Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_A04
(569 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosi... 152 7e-37
sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosi... 148 8e-36
sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosi... 148 1e-35
sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosi... 130 2e-30
sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP... 129 5e-30
sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP... 116 4e-26
sp|P45563|XAPA_ECOLI Xanthosine phosphorylase 97 4e-20
sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosi... 88 2e-17
sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosi... 86 5e-17
sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosi... 85 1e-16
>sp|P23492|PNPH_MOUSE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 152 bits (383), Expect = 7e-37
Identities = 68/124 (54%), Positives = 94/124 (75%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
GD+M+I+DHIN PG CG NPL GPN+++FG RF +SDAYD+++R KA ++GE ++
Sbjct: 127 GDIMLIRDHINLPGFCGQNPLRGPNDERFGVRFPAMSDAYDRDMRQKAFTAWKQMGEQRK 186
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECV 360
+ EG Y M GP +ETV+E +++KMLG DA GMST EVIVARH G+ V G SLITN+ V
Sbjct: 187 LQEGTYVMLAGPNFETVAESRLLKMLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVV 246
Query: 361 LEHD 372
++++
Sbjct: 247 MDYE 250
Score = 33.5 bits (75), Expect = 0.38
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +2
Query: 323 RYWEFLLLPTSVFWNMIQKKKANHLEVLAVAKERAQIMQTLISKLVEILQI 475
R + F L+ V + +KANH+EVL K AQ ++ +S L+E + +
Sbjct: 234 RVFGFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLERFVSILMESIPL 284
>sp|P00491|PNPH_HUMAN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 148 bits (374), Expect = 8e-36
Identities = 64/124 (51%), Positives = 93/124 (75%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
GD+M+I+DHIN PG G NPL GPN+++FG+RF +SDAYD+ +R +A ++GE +
Sbjct: 127 GDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRE 186
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECV 360
+ EG Y M GP++ETV+EC++++ LG DA GMST EVIVARH G+ V G SLITN+ +
Sbjct: 187 LQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVI 246
Query: 361 LEHD 372
++++
Sbjct: 247 MDYE 250
Score = 32.3 bits (72), Expect = 0.85
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +2
Query: 323 RYWEFLLLPTSVFWNMIQKKKANHLEVLAVAKERAQIMQTLISKLV 460
R + F L+ V + +KANH EVLA K+ AQ ++ +S L+
Sbjct: 234 RVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILM 279
>sp|P55859|PNPH_BOVIN Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 289
Score = 148 bits (373), Expect = 1e-35
Identities = 66/124 (53%), Positives = 92/124 (74%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
GD+M+I+DHIN PG G NPL GPNE++FG RF +SDAYD+++R KA ++GE +
Sbjct: 127 GDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHSTWKQMGEQRE 186
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECV 360
+ EG Y M GP +ETV+EC++++ LG DA GMST EVIVARH G+ V G SLITN+ +
Sbjct: 187 LQEGTYVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVI 246
Query: 361 LEHD 372
++++
Sbjct: 247 MDYE 250
Score = 31.2 bits (69), Expect = 1.9
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +2
Query: 323 RYWEFLLLPTSVFWNMIQKKKANHLEVLAVAKERAQIMQTLISKLVEILQI 475
R + F L+ V + + KANH EVL K+ AQ ++ +S L+ + +
Sbjct: 234 RVFGFSLITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASIPV 284
>sp|Q05788|PNPH_YEAST Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 311
Score = 130 bits (327), Expect = 2e-30
Identities = 66/121 (54%), Positives = 82/121 (67%)
Frame = +1
Query: 4 DMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKRV 183
D+M I DH+N PGL G +PL GPN D+ G RF+ LSDAYD LR + +L + +
Sbjct: 146 DLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAYDLELRKLLFKKWKELKIQRPL 205
Query: 184 HEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITNECVL 363
HEG Y GP +ET +E KM++MLGGDA GMST EVIVARH G VL +SLITN CV+
