Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_F22
(592 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosi... 191 1e-48
sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosi... 187 2e-47
sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosi... 186 3e-47
sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosi... 146 4e-35
sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP... 137 2e-32
sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP... 126 5e-29
sp|P45563|XAPA_ECOLI Xanthosine phosphorylase 109 4e-24
sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosi... 103 4e-22
sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosi... 98 2e-20
sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosi... 95 1e-19
>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 191 bits (485), Expect = 1e-48
Identities = 88/168 (52%), Positives = 125/168 (74%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGGLNP ++VGD+M+I+DHIN PG CG NPL GPN+++FG RF +SDAYD+++R KA
Sbjct: 117 AGGLNPNFEVGDIMLIRDHINLPGFCGQNPLRGPNDERFGVRFPAMSDAYDRDMRQKAFT 176
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
++GE +++ EG Y M GP +ETV+E +++KMLG DA GMST EVIVARH G+ V
Sbjct: 177 AWKQMGEQRKLQEGTYVMLAGPNFETVAESRLLKMLGADAVGMSTVPEVIVARHCGLRVF 236
Query: 362 GISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLVEILQI 505
G SLITN+ V+++++ +KANH+EVL K AQ ++ +S L+E + +
Sbjct: 237 GFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLERFVSILMESIPL 284
>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 187 bits (475), Expect = 2e-47
Identities = 85/163 (52%), Positives = 122/163 (74%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGGLNP ++VGD+M+I+DHIN PG G NPL GPN+++FG+RF +SDAYD+ +R +A
Sbjct: 117 AGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALS 176
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
++GE + + EG Y M GP++ETV+EC++++ LG DA GMST EVIVARH G+ V
Sbjct: 177 TWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVF 236
Query: 362 GISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 490
G SLITN+ +++++S +KANH EVLA K+ AQ ++ +S L+
Sbjct: 237 GFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILM 279
>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 186 bits (473), Expect = 3e-47
Identities = 86/168 (51%), Positives = 122/168 (72%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGGLNP ++VGD+M+I+DHIN PG G NPL GPNE++FG RF +SDAYD+++R KA
Sbjct: 117 AGGLNPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHS 176
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
++GE + + EG Y M GP +ETV+EC++++ LG DA GMST EVIVARH G+ V
Sbjct: 177 TWKQMGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVF 236
Query: 362 GISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLVEILQI 505
G SLITN+ +++++S+ KANH EVL K+ AQ ++ +S L+ + +
Sbjct: 237 GFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASIPV 284
>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 311
Score = 146 bits (368), Expect = 4e-35
Identities = 81/174 (46%), Positives = 106/174 (60%), Gaps = 11/174 (6%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGG+N Y+ D+M I DH+N PGL G +PL GPN D+ G RF+ LSDAYD LR +
Sbjct: 135 AGGINAKYQACDLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAYDLELRKLLFK 194
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
+L + +HEG Y GP +ET +E KM++MLGGDA GMST EVIVARH G VL
Sbjct: 195 KWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVPEVIVARHCGWRVL 254
Query: 362 GISLITNECVLEHDS-----------EKKANHLEVLAVAKERAQIMQTLISKLV 490
+SLITN CV++ + + KA H EVL K + +Q LI+ ++
Sbjct: 255 ALSLITNTCVVDSPASALDESPVPLEKGKATHAEVLENGKIASNDVQNLIAAVM 308
>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 274
Score = 137 bits (346), Expect = 2e-32
Identities = 72/127 (56%), Positives = 89/127 (70%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGG+N ++ GD+MII DHIN G NPL GPN+ G RF +S+AY K LR AK+
Sbjct: 113 AGGVNESFEPGDLMIISDHINN---MGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKD 169
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
+AN +G RV EGVY GPAYET +E +M++++GGDA GMST EVIVARH GM VL
