Planaria EST Database

DrC_00506

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00506
         (912 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O95858|TSN15_HUMAN  Tetraspanin-15 (Tspan-15) (Transmembr...    38   0.045
sp|Q9UKR8|TNS16_HUMAN  Tetraspanin-16 (Tspan-16) (Transmembr...    37   0.10 
sp|P08962|CD63_HUMAN  CD63 antigen (Melanoma-associated anti...    36   0.13 
sp|Q8NG11|TSN14_HUMAN  Tetraspanin-14 (Tspan-14) (Transmembr...    35   0.22 
sp|Q8QZY6|TSN14_MOUSE  Tetraspanin-14 (Tspan-14) (Transmembr...    35   0.38 
sp|P28648|CD63_RAT  CD63 antigen (AD1 antigen)                     34   0.50 
sp|Q9D7W4|TSN17_MOUSE  Tetraspanin-17 (Tspan-17) (Transmembr...    34   0.50 
sp|P62080|TSN5_MOUSE  Tetraspanin-5 (Tspan-5) (Transmembrane...    34   0.65 
sp|Q68VK5|TSN5_RAT  Tetraspanin-5 (Tspan-5) (Transmembrane 4...    34   0.65 
sp|Q96FV3|TSN17_HUMAN  Tetraspanin-17 (Tspan-17) (Transmembr...    33   0.85 

>sp|O95858|TSN15_HUMAN Tetraspanin-15 (Tspan-15) (Transmembrane 4 superfamily member 15)
           (Tetraspan NET-7)
          Length = 294

 Score = 37.7 bits (86), Expect = 0.045
 Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 5/74 (6%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYNKSYG-----IIPFSCCMMVGQTLTPADS 595
           LD   I+D +Q KFKCCG     D+  N  ++ S        +P++CC+   +  T   +
Sbjct: 139 LDFKNIMDFVQKKFKCCGGEDYRDWSKNQYHDCSAPGPLACGVPYTCCI---RNTTEVVN 195

Query: 596 NCTYYPTVNNSYFN 637
               Y T++   F+
Sbjct: 196 TMCGYKTIDKERFS 209

>sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembrane 4 superfamily member 16)
           (Tetraspanin TM4-B)
          Length = 245

 Score = 36.6 bits (83), Expect = 0.10
 Identities = 22/83 (26%), Positives = 34/83 (40%)
 Frame = +2

Query: 434 DVSGIIDLIQAKFKCCGASSSADFVANTGYNKSYGIIPFSCCMMVGQTLTPADSNCTYYP 613
           D S   +L+  K KCCG ++  DF  ++    +    P SCC  +G        +C    
Sbjct: 139 DYSTQWNLVMEKLKCCGVNNYTDFSGSSFEMTTGHTYPRSCCKSIGSV------SCDGRD 192

Query: 614 TVNNSYFNTGCYSQLWNIINSYS 682
              N     GC+ +L  I  + S
Sbjct: 193 VSPNVIHQKGCFHKLLKITKTQS 215

>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
           (Lysosome-associated membrane glycoprotein 3) (LAMP-3)
           (Ocular melanoma-associated antigen) (OMA81H)
           (Granulophysin) (Tetraspanin-30) (Tspan-30)
          Length = 238

 Score = 36.2 bits (82), Expect = 0.13
 Identities = 17/43 (39%), Positives = 27/43 (62%)
 Frame = +2

Query: 440 SGIIDLIQAKFKCCGASSSADFVANTGYNKSYGIIPFSCCMMV 568
           + I+D +QA FKCCGA++  D+      +K+   +P SCC+ V
Sbjct: 133 ASILDRMQADFKCCGAANYTDWEKIPSMSKNR--VPDSCCINV 173

>sp|Q8NG11|TSN14_HUMAN Tetraspanin-14 (Tspan-14) (Transmembrane 4 superfamily member 14)
           (DC-TM4F2)
          Length = 270

 Score = 35.4 bits (80), Expect = 0.22
 Identities = 25/88 (28%), Positives = 35/88 (39%), Gaps = 12/88 (13%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYNKSYGI-------IPFSCCM-----MVGQ 574
           +D+  +ID +Q   +CCGA    D+  N  +N S          +PFSCC+      V  
Sbjct: 138 IDLQNLIDSLQKANQCCGAYGPEDWDLNVYFNCSGASYSREKCGVPFSCCVPDPAQKVVN 197

