Planaria EST Database

DrC_00219

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00219
         (874 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9UNH7|SNX6_HUMAN  Sorting nexin-6 (TRAF4-associated fact...   158   2e-38
sp|Q5R613|SNX6_PONPY  Sorting nexin-6                             158   2e-38
sp|Q9Y5X3|SNX5_HUMAN  Sorting nexin-5                             142   2e-33
sp|Q9D8U8|SNX5_MOUSE  Sorting nexin-5                             141   3e-33
sp|P25343|RV161_YEAST  Reduced viability upon starvation pro...    37   0.072
sp|P30199|EPIP_STAEP  Epidermin leader peptide-processing se...    35   0.21 
sp|Q8NDV3|SM1L2_HUMAN  Structural maintenance of chromosome ...    35   0.21 
sp|P50478|AMPH_CHICK  Amphiphysin                                  33   0.79 
sp|Q92JK8|CLPB_RICCN  Chaperone clpB                               32   1.8  
sp|P49418|AMPH_HUMAN  Amphiphysin                                  32   3.0  

>sp|Q9UNH7|SNX6_HUMAN Sorting nexin-6 (TRAF4-associated factor 2)
          Length = 406

 Score =  158 bits (399), Expect = 2e-38
 Identities = 76/136 (55%), Positives = 108/136 (79%)
 Frame = +2

Query: 239 FLALSKVFEEVRKLENRIATDEDLKLSDTLRYYQNDSQAAKALLYRRSRALADYDSASKA 418
           FL +S++F++ RK+E R++ DEDLKLSD L+YY  +SQAAK LLYRRSR+L DY++A+KA
Sbjct: 267 FLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKA 326

Query: 419 VEKARIKGKEVAAAEGTQSISYDKFKKISESAKTQIMDFKQRRIAYFKKNLTELTKLQLK 598
           ++KAR K K+V  AE +Q +   KF+KISESAK +++DFK RR+A F+KNL EL +L+LK
Sbjct: 327 LDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELK 386

Query: 599 HARSHIQVLRTALNAL 646
           HA+ ++Q+L+  L  L
Sbjct: 387 HAKGNLQLLQNCLAVL 402


 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 18/44 (40%), Positives = 33/44 (75%)
 Frame = +1

Query: 58  FFLNEKVYLVDYYLAVREATMKADKMTRQHKTMSDGYIAVSTSL 189
           FF +E+ +L++Y+  V++A+ K+D+MTR HK+ +D Y  + +SL
Sbjct: 209 FFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSL 252

>sp|Q5R613|SNX6_PONPY Sorting nexin-6
          Length = 406

 Score =  158 bits (399), Expect = 2e-38
 Identities = 76/136 (55%), Positives = 108/136 (79%)
 Frame = +2

Query: 239 FLALSKVFEEVRKLENRIATDEDLKLSDTLRYYQNDSQAAKALLYRRSRALADYDSASKA 418
           FL +S++F++ RK+E R++ DEDLKLSD L+YY  +SQAAK LLYRRSR+L DY++A+KA
Sbjct: 267 FLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKA 326

Query: 419 VEKARIKGKEVAAAEGTQSISYDKFKKISESAKTQIMDFKQRRIAYFKKNLTELTKLQLK 598
           ++KAR K K+V  AE +Q +   KF+KISESAK +++DFK RR+A F+KNL EL +L+LK
Sbjct: 327 LDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELK 386

Query: 599 HARSHIQVLRTALNAL 646
           HA+ ++Q+L+  L  L
Sbjct: 387 HAKGNLQLLQNCLAVL 402


 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 18/44 (40%), Positives = 33/44 (75%)
 Frame = +1

Query: 58  FFLNEKVYLVDYYLAVREATMKADKMTRQHKTMSDGYIAVSTSL 189
           FF +E+ +L++Y+  V++A+ K+D+MTR HK+ +D Y  + +SL
Sbjct: 209 FFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSL 252

>sp|Q9Y5X3|SNX5_HUMAN Sorting nexin-5
          Length = 404

 Score =  142 bits (357), Expect = 2e-33
 Identities = 63/136 (46%), Positives = 111/136 (81%)
 Frame = +2

