Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_O07
(436 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q12400|TRM10_YEAST tRNA (guanine-N(1)-)-methyltransferas... 55 8e-08
sp|Q75A17|TRM10_ASHGO tRNA (guanine-N(1)-)-methyltransferas... 44 2e-04
sp|Q09287|YQK3_CAEEL Hypothetical protein C56G2.3 in chromo... 43 3e-04
sp|O70435|PSA3_MOUSE Proteasome subunit alpha type 3 (Prote... 30 2.9
sp|P25788|PSA3_HUMAN Proteasome subunit alpha type 3 (Prote... 30 2.9
sp|P18422|PSA3_RAT Proteasome subunit alpha type 3 (Proteas... 30 2.9
sp|Q7T3T8|ZAR1_BRARE Zygote arrest 1 (Oocyte-specific mater... 30 2.9
sp|P40856|SA185_YEAST SIT4-associating protein SAP185 29 3.8
sp|Q58817|RFCS_METJA Replication factor C small subunit (RF... 29 3.8
sp|P14747|P2B2_YEAST Serine/threonine protein phosphatase 2... 29 5.0
>sp|Q12400|TRM10_YEAST tRNA (guanine-N(1)-)-methyltransferase TRM10 (tRNA
methyltransferase 10)
Length = 293
Score = 54.7 bits (130), Expect = 8e-08
Identities = 32/104 (30%), Positives = 59/104 (56%)
Frame = +2
Query: 59 EVYSRKELIYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQLPLTLAKARKEGLKHRSLP 238
E S+ +++YL+ ++ E+ E +++GG+VD + L L KA+K G+ R LP
Sbjct: 176 EHISKDKIVYLTADTEEKLEKLE--PGMRYIVGGIVDKNRYKELCLKKAQKMGIPTRRLP 233
Query: 239 LDRHINWVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLPPR 370
+D +IN ++G + L V +++ + + NW++A LPPR
Sbjct: 234 IDEYIN-LEGRRVLTTTHVVQLM-LKYFDDHNWKNAFESVLPPR 275
>sp|Q75A17|TRM10_ASHGO tRNA (guanine-N(1)-)-methyltransferase (tRNA methyltransferase 10)
Length = 296
Score = 43.5 bits (101), Expect = 2e-04
Identities = 29/105 (27%), Positives = 51/105 (48%)
Frame = +2
Query: 83 IYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWV 262
+YL+ ++ E E T+++GG+VD + L KA++ G+ R LP+ +I +
Sbjct: 179 VYLTADTDETLETLE--PGTTYIVGGIVDKNRHKALCYNKAKELGIPTRRLPIGEYIK-L 235
Query: 263 KGNKSLPLRQVFEILSIARETNGNWRSALRHSLPPRIVTPIGHRA 397
G K L V +I+ + N +W+ A LP R + + A
Sbjct: 236 CGRKVLTTTHVIQIM-LRYFDNHDWKEAFESVLPARKLAELADHA 279
>sp|Q09287|YQK3_CAEEL Hypothetical protein C56G2.3 in chromosome III
Length = 318
Score = 42.7 bits (99), Expect = 3e-04
Identities = 28/107 (26%), Positives = 51/107 (47%), Gaps = 1/107 (0%)
Frame = +2
Query: 53 LTEVYSRK-ELIYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQLPLTLAKARKEGLKHR 229
+ E Y + IY+S N+ ++ + G D VIG V + + L+ AR+ ++
Sbjct: 179 IKEFYGKSANTIYISSNARDVLD-GPLTAD---VIGICVTMGRKRE-ALSAARRANIRAY 233
Query: 230 SLPLDRHINWVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLPPR 370
LP+ R++ W G + LP + +L G+W AL +++ R
Sbjct: 234 RLPIHRYVKWKSGPQYLPFPNIMNVLREVYMNGGDWSRALHNNISKR 280
>sp|O70435|PSA3_MOUSE Proteasome subunit alpha type 3 (Proteasome component C8)
(Macropain subunit C8) (Multicatalytic endopeptidase
complex subunit C8) (Proteasome subunit K)
Length = 255
Score = 29.6 bits (65), Expect = 2.9
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = +2
Query: 188 LTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLP 364
L L+K +EG R +DRH+ L + IARE N+RS +++P
Sbjct: 53 LVLSKLYEEGSNKRLFNVDRHVGMAVAGL---LADARSLADIAREEASNFRSNFGYNIP 108
>sp|P25788|PSA3_HUMAN Proteasome subunit alpha type 3 (Proteasome component C8)
