Planaria EST Database


DrC_00240

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00240
         (885 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q12400|TRM10_YEAST  tRNA (guanine-N(1)-)-methyltransferas...    68   3e-11
sp|Q09287|YQK3_CAEEL  Hypothetical protein C56G2.3 in chromo...    55   2e-07
sp|Q75A17|TRM10_ASHGO  tRNA (guanine-N(1)-)-methyltransferas...    51   5e-06
sp|Q8WXR4|MYO3B_HUMAN  Myosin IIIB                                 32   1.8  
sp|O94813|SLIT2_HUMAN  Slit homolog 2 protein precursor (Sli...    32   3.1  
sp|O94313|CARB_SCHPO  Carbamoyl-phosphate synthase arginine-...    32   3.1  
sp|P46186|RSEB_ECOLI  Sigma-E factor regulatory protein rseB...    30   6.9  
sp|P39993|GEA2_YEAST  ARF guanine-nucleotide exchange factor 2     30   6.9  
sp|Q89AY1|DAPE_BUCBP  Succinyl-diaminopimelate desuccinylase...    30   8.9  
>sp|Q12400|TRM10_YEAST tRNA (guanine-N(1)-)-methyltransferase TRM10 (tRNA
           methyltransferase 10)
          Length = 293

 Score = 68.2 bits (165), Expect = 3e-11
 Identities = 51/190 (26%), Positives = 93/190 (48%), Gaps = 3/190 (1%)
 Frame = +1

Query: 259 KIVFDFSFENDMNRREIKGTVKQVLECYGFNRKLSEPYQLGIVNLQPGSIGFSDLNNMF- 435
           +I+ D SF+  MN +EI     QV   Y  NR+ +   ++ +            L   F 
Sbjct: 96  EIILDCSFDELMNDKEIVSLSNQVTRAYSANRRANHFAEIKVAPFD------KRLKQRFE 149

Query: 436 LSHKSNRFEILENTSFRIDQRPL--TEVYSRKELIYLSPNSSNIFEEGEYDHDATFVIGG 609
            + K+  +E   +  F  D + +   E  S+ +++YL+ ++    E+ E      +++GG
Sbjct: 150 TTLKNTNYENWNHFKFLPDDKIMFGDEHISKDKIVYLTADTEEKLEKLE--PGMRYIVGG 207

Query: 610 LVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGNWR 789
           +VD +    L L KA+K G+  R LP+D +IN ++G + L    V +++ +    + NW+
Sbjct: 208 IVDKNRYKELCLKKAQKMGIPTRRLPIDEYIN-LEGRRVLTTTHVVQLM-LKYFDDHNWK 265

Query: 790 SALRHSLPPR 819
           +A    LPPR
Sbjct: 266 NAFESVLPPR 275
>sp|Q09287|YQK3_CAEEL Hypothetical protein C56G2.3 in chromosome III
          Length = 318

 Score = 55.5 bits (132), Expect = 2e-07
 Identities = 56/278 (20%), Positives = 111/278 (39%), Gaps = 18/278 (6%)
 Frame = +1

Query: 40  LLCLSCHQRVKYLIYLAKIEFAQANRRAKVLKNAAKEKFFIENTNTFF---GKYFN---- 198
           L+C S + ++ Y  YLA  +  + +R       A   K  +E     +   G  +     
Sbjct: 10  LICESINDKIGYWEYLAVTDKRRRSRNKAQSSGAENYKKVLEEQQRIYTAGGMGYGPEMY 69

Query: 199 ----------KKLSDLEIQRAFQSIVLNNQKIVFDFSFENDMNRREIKGTVKQVLECYGF 348
                     K+++++E  + F ++     +I  D  +  +MN+R+      Q+      
Sbjct: 70  QVISNPMRNQKRVNNIEGSKIFAAMNSGAPRIALDIQYVEEMNKRDSGELGNQMQYTISE 129

Query: 349 NRKLSEPYQLGIVNLQPGSIGFSDLNNMFLSHKSNRFEILENTSFRIDQRPLTEVYSRK- 525
           N     P+ L  VN  P         +  + + +  + I +        + + E Y +  
Sbjct: 130 NFSAKSPFVLDFVN-SPSKEFLEQWLSKSVGYYTGNY-INQTILPDFSTKGIKEFYGKSA 187