Query: 242 LALSKVFEEVRKLENRIATDEDLKLSDTLRYYQNDSQAAKALLYRRSRALADYDSASKAV 421
           L ++++FE++RK+E R+++DEDLKL++ LRYY  + +AAK LLYRR++AL DY++++KA+
Sbjct: 267 LKVAELFEKLRKVEGRVSSDEDLKLTELLRYYMLNIEAAKDLLYRRTKALIDYENSNKAL 326

Query: 422 EKARIKGKEVAAAEGTQSISYDKFKKISESAKTQIMDFKQRRIAYFKKNLTELTKLQLKH 601
           +KAR+K K+V  AE  Q     KF+++SESAK ++++FK++R+A F+KNL E+++L++KH
Sbjct: 327 DKARLKSKDVKLAEAHQQECCQKFEQLSESAKEELINFKRKRVAAFRKNLIEMSELEIKH 386

Query: 602 ARSHIQVLRTALNALK 649
           AR+++ +L++ ++  K
Sbjct: 387 ARNNVSLLQSCIDLFK 402


 Score = 48.5 bits (114), Expect = 2e-05
 Identities = 21/49 (42%), Positives = 33/49 (67%)
 Frame = +1

Query: 43  KKKYSFFLNEKVYLVDYYLAVREATMKADKMTRQHKTMSDGYIAVSTSL 189
           K+   FF  EK +L++YY  ++++ +KADKMTR HK ++D YI  +  L
Sbjct: 203 KEVDDFFEQEKNFLINYYNRIKDSCVKADKMTRSHKNVADDYIHTAACL 251

>sp|Q9D8U8|SNX5_MOUSE Sorting nexin-5
          Length = 404

 Score =  141 bits (355), Expect = 3e-33
 Identities = 63/136 (46%), Positives = 111/136 (81%)
 Frame = +2

Query: 242 LALSKVFEEVRKLENRIATDEDLKLSDTLRYYQNDSQAAKALLYRRSRALADYDSASKAV 421
           L ++++FE++RK+E R+++DEDLKL++ LRYY  + +AAK LLYRR++AL DY++++KA+
Sbjct: 267 LKVAELFEKLRKVEGRVSSDEDLKLTELLRYYMLNIEAAKDLLYRRTKALIDYENSNKAL 326

Query: 422 EKARIKGKEVAAAEGTQSISYDKFKKISESAKTQIMDFKQRRIAYFKKNLTELTKLQLKH 601
           +KAR+K K+V  AE  Q     KF+++SESAK ++++FK++R+A F+KNL E+++L++KH
Sbjct: 327 DKARLKSKDVKLAETHQQECCQKFEQLSESAKEELINFKRKRVAAFRKNLIEMSELEIKH 386

Query: 602 ARSHIQVLRTALNALK 649
           AR+++ +L++ ++  K
Sbjct: 387 ARNNVSLLQSCIDLFK 402


 Score = 47.8 bits (112), Expect = 4e-05
 Identities = 21/49 (42%), Positives = 32/49 (65%)
 Frame = +1

Query: 43  KKKYSFFLNEKVYLVDYYLAVREATMKADKMTRQHKTMSDGYIAVSTSL 189
           K+   FF  EK +L++YY  ++++  KADKMTR HK ++D YI  +  L
Sbjct: 203 KEVDDFFEQEKNFLINYYNRIKDSCAKADKMTRSHKNVADDYIHTAACL 251

>sp|P25343|RV161_YEAST Reduced viability upon starvation protein 161
          Length = 265

 Score = 37.0 bits (84), Expect = 0.072
 Identities = 23/93 (24%), Positives = 44/93 (47%), Gaps = 4/93 (4%)
 Frame = +2

Query: 332 YYQNDSQAAKALLYRRSRALADYDSAS----KAVEKARIKGKEVAAAEGTQSISYDKFKK 499
           Y++   +A K    +R     D+D+A     + V+K      ++  AE   S++ D F+ 
Sbjct: 126 YFKEIEEAIK----KRDHKKQDFDAAKAKVRRLVDKPAKDASKLPRAEKELSLAKDIFEN 181

Query: 500 ISESAKTQIMDFKQRRIAYFKKNLTELTKLQLK 598
           ++   KT++      R+ YF  +   L K+QL+
Sbjct: 182 LNNQLKTELPQLVSLRVPYFDPSFEALIKIQLR 214

>sp|P30199|EPIP_STAEP Epidermin leader peptide-processing serine protease epiP precursor
          Length = 461

 Score = 35.4 bits (80), Expect = 0.21
 Identities = 22/59 (37%), Positives = 30/59 (50%)
 Frame = +2

