Planaria EST Database

DrC_00214

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00214
         (1370 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P97329|KI20A_MOUSE  Kinesin family member 20A (Rabkinesin...    39   0.035
sp|O95235|KI20A_HUMAN  Kinesin family member 20A (Rabkinesin...    37   0.10 
sp|Q06106|MRD1_YEAST  Multiple RNA-binding domain-containing...    37   0.13 
sp|Q9JMH9|MY18A_MOUSE  Myosin-18A (Myosin XVIIIa) (Myosin co...    34   0.87 
sp|P08799|MYS2_DICDI  Myosin II heavy chain, non muscle            34   0.87 
sp|P25386|USO1_YEAST  Intracellular protein transport protei...    34   1.1  
sp|O67825|IF2_AQUAE  Translation initiation factor IF-2            34   1.1  
sp|Q07847|VE1_HPV63  Replication protein E1                        33   1.5  
sp|P02566|MYO4_CAEEL  Myosin-4 (Myosin heavy chain B) (MHC B...    32   3.3  
sp|P36353|VNS5_MSTV  Nonstructural protein NS5                     32   3.3  

>sp|P97329|KI20A_MOUSE Kinesin family member 20A (Rabkinesin-6) (Rab6-interacting
           kinesin-like protein) (Kinesin-like protein 174)
          Length = 887

 Score = 38.9 bits (89), Expect = 0.035
 Identities = 26/107 (24%), Positives = 48/107 (44%), Gaps = 3/107 (2%)
 Frame = +1

Query: 301 QEIEEFDDEYVPLPDNLPERSKE---EMETLLWTQKRDSQIREWQEVKQLEREKVKLEHQ 471
           ++I+E D++   L   L E  ++   +    L   +R  ++      +Q +  K +LE  
Sbjct: 638 EQIQERDEKIEELETLLQEAKQQPAAQQSGGLSLLRRSQRLAASASTQQFQEVKAELEQC 697

Query: 472 VRSMQRRNSRAHKTSDILKVPPFAKPHHLAIDQDSEDKRFQFPLLTT 612
              +    +  HK   +LK PP AKP  + +D+  E+ +    LL T
Sbjct: 698 KTELSSTTAELHKYQQVLKPPPPAKPFTIDVDKKLEEGQKNIRLLRT 744

>sp|O95235|KI20A_HUMAN Kinesin family member 20A (Rabkinesin-6) (Rab6-interacting
           kinesin-like protein) (GG10_2)
          Length = 890

 Score = 37.4 bits (85), Expect = 0.10
 Identities = 28/111 (25%), Positives = 49/111 (44%), Gaps = 7/111 (6%)
 Frame = +1

Query: 301 QEIEEFDDEYVPLPDNLPERSKEEM-------ETLLWTQKRDSQIREWQEVKQLEREKVK 459
           +EI+E D++   L   L E  ++ +       E  L   +R  ++      +QL+  K K
Sbjct: 639 EEIQERDEKIEELEALLQEARQQSVAHQQSGSELAL---RRSQRLAASASTQQLQEVKAK 695

Query: 460 LEHQVRSMQRRNSRAHKTSDILKVPPFAKPHHLAIDQDSEDKRFQFPLLTT 612
           L+     +       HK   +L+ PP AKP  + +D+  E+ +    LL T
Sbjct: 696 LQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRT 746

>sp|Q06106|MRD1_YEAST Multiple RNA-binding domain-containing protein 1
          Length = 887

 Score = 37.0 bits (84), Expect = 0.13
 Identities = 25/92 (27%), Positives = 48/92 (52%), Gaps = 3/92 (3%)
 Frame = +1

Query: 358 RSKEEMETLLWTQKRDSQIREWQEV---KQLEREKVKLEHQVRSMQRRNSRAHKTSDILK 528
           R  ++++  + T K  SQ+  W++V   K +E EK+K E +  S+Q  +  AH  + + +
Sbjct: 146 RKNKQLQEFMETMKPSSQVTSWEKVGIDKSIEDEKLKREEEDSSVQGNSLLAHALA-LKE 204

Query: 529 VPPFAKPHHLAIDQDSEDKRFQFPLLTTNDDE 624
                +  +L I+ +S+D   ++  L  N DE
Sbjct: 205 ENNKDEAPNLVIENESDD---EYSALNRNRDE 233

>sp|Q9JMH9|MY18A_MOUSE Myosin-18A (Myosin XVIIIa) (Myosin containing PDZ domain) (Molecule
            associated with JAK3 N-terminus) (MAJN)
          Length = 2035

