Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_H16
(910 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosi... 290 3e-78
sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosi... 288 1e-77
sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosi... 283 6e-76
sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP... 221 2e-57
sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosi... 218 2e-56
sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP... 199 7e-51
sp|P45563|XAPA_ECOLI Xanthosine phosphorylase 174 4e-43
sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosi... 158 2e-38
sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosi... 154 4e-37
sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosi... 122 1e-27
>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 290 bits (743), Expect = 3e-78
Identities = 140/271 (51%), Positives = 192/271 (70%)
Frame = +2
Query: 8 ANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHL 187
A +LL T++RP++AVIC QI Y+EI FP+STV GHAG LVFG L
Sbjct: 14 AKWLLQHTEYRPQVAVICGSGLGGLTAHLKEAQIFDYNEIPNFPQSTVQGHAGRLVFGLL 73
Query: 188 GGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIK 367
G+ V+MQGRFH YEGY+ PVRV +L+GV+ L++TNAAGGLNP ++VGD+M+I+
Sbjct: 74 NGRCCVMMQGRFHMYEGYSLSKVTFPVRVFHLLGVETLVVTNAAGGLNPNFEVGDIMLIR 133
Query: 368 DHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYA 547
DHIN PG CG NPL GPN+++FG RF +SDAYD+++R KA ++GE +++ EG Y
Sbjct: 134 DHINLPGFCGQNPLRGPNDERFGVRFPAMSDAYDRDMRQKAFTAWKQMGEQRKLQEGTYV 193
Query: 548 MQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEK 727
M GP +ETV+E +++KMLG DA GMST EVIVARH G+ V G SLITN+ V+++++ +
Sbjct: 194 MLAGPNFETVAESRLLKMLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVVMDYENLE 253
Query: 728 KANHLEVLAVAKERAQIMQTLISKLVEILQI 820
KANH+EVL K AQ ++ +S L+E + +
Sbjct: 254 KANHMEVLDAGKAAAQTLERFVSILMESIPL 284
>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 288 bits (737), Expect = 1e-77
Identities = 138/266 (51%), Positives = 186/266 (69%)
Frame = +2
Query: 8 ANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHL 187
A +LL TK RP++A+IC QI YSEI FP+STV GHAG LVFG L
Sbjct: 14 AEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFL 73
Query: 188 GGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIK 367
G+ V+MQGRFH YEGY PVRV +L+GV L++TNAAGGLNP ++VGD+M+I+
Sbjct: 74 NGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIR 133
Query: 368 DHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYA 547
DHIN PG G NPL GPN+++FG+RF +SDAYD+ +R +A ++GE + + EG Y
Sbjct: 134 DHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYV 193
Query: 548 MQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEK 727
M GP++ETV+EC++++ LG DA GMST EVIVARH G+ V G SLITN+ +++++S +
Sbjct: 194 MVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLE 253
Query: 728 KANHLEVLAVAKERAQIMQTLISKLV 805
KANH EVLA K+ AQ ++ +S L+
Sbjct: 254 KANHEEVLAAGKQAAQKLEQFVSILM 279
>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 283 bits (723), Expect = 6e-76
Identities = 138/273 (50%), Positives = 186/273 (68%)
Frame = +2
Query: 2 EIANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFG 181
+ A +LL T+ RP++AVIC Q YSEI FP+STV GHAG LVFG
Sbjct: 12 DTAKWLLSHTEQRPQVAVICGSGLGGLVNKLTQAQTFDYSEIPNFPESTVPGHAGRLVFG 71
Query: 182 HLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMI 361
L G+ V+MQGRFH YEGY PVRV L+GV+ L++TNAAGGLNP ++VGD+M+
Sbjct: 72 ILNGRACVMMQGRFHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGLNPNFEVGDIML 131
Query: 362 IKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGV 541
I+DHIN PG G NPL GPNE++FG RF +SDAYD+++R KA ++GE + + EG
Sbjct: 132 IRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHSTWKQMGEQRELQEGT 191
Query: 542 YAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDS 721
Y M GP +ETV+EC++++ LG DA GMST EVIVARH G+ V G SLITN+ +++++S
