Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_N11
(885 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q12400|TRM10_YEAST tRNA (guanine-N(1)-)-methyltransferas... 68 3e-11
sp|Q09287|YQK3_CAEEL Hypothetical protein C56G2.3 in chromo... 55 2e-07
sp|Q75A17|TRM10_ASHGO tRNA (guanine-N(1)-)-methyltransferas... 51 5e-06
sp|Q8WXR4|MYO3B_HUMAN Myosin IIIB 32 1.8
sp|O94813|SLIT2_HUMAN Slit homolog 2 protein precursor (Sli... 32 3.1
sp|O94313|CARB_SCHPO Carbamoyl-phosphate synthase arginine-... 32 3.1
sp|P46186|RSEB_ECOLI Sigma-E factor regulatory protein rseB... 30 6.9
sp|P39993|GEA2_YEAST ARF guanine-nucleotide exchange factor 2 30 6.9
sp|Q89AY1|DAPE_BUCBP Succinyl-diaminopimelate desuccinylase... 30 8.9
>sp|Q12400|TRM10_YEAST tRNA (guanine-N(1)-)-methyltransferase TRM10 (tRNA
methyltransferase 10)
Length = 293
Score = 68.2 bits (165), Expect = 3e-11
Identities = 51/190 (26%), Positives = 93/190 (48%), Gaps = 3/190 (1%)
Frame = +1
Query: 259 KIVFDFSFENDMNRREIKGTVKQVLECYGFNRKLSEPYQLGIVNLQPGSIGFSDLNNMF- 435
+I+ D SF+ MN +EI QV Y NR+ + ++ + L F
Sbjct: 96 EIILDCSFDELMNDKEIVSLSNQVTRAYSANRRANHFAEIKVAPFD------KRLKQRFE 149
Query: 436 LSHKSNRFEILENTSFRIDQRPL--TEVYSRKELIYLSPNSSNIFEEGEYDHDATFVIGG 609
+ K+ +E + F D + + E S+ +++YL+ ++ E+ E +++GG
Sbjct: 150 TTLKNTNYENWNHFKFLPDDKIMFGDEHISKDKIVYLTADTEEKLEKLE--PGMRYIVGG 207
Query: 610 LVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGNWR 789
+VD + L L KA+K G+ R LP+D +IN ++G + L V +++ + + NW+
Sbjct: 208 IVDKNRYKELCLKKAQKMGIPTRRLPIDEYIN-LEGRRVLTTTHVVQLM-LKYFDDHNWK 265
Query: 790 SALRHSLPPR 819
+A LPPR
Sbjct: 266 NAFESVLPPR 275
>sp|Q09287|YQK3_CAEEL Hypothetical protein C56G2.3 in chromosome III
Length = 318
Score = 55.5 bits (132), Expect = 2e-07
Identities = 56/278 (20%), Positives = 111/278 (39%), Gaps = 18/278 (6%)
Frame = +1
Query: 40 LLCLSCHQRVKYLIYLAKIEFAQANRRAKVLKNAAKEKFFIENTNTFF---GKYFN---- 198
L+C S + ++ Y YLA + + +R A K +E + G +
Sbjct: 10 LICESINDKIGYWEYLAVTDKRRRSRNKAQSSGAENYKKVLEEQQRIYTAGGMGYGPEMY 69
Query: 199 ----------KKLSDLEIQRAFQSIVLNNQKIVFDFSFENDMNRREIKGTVKQVLECYGF 348
K+++++E + F ++ +I D + +MN+R+ Q+
Sbjct: 70 QVISNPMRNQKRVNNIEGSKIFAAMNSGAPRIALDIQYVEEMNKRDSGELGNQMQYTISE 129
Query: 349 NRKLSEPYQLGIVNLQPGSIGFSDLNNMFLSHKSNRFEILENTSFRIDQRPLTEVYSRK- 525
N P+ L VN P + + + + + I + + + E Y +
Sbjct: 130 NFSAKSPFVLDFVN-SPSKEFLEQWLSKSVGYYTGNY-INQTILPDFSTKGIKEFYGKSA 187
Query: 526 ELIYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHIN 705
IY+S N+ ++ + G D VIG V + + L+ AR+ ++ LP+ R++
Sbjct: 188 NTIYISSNARDVLD-GPLTAD---VIGICVTMGRKRE-ALSAARRANIRAYRLPIHRYVK 242
Query: 706 WVKGNKSLPLRQVFEILSIARETNGNWRSALRHSLPPR 819