Sbjct: 170 VANDIG--LRVREGVYVANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVL 227
Query: 362 GISLITN 382
GIS I+N
Sbjct: 228 GISCISN 234
>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 271
Score = 126 bits (316), Expect = 5e-29
Identities = 72/150 (48%), Positives = 94/150 (62%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGG+N ++ GD+MII DHINF G NPL GPNE FG RF +S AYDK+L A++
Sbjct: 112 AGGVNTEFRAGDLMIITDHINFMGT---NPLIGPNEADFGARFPDMSSAYDKDLSSLAEK 168
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
IA L + +GVY GP+YET +E + ++ +G DA GMST EVIVA H GM VL
Sbjct: 169 IAKDLNIP--IQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVL 226
Query: 362 GISLITNECVLEHDSEKKANHLEVLAVAKE 451
GIS I+N D + +H EV+ V ++
Sbjct: 227 GISCISNAAAGILD--QPLSHDEVMEVTEK 254
>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
Length = 277
Score = 109 bits (273), Expect = 4e-24
Identities = 65/160 (40%), Positives = 93/160 (58%), Gaps = 1/160 (0%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHIN-FPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAK 178
AG L P G ++ +KDHIN PG P+ G N+D+FGERF L++AYD R +
Sbjct: 118 AGSLRPEVGAGSLVALKDHINTMPG----TPMVGLNDDRFGERFFSLANAYDAEYRALLQ 173
Query: 179 EIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSV 358
++A + G + EGV+ GP +ET +E +MM+++GGD GMS EVI ARH + V
Sbjct: 174 KVAKEEGFP--LTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKV 231
Query: 359 LGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLI 478
+ +S ITN + E S+ K +H + LA A+ Q LI
Sbjct: 232 VAVSAITN--MAEGLSDVKLSHAQTLAAAELSKQNFINLI 269
>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 103 bits (256), Expect = 4e-22
Identities = 65/163 (39%), Positives = 90/163 (55%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGGL VG +++I DH+N L +PL G + F+ L++AY LR A +
Sbjct: 121 AGGLRADMAVGQLVLISDHLN---LTTRSPLVGTH-------FVDLTNAYTTRLRKLASD 170
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
D + EGVYA Q GP YET +E +M++MLG D GMST HE I AR G VL
Sbjct: 171 T------DPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVL 224
Query: 362 GISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 490
G+SL+TN + + K NH EVLA A + +L++ ++
Sbjct: 225 GVSLVTN--LAAGITGKPLNHAEVLAAGTASANRIGSLLADII 265
>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 97.8 bits (242), Expect = 2e-20
Identities = 65/163 (39%), Positives = 90/163 (55%)
Frame = +2
Query: 2 AGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKE 181
AGGL +VG ++I DH+N L +PL G GE F+ L+DAY LR A++
Sbjct: 121 AGGLRADLQVGQPVLISDHLN---LTARSPLVG------GE-FVDLTDAYSPRLRELARQ 170
Query: 182 IANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVL 361
D ++ EGVYA GP YET +E +M++ LG D GMST HE I AR G VL
Sbjct: 171 ------SDPQLAEGVYAGLPGPHYETPAEIRMLQTLGADLVGMSTVHETIAARAAGAEVL 224
Query: 362 GISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 490
G+SL+TN + + + +H EVLA A M L++ ++
Sbjct: 225 GVSLVTN--LAAGITGEPLSHAEVLAAGAASATRMGALLADVI 265
>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 282
Score = 94.7 bits (234), Expect = 1e-19
Identities = 61/163 (37%), Positives = 88/163 (53%)
Frame = +2
Query: 5 GGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEI 184
GGLN + G +++ DHIN L +PL GP F+ L+D Y LR E+
Sbjct: 137 GGLNQEWGAGTPVLLSDHIN---LTARSPLEGPT-------FVDLTDVYSPRLR----EL 182
Query: 185 ANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLG 364
A+++ D + EGVYA GP YET +E +M +LG D GMSTT E I ARH G+ VLG
Sbjct: 183 AHRV--DPTLPEGVYAQFPGPHYETPAEVRMAGILGADLVGMSTTLEAIAARHCGLEVLG 240
Query: 365 ISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLVE 493
+SL+TN + S +H EV+ + + L++ + +
Sbjct: 241 VSLVTN--LAAGISPTPLSHAEVIEAGQAAGPRISALLADIAK 281
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,608,175
Number of Sequences: 369166
Number of extensions: 1276431
Number of successful extensions: 2964
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2870
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2951
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4455160255
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)