Sbjct: 192 YVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYES 251
Query: 722 EKKANHLEVLAVAKERAQIMQTLISKLVEILQI 820
+ KANH EVL K+ AQ ++ +S L+ + +
Sbjct: 252 QGKANHEEVLEAGKQAAQKLEQFVSLLMASIPV 284
>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 274
Score = 221 bits (563), Expect = 2e-57
Identities = 119/232 (51%), Positives = 149/232 (64%)
Frame = +2
Query: 2 EIANFLLGKTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFG 181
+ A FL K P+I +I IPYS+I FP STV GHAG LV+G
Sbjct: 8 QAAQFLKEKFPTSPQIGLILGSGLGVLADEIEQAIKIPYSDIPNFPVSTVEGHAGQLVYG 67
Query: 182 HLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMI 361
L G +V+MQGRFH YEGY+ PVRV+ +GV+ L++TNAAGG+N ++ GD+MI
Sbjct: 68 QLEGATVVVMQGRFHYYEGYSFDKVTFPVRVMKALGVEQLIVTNAAGGVNESFEPGDLMI 127
Query: 362 IKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGV 541
I DHIN G NPL GPN+ G RF +S+AY K LR AK++AN +G RV EGV
Sbjct: 128 ISDHIN---NMGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIG--LRVREGV 182
Query: 542 YAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 697
Y GPAYET +E +M++++GGDA GMST EVIVARH GM VLGIS I+N
Sbjct: 183 YVANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISN 234
>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 311
Score = 218 bits (555), Expect = 2e-56
Identities = 118/244 (48%), Positives = 154/244 (63%), Gaps = 12/244 (4%)
Frame = +2
Query: 110 IPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLIG 289
+PY +I GF KSTV GH+G L+FG + G +VLM GR H YEG T P+RVLN +G
Sbjct: 65 VPYQDIPGFKKSTVPGHSGTLMFGSMNGSPVVLMNGRLHGYEGNTLFETTFPIRVLNHMG 124
Query: 290 -VKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAY 466
V+ L++TNAAGG+N Y+ D+M I DH+N PGL G +PL GPN D+ G RF+ LSDAY
Sbjct: 125 HVRNLIVTNAAGGINAKYQACDLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAY 184
Query: 467 DKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVI 646
D LR + +L + +HEG Y GP +ET +E KM++MLGGDA GMST EVI
Sbjct: 185 DLELRKLLFKKWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVPEVI 244
Query: 647 VARHMGMSVLGISLITNECVLEHDS-----------EKKANHLEVLAVAKERAQIMQTLI 793
VARH G VL +SLITN CV++ + + KA H EVL K + +Q LI
Sbjct: 245 VARHCGWRVLALSLITNTCVVDSPASALDESPVPLEKGKATHAEVLENGKIASNDVQNLI 304
Query: 794 SKLV 805
+ ++
Sbjct: 305 AAVM 308
>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 271
Score = 199 bits (507), Expect = 7e-51
Identities = 112/242 (46%), Positives = 147/242 (60%)
Frame = +2
Query: 41 PKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGR 220
PKI +I + Y +I FP STV GHAG LV G L G ++ MQGR
Sbjct: 20 PKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTLEGVSVIAMQGR 79
Query: 221 FHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGF 400
FH YEGY+ + PVRV+ +GV+ L++TNAAGG+N ++ GD+MII DHINF G
Sbjct: 80 FHFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIITDHINF---MGT 136
Query: 401 NPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVS 580
NPL GPNE FG RF +S AYDK+L A++IA L + + +GVY GP+YET +
Sbjct: 137 NPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDL--NIPIQKGVYTAVTGPSYETPA 194
Query: 581 ECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVA 760
E + ++ +G DA GMST EVIVA H GM VLGIS I+N D + +H EV+ V
Sbjct: 195 EVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD--QPLSHDEVMEVT 252
Query: 761 KE 766
++
Sbjct: 253 EK 254
>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
Length = 277
Score = 174 bits (440), Expect = 4e-43
Identities = 103/257 (40%), Positives = 140/257 (54%), Gaps = 1/257 (0%)
Frame = +2
Query: 26 KTKFRPKIAVICXXXXXXXXXXXXXKQIIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIV 205
K F P++A I I Y ++ GFP STV GHAG LV GHL G +V
Sbjct: 21 KPDFTPRVAFILGSGLGALADQIENAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVV 80
Query: 206 LMQGRFHPYEGYTTQTCVIPVRVLNLIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHIN-F 382
M+GR H YEG +R L+G + L TNAAG L P G ++ +KDHIN
