Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_P09
(865 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 76 1e-13
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 75 3e-13
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 74 5e-13
sp|Q9Y7T4|ATG1_SCHPO Serine/threonine-protein kinase atg1 (... 73 1e-12
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 73 1e-12
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 72 2e-12
sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinas... 72 2e-12
sp|P53351|PLK2_MOUSE Serine/threonine-protein kinase PLK2 (... 72 3e-12
sp|Q9NYY3|PLK2_HUMAN Serine/threonine-protein kinase PLK2 (... 72 3e-12
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 72 3e-12
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 76.3 bits (186), Expect = 1e-13
Identities = 39/84 (46%), Positives = 52/84 (61%)
Frame = +1
Query: 610 GANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIW 789
GANIL+ Q CIK+ DFG S+ + ++ + K + GT +M+PEVI + F ADIW
Sbjct: 199 GANILVDNQGCIKLADFGASKQVAELATISGAK-SMKGTPYWMAPEVILQTGHSFSADIW 257
Query: 790 SVGATVFEMATGRPPYHSVKNEIA 861
SVG TV EM TG+ P+ EIA
Sbjct: 258 SVGCTVIEMVTGKAPWSQQYKEIA 281
Score = 41.2 bits (95), Expect = 0.004
Identities = 21/50 (42%), Positives = 28/50 (56%)
Frame = +2
Query: 428 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSNIIHRDIKSIYFL 577
GSIS L + P ++ YT Q+L GL++LH I+HRDIK L
Sbjct: 156 GSISSL--LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANIL 203
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 74.7 bits (182), Expect = 3e-13
Identities = 37/84 (44%), Positives = 53/84 (63%)
Frame = +1
Query: 610 GANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIW 789
GANIL+ + CI++ DFG S+ + ++ + K + GT +M+PEVI + F ADIW
Sbjct: 199 GANILVDNKGCIRLADFGASKKVVELATVNGAK-SMKGTPYWMAPEVILQTGHSFSADIW 257
Query: 790 SVGATVFEMATGRPPYHSVKNEIA 861
SVG TV EMATG+PP+ + A
Sbjct: 258 SVGCTVIEMATGKPPWSEQYQQFA 281
Score = 43.9 bits (102), Expect = 6e-04
Identities = 23/50 (46%), Positives = 30/50 (60%)
Frame = +2
Query: 428 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSNIIHRDIKSIYFL 577
GSIS L + P I +YTKQ+L GL++LH + I+HRDIK L
Sbjct: 156 GSISSL--LEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANIL 203
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 73.9 bits (180), Expect = 5e-13
Identities = 37/84 (44%), Positives = 52/84 (61%)
Frame = +1
Query: 610 GANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIW 789
GANIL+ + CIK+ DFG S+ + ++ + K + GT +M+PEVI + F ADIW
Sbjct: 200 GANILVDNKGCIKLADFGASKQVAELATMTGAK-SMKGTPYWMAPEVILQTGHSFSADIW 258
Query: 790 SVGATVFEMATGRPPYHSVKNEIA 861
SVG TV EM TG+ P+ E+A
Sbjct: 259 SVGCTVIEMVTGKAPWSQQYKEVA 282
Score = 45.1 bits (105), Expect = 3e-04
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = +2
Query: 428 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSNIIHRDIKSIYFL 577
GSIS L + P P ++ YT+Q+L GL++LH I+HRDIK L
Sbjct: 157 GSISSL--LEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANIL 204
>sp|Q9Y7T4|ATG1_SCHPO Serine/threonine-protein kinase atg1 (Autophagy-related protein 1)
Length = 830
Score = 72.8 bits (177), Expect = 1e-12
Identities = 33/69 (47%), Positives = 46/69 (66%)
Frame = +1
Query: 631 KQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIWSVGATVF 810
K +K+ DFG +R+LQT S E T CG+ YM+PE++ E Y AD+WSVGA ++
Sbjct: 185 KLPMLKLADFGFARYLQTSSMAE----TLCGSPLYMAPEILRYEKYDAKADLWSVGAVLY 240
Query: 811 EMATGRPPY 837
EMA G+PP+
Sbjct: 241 EMAVGKPPF 249
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 72.8 bits (177), Expect = 1e-12
Identities = 39/76 (51%), Positives = 47/76 (61%)
Frame = +1
Query: 610 GANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIW 789
GANIL IK+ DFG S+ LQT+ K+ GT +MSPEVI+ E YG ADIW
Sbjct: 486 GANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIW 545
Query: 790 SVGATVFEMATGRPPY 837
SV TV EM T +PP+
Sbjct: 546 SVACTVVEMLTEKPPW 561
Score = 38.9 bits (89), Expect = 0.019
Identities = 21/50 (42%), Positives = 29/50 (58%)
Frame = +2
Query: 428 GSISEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSNIIHRDIKSIYFL 577
GSI ++ L Y L + YT+QIL G+ +LH + I+HRDIK L
Sbjct: 443 GSIKDQ--LKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANIL 490
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 72.4 bits (176), Expect = 2e-12
Identities = 36/76 (47%), Positives = 48/76 (63%)
Frame = +1
Query: 610 GANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIW 789
GANIL +K+ DFG S+ LQT+ ++ GT +MSPEVI+ E YG AD+W
Sbjct: 492 GANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVW 551
Query: 790 SVGATVFEMATGRPPY 837
S+G TV EM T +PP+
Sbjct: 552 SLGCTVVEMLTEKPPW 567
Score = 39.7 bits (91), Expect = 0.011
Identities = 20/47 (42%), Positives = 26/47 (55%)
Frame = +2
Query: 437 SEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSNIIHRDIKSIYFL 577
S K L Y L + YT+QIL G+ +LH + I+HRDIK L
Sbjct: 450 SVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANIL 496
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
Length = 1011
Score = 72.4 bits (176), Expect = 2e-12
Identities = 40/82 (48%), Positives = 52/82 (63%), Gaps = 3/82 (3%)
Frame = +1
Query: 610 GANILI-TKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVIND--ESYGFPA 780
G N+LI T +KI DFG S+ L ++ + TF GT YM+PE+I+ YG A
Sbjct: 497 GDNVLINTFSGLLKISDFGTSKRLAGITPCTE---TFTGTLQYMAPEIIDQGPRGYGKAA 553
Query: 781 DIWSVGATVFEMATGRPPYHSV 846
DIWS+G TV EMATGRPP+H +
Sbjct: 554 DIWSLGCTVIEMATGRPPFHEL 575
Score = 40.4 bits (93), Expect = 0.006
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +2
Query: 482 TIKLYTKQILCGLKFLHQSNIIHRDIK 562
TI YT+QIL GL +LH ++I+HRDIK
Sbjct: 470 TISFYTRQILQGLGYLHDNHIVHRDIK 496
>sp|P53351|PLK2_MOUSE Serine/threonine-protein kinase PLK2 (Polo-like kinase 1) (PLK-2)
(Serine/threonine-protein kinase SNK) (Serum inducible
kinase)
Length = 682
Score = 71.6 bits (174), Expect = 3e-12
Identities = 42/137 (30%), Positives = 75/137 (54%), Gaps = 2/137 (1%)
Frame = +1
Query: 439 RENFIKQLSTSSSGNYQVVYKTNSMWSEISPPIKYYSQRY*KHLFF--KSSLFDFLI*IG 612
+EN L S + + K + +E P ++YY ++ L + + + + +G
Sbjct: 149 KENIYILLEYCSRRSMAHILKARKVLTE--PEVRYYLRQIVSGLKYLHEQEILHRDLKLG 206
Query: 613 ANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIWS 792
N I + +K+ DFGL+ L+ LE +++T CGT NY+SPEV+N + +G +DIW+
Sbjct: 207 -NFFINEAMELKVGDFGLAARLEP---LEHRRRTICGTPNYLSPEVLNKQGHGCESDIWA 262
Query: 793 VGATVFEMATGRPPYHS 843
+G ++ M GRPP+ +
Sbjct: 263 LGCVMYTMLLGRPPFET 279
>sp|Q9NYY3|PLK2_HUMAN Serine/threonine-protein kinase PLK2 (Polo-like kinase 1) (PLK-2)
(Serine/threonine-protein kinase SNK) (Serum inducible
kinase)
Length = 685
Score = 71.6 bits (174), Expect = 3e-12
Identities = 42/137 (30%), Positives = 75/137 (54%), Gaps = 2/137 (1%)
Frame = +1
Query: 439 RENFIKQLSTSSSGNYQVVYKTNSMWSEISPPIKYYSQRY*KHLFF--KSSLFDFLI*IG 612
+EN L S + + K + +E P ++YY ++ L + + + + +G
Sbjct: 152 KENIYILLEYCSRRSMAHILKARKVLTE--PEVRYYLRQIVSGLKYLHEQEILHRDLKLG 209
Query: 613 ANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIWS 792
N I + +K+ DFGL+ L+ LE +++T CGT NY+SPEV+N + +G +DIW+
Sbjct: 210 -NFFINEAMELKVGDFGLAARLEP---LEHRRRTICGTPNYLSPEVLNKQGHGCESDIWA 265
Query: 793 VGATVFEMATGRPPYHS 843
+G ++ M GRPP+ +
Sbjct: 266 LGCVMYTMLLGRPPFET 282
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 71.6 bits (174), Expect = 3e-12
Identities = 36/76 (47%), Positives = 48/76 (63%)
Frame = +1
Query: 610 GANILITKQDCIKIVDFGLSRFLQTVSRLEKKKKTFCGTTNYMSPEVINDESYGFPADIW 789
GANIL +K+ DFG S+ LQT+ ++ GT +MSPEVI+ E YG AD+W
Sbjct: 492 GANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVW 551
Query: 790 SVGATVFEMATGRPPY 837
S+G TV EM T +PP+
Sbjct: 552 SLGCTVVEMLTEKPPW 567
Score = 39.7 bits (91), Expect = 0.011
Identities = 20/47 (42%), Positives = 26/47 (55%)
Frame = +2
Query: 437 SEKISLNNYQPLPVETIKLYTKQILCGLKFLHQSNIIHRDIKSIYFL 577
S K L Y L + YT+QIL G+ +LH + I+HRDIK L
Sbjct: 450 SVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANIL 496
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,600,582
Number of Sequences: 369166
Number of extensions: 1555322
Number of successful extensions: 7579
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6625
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)