Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_A22
(404 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9XSK2|CD63_BOVIN CD63 antigen 40 0.002
sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane... 38 0.009
sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane... 35 0.044
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 35 0.057
sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell s... 35 0.074
sp|P41731|CD63_MOUSE CD63 antigen 33 0.17
sp|P22533|MANB_CALSA Beta-mannanase/endoglucanase A precurs... 33 0.17
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 33 0.28
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell sur... 32 0.37
sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane... 32 0.48
>sp|Q9XSK2|CD63_BOVIN CD63 antigen
Length = 237
Score = 40.0 bits (92), Expect = 0.002
Identities = 19/75 (25%), Positives = 38/75 (50%), Gaps = 9/75 (12%)
Frame = +2
Query: 2 ELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSC-----YSLGGSI----LHTAGCRSKLY 154
++Q F CCG+ +++DW + +N P SC ++ G + +HT GC K+
Sbjct: 138 KMQKDFECCGAANYTDWEKILAVTNKV--PDSCCVNITHNCGINFVVKDIHTEGCVEKIA 195
Query: 155 DWYRRFFIAILSVPI 199
W R+ + +++ +
Sbjct: 196 AWLRKNVLVVVAAAL 210
>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
Length = 245
Score = 37.7 bits (86), Expect = 0.009
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +2
Query: 2 ELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSLGG 115
++Q+ CCG ++ DW T +YS T +P SC L G
Sbjct: 146 KIQSTLHCCGVTNYGDWKGTNYYS-ETGFPKSCCKLEG 182
>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
(T245 protein) (Tetraspanin TM4-D) (A15 homolog)
Length = 245
Score = 35.4 bits (80), Expect = 0.044
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 2 ELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSL 109
++QN CCG + DW T +YS +P SC L
Sbjct: 146 KIQNTLHCCGVTDYRDWTDTNYYSEK-GFPKSCCKL 180
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 35.0 bits (79), Expect = 0.057
Identities = 17/74 (22%), Positives = 31/74 (41%), Gaps = 9/74 (12%)
Frame = +2
Query: 5 LQNHFACCGSNSFSDWNSTVFYSNSTNYPSSC---------YSLGGSILHTAGCRSKLYD 157
+Q F CCG+ +++DW S + P SC + +H GC K+
Sbjct: 139 MQADFKCCGAANYTDWEKIPSMSKN-RVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGG 197
Query: 158 WYRRFFIAILSVPI 199
W R+ + + + +
Sbjct: 198 WLRKNVLVVAAAAL 211
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
Length = 219
Score = 34.7 bits (78), Expect = 0.074
Identities = 21/60 (35%), Positives = 26/60 (43%)
Frame = +2
Query: 5 LQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSLGGSILHTAGCRSKLYDWYRRFFIAI 184
+Q CCG N SDW S PSSC S G + GC +K W+ F+ I
Sbjct: 138 IQTQLQCCGVNGSSDWTS--------GPPSSCPS-GADV---QGCYNKAKSWFHSNFLYI 185
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 33.5 bits (75), Expect = 0.17
Identities = 16/74 (21%), Positives = 33/74 (44%), Gaps = 8/74 (10%)
Frame = +2
Query: 2 ELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSL--------GGSILHTAGCRSKLYD 157
+LQ CCG+++++DW + + S C ++ S +HT GC +
Sbjct: 138 KLQKENNCCGASNYTDWENIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAI 197
Query: 158 WYRRFFIAILSVPI 199
W R+ + + + +
Sbjct: 198 WLRKNILLVAAAAL 211
>sp|P22533|MANB_CALSA Beta-mannanase/endoglucanase A precursor [Includes: Mannan
endo-1,4-beta-mannosidase A (Beta-mannanase)
(Endo-1,4-mannanase); Endo-1,4-beta-glucanase
(Cellulase)]
Length = 1331
Score = 33.5 bits (75), Expect = 0.17
Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 2/78 (2%)
Frame = -1
Query: 272 DDNFELSIYVIGGVRFPRTKRL*WVSEQRGS--Q*RTAGTNHTVLIDNLLCAEWSPPTSN 99
D N + I IG + WV++ + + R AG HT+++D W SN
Sbjct: 149 DGNEDFVIINIGNEPYGNNNYQNWVNDTKNAIKALRDAGFKHTIMVD---APNWGQDWSN 205
Query: 98 TRRDNLYCLSRTQSSSNL 45
T RDN + NL
Sbjct: 206 TMRDNAQSIMEADPLRNL 223
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 32.7 bits (73), Expect = 0.28
Identities = 16/64 (25%), Positives = 28/64 (43%), Gaps = 8/64 (12%)
Frame = +2
Query: 2 ELQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSL--------GGSILHTAGCRSKLYD 157
+LQ CCG+++++DW + S C ++ S +HT GC +
Sbjct: 138 KLQKENKCCGASNYTDWERIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAA 197
Query: 158 WYRR 169
W R+
Sbjct: 198 WLRK 201
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Leukocyte antigen MRC OX-44)
Length = 219
Score = 32.3 bits (72), Expect = 0.37
Identities = 23/65 (35%), Positives = 28/65 (43%)
Frame = +2
Query: 5 LQNHFACCGSNSFSDWNSTVFYSNSTNYPSSCYSLGGSILHTAGCRSKLYDWYRRFFIAI 184
+Q+ CCG N SDW S PSSC S G + GC K W+ F+ I
Sbjct: 138 IQSQLQCCGVNGSSDWIS--------GPPSSCPS-GADV---QGCYKKGQAWFHSNFLYI 185
Query: 185 LSVPI 199
V I
Sbjct: 186 GIVTI 190
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
Length = 253
Score = 32.0 bits (71), Expect = 0.48
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 12/59 (20%)
Frame = +2
Query: 5 LQNHFACCGSNSFSDWNSTVFYSNSTN--YPSSC-----YSLGGSI-----LHTAGCRS 145
+Q CCG +++SDW +T ++ + N P SC S GS+ L+ GC +
Sbjct: 141 VQRQLHCCGIHNYSDWENTDWFKETKNQSVPLSCCRETAKSCNGSLANPSDLYAEGCEA 199
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,340,620
Number of Sequences: 369166
Number of extensions: 864549
Number of successful extensions: 2239
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2235
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1809034200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)