 Score = 34.3 bits (77), Expect = 0.87
 Identities = 28/100 (28%), Positives = 51/100 (51%), Gaps = 7/100 (7%)
 Frame = +1

Query: 298  LQEIE---EFDDEYVPLPDNLPERSKEEMETLLWTQKRD----SQIREWQEVKQLEREKV 456
            ++E+E   EF+   V   +NL  R KE ME L  T++RD    ++ RE ++ K+L+R+  
Sbjct: 1820 IRELETRLEFEKTQVKRLENLASRLKETMEKL--TEERDQRAAAENREKEQNKRLQRQLR 1877

Query: 457  KLEHQVRSMQRRNSRAHKTSDILKVPPFAKPHHLAIDQDS 576
              + ++  + R+ + A +           K H L +D +S
Sbjct: 1878 DTKEEMSELARKEAEASR-----------KKHELEMDLES 1906

>sp|P08799|MYS2_DICDI Myosin II heavy chain, non muscle
          Length = 2116

 Score = 34.3 bits (77), Expect = 0.87
 Identities = 37/152 (24%), Positives = 72/152 (47%), Gaps = 8/152 (5%)
 Frame = +1

Query: 253  SSSIAKMAGIQNPDLLQEIEEFDDEYVPLPDNLPERSK-----EEMETLLWTQKRDSQIR 417
            S +I+++  I++ +L +E+EE       L ++  E SK     E+    L ++  D  +R
Sbjct: 967  SDTISRLEKIKD-ELQKEVEE-------LTESFSEESKDKGVLEKTRVRLQSELDDLTVR 1018

Query: 418  ---EWQEVKQLEREKVKLEHQVRSMQRRNSRAHKTSDILKVPPFAKPHHLAIDQDSEDKR 588
               E ++  +L R+K KLE +++ +Q     A       K+   A    L  +    +++
Sbjct: 1019 LDSETKDKSELLRQKKKLEEELKQVQ----EALAAETAAKLAQEAANKKLQGEYTELNEK 1074

Query: 589  FQFPLLTTNDDEKTMKTFNVQLSNFNDKLMEQ 684
            F   +   ++ EK+ KT   QL   N++L E+
Sbjct: 1075 FNSEVTARSNVEKSKKTLESQLVAVNNELDEE 1106

>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
          Length = 1790

 Score = 33.9 bits (76), Expect = 1.1
 Identities = 35/187 (18%), Positives = 83/187 (44%), Gaps = 3/187 (1%)
 Frame = +1

Query: 169  DKSECIYDSKVKLSANCDLLIQNDWTSNSSSIAKMAGIQNPDLLQEIEEFDDE---YVPL 339
            D S+  Y+S++ L       ++   T+N  ++ K++     +L +  EE + E   Y  L
Sbjct: 1046 DSSKDEYESQISLLKE---KLETATTANDENVNKIS-----ELTKTREELEAELAAYKNL 1097

Query: 340  PDNLPERSKEEMETLLWTQKRDSQIREWQEVKQLEREKVKLEHQVRSMQRRNSRAHKTSD 519
             + L  + +   + L   ++ +  ++E  E  QLE+E  + + Q+ S++       K  +
Sbjct: 1098 KNELETKLETSEKALKEVKENEEHLKE--EKIQLEKEATETKQQLNSLRANLESLEKEHE 1155

Query: 520  ILKVPPFAKPHHLAIDQDSEDKRFQFPLLTTNDDEKTMKTFNVQLSNFNDKLMEQINIVK 699
             L          +A    ++++++   +   ND+  + +  N  +   ND+L  ++  +K
Sbjct: 1156 DLAAQLKKYEEQIA----NKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVKAMK 1211

Query: 700  NNKTTKS 720
            +    +S
Sbjct: 1212 STSEEQS 1218

>sp|O67825|IF2_AQUAE Translation initiation factor IF-2
          Length = 805

 Score = 33.9 bits (76), Expect = 1.1
 Identities = 33/159 (20%), Positives = 72/159 (45%), Gaps = 1/159 (0%)
 Frame = +1

Query: 244 TSNSSSIAKMAGIQNPDLLQEIEEFDDEYVPLPDNLPERSKEEMETLLWTQKRDS-QIRE 420
           T   S +AK  G+++    +EI EF +EY P PD  P ++   ++        D+  I+E
Sbjct: 6   TKRVSDVAKELGVKS----KEIIEFLNEYYPRPDGKPWKASHGLDEQALEMIYDAFGIKE 61

