Planaria EST Database

DrC_00680

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00680
         (1328 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...    84   9e-16
sp|Q9P286|PAK7_HUMAN  Serine/threonine-protein kinase PAK 7 ...    84   9e-16
sp|Q8C015|PAK7_MOUSE  Serine/threonine-protein kinase PAK 7 ...    84   9e-16
sp|O22042|M3K3_ARATH  Mitogen-activated protein kinase kinas...    82   3e-15
sp|Q5A649|ATG1_CANAL  Serine/threonine-protein kinase ATG1 (...    81   6e-15
sp|Q99683|M3K5_HUMAN  Mitogen-activated protein kinase kinas...    81   8e-15
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...    80   1e-14
sp|O95747|OXSR1_HUMAN  Serine/threonine-protein kinase OSR1 ...    79   2e-14
sp|Q863I2|OXSR1_PIG  Serine/threonine-protein kinase OSR1 (O...    79   2e-14
sp|Q6P9R2|OXSR1_MOUSE  Serine/threonine-protein kinase OSR1 ...    79   2e-14

>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
            NPK1-related protein kinase 2)
          Length = 651

 Score = 84.0 bits (206), Expect = 9e-16
 Identities = 54/138 (39%), Positives = 74/138 (53%), Gaps = 4/138 (2%)
 Frame = +2

Query: 905  GANILITKQDCIKIVDFGWSRF---LQTVSRQEKKKKTFCGTTNYMSPEVINDEPYGFPA 1075
            GANIL+  Q CIK+ DFG S+    L T+S  +  K    GT  +M+PEVI    + F A
Sbjct: 199  GANILVDNQGCIKLADFGASKQVAELATISGAKSMK----GTPYWMAPEVILQTGHSFSA 254

Query: 1076 DIWSVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQLENSKILEK-DEETKQFIDLMTK 1252
            DIWSVG TV EM TG+ P+     EIA    I  TK   +  I +    +   F+    +
Sbjct: 255  DIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTK--SHPPIPDNISSDANDFLLKCLQ 312

Query: 1253 ADPNERKTADQLLNYHYL 1306
             +PN R TA +LL + ++
Sbjct: 313  QEPNLRPTASELLKHPFV 330


 Score = 41.6 bits (96), Expect = 0.005
 Identities = 21/50 (42%), Positives = 28/50 (56%)
 Frame = +3

Query: 723 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSRIIHRDIKSIYFL 872
           GSIS    L  +   P   ++ YT Q+L GL++LH   I+HRDIK    L
Sbjct: 156 GSISSL--LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANIL 203

>sp|Q9P286|PAK7_HUMAN Serine/threonine-protein kinase PAK 7 (p21-activated kinase 7)
            (PAK-7) (PAK-5)
          Length = 719

 Score = 84.0 bits (206), Expect = 9e-16
 Identities = 48/133 (36%), Positives = 75/133 (56%)
 Frame = +2

Query: 911  NILITKQDCIKIVDFGWSRFLQTVSRQEKKKKTFCGTTNYMSPEVINDEPYGFPADIWSV 1090
            +IL+T    IK+ DFG   F   VS++  K+K+  GT  +M+PEVI+  PYG   DIWS+
Sbjct: 573  SILLTSDGRIKLSDFG---FCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSL 629

Query: 1091 GATVFEMATGRPPYHSVKNEIAVGVKIANTKQLENSKILEKDEETKQFIDLMTKADPNER 1270
            G  V EM  G PPY + +  +    +I ++       + +     + F+DLM   +P++R
Sbjct: 630  GIMVIEMIDGEPPYFN-EPPLQAMRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQR 688

Query: 1271 KTADQLLNYHYLK 1309
             TA +LL + +LK
Sbjct: 689  ATAQELLGHPFLK 701

>sp|Q8C015|PAK7_MOUSE Serine/threonine-protein kinase PAK 7 (p21-activated kinase 7)
            (PAK-7)
          Length = 719

 Score = 84.0 bits (206), Expect = 9e-16
 Identities = 48/133 (36%), Positives = 75/133 (56%)
 Frame = +2

Query: 911  NILITKQDCIKIVDFGWSRFLQTVSRQEKKKKTFCGTTNYMSPEVINDEPYGFPADIWSV 1090
            +IL+T    IK+ DFG   F   VS++  K+K+  GT  +M+PEVI+  PYG   DIWS+
Sbjct: 573  SILLTSDGRIKLSDFG---FCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSL 629

Query: 1091 GATVFEMATGRPPYHSVKNEIAVGVKIANTKQLENSKILEKDEETKQFIDLMTKADPNER 1270
            G  V EM  G PPY + +  +    +I ++       + +     + F+DLM   +P++R
Sbjct: 630  GIMVIEMIDGEPPYFN-EPPLQAMRRIRDSLPPRVKDLHKVSSMLRGFLDLMLVREPSQR 688