W G + LP + +L G+W AL +++ R
Sbjct: 243 WKSGPQYLPFPNIMNVLREVYMNGGDWSRALHNNISKR 280
>sp|Q75A17|TRM10_ASHGO tRNA (guanine-N(1)-)-methyltransferase (tRNA methyltransferase 10)
Length = 296
Score = 50.8 bits (120), Expect = 5e-06
Identities = 46/201 (22%), Positives = 83/201 (41%), Gaps = 5/201 (2%)
Frame = +1
Query: 259 KIVFDFSFENDMNRREIKGTVKQVLECYGFNRKLSEPYQLGIVNLQPG-----SIGFSDL 423
K++ D +F+ MN +EI Q+ Y N++ + + + + G
Sbjct: 98 KVIIDCAFDELMNEKEIVSLSTQITRAYSANKRENHFADVKVTSFNKRLKERFDCGLKGA 157
Query: 424 NNMFLSHKSNRFEILENTSFRIDQRPLTEVYSRKELIYLSPNSSNIFEEGEYDHDATFVI 603
N H FE + ++ P T +YL+ ++ E E T+++
Sbjct: 158 NYDAWKH----FEFTDESAL-----PTTNA------VYLTADTDETLETLE--PGTTYIV 200
Query: 604 GGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLRQVFEILSIARETNGN 783
GG+VD + L KA++ G+ R LP+ +I + G K L V +I+ + N +
Sbjct: 201 GGIVDKNRHKALCYNKAKELGIPTRRLPIGEYIK-LCGRKVLTTTHVIQIM-LRYFDNHD 258
Query: 784 WRSALRHSLPPRIVTPIGHRA 846
W+ A LP R + + A
Sbjct: 259 WKEAFESVLPARKLAELADHA 279
>sp|Q8WXR4|MYO3B_HUMAN Myosin IIIB
Length = 1341
Score = 32.3 bits (72), Expect = 1.8
Identities = 31/128 (24%), Positives = 67/128 (52%), Gaps = 7/128 (5%)
Frame = +1
Query: 118 RAKVLKNAAKEKFFIENTNTFFGKYFNKKLSDLEI--QRAFQSIVLNNQKIVFDFSFEND 291
+++V+K AA+EK F + G + KKLSD + ++ + I +++ D + +
Sbjct: 518 KSRVIKQAAREKNFHIFYYIYAGLHHQKKLSDFRLPEEKPPRYIADETGRVMHDIT-SKE 576
Query: 292 MNRREIKGTVKQVLECYGF-NRKLSEPYQL--GIVNLQPGSIGFSDLNNMFLSHKSN--R 456
RR+ + ++ GF ++++ Y++ GI+N+ G+I F+ +++ + KS
Sbjct: 577 SYRRQFE-AIQHCFRIIGFTDKEVHSVYRILAGILNI--GNIEFAAISSQHQTDKSEVPN 633
Query: 457 FEILENTS 480
E L+N +
Sbjct: 634 AEALQNAA 641
>sp|O94813|SLIT2_HUMAN Slit homolog 2 protein precursor (Slit-2) [Contains: Slit homolog 2
protein N-product; Slit homolog 2 protein C-product]
Length = 1529
Score = 31.6 bits (70), Expect = 3.1
Identities = 33/139 (23%), Positives = 65/139 (46%), Gaps = 16/139 (11%)
Frame = +1
Query: 118 RAKVLKNAAKEKFFIENTNTFFGKYFNKKLSDLEIQRAFQ----SIVLNNQKI------V 267
++K + +AKE++FI T + K +DL + ++ +NQK+ +
Sbjct: 472 KSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHI 531
Query: 268 FDFSFENDMNRREIKGTVKQVLECYGFNRKLSEPYQLG-----IVNLQPGSI-GFSDLNN 429
++ E +N E VLE G +KL + ++ I +++ G+ G S +N
Sbjct: 532 PQYTAELRLNNNEF-----TVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNE 586
Query: 430 MFLSHKSNRFEILENTSFR 486
+ L+ SNR E +++ F+
Sbjct: 587 ILLT--SNRLENVQHKMFK 603
>sp|O94313|CARB_SCHPO Carbamoyl-phosphate synthase arginine-specific large chain
(Arginine-specific carbamoyl-phosphate synthetase,
ammonia chain)
Length = 1160
Score = 31.6 bits (70), Expect = 3.1
Identities = 40/156 (25%), Positives = 65/156 (41%), Gaps = 10/156 (6%)