Query: 575 TLTPADSNCTYYPTVNNSYFNTGCYSQL 658
           T    D         + S F  GC   L
Sbjct: 198 TQCGYDVRIQLKSKWDESIFTKGCIQAL 225

>sp|Q8QZY6|TSN14_MOUSE Tetraspanin-14 (Tspan-14) (Transmembrane 4 superfamily member 14)
          Length = 270

 Score = 34.7 bits (78), Expect = 0.38
 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYNKSYGI-------IPFSCCM 562
           +D+  +ID +Q   +CCGA    D+  N  +N S          +PFSCC+
Sbjct: 138 IDLQNLIDSLQKANQCCGAYGPEDWDLNVYFNCSGASYSREKCGVPFSCCV 188

>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
          Length = 238

 Score = 34.3 bits (77), Expect = 0.50
 Identities = 21/79 (26%), Positives = 38/79 (48%)
 Frame = +2

Query: 332 RNDLSNQGNSTYTTMLINQYVFNKTQRSTGSSGLDVSGIIDLIQAKFKCCGASSSADFVA 511
           R+ + ++ + ++   + N    NKT           + I+D +Q + KCCGAS+  D+  
Sbjct: 108 RDQVKSEFSKSFQKQMQNYLTDNKT-----------ATILDKLQKENKCCGASNYTDWER 156

Query: 512 NTGYNKSYGIIPFSCCMMV 568
             G  K    +P SCC+ +
Sbjct: 157 IPGMAKDR--VPDSCCINI 173

>sp|Q9D7W4|TSN17_MOUSE Tetraspanin-17 (Tspan-17) (Transmembrane 4 superfamily member 17)
           (Tetraspan protein SB134) (F-box only protein 23)
          Length = 270

 Score = 34.3 bits (77), Expect = 0.50
 Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 7/51 (13%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYN-------KSYGIIPFSCCM 562
           LD+  +ID  Q  + CCGA    D+  N  +N       +    +PFSCC+
Sbjct: 140 LDLQNLIDFAQEYWSCCGARGPNDWNLNIYFNCTDLNPSRERCGVPFSCCV 190

>sp|P62080|TSN5_MOUSE Tetraspanin-5 (Tspan-5) (Transmembrane 4 superfamily member 9)
           (Tetraspan NET-4)
 sp|P62079|TSN5_HUMAN Tetraspanin-5 (Tspan-5) (Transmembrane 4 superfamily member 9)
           (Tetraspan NET-4)
          Length = 268

 Score = 33.9 bits (76), Expect = 0.65
 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 7/50 (14%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYN-------KSYGIIPFSCC 559
           +D+  +ID  Q  ++CCGA  + D+  N  +N       +    +PFSCC
Sbjct: 138 IDLQNLIDFTQEYWQCCGAFGADDWNLNIYFNCTDSNASRERCGVPFSCC 187

>sp|Q68VK5|TSN5_RAT Tetraspanin-5 (Tspan-5) (Transmembrane 4 superfamily member 9)
          Length = 268

 Score = 33.9 bits (76), Expect = 0.65
 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 7/50 (14%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYN-------KSYGIIPFSCC 559
           +D+  +ID  Q  ++CCGA  + D+  N  +N       +    +PFSCC
Sbjct: 138 IDLQSLIDFTQEYWQCCGAFGADDWNLNIYFNCTDSNASRERCGVPFSCC 187

>sp|Q96FV3|TSN17_HUMAN Tetraspanin-17 (Tspan-17) (Transmembrane 4 superfamily member 17)
           (Tetraspan protein SB134) (F-box only protein 23)
          Length = 263

 Score = 33.5 bits (75), Expect = 0.85
 Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 7/51 (13%)
 Frame = +2

Query: 431 LDVSGIIDLIQAKFKCCGASSSADFVANTGYN-------KSYGIIPFSCCM 562
           +D+  +ID  Q  + CCGA    D+  N  +N       +    +PFSCC+
Sbjct: 140 IDLQNLIDFAQEYWSCCGARGPNDWNLNIYFNCTDLNPSRERCGVPFSCCV 190

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,884,568
Number of Sequences: 369166
Number of extensions: 1696791
Number of successful extensions: 3773
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3641
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3763
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9270587090
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00506

  1. Dr_sW_013_F14
  2. Dr_sW_012_J02
  3. Dr_sW_027_B10
  4. Dr_sW_017_E05