Query: 526 ELIYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHIN 705
             IY+S N+ ++ + G    D   VIG  V +  +    L+ AR+  ++   LP+ R++ 
Sbjct: 188 NTIYISSNARDVLD-GPLTAD---VIGICVTMGRKRE-ALSAARRANIRAYRLPIHRYVK 242

Query: 706 WVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLPPR 819
           W  G + LP   +  +L       G+W  AL +++  R
Sbjct: 243 WKSGPQYLPFPNIMNVLREVYMNGGDWSRALHNNISKR 280
>sp|Q75A17|TRM10_ASHGO tRNA (guanine-N(1)-)-methyltransferase (tRNA methyltransferase 10)
          Length = 296

 Score = 50.8 bits (120), Expect = 5e-06
 Identities = 46/201 (22%), Positives = 83/201 (41%), Gaps = 5/201 (2%)
 Frame = +1

Query: 259 KIVFDFSFENDMNRREIKGTVKQVLECYGFNRKLSEPYQLGIVNLQPG-----SIGFSDL 423
           K++ D +F+  MN +EI     Q+   Y  N++ +    + + +           G    
Sbjct: 98  KVIIDCAFDELMNEKEIVSLSTQITRAYSANKRENHFADVKVTSFNKRLKERFDCGLKGA 157

Query: 424 NNMFLSHKSNRFEILENTSFRIDQRPLTEVYSRKELIYLSPNSSNIFEEGEYDHDATFVI 603
           N     H    FE  + ++      P T        +YL+ ++    E  E     T+++
Sbjct: 158 NYDAWKH----FEFTDESAL-----PTTNA------VYLTADTDETLETLE--PGTTYIV 200

Query: 604 GGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGN 783
           GG+VD +    L   KA++ G+  R LP+  +I  + G K L    V +I+ +    N +
Sbjct: 201 GGIVDKNRHKALCYNKAKELGIPTRRLPIGEYIK-LCGRKVLTTTHVIQIM-LRYFDNHD 258

Query: 784 WRSALRHSLPPRIVTPIGHRA 846
           W+ A    LP R +  +   A
Sbjct: 259 WKEAFESVLPARKLAELADHA 279
>sp|Q8WXR4|MYO3B_HUMAN Myosin IIIB
          Length = 1341

 Score = 32.3 bits (72), Expect = 1.8
 Identities = 31/128 (24%), Positives = 67/128 (52%), Gaps = 7/128 (5%)
 Frame = +1

Query: 118 RAKVLKNAAKEKFFIENTNTFFGKYFNKKLSDLEI--QRAFQSIVLNNQKIVFDFSFEND 291
           +++V+K AA+EK F      + G +  KKLSD  +  ++  + I     +++ D +   +
Sbjct: 518 KSRVIKQAAREKNFHIFYYIYAGLHHQKKLSDFRLPEEKPPRYIADETGRVMHDIT-SKE 576

Query: 292 MNRREIKGTVKQVLECYGF-NRKLSEPYQL--GIVNLQPGSIGFSDLNNMFLSHKSN--R 456
             RR+ +  ++      GF ++++   Y++  GI+N+  G+I F+ +++   + KS    
Sbjct: 577 SYRRQFE-AIQHCFRIIGFTDKEVHSVYRILAGILNI--GNIEFAAISSQHQTDKSEVPN 633

Query: 457 FEILENTS 480
            E L+N +
Sbjct: 634 AEALQNAA 641
>sp|O94813|SLIT2_HUMAN Slit homolog 2 protein precursor (Slit-2) [Contains: Slit homolog 2
           protein N-product; Slit homolog 2 protein C-product]
          Length = 1529

 Score = 31.6 bits (70), Expect = 3.1
 Identities = 33/139 (23%), Positives = 65/139 (46%), Gaps = 16/139 (11%)
 Frame = +1

Query: 118 RAKVLKNAAKEKFFIENTNTFFGKYFNKKLSDLEIQRAFQ----SIVLNNQKI------V 267
           ++K  + +AKE++FI  T  +  K      +DL      +    ++  +NQK+      +
Sbjct: 472 KSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHI 531

Query: 268 FDFSFENDMNRREIKGTVKQVLECYGFNRKLSEPYQLG-----IVNLQPGSI-GFSDLNN 429
             ++ E  +N  E       VLE  G  +KL +  ++      I +++ G+  G S +N 
Sbjct: 532 PQYTAELRLNNNEF-----TVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNE 586