Frame = +1
Query: 301 REIKGTVKQVLECYGFNRKLSEPYQLGIVNLQPGSIG-----FSDLNNMFLSHKSNRF-- 459
REI ++K V E R E Q + + P +G F DL+N RF
Sbjct: 447 REIGSSMKSVGEVMAVGRTFEESLQKALRQVDPSFLGFMAMPFKDLDNALSVPTDRRFFA 506
Query: 460 --EILENTSFRIDQ-RPLTEVYSRKELIYLSPNSSNIFEEGEYDHDATFVIGGLVDLSEQ 630
E + N + ID+ LT++ K + N + +++E E IG L L+++
Sbjct: 507 VVEAMLNQGYSIDKIHDLTKI--DKWFLSKLANMAKVYKELE-------EIGSLYGLNKE 557
Query: 631 LPLTLAKARKEGLKHRSLPLDRHINWVKGNKSLPLR 738
+ L +A+K G D I+ + G L +R
Sbjct: 558 IML---RAKKTGFS------DLQISKLVGASELDVR 584
>sp|P46186|RSEB_ECOLI Sigma-E factor regulatory protein rseB precursor
Length = 318
Score = 30.4 bits (67), Expect = 6.9
Identities = 32/178 (17%), Positives = 70/178 (39%), Gaps = 40/178 (22%)
Frame = +1
Query: 433 FLSHKSNRFEILENTSFRIDQRPLTEVYS----RKELI-------YLSPNSSNIFEEGEY 579
F+S E L R+D RPL ++ R+E++ Y P G+Y
Sbjct: 47 FISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTLNGDY 106
Query: 580 DHDAT----------------FVIGGLVDLSEQLPLTLAKARKEGLKHRSLPLDRHINWV 711
D+ F+ G ++++L + ++G ++ +I W+
Sbjct: 107 IVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYS------YIVWM 160
Query: 712 KGNKSLPLR-----------QVFEILS--IARETNGNWRSALRHSLPPRIVTPIGHRA 846
LP+R + F +++ + ++ + + ++ + +LPP + P+G +A
Sbjct: 161 DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA 218
>sp|P39993|GEA2_YEAST ARF guanine-nucleotide exchange factor 2
Length = 1459
Score = 30.4 bits (67), Expect = 6.9
Identities = 23/73 (31%), Positives = 38/73 (52%), Gaps = 2/73 (2%)
Frame = +1
Query: 97 EFAQANRRAKVLKNAAKEKFFIENTNTFFGKYFNKKLSDLEIQRAFQSIVLNNQKIVFDF 276
EF + ++LK ++ FIE TN F K KK + I++ F I ++ K + +F
Sbjct: 543 EFGRQKNEMEILKKRDRKTEFIECTNAFNEK--PKKGIPMLIEKGF--IASDSDKDIAEF 598
Query: 277 SFEND--MNRREI 309
F N+ MN++ I
Sbjct: 599 LFNNNNRMNKKTI 611
>sp|Q89AY1|DAPE_BUCBP Succinyl-diaminopimelate desuccinylase (SDAP)
Length = 377
Score = 30.0 bits (66), Expect = 8.9
Identities = 19/67 (28%), Positives = 37/67 (55%)
Frame = +1
Query: 163 ENTNTFFGKYFNKKLSDLEIQRAFQSIVLNNQKIVFDFSFENDMNRREIKGTVKQVLECY 342
+N N FF + ++ D+E + + ++V + + F+F F N++ +IK V+ +L+
Sbjct: 221 DNGNVFFSPT-SVQIYDIESKSSSDNMVPSELTVKFNFRFSNEITSSDIKKKVELLLK-- 277
Query: 343 GFNRKLS 363
FN K S
Sbjct: 278 HFNLKYS 284
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,745,306
Number of Sequences: 369166
Number of extensions: 2108581
Number of successful extensions: 6019
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 5747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6016
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8838279920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)