Query: 421 WQEVKQLEREKVKLEHQVRSMQRRNSRAHKTSDILKVPPFAKPHHLAIDQDSEDKRFQFP 600
            +E +++  E+ +   +V   +++     +   + +V    KP    I ++ E+K+ +  
Sbjct: 62  EEEKEEVVTEQAQAPAEVE--EKKEEEKKEEVIVEEVVEEKKPE--VIVEEIEEKKEEEE 117

Query: 601 LLTTNDDEKTMKTFNVQLSNFNDKLMEQINIVKNNKTTK 717
                  +K+++    ++    +K  E+  + K  K  K
Sbjct: 118 KKEEEKPKKSVEELIKEILEKKEKEKEKKKVEKERKEEK 156

>sp|Q07847|VE1_HPV63 Replication protein E1
          Length = 618

 Score = 33.5 bits (75), Expect = 1.5
 Identities = 31/143 (21%), Positives = 57/143 (39%), Gaps = 10/143 (6%)
 Frame = +1

Query: 304 EIEEFDDEYVPLPDNLPERSKEEMETLLWTQKRDSQIREWQEVKQLEREKVKLEHQVRSM 483
           E+E+ +D Y    ++L  RS+ ++  LL             + +Q +   ++L H    +
Sbjct: 26  ELEDLEDTY----NSLFNRSESDISDLL------------DDTQQSQGNSLELFHLQEHL 69

Query: 484 QRRNSRAHKTSDILKVPPFAK----------PHHLAIDQDSEDKRFQFPLLTTNDDEKTM 633
           Q            L  PP A           P   +I     +K+ +  L T ND    +
Sbjct: 70  QNEQDLNTLKRKYLNSPPQASATETACNSLSPRLESITISQREKKARKQLFTQNDSGIEL 129

Query: 634 KTFNVQLSNFNDKLMEQINIVKN 702
                ++ N N+ L EQ++IV++
Sbjct: 130 SLCQDEVDNINEALQEQVDIVQS 152

>sp|P02566|MYO4_CAEEL Myosin-4 (Myosin heavy chain B) (MHC B) (Uncoordinated protein 54)
          Length = 1966

 Score = 32.3 bits (72), Expect = 3.3
 Identities = 26/110 (23%), Positives = 54/110 (49%), Gaps = 4/110 (3%)
 Frame = +1

Query: 169  DKSECIYDSKVKLSANCDLLIQNDWTSNSSSIAKMAGI--QNPDLLQEIEEFDDEYVPLP 342
            + S  + + K  L  N    +++  T  S +  ++A +  Q  D  +++ E +D+     
Sbjct: 887  ESSAKLVEEKTSLFTN----LESTKTQLSDAEERLAKLEAQQKDASKQLSELNDQLADNE 942

Query: 343  DNLPE--RSKEEMETLLWTQKRDSQIREWQEVKQLEREKVKLEHQVRSMQ 486
            D   +  R+K+++E  +   K+  Q  E   +++ E EK   +HQ+RS+Q
Sbjct: 943  DRTADVQRAKKKIEAEVEALKKQIQDLE-MSLRKAESEKQSKDHQIRSLQ 991

>sp|P36353|VNS5_MSTV Nonstructural protein NS5
          Length = 375

 Score = 32.3 bits (72), Expect = 3.3
 Identities = 28/108 (25%), Positives = 51/108 (47%), Gaps = 3/108 (2%)
 Frame = +1

Query: 151 DCHFDFDKSECIYDSKVKLSANCDLLIQNDWTSNSSSIAKMAGIQNP--DLLQEIEEFDD 324
           D  F + K  C +++  +    C      DW S   S+     +Q+P  DL+        
Sbjct: 242 DQEFRYWKGHCSFET-TRPRVRC-----GDWQSPLDSLV---ALQSPPMDLVCNAPTLLP 292

Query: 325 EYVPLPDNLPERSKEEMETLLWTQKRDSQIREWQE-VKQLEREKVKLE 465
             +P+PD+ P   K ++  L WT++ +  +RE Q+ +K+   EK K++
Sbjct: 293 TAIPVPDH-PSYRKYKLSDLSWTKQHERAVREDQKRLKRAVGEKDKIK 339

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 139,704,330
Number of Sequences: 369166
Number of extensions: 2772247
Number of successful extensions: 9197
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 8593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 9177
length of database: 68,354,980
effective HSP length: 114
effective length of database: 47,295,190
effective search space used: 16174954980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00214

  1. Dr_sW_006_E10
  2. Dr_sW_003_I20
  3. Dr_sW_027_F01