Query: 430 MFLSHKSNRFEILENTSFR 486
           + L+  SNR E +++  F+
Sbjct: 587 ILLT--SNRLENVQHKMFK 603
>sp|O94313|CARB_SCHPO Carbamoyl-phosphate synthase arginine-specific large chain
           (Arginine-specific carbamoyl-phosphate synthetase,
           ammonia chain)
          Length = 1160

 Score = 31.6 bits (70), Expect = 3.1
 Identities = 40/156 (25%), Positives = 65/156 (41%), Gaps = 10/156 (6%)
 Frame = +1

Query: 301 REIKGTVKQVLECYGFNRKLSEPYQLGIVNLQPGSIG-----FSDLNNMFLSHKSNRF-- 459
           REI  ++K V E     R   E  Q  +  + P  +G     F DL+N        RF  
Sbjct: 447 REIGSSMKSVGEVMAVGRTFEESLQKALRQVDPSFLGFMAMPFKDLDNALSVPTDRRFFA 506

Query: 460 --EILENTSFRIDQ-RPLTEVYSRKELIYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQ 630
             E + N  + ID+   LT++   K  +    N + +++E E        IG L  L+++
Sbjct: 507 VVEAMLNQGYSIDKIHDLTKI--DKWFLSKLANMAKVYKELE-------EIGSLYGLNKE 557

Query: 631 LPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLR 738
           + L   +A+K G        D  I+ + G   L +R
Sbjct: 558 IML---RAKKTGFS------DLQISKLVGASELDVR 584
>sp|P46186|RSEB_ECOLI Sigma-E factor regulatory protein rseB precursor
          Length = 318

 Score = 30.4 bits (67), Expect = 6.9
 Identities = 32/178 (17%), Positives = 70/178 (39%), Gaps = 40/178 (22%)
 Frame = +1

Query: 433 FLSHKSNRFEILENTSFRIDQRPLTEVYS----RKELI-------YLSPNSSNIFEEGEY 579
           F+S      E L     R+D RPL ++      R+E++       Y  P        G+Y
Sbjct: 47  FISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTLNGDY 106

Query: 580 DHDAT----------------FVIGGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWV 711
             D+                 F+  G   ++++L   +    ++G ++       +I W+
Sbjct: 107 IVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYS------YIVWM 160

Query: 712 KGNKSLPLR-----------QVFEILS--IARETNGNWRSALRHSLPPRIVTPIGHRA 846
                LP+R           + F +++  + ++ + + ++  + +LPP +  P+G +A
Sbjct: 161 DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA 218
>sp|P39993|GEA2_YEAST ARF guanine-nucleotide exchange factor 2
          Length = 1459

 Score = 30.4 bits (67), Expect = 6.9
 Identities = 23/73 (31%), Positives = 38/73 (52%), Gaps = 2/73 (2%)
 Frame = +1

Query: 97  EFAQANRRAKVLKNAAKEKFFIENTNTFFGKYFNKKLSDLEIQRAFQSIVLNNQKIVFDF 276
           EF +     ++LK   ++  FIE TN F  K   KK   + I++ F  I  ++ K + +F
Sbjct: 543 EFGRQKNEMEILKKRDRKTEFIECTNAFNEK--PKKGIPMLIEKGF--IASDSDKDIAEF 598

Query: 277 SFEND--MNRREI 309
            F N+  MN++ I
Sbjct: 599 LFNNNNRMNKKTI 611
>sp|Q89AY1|DAPE_BUCBP Succinyl-diaminopimelate desuccinylase (SDAP)
          Length = 377

 Score = 30.0 bits (66), Expect = 8.9
 Identities = 19/67 (28%), Positives = 37/67 (55%)
 Frame = +1

Query: 163 ENTNTFFGKYFNKKLSDLEIQRAFQSIVLNNQKIVFDFSFENDMNRREIKGTVKQVLECY 342
           +N N FF    + ++ D+E + +  ++V +   + F+F F N++   +IK  V+ +L+  
Sbjct: 221 DNGNVFFSPT-SVQIYDIESKSSSDNMVPSELTVKFNFRFSNEITSSDIKKKVELLLK-- 277

Query: 343 GFNRKLS 363
            FN K S
Sbjct: 278 HFNLKYS 284
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,745,306
Number of Sequences: 369166
Number of extensions: 2108581
Number of successful extensions: 6019
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 5747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6016
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8838279920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00240

  1. Dr_sW_006_N11
  2. Dr_sW_025_O07
  3. Dr_sW_020_K20