Sbjct: 206 HEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVPEVIVARHCGWRVLALSLITNTCVV 265
Query: 364 E 366
+
Sbjct: 266 D 266
>sp|P77834|PUNA_BACST Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 274
Score = 129 bits (324), Expect = 5e-30
Identities = 68/117 (58%), Positives = 82/117 (70%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
GD+MII DHIN G NPL GPN+ G RF +S+AY K LR AK++AN +G R
Sbjct: 123 GDLMIISDHINN---MGGNPLIGPNDSALGVRFPDMSEAYSKRLRQLAKDVANDIG--LR 177
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 351
V EGVY GPAYET +E +M++++GGDA GMST EVIVARH GM VLGIS I+N
Sbjct: 178 VREGVYVANTGPAYETPAEIRMIRVMGGDAVGMSTVPEVIVARHAGMEVLGISCISN 234
>sp|P46354|PUNA_BACSU Purine nucleoside phosphorylase I (PNP I) (PU-NPASE I) (Inosine
phosphorylase)
Length = 271
Score = 116 bits (290), Expect = 4e-26
Identities = 63/117 (53%), Positives = 77/117 (65%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
GD+MII DHINF G NPL GPNE FG RF +S AYDK+L A++IA L
Sbjct: 122 GDLMIITDHINFMGT---NPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDLNIP-- 176
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 351
+ +GVY GP+YET +E + ++ +G DA GMST EVIVA H GM VLGIS I+N
Sbjct: 177 IQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISN 233
>sp|P45563|XAPA_ECOLI Xanthosine phosphorylase
Length = 277
Score = 96.7 bits (239), Expect = 4e-20
Identities = 51/118 (43%), Positives = 74/118 (62%), Gaps = 1/118 (0%)
Frame = +1
Query: 1 GDMMIIKDHIN-FPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDK 177
G ++ +KDHIN PG P+ G N+D+FGERF L++AYD R +++A + G
Sbjct: 128 GSLVALKDHINTMPG----TPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKEEGFP- 182
Query: 178 RVHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 351
+ EGV+ GP +ET +E +MM+++GGD GMS EVI ARH + V+ +S ITN
Sbjct: 183 -LTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITN 239
>sp|P46862|PUNA_MYCLE Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 87.8 bits (216), Expect = 2e-17
Identities = 51/117 (43%), Positives = 68/117 (58%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
G +++I DH+N L +PL G + F+ L++AY LR A + D
Sbjct: 131 GQLVLISDHLN---LTTRSPLVGTH-------FVDLTNAYTTRLRKLASDT------DPT 174
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 351
+ EGVYA Q GP YET +E +M++MLG D GMST HE I AR G VLG+SL+TN
Sbjct: 175 LTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTN 231
>sp|P81989|PUNA_CELSP Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 282
Score = 86.3 bits (212), Expect = 5e-17
Identities = 52/117 (44%), Positives = 69/117 (58%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
G +++ DHIN L +PL GP F+ L+D Y LR E+A+++ D
Sbjct: 146 GTPVLLSDHIN---LTARSPLEGPT-------FVDLTDVYSPRLR----ELAHRV--DPT 189
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 351
+ EGVYA GP YET +E +M +LG D GMSTT E I ARH G+ VLG+SL+TN
Sbjct: 190 LPEGVYAQFPGPHYETPAEVRMAGILGADLVGMSTTLEAIAARHCGLEVLGVSLVTN 246
>sp|P0A539|PUNA_MYCBO Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
sp|P0A538|PUNA_MYCTU Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP)
Length = 268
Score = 84.7 bits (208), Expect = 1e-16
Identities = 52/117 (44%), Positives = 68/117 (58%)
Frame = +1
Query: 1 GDMMIIKDHINFPGLCGFNPLTGPNEDKFGERFIPLSDAYDKNLRMKAKEIANKLGEDKR 180
G ++I DH+N L +PL G GE F+ L+DAY LR A++ D +
Sbjct: 131 GQPVLISDHLN---LTARSPLVG------GE-FVDLTDAYSPRLRELARQ------SDPQ 174
Query: 181 VHEGVYAMQLGPAYETVSECKMMKMLGGDACGMSTTHEVIVARHMGMSVLGISLITN 351
+ EGVYA GP YET +E +M++ LG D GMST HE I AR G VLG+SL+TN
Sbjct: 175 LAEGVYAGLPGPHYETPAEIRMLQTLGADLVGMSTVHETIAARAAGAEVLGVSLVTN 231
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,924,430
Number of Sequences: 369166
Number of extensions: 1209135
Number of successful extensions: 2838
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2832
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4177115900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)