Planaria EST Database

DrC_00717

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00717
         (430 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9XSK2|CD63_BOVIN  CD63 antigen                                 43   2e-04
sp|O70401|TSN6_MOUSE  Tetraspanin-6 (Tspan-6) (Transmembrane...    42   5e-04
sp|O43657|TSN6_HUMAN  Tetraspanin-6 (Tspan-6) (Transmembrane...    40   0.003
sp|P08962|CD63_HUMAN  CD63 antigen (Melanoma-associated anti...    38   0.008
sp|Q9QY33|TSN3_MOUSE  Tetraspanin-3 (Tspan-3) (Transmembrane...    38   0.010
sp|O60637|TSN3_HUMAN  Tetraspanin-3 (Tspan-3) (Transmembrane...    37   0.013
sp|P41731|CD63_MOUSE  CD63 antigen                                 37   0.023
sp|P28648|CD63_RAT  CD63 antigen (AD1 antigen)                     36   0.039
sp|Q61451|CD53_MOUSE  Leukocyte surface antigen CD53 (Cell s...    36   0.039
sp|Q62283|TSN7_MOUSE  Tetraspanin-7 (Tspan-7) (Transmembrane...    35   0.050

>sp|Q9XSK2|CD63_BOVIN CD63 antigen
          Length = 237

 Score = 43.1 bits (100), Expect = 2e-04
 Identities = 20/77 (25%), Positives = 40/77 (51%), Gaps = 9/77 (11%)
 Frame = +3

Query: 15  IDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSC-----YSLGGSI----LHTAGCRSK 167
           +D++Q  F CCG+ +++DW   +  +N    P SC     ++ G +     +HT GC  K
Sbjct: 136 LDKMQKDFECCGAANYTDWEKILAVTNKV--PDSCCVNITHNCGINFVVKDIHTEGCVEK 193

Query: 168 LYDWYRRFFIAILSVPI 218
           +  W R+  + +++  +
Sbjct: 194 IAAWLRKNVLVVVAAAL 210

>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
          Length = 245

 Score = 42.0 bits (97), Expect = 5e-04
 Identities = 17/43 (39%), Positives = 25/43 (58%)
 Frame = +3

Query: 6   SRDIDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSLGG 134
           S  +D++Q+   CCG  ++ DW  T +YS  T +P SC  L G
Sbjct: 141 SEAVDKIQSTLHCCGVTNYGDWKGTNYYS-ETGFPKSCCKLEG 182

>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
           (T245 protein) (Tetraspanin TM4-D) (A15 homolog)
          Length = 245

 Score = 39.7 bits (91), Expect = 0.003
 Identities = 16/41 (39%), Positives = 22/41 (53%)
 Frame = +3

Query: 6   SRDIDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSL 128
           S  +D++QN   CCG   + DW  T +YS    +P SC  L
Sbjct: 141 SHAVDKIQNTLHCCGVTDYRDWTDTNYYSEK-GFPKSCCKL 180

>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
           (Lysosome-associated membrane glycoprotein 3) (LAMP-3)
           (Ocular melanoma-associated antigen) (OMA81H)
           (Granulophysin) (Tetraspanin-30) (Tspan-30)
          Length = 238

 Score = 38.1 bits (87), Expect = 0.008
 Identities = 18/77 (23%), Positives = 33/77 (42%), Gaps = 9/77 (11%)
 Frame = +3

Query: 15  IDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSC---------YSLGGSILHTAGCRSK 167
           +D +Q  F CCG+ +++DW      S +   P SC          +     +H  GC  K
Sbjct: 136 LDRMQADFKCCGAANYTDWEKIPSMSKN-RVPDSCCINVTVGCGINFNEKAIHKEGCVEK 194

Query: 168 LYDWYRRFFIAILSVPI 218
           +  W R+  + + +  +
Sbjct: 195 IGGWLRKNVLVVAAAAL 211

>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
           (Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
          Length = 253

 Score = 37.7 bits (86), Expect = 0.010
 Identities = 22/65 (33%), Positives = 33/65 (50%), Gaps = 12/65 (18%)
 Frame = +3

Query: 6   SRDIDELQNHFACCGSNSFSDWNSTVFYSNSTN--YPSSC-----YSLGGSI-----LHT 149
           SR ID +Q    CCG +++SDW +T ++  + N   P SC      S  GS+     L+ 
Sbjct: 135 SRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETAKSCNGSLANPSDLYA 194

Query: 150 AGCRS 164
            GC +
Sbjct: 195 EGCEA 199

>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
           (Tetraspanin TM4-A)
          Length = 253

 Score = 37.4 bits (85), Expect = 0.013
 Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 7/54 (12%)
 Frame = +3

Query: 6   SRDIDELQNHFACCGSNSFSDWNSTVFYSNSTN--YPSSC-----YSLGGSILH 146
           SR ID +Q    CCG +++SDW +T ++  + N   P SC      +  GS+ H
Sbjct: 135 SRAIDYVQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETASNCNGSLAH 188

>sp|P41731|CD63_MOUSE CD63 antigen
          Length = 238

 Score = 36.6 bits (83), Expect = 0.023
 Identities = 17/76 (22%), Positives = 35/76 (46%), Gaps = 8/76 (10%)
 Frame = +3

Query: 15  IDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSL--------GGSILHTAGCRSKL 170
           +D+LQ    CCG+++++DW +    +      S C ++          S +HT GC   +
Sbjct: 136 LDKLQKENNCCGASNYTDWENIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETI 195

Query: 171 YDWYRRFFIAILSVPI 218
             W R+  + + +  +
Sbjct: 196 AIWLRKNILLVAAAAL 211

>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
          Length = 238

 Score = 35.8 bits (81), Expect = 0.039
 Identities = 17/66 (25%), Positives = 30/66 (45%), Gaps = 8/66 (12%)
 Frame = +3

Query: 15  IDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSL--------GGSILHTAGCRSKL 170
           +D+LQ    CCG+++++DW      +      S C ++          S +HT GC   +
Sbjct: 136 LDKLQKENKCCGASNYTDWERIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETI 195

Query: 171 YDWYRR 188
             W R+
Sbjct: 196 AAWLRK 201

>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
          Length = 219

 Score = 35.8 bits (81), Expect = 0.039
 Identities = 22/62 (35%), Positives = 27/62 (43%)
 Frame = +3

Query: 18  DELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSLGGSILHTAGCRSKLYDWYRRFFI 197
           D +Q    CCG N  SDW S          PSSC S G  +    GC +K   W+   F+
Sbjct: 136 DFIQTQLQCCGVNGSSDWTS--------GPPSSCPS-GADV---QGCYNKAKSWFHSNFL 183

Query: 198 AI 203
            I
Sbjct: 184 YI 185

>sp|Q62283|TSN7_MOUSE Tetraspanin-7 (Tspan-7) (Transmembrane 4 superfamily member 2)
           (Cell surface glycoprotein A15) (PE31) (TALLA homolog)
          Length = 249

 Score = 35.4 bits (80), Expect = 0.050
 Identities = 12/38 (31%), Positives = 24/38 (63%)
 Frame = +3

Query: 6   SRDIDELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSC 119
           SR +D +Q   +CCG  ++++W+S+ ++ +    PS C
Sbjct: 138 SRAVDHVQRSLSCCGVQNYTNWSSSPYFLDHGIPPSCC 175

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,101,119
Number of Sequences: 369166
Number of extensions: 921499
Number of successful extensions: 2429
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2425
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2095022160
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00717

  1. Dr_sW_018_L20
  2. Dr_sW_024_E11
  3. Dr_sW_004_A22
  4. Dr_sW_004_B21