Planaria EST Database

DrC_00707

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00707
         (914 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P23492|PNPH_MOUSE  Purine nucleoside phosphorylase (Inosi...   290   3e-78
sp|P00491|PNPH_HUMAN  Purine nucleoside phosphorylase (Inosi...   288   1e-77
sp|P55859|PNPH_BOVIN  Purine nucleoside phosphorylase (Inosi...   283   6e-76
sp|P77834|PUNA_BACST  Purine nucleoside phosphorylase I (PNP...   221   2e-57
sp|Q05788|PNPH_YEAST  Purine nucleoside phosphorylase (Inosi...   218   2e-56
sp|P46354|PUNA_BACSU  Purine nucleoside phosphorylase I (PNP...   199   7e-51
sp|P45563|XAPA_ECOLI  Xanthosine phosphorylase                    174   4e-43
sp|P46862|PUNA_MYCLE  Purine nucleoside phosphorylase (Inosi...   158   2e-38
sp|P0A539|PUNA_MYCBO  Purine nucleoside phosphorylase (Inosi...   154   4e-37
sp|P81989|PUNA_CELSP  Purine nucleoside phosphorylase (Inosi...   122   1e-27

>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 289

 Score =  290 bits (743), Expect = 3e-78
 Identities = 140/271 (51%), Positives = 192/271 (70%)
 Frame = +2

Query: 8   ANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHL 187
           A +LL  T++RP++AVIC              QI  Y+EI  FP+STV GHAG LVFG L
Sbjct: 14  AKWLLQHTEYRPQVAVICGSGLGGLTAHLKEAQIFDYNEIPNFPQSTVQGHAGRLVFGLL 73

Query: 188 GGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIK 367
            G+  V+MQGRFH YEGY+      PVRV +L+GV+ L++TNAAGGLNP ++VGD+M+I+
Sbjct: 74  NGRCCVMMQGRFHMYEGYSLSKVTFPVRVFHLLGVETLVVTNAAGGLNPNFEVGDIMLIR 133

Query: 368 DHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYA 547
           DHIN PG CG NPL GPN+++FG RF  +SDAYD+++R KA     ++GE +++ EG Y 
Sbjct: 134 DHINLPGFCGQNPLRGPNDERFGVRFPAMSDAYDRDMRQKAFTAWKQMGEQRKLQEGTYV 193

Query: 548 MQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEK 727
           M  GP +ETV+E +++KMLG DA GMST  EVIVARH G+ V G SLITN+ V+++++ +
Sbjct: 194 MLAGPNFETVAESRLLKMLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVVMDYENLE 253

Query: 728 KANHLEVLAVAKERAQIMQTLISKLVEILQI 820
           KANH+EVL   K  AQ ++  +S L+E + +
Sbjct: 254 KANHMEVLDAGKAAAQTLERFVSILMESIPL 284

>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 289

 Score =  288 bits (737), Expect = 1e-77
 Identities = 138/266 (51%), Positives = 186/266 (69%)
 Frame = +2

Query: 8   ANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHL 187
           A +LL  TK RP++A+IC              QI  YSEI  FP+STV GHAG LVFG L
Sbjct: 14  AEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFL 73

Query: 188 GGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIK 367
            G+  V+MQGRFH YEGY       PVRV +L+GV  L++TNAAGGLNP ++VGD+M+I+
Sbjct: 74  NGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIR 133

Query: 368 DHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYA 547
           DHIN PG  G NPL GPN+++FG+RF  +SDAYD+ +R +A     ++GE + + EG Y 
Sbjct: 134 DHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYV 193

Query: 548 MQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEK 727
           M  GP++ETV+EC++++ LG DA GMST  EVIVARH G+ V G SLITN+ +++++S +
Sbjct: 194 MVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLE 253

Query: 728 KANHLEVLAVAKERAQIMQTLISKLV 805
           KANH EVLA  K+ AQ ++  +S L+
Sbjct: 254 KANHEEVLAAGKQAAQKLEQFVSILM 279

>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 289

 Score =  283 bits (723), Expect = 6e-76
 Identities = 138/273 (50%), Positives = 186/273 (68%)
 Frame = +2

Query: 2   EIANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFG 181
           + A +LL  T+ RP++AVIC              Q   YSEI  FP+STV GHAG LVFG
Sbjct: 12  DTAKWLLSHTEQRPQVAVICGSGLGGLVNKLTQAQTFDYSEIPNFPESTVPGHAGRLVFG 71

Query: 182 HLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMI 361
            L G+  V+MQGRFH YEGY       PVRV  L+GV+ L++TNAAGGLNP ++VGD+M+
Sbjct: 72  ILNGRACVMMQGRFHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGLNPNFEVGDIML 131

Query: 362 IKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGV 541
           I+DHIN PG  G NPL GPNE++FG RF  +SDAYD+++R KA     ++GE + + EG 
Sbjct: 132 IRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHSTWKQMGEQRELQEGT 191

Query: 542 YAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDS 721
           Y M  GP +ETV+EC++++ LG DA GMST  EVIVARH G+ V G SLITN+ +++++S
Sbjct: 192 YVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYES 251

Query: 722 EKKANHLEVLAVAKERAQIMQTLISKLVEILQI 820
           + KANH EVL   K+ AQ ++  +S L+  + +
Sbjct: 252 QGKANHEEVLEAGKQAAQKLEQFVSLLMASIPV 284

>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
           phosphorylase)
          Length = 274

 Score =  221 bits (563), Expect = 2e-57
 Identities = 119/232 (51%), Positives = 149/232 (64%)
 Frame = +2

Query: 2   EIANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFG 181
           + A FL  K    P+I +I                 IPYS+I  FP STV GHAG LV+G
Sbjct: 8   QAAQFLKEKFPTSPQIGLILGSGLGVLADEIEQAIKIPYSDIPNFPVSTVEGHAGQLVYG 67

Query: 182 HLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMI 361
            L G  +V+MQGRFH YEGY+      PVRV+  +GV+ L++TNAAGG+N  ++ GD+MI
Sbjct: 68  QLEGATVVVMQGRFHYYEGYSFDKVTFPVRVMKALGVEQLIVTNAAGGVNESFEPGDLMI 127

Query: 362 IKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGV 541
           I DHIN     G NPL GPN+   G RF  +S+AY K LR  AK++AN +G   RV EGV
Sbjct: 128 ISDHIN---NMGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIG--LRVREGV 182

Query: 542 YAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 697
           Y    GPAYET +E +M++++GGDA GMST  EVIVARH GM VLGIS I+N
Sbjct: 183 YVANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISN 234

>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 311

 Score =  218 bits (555), Expect = 2e-56
 Identities = 118/244 (48%), Positives = 154/244 (63%), Gaps = 12/244 (4%)
 Frame = +2

Query: 110 IPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIG 289
           +PY +I GF KSTV GH+G L+FG + G  +VLM GR H YEG T      P+RVLN +G
Sbjct: 65  VPYQDIPGFKKSTVPGHSGTLMFGSMNGSPVVLMNGRLHGYEGNTLFETTFPIRVLNHMG 124

Query: 290 -VKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAY 466
            V+ L++TNAAGG+N  Y+  D+M I DH+N PGL G +PL GPN D+ G RF+ LSDAY
Sbjct: 125 HVRNLIVTNAAGGINAKYQACDLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAY 184

Query: 467 DKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVI 646
           D  LR    +   +L   + +HEG Y    GP +ET +E KM++MLGGDA GMST  EVI
Sbjct: 185 DLELRKLLFKKWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVPEVI 244

Query: 647 VARHMGMSVLGISLITNECVLEHDS-----------EKKANHLEVLAVAKERAQIMQTLI 793
           VARH G  VL +SLITN CV++  +           + KA H EVL   K  +  +Q LI
Sbjct: 245 VARHCGWRVLALSLITNTCVVDSPASALDESPVPLEKGKATHAEVLENGKIASNDVQNLI 304

Query: 794 SKLV 805
           + ++
Sbjct: 305 AAVM 308

>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
           phosphorylase)
          Length = 271

 Score =  199 bits (507), Expect = 7e-51
 Identities = 112/242 (46%), Positives = 147/242 (60%)
 Frame = +2

Query: 41  PKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGR 220
           PKI +I                 + Y +I  FP STV GHAG LV G L G  ++ MQGR
Sbjct: 20  PKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTLEGVSVIAMQGR 79

Query: 221 FHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGF 400
           FH YEGY+ +    PVRV+  +GV+ L++TNAAGG+N  ++ GD+MII DHINF    G 
Sbjct: 80  FHFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIITDHINF---MGT 136

Query: 401 NPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVS 580
           NPL GPNE  FG RF  +S AYDK+L   A++IA  L  +  + +GVY    GP+YET +
Sbjct: 137 NPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDL--NIPIQKGVYTAVTGPSYETPA 194

Query: 581 ECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVA 760
           E + ++ +G DA GMST  EVIVA H GM VLGIS I+N      D  +  +H EV+ V 
Sbjct: 195 EVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD--QPLSHDEVMEVT 252

Query: 761 KE 766
           ++
Sbjct: 253 EK 254

>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
          Length = 277

 Score =  174 bits (440), Expect = 4e-43
 Identities = 103/257 (40%), Positives = 140/257 (54%), Gaps = 1/257 (0%)
 Frame = +2

Query: 26  KTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIV 205
           K  F P++A I                 I Y ++ GFP STV GHAG LV GHL G  +V
Sbjct: 21  KPDFTPRVAFILGSGLGALADQIENAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVV 80

Query: 206 LMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHIN-F 382
            M+GR H YEG         +R   L+G + L  TNAAG L P    G ++ +KDHIN  
Sbjct: 81  CMKGRGHFYEGRGMTIMTDAIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTM 140