Query: 1271 KTADQLLNYHYLK 1309
             TA +LL + +LK
Sbjct: 689  ATAQELLGHPFLK 701

>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
            NPK1-related protein kinase 3)
          Length = 651

 Score = 82.0 bits (201), Expect = 3e-15
 Identities = 53/138 (38%), Positives = 76/138 (55%), Gaps = 4/138 (2%)
 Frame = +2

Query: 905  GANILITKQDCIKIVDFGWSRF---LQTVSRQEKKKKTFCGTTNYMSPEVINDEPYGFPA 1075
            GANIL+  + CI++ DFG S+    L TV+  +  K    GT  +M+PEVI    + F A
Sbjct: 199  GANILVDNKGCIRLADFGASKKVVELATVNGAKSMK----GTPYWMAPEVILQTGHSFSA 254

Query: 1076 DIWSVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQLENSKILEK-DEETKQFIDLMTK 1252
            DIWSVG TV EMATG+PP+     + A  + I  TK   +  I E    E K F+     
Sbjct: 255  DIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTK--AHPPIPEDLSPEAKDFLMKCLH 312

Query: 1253 ADPNERKTADQLLNYHYL 1306
             +P+ R +A +LL + ++
Sbjct: 313  KEPSLRLSATELLQHPFV 330


 Score = 43.1 bits (100), Expect = 0.002
 Identities = 23/50 (46%), Positives = 30/50 (60%)
 Frame = +3

Query: 723 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSRIIHRDIKSIYFL 872
           GSIS    L  +   P   I +YTKQ+L GL++LH + I+HRDIK    L
Sbjct: 156 GSISSL--LEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANIL 203

>sp|Q5A649|ATG1_CANAL Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
          Length = 834

 Score = 81.3 bits (199), Expect = 6e-15
 Identities = 44/120 (36%), Positives = 70/120 (58%), Gaps = 1/120 (0%)
 Frame = +2

Query: 938  IKIVDFGWSRFLQTVSRQEKKKKTFCGTTNYMSPEVINDEPYGFPADIWSVGATVFEMAT 1117
            +KI DFG++RFL + S  E    T CG+  YM+PE++  E Y   AD+WSVGA ++EM  
Sbjct: 236  LKIADFGFARFLPSTSMAE----TLCGSPLYMAPEILRYEKYNAKADLWSVGAVLYEMTV 291

Query: 1118 GRPPYHSVKN-EIAVGVKIANTKQLENSKILEKDEETKQFIDLMTKADPNERKTADQLLN 1294
            G+PP+ +  + E+   ++ AN K ++     +  E  KQ I  + K +P ER + ++  N
Sbjct: 292  GKPPFKAGNHIELLKNIEKANDK-IKFPSAAQVPEPLKQLIRSLLKYNPTERISFNEFFN 350

>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
            kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
            signal-regulating kinase 1) (ASK-1)
          Length = 1374

 Score = 80.9 bits (198), Expect = 8e-15
 Identities = 55/139 (39%), Positives = 78/139 (56%), Gaps = 4/139 (2%)
 Frame = +2

Query: 905  GANILI-TKQDCIKIVDFGWSRFLQTVSRQEKKKKTFCGTTNYMSPEVINDEP--YGFPA 1075
            G N+LI T    +KI DFG S+ L  ++      +TF GT  YM+PE+I+  P  YG  A
Sbjct: 806  GDNVLINTYSGVLKISDFGTSKRLAGIN---PCTETFTGTLQYMAPEIIDKGPRGYGKAA 862

Query: 1076 DIWSVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQLENSKILEK-DEETKQFIDLMTK 1252
            DIWS+G T+ EMATG+PP++ +    A   K+   K   + +I E    E K FI    +
Sbjct: 863  DIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFK--VHPEIPESMSAEAKAFILKCFE 920

Query: 1253 ADPNERKTADQLLNYHYLK 1309
             DP++R  A+ LL   +LK
Sbjct: 921  PDPDKRACANDLLVDEFLK 939


 Score = 43.9 bits (102), Expect = 0.001
 Identities = 19/28 (67%), Positives = 24/28 (85%)
 Frame = +3

Query: 774 ETIKLYTKQILCGLKFLHQSRIIHRDIK 857
           +TI  YTKQIL GLK+LH ++I+HRDIK
Sbjct: 778 QTIGFYTKQILEGLKYLHDNQIVHRDIK 805

>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
            NPK1-related protein kinase 1)
          Length = 666

 Score = 80.5 bits (197), Expect = 1e-14
 Identities = 50/135 (37%), Positives = 73/135 (54%), Gaps = 1/135 (0%)
 Frame = +2

Query: 905  GANILITKQDCIKIVDFGWSRFLQTVSRQEKKKKTFCGTTNYMSPEVINDEPYGFPADIW 1084
            GANIL+  + CIK+ DFG S+ +  ++     K +  GT  +M+PEVI    + F ADIW
Sbjct: 200  GANILVDNKGCIKLADFGASKQVAELATMTGAK-SMKGTPYWMAPEVILQTGHSFSADIW 258

