

Query: 716  ARKKAKEV-VKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCDQTNGTNNQSKPAADA 892
             R+KA +V  KP   E         ++++ P+ R           +    N+ + P   +
Sbjct: 276  RRRKATQVGEKPPKDE---------SANEEPEARVPVPAQSETVRRPWEKNSTTLPRMKS 326

Query: 893  AKSTSMQCATLGRKGSLQPDSQQPVSSITRQDLDNFKREILQEVRSIIQQFKADIID 1063
            + S +   A         P +    SS    DL+  K+E+L+EVR  +Q+ K +II+
Sbjct: 327  SSSVTTSEA--------HPSTP---SSSDESDLERVKQELLEEVRKELQKVKEEIIE 372

>sp|Q9NJG9|SUZ12_DROME Polycomb protein Su(z)12 (Suppressor 12 of zeste protein)
          Length = 900

 Score = 38.9 bits (89), Expect = 0.036
 Identities = 32/139 (23%), Positives = 56/139 (40%), Gaps = 1/139 (0%)
 Frame = +2

Query: 524  NNDSDEESNQSGLASQLRQAKLKSRGGPVEPDVSSNQNKNVTSSATLGR-AGGDSMMSEM 700
            N  +   SN   +A +     L +     E      Q +NV+ S      A    + S+ 
Sbjct: 746  NGSNSSSSNSKNVAKKSADQPLSTLANTRERRSEYGQKRNVSGSRLAATPASKRKLSSKD 805

Query: 701  QRVLAARKKAKEVVKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCDQTNGTNNQSKP 880
              VL  R++  +    TG      GGSG  +++N   + N+H   +  +  + +++ SK 
Sbjct: 806  NTVLNKRQRYSDGSPGTGIGNGHGGGSGSGANRN---KSNNHSLPATSNNASSSSSNSKR 862

Query: 881  AADAAKSTSMQCATLGRKG 937
            A    +STS +    G  G
Sbjct: 863  AIARRRSTSERTKASGSTG 881


 Score = 33.5 bits (75), Expect = 1.5
 Identities = 23/77 (29%), Positives = 39/77 (50%), Gaps = 1/77 (1%)
 Frame = +2

Query: 575 RQAKLKSRGGPVEPDVSSNQNKNVTSSATLGRAGGDSMMSEMQRVLAARKKAKEVVKP-T 751
           +Q   K+   P +P   ++Q  + +S++T G   G S M   +R+ A  K+      P  
Sbjct: 678 KQEDPKTLKSPQKPAPPADQ-ASTSSASTSGSGSGSSSMQPPKRMPAHLKRGSAASSPGV 736

Query: 752 GSECTDNGGSGDNSSKN 802
            S+ T+NG +G NSS +
Sbjct: 737 QSKGTENGTNGSNSSSS 753

>sp|O00203|AP3B1_HUMAN Adapter-related protein complex 3 beta 1 subunit (Beta3A-adaptin)
            (Adaptor protein complex AP-3 beta-1 subunit) (AP-3
            complex beta-1 subunit) (Clathrin assembly protein
            complex 3 beta-1 large chain)
          Length = 1094

 Score = 36.2 bits (82), Expect = 0.23
 Identities = 33/165 (20%), Positives = 67/165 (40%)
 Frame = +2

Query: 506  AKPFQQNNDSDEESNQSGLASQLRQAKLKSRGGPVEPDVSSNQNKNVTSSATLGRAGGDS 685
            AK F   ++ +E+S+ S   S+          G    +  SN++ +  SS+      G  
Sbjct: 672  AKKFYSESEEEEDSSDSSSDSESESGSESGEQGESGEEGDSNEDSSEDSSSEQDSESGRE 731

Query: 686  MMSEMQRVLAARKKAKEVVKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCDQTNGTN 865
               E +R      KAK      G   +++G   +  SK      ++  S S  D ++ + 
Sbjct: 732  SGLENKRTAKRNSKAK------GKSDSEDGEKENEKSKTSD--SSNDESSSIEDSSSDSE 783

Query: 866  NQSKPAADAAKSTSMQCATLGRKGSLQPDSQQPVSSITRQDLDNF 1000
            ++S+P ++    +  +  T  ++   + D       ++  DLD+F
Sbjct: 784  SESEPESE----SESRRVTKEKEKKTKQDRTPLTKDVSLLDLDDF 824

>sp|O77459|KEN_DROME Probable transcription factor Ken (Ken and Barbie protein)
          Length = 601

 Score = 36.2 bits (82), Expect = 0.23
 Identities = 24/93 (25%), Positives = 41/93 (44%), Gaps = 7/93 (7%)
 Frame = +2

Query: 629 NQNKNVTSSATLGRAGGDSMMSEMQRVLAAR-----KKAKEVVKPTGSECTDNGGSGDN- 790
           N N N  S+A   R+GG +   E +     +     K   E+V  + +  +   GS  N 
Sbjct: 180 NSNDNCESAAECERSGGHNNKEEDEDDCTHKDNKSDKDTDEIVNLSNAPPSGTSGSNSNI 239

Query: 791 -SSKNPQWRQNHHRSQSDCDQTNGTNNQSKPAA 886
            +S N Q +Q+HH    + +  N  NN +  ++
Sbjct: 240 STSSNHQQQQHHHHHHHNHNNNNNNNNNNSSSS 272

>sp|P32583|SRP40_YEAST Suppressor protein SRP40
          Length = 406

 Score = 35.0 bits (79), Expect = 0.52
 Identities = 26/140 (18%), Positives = 65/140 (46%), Gaps = 1/140 (0%)
 Frame = +2

Query: 491 STITMAKPFQQNNDSDEESNQSGLASQLRQ-AKLKSRGGPVEPDVSSNQNKNVTSSATLG 667
           S+ + +   + +++SD +S+ S  +S     +   S     + D SS+ + + + S +  
Sbjct: 179 SSSSSSSDSESDSESDSQSSSSSSSSDSSSDSDSSSSDSSSDSDSSSSSSSSSSDSDSDS 238

Query: 668 RAGGDSMMSEMQRVLAARKKAKEVVKPTGSECTDNGGSGDNSSKNPQWRQNHHRSQSDCD 847
            +  DS  S      ++   + +  + T S+ +D+    D+ S +    +     +S  +
Sbjct: 239 DSSSDSDSSGSSDSSSSSDSSSD--ESTSSDSSDSDSDSDSGSSSELETKEATADESKAE 296

Query: 848 QTNGTNNQSKPAADAAKSTS 907
           +T  ++N+S P+A ++ S +
Sbjct: 297 ETPASSNESTPSASSSSSAN 316

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 129,679,515
Number of Sequences: 369166
Number of extensions: 2395025
Number of successful extensions: 6810
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6324
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6769
length of database: 68,354,980
effective HSP length: 114
effective length of database: 47,295,190
effective search space used: 16553316500
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00843

  1. Dr_sW_021_I03
  2. Dr_sW_028_C24
  3. Dr_sW_009_C07