Query: 383 PGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGP 562
           PG     P+ G N+D+FGERF  L++AYD   R   +++A +  E   + EGV+    GP
Sbjct: 141 PG----TPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKE--EGFPLTEGVFVSYPGP 194

Query: 563 AYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEKKANHL 742
            +ET +E +MM+++GGD  GMS   EVI ARH  + V+ +S ITN  + E  S+ K +H 
Sbjct: 195 NFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITN--MAEGLSDVKLSHA 252

Query: 743 EVLAVAKERAQIMQTLI 793
           + LA A+   Q    LI
Sbjct: 253 QTLAAAELSKQNFINLI 269

>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 268

 Score =  158 bits (399), Expect = 2e-38
 Identities = 91/235 (38%), Positives = 131/235 (55%)
 Frame = +2

Query: 101 KQIIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLN 280
           + + P +E+ GF     AGH G L+   +G   ++++ GR HPYEG+  +  V PVR   
Sbjct: 49  RAVFPQAELPGFITPNAAGHTGELLSVRIGAHRVLVLAGRIHPYEGHDLRHVVHPVRTAC 108

Query: 281 LIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSD 460
             G + ++LTNAAGGL     VG +++I DH+N   L   +PL G +       F+ L++
Sbjct: 109 AAGARIIVLTNAAGGLRADMAVGQLVLISDHLN---LTTRSPLVGTH-------FVDLTN 158

Query: 461 AYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHE 640
           AY   LR  A +       D  + EGVYA Q GP YET +E +M++MLG D  GMST HE
Sbjct: 159 AYTTRLRKLASD------TDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHE 212

Query: 641 VIVARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 805
            I AR  G  VLG+SL+TN  +    + K  NH EVLA     A  + +L++ ++
Sbjct: 213 TIAARAAGAEVLGVSLVTN--LAAGITGKPLNHAEVLAAGTASANRIGSLLADII 265

>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
 sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 268

 Score =  154 bits (388), Expect = 4e-37
 Identities = 92/233 (39%), Positives = 132/233 (56%)
 Frame = +2

Query: 107 IIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLI 286
           ++P +E+ GF   T AGHAG L+   +G   ++++ GR H YEG+  +  V PVR     
Sbjct: 51  VLPQAELPGFVPPTAAGHAGELLSVPIGAHRVLVLAGRIHAYEGHDLRYVVHPVRAARAA 110

Query: 287 GVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAY 466
           G + ++LTNAAGGL    +VG  ++I DH+N   L   +PL G      GE F+ L+DAY
Sbjct: 111 GAQIMVLTNAAGGLRADLQVGQPVLISDHLN---LTARSPLVG------GE-FVDLTDAY 160

Query: 467 DKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVI 646
              LR  A++       D ++ EGVYA   GP YET +E +M++ LG D  GMST HE I
Sbjct: 161 SPRLRELARQ------SDPQLAEGVYAGLPGPHYETPAEIRMLQTLGADLVGMSTVHETI 214

Query: 647 VARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 805
            AR  G  VLG+SL+TN  +    + +  +H EVLA     A  M  L++ ++
Sbjct: 215 AARAAGAEVLGVSLVTN--LAAGITGEPLSHAEVLAAGAASATRMGALLADVI 265

>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
          Length = 282

 Score =  122 bits (306), Expect = 1e-27
 Identities = 84/238 (35%), Positives = 121/238 (50%), Gaps = 5/238 (2%)
 Frame = +2

Query: 110 IPYSEINGFPKSTVAGHAG---NLVFGHLGG--KEIVLMQGRFHPYEGYTTQTCVIPVRV 274
           +P  EI GF    VAGH     ++      G  +  +++  R H YEG   +  V  VR 
Sbjct: 62  VPTHEIPGFSAPAVAGHLSVTRSIRVERADGSVRHALVLGSRTHLYEGKGVRAVVHGVRT 121

Query: 275 LNLIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPL 454
               G + L+LTN  GGLN  +  G  +++ DHIN   L   +PL GP        F+ L
Sbjct: 122 AAATGAETLILTNGCGGLNQEWGAGTPVLLSDHIN---LTARSPLEGPT-------FVDL 171

Query: 455 SDAYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTT 634
           +D Y   LR    E+A+++  D  + EGVYA   GP YET +E +M  +LG D  GMSTT
Sbjct: 172 TDVYSPRLR----ELAHRV--DPTLPEGVYAQFPGPHYETPAEVRMAGILGADLVGMSTT 225

Query: 635 HEVIVARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLVE 808
            E I ARH G+ VLG+SL+TN  +    S    +H EV+   +     +  L++ + +
Sbjct: 226 LEAIAARHCGLEVLGVSLVTN--LAAGISPTPLSHAEVIEAGQAAGPRISALLADIAK 281

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 110,246,884
Number of Sequences: 369166
Number of extensions: 2369249
Number of successful extensions: 5398
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5383
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9318621220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00707

  1. Dr_sW_018_H16
  2. Dr_sW_013_F22
  3. Dr_sW_012_A04