Query: 1085 SVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQLENSKILEK-DEETKQFIDLMTKADP 1261
            SVG TV EM TG+ P+     E+A    I  TK   +  I +    + K F+    +  P
Sbjct: 259  SVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTK--SHPPIPDTLSSDAKDFLLKCLQEVP 316

Query: 1262 NERKTADQLLNYHYL 1306
            N R TA +LL + ++
Sbjct: 317  NLRPTASELLKHPFV 331


 Score = 45.4 bits (106), Expect = 4e-04
 Identities = 22/50 (44%), Positives = 30/50 (60%)
 Frame = +3

Query: 723 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSRIIHRDIKSIYFL 872
           GSIS    L  + P P   ++ YT+Q+L GL++LH   I+HRDIK    L
Sbjct: 157 GSISSL--LEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANIL 204

>sp|O95747|OXSR1_HUMAN Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
            protein)
          Length = 527

 Score = 79.3 bits (194), Expect = 2e-14
 Identities = 49/142 (34%), Positives = 77/142 (54%), Gaps = 9/142 (6%)
 Frame = +2

Query: 911  NILITKQDCIKIVDFGWSRFLQTVS--RQEKKKKTFCGTTNYMSPEVIND-EPYGFPADI 1081
            NIL+ +   ++I DFG S FL T     + K +KTF GT  +M+PEV+     Y F ADI
Sbjct: 151  NILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADI 210

Query: 1082 WSVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQ-----LENSKILEK-DEETKQFIDL 1243
            WS G T  E+ATG  PYH       + + + N        +++ ++L+K  +  ++ I L
Sbjct: 211  WSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISL 270

Query: 1244 MTKADPNERKTADQLLNYHYLK 1309
              + DP +R TA +LL + + +
Sbjct: 271  CLQKDPEKRPTAAELLRHKFFQ 292


 Score = 30.8 bits (68), Expect = 9.2
 Identities = 13/32 (40%), Positives = 22/32 (68%)
 Frame = +3

Query: 765 LPVETIKLYTKQILCGLKFLHQSRIIHRDIKS 860
           L   TI    +++L GL++LH++  IHRD+K+
Sbjct: 118 LDESTIATILREVLEGLEYLHKNGQIHRDVKA 149

>sp|Q863I2|OXSR1_PIG Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
            protein)
          Length = 529

 Score = 79.3 bits (194), Expect = 2e-14
 Identities = 49/142 (34%), Positives = 77/142 (54%), Gaps = 9/142 (6%)
 Frame = +2

Query: 911  NILITKQDCIKIVDFGWSRFLQTVS--RQEKKKKTFCGTTNYMSPEVIND-EPYGFPADI 1081
            NIL+ +   ++I DFG S FL T     + K +KTF GT  +M+PEV+     Y F ADI
Sbjct: 151  NILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADI 210

Query: 1082 WSVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQ-----LENSKILEK-DEETKQFIDL 1243
            WS G T  E+ATG  PYH       + + + N        +++ ++L+K  +  ++ I L
Sbjct: 211  WSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISL 270

Query: 1244 MTKADPNERKTADQLLNYHYLK 1309
              + DP +R TA +LL + + +
Sbjct: 271  CLQKDPEKRPTAAELLRHKFFQ 292

>sp|Q6P9R2|OXSR1_MOUSE Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
            protein)
          Length = 527

 Score = 79.3 bits (194), Expect = 2e-14
 Identities = 49/142 (34%), Positives = 77/142 (54%), Gaps = 9/142 (6%)
 Frame = +2

Query: 911  NILITKQDCIKIVDFGWSRFLQTVS--RQEKKKKTFCGTTNYMSPEVIND-EPYGFPADI 1081
            NIL+ +   ++I DFG S FL T     + K +KTF GT  +M+PEV+     Y F ADI
Sbjct: 151  NILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADI 210

Query: 1082 WSVGATVFEMATGRPPYHSVKNEIAVGVKIANTKQ-----LENSKILEK-DEETKQFIDL 1243
            WS G T  E+ATG  PYH       + + + N        +++ ++L+K  +  ++ I L
Sbjct: 211  WSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISL 270

Query: 1244 MTKADPNERKTADQLLNYHYLK 1309
              + DP +R TA +LL + + +
Sbjct: 271  CLQKDPEKRPTAAELLRHKFFQ 292

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 131,542,110
Number of Sequences: 369166
Number of extensions: 2451623
Number of successful extensions: 9315
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8427
length of database: 68,354,980
effective HSP length: 114
effective length of database: 47,295,190
effective search space used: 15512822320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00680

  1. Dr_sW_017_N14
  2. Dr_sW_021_P09
  3. Dr_sW_002_I24