Query: 332 YYQNDSQAAKALLYRRSRALADYDSASKAVEKARIKGKEVAAAEGTQSISYDKFKKISE 508
           Y   D+Q  K L   R     DYD+  KA+  A+ KG  V AA G   I+  K K+I++
Sbjct: 255 YLIKDNQNKKKL---RDDEKVDYDALQKAINYAQKKGSIVVAAVGNDGINVKKVKEINK 310

>sp|Q8NDV3|SM1L2_HUMAN Structural maintenance of chromosome 1-like 2 protein (SMC1beta
            protein)
          Length = 1235

 Score = 35.4 bits (80), Expect = 0.21
 Identities = 32/128 (25%), Positives = 59/128 (46%), Gaps = 11/128 (8%)
 Frame = +2

Query: 302  EDLKLSDTLRYYQNDSQAAKALLYRRSRALADYDSASKAVEKARIKGKEVAAAEGTQSIS 481
            E +K    + +YQ  SQ    LL   S+ +      S+ +++ + + KE    E    + 
Sbjct: 720  EMIKKKHLVAFYQEQSQLQSELLNIESQCIM----LSEGIKERQRRIKEFQ--EKIDKVE 773

Query: 482  YDKFKKISESAKTQ-IMDFKQRRIA----------YFKKNLTELTKLQLKHARSHIQVLR 628
             D F+   E    + I +F+ + +           ++KK LT L  +QL+++RSH++   
Sbjct: 774  DDIFQHFCEEIGVENIREFENKHVKRQQEIDQKRYFYKKMLTRLN-VQLEYSRSHLKKKL 832

Query: 629  TALNALKE 652
              +N LKE
Sbjct: 833  NKINTLKE 840

>sp|P50478|AMPH_CHICK Amphiphysin
          Length = 682

 Score = 33.5 bits (75), Expect = 0.79
 Identities = 24/97 (24%), Positives = 43/97 (44%), Gaps = 5/97 (5%)
 Frame = +2

Query: 323 TLRYYQNDSQAAKALLYRRSRALADYDSASKAVE-----KARIKGKEVAAAEGTQSISYD 487
           TL  Y       K  + +RSR L DYDSA   +E     K + +G+   A E  Q  +  
Sbjct: 120 TLDTYLGQFPDIKTRIAKRSRKLVDYDSARHHLEALQSSKRKDEGRITKAEEEFQK-AQK 178

Query: 488 KFKKISESAKTQIMDFKQRRIAYFKKNLTELTKLQLK 598
            F++ +   + ++     RR+ ++      ++ L+ K
Sbjct: 179 VFEEFNTDLQEELPSLWSRRVGFYVNTFKNVSSLEAK 215

>sp|Q92JK8|CLPB_RICCN Chaperone clpB
          Length = 857

 Score = 32.3 bits (72), Expect = 1.8
 Identities = 20/54 (37%), Positives = 28/54 (51%)
 Frame = +2

Query: 497 KISESAKTQIMDFKQRRIAYFKKNLTELTKLQLKHARSHIQVLRTALNALKEPS 658
           KI  S+K + +D   RRI   K  L  L K   +H++  I+ L TAL  L+  S
Sbjct: 403 KIELSSKPEELDELDRRIIQIKIELAALKKENDEHSKKKIEHLTTALEKLESQS 456

>sp|P49418|AMPH_HUMAN Amphiphysin
          Length = 695

 Score = 31.6 bits (70), Expect = 3.0
 Identities = 22/96 (22%), Positives = 45/96 (46%), Gaps = 4/96 (4%)
 Frame = +2

Query: 323 TLRYYQNDSQAAKALLYRRSRALADYDSAS---KAVEKARIKGK-EVAAAEGTQSISYDK 490
           TL  Y       K  + +RSR L DYDSA    +A++ ++ K +  ++ AE     +   
Sbjct: 120 TLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKV 179

Query: 491 FKKISESAKTQIMDFKQRRIAYFKKNLTELTKLQLK 598
           F++ +   + ++     RR+ ++      ++ L+ K
Sbjct: 180 FEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAK 215

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,579,169
Number of Sequences: 369166
Number of extensions: 1466878
Number of successful extensions: 4289
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4287
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00219

  1. Dr_sW_008_I10
  2. Dr_sW_024_G15
  3. Dr_sW_006_H10