Sbjct: 81 CMKGRGHFYEGRGMTIMTDAIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTM 140
Query: 383 PGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGP 562
PG P+ G N+D+FGERF L++AYD R +++A + E + EGV+ GP
Sbjct: 141 PG----TPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKE--EGFPLTEGVFVSYPGP 194
Query: 563 AYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVLEHDSEKKANHL 742
+ET +E +MM+++GGD GMS EVI ARH + V+ +S ITN + E S+ K +H
Sbjct: 195 NFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITN--MAEGLSDVKLSHA 252
Query: 743 EVLAVAKERAQIMQTLI 793
+ LA A+ Q LI
Sbjct: 253 QTLAAAELSKQNFINLI 269
>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 158 bits (399), Expect = 2e-38
Identities = 91/235 (38%), Positives = 131/235 (55%)
Frame = +2
Query: 101 KQIIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLN 280
+ + P +E+ GF AGH G L+ +G ++++ GR HPYEG+ + V PVR
Sbjct: 49 RAVFPQAELPGFITPNAAGHTGELLSVRIGAHRVLVLAGRIHPYEGHDLRHVVHPVRTAC 108
Query: 281 LIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSD 460
G + ++LTNAAGGL VG +++I DH+N L +PL G + F+ L++
Sbjct: 109 AAGARIIVLTNAAGGLRADMAVGQLVLISDHLN---LTTRSPLVGTH-------FVDLTN 158
Query: 461 AYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHE 640
AY LR A + D + EGVYA Q GP YET +E +M++MLG D GMST HE
Sbjct: 159 AYTTRLRKLASD------TDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHE 212
Query: 641 VIVARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 805
I AR G VLG+SL+TN + + K NH EVLA A + +L++ ++
Sbjct: 213 TIAARAAGAEVLGVSLVTN--LAAGITGKPLNHAEVLAAGTASANRIGSLLADII 265
>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 154 bits (388), Expect = 4e-37
Identities = 92/233 (39%), Positives = 132/233 (56%)
Frame = +2
Query: 107 IIPYSEINGFPKSTVAGHAGNLVFGHLGGKEIVLMQGRFHPYEGYTTQTCVIPVRVLNLI 286
++P +E+ GF T AGHAG L+ +G ++++ GR H YEG+ + V PVR
Sbjct: 51 VLPQAELPGFVPPTAAGHAGELLSVPIGAHRVLVLAGRIHAYEGHDLRYVVHPVRAARAA 110
Query: 287 GVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAY 466
G + ++LTNAAGGL +VG ++I DH+N L +PL G GE F+ L+DAY
Sbjct: 111 GAQIMVLTNAAGGLRADLQVGQPVLISDHLN---LTARSPLVG------GE-FVDLTDAY 160
Query: 467 DKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVI 646
LR A++ D ++ EGVYA GP YET +E +M++ LG D GMST HE I
Sbjct: 161 SPRLRELARQ------SDPQLAEGVYAGLPGPHYETPAEIRMLQTLGADLVGMSTVHETI 214
Query: 647 VARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLV 805
AR G VLG+SL+TN + + + +H EVLA A M L++ ++
Sbjct: 215 AARAAGAEVLGVSLVTN--LAAGITGEPLSHAEVLAAGAASATRMGALLADVI 265
>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 282
Score = 122 bits (306), Expect = 1e-27
Identities = 84/238 (35%), Positives = 121/238 (50%), Gaps = 5/238 (2%)
Frame = +2
Query: 110 IPYSEINGFPKSTVAGHAG---NLVFGHLGG--KEIVLMQGRFHPYEGYTTQTCVIPVRV 274
+P EI GF VAGH ++ G + +++ R H YEG + V VR
Sbjct: 62 VPTHEIPGFSAPAVAGHLSVTRSIRVERADGSVRHALVLGSRTHLYEGKGVRAVVHGVRT 121
Query: 275 LNLIGVKFLLLTNAAGGLNPIYKVGDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPL 454
G + L+LTN GGLN + G +++ DHIN L +PL GP F+ L
Sbjct: 122 AAATGAETLILTNGCGGLNQEWGAGTPVLLSDHIN---LTARSPLEGPT-------FVDL 171
Query: 455 SDAYDKNLRMKAKEIANKLGEDKRVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTT 634
+D Y LR E+A+++ D + EGVYA GP YET +E +M +LG D GMSTT
Sbjct: 172 TDVYSPRLR----ELAHRV--DPTLPEGVYAQFPGPHYETPAEVRMAGILGADLVGMSTT 225
Query: 635 HEVIVARHMGMSVLGISLITNECVLEHDSEKKANHLEVLAVAKERAQIMQTLISKLVE 808
E I ARH G+ VLG+SL+TN + S +H EV+ + + L++ + +
Sbjct: 226 LEAIAARHCGLEVLGVSLVTN--LAAGISPTPLSHAEVIEAGQAAGPRISALLADIAK 281
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,623,360
Number of Sequences: 369166
Number of extensions: 2355934
Number of successful extensions: 5372
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5357
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9222552960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)