(Macropain subunit C8) (Multicatalytic endopeptidase
complex subunit C8)
Length = 255
Score = 29.6 bits (65), Expect = 2.9
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = +2
Query: 188 LTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLP 364
L L+K +EG R +DRH+ L + IARE N+RS +++P
Sbjct: 53 LVLSKLYEEGSNKRLFNVDRHVGMAVAGL---LADARSLADIAREEASNFRSNFGYNIP 108
>sp|P18422|PSA3_RAT Proteasome subunit alpha type 3 (Proteasome component C8)
(Macropain subunit C8) (Multicatalytic endopeptidase
complex subunit C8) (Proteasome subunit K)
Length = 255
Score = 29.6 bits (65), Expect = 2.9
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = +2
Query: 188 LTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLP 364
L L+K +EG R +DRH+ L + IARE N+RS +++P
Sbjct: 53 LVLSKLYEEGSNKRLFNVDRHVGMAVAGL---LADARSLADIAREEASNFRSNFGYNIP 108
>sp|Q7T3T8|ZAR1_BRARE Zygote arrest 1 (Oocyte-specific maternal effect factor)
Length = 329
Score = 29.6 bits (65), Expect = 2.9
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 6/70 (8%)
Frame = +2
Query: 65 YSRKELIYLSPNSSNIF----EEGEYDHDATFVIGGLVDLSEQLPLTLAKARKEGLKHRS 232
+ R + +Y S + EEGE + D + VD +E+L RK+G K
Sbjct: 127 FPRTQAVYSPVESRRLVSLFREEGEEEEDTDLEVTETVDSAEKLESAEKNVRKQGKKSAK 186
Query: 233 LPL--DRHIN 256
PL +++IN
Sbjct: 187 QPLSPEKNIN 196
>sp|P40856|SA185_YEAST SIT4-associating protein SAP185
Length = 1058
Score = 29.3 bits (64), Expect = 3.8
Identities = 18/80 (22%), Positives = 37/80 (46%)
Frame = +2
Query: 131 DHDATFVIGGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILS 310
D DAT + G V+ ++PL L ++ + K ++P N ++ ++ +
Sbjct: 882 DSDATEQVPGEVNRDHKIPLKLKRSFTDACKSETIP----------NNTVNAKEE-SVFQ 930
Query: 311 IARETNGNWRSALRHSLPPR 370
+ E + W S+ +S+P R
Sbjct: 931 FSNELSDGWESSPSNSIPKR 950
>sp|Q58817|RFCS_METJA Replication factor C small subunit (RFC small subunit) (Clamp loader
small subunit) [Contains: Mja RFC-1 intein; Mja RFC-2
intein; Mja RFC-3 intein]
Length = 1847
Score = 29.3 bits (64), Expect = 3.8
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 251 INWVKGNKSLPLRQVFEILS-IARETNGNWRSALRHSL 361
+ W K N LP + +++ + + NGNWR LRH L
Sbjct: 944 MKWKKSNL-LPAEPIIKMIKKLENKINGNWRYILRHQL 980
>sp|P14747|P2B2_YEAST Serine/threonine protein phosphatase 2B catalytic subunit A2
(Calcineurin A2) (Calmodulin-binding protein 2)
Length = 604
Score = 28.9 bits (63), Expect = 5.0
Identities = 26/78 (33%), Positives = 35/78 (44%), Gaps = 6/78 (7%)
Frame = +2
Query: 107 NIFEEGEYDHDATFVIGGLVDLSEQLP------LTLAKARKEGLKHRSLPLDRHINWVKG 268
NI E E ++D T VI LV ++LP T A KH S+ D H
Sbjct: 448 NICTEDELEND-TPVIEELVGTDKKLPQAGKSEATPQPATSASPKHASILDDEHRRKALR 506
Query: 269 NKSLPLRQVFEILSIARE 322
NK L + +V + S+ RE
Sbjct: 507 NKILAVAKVSRMYSVLRE 524
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,370,226
Number of Sequences: 369166
Number of extensions: 1011406
Number of successful extensions: 3159
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3050
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3158
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2194785120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)