Planaria EST Database

DrC_00211

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00211
         (1544 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q03387|IF41_WHEAT  Eukaryotic initiation factor (iso)4F s...    53   2e-06
sp|P78344|IF4G2_HUMAN  Eukaryotic translation initiation fac...    50   1e-05
sp|Q95L46|I4G2_BOVIN  Eukaryotic translation initiation fact...    50   2e-05
sp|Q62448|IF4G2_MOUSE  Eukaryotic translation initiation fac...    46   3e-04
sp|P41110|IF4G1_RABIT  Eukaryotic translation initiation fac...    45   7e-04
sp|Q6NZJ6|IF4G1_MOUSE  Eukaryotic translation initiation fac...    44   0.002
sp|Q04637|IF4G1_HUMAN  Eukaryotic translation initiation fac...    44   0.002
sp|Q80XI3|IF4G3_MOUSE  Eukaryotic translation initiation fac...    36   0.35 
sp|Q6GYQ0|GRIPE_HUMAN  GTPase-activating Rap/Ran-GAP domain-...    33   2.2  
sp|Q9Y6W6|DUS10_HUMAN  Dual specificity protein phosphatase ...    33   2.9  

>sp|Q03387|IF41_WHEAT Eukaryotic initiation factor (iso)4F subunit p82-34 (eIF-(iso)4F
            p82-34)
          Length = 788

 Score = 53.1 bits (126), Expect = 2e-06
 Identities = 30/96 (31%), Positives = 51/96 (53%)
 Frame = +1

Query: 1030 LISKIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKL 1209
            L  K   LL EY       EA++C+ EL++P +H E+V +AI + ++K   +  + ++KL
Sbjct: 623  LQKKTASLLEEYFGIRILDEAQQCIEELQSPDYHPEIVKEAINLALDKGASFV-DPLVKL 681

Query: 1210 LDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAA 1317
            L+ L        + LE G +     + DI +D+P A
Sbjct: 682  LEHLYTKKTFKTEDLENGCLLYGSLLEDIGIDLPKA 717

>sp|P78344|IF4G2_HUMAN Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
            (eIF-4G 2) (eIF4G 2) (p97) (Death associated protein 5)
            (DAP-5)
          Length = 907

 Score = 50.4 bits (119), Expect = 1e-05
 Identities = 34/127 (26%), Positives = 62/127 (48%), Gaps = 1/127 (0%)
 Frame = +1

Query: 1030 LISKIRFLLAEYLDSDDEIEAERCLRELEAP-HFHHELVFQAILMVIEKSTEYACNQMIK 1206
            L+     ++ EYL+S +  EA   +RE+ AP HF  E++ + I++ +++S E    +   
Sbjct: 544  LLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDK-EKASS 602

Query: 1207 LLDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLA 1386
            L+  L +  I T D   Q  + + DQ P +++D+P     L +F   A  +      +LA
Sbjct: 603  LISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELA 662

Query: 1387 NKMPKNT 1407
              +   T
Sbjct: 663  QPLESGT 669

>sp|Q95L46|I4G2_BOVIN Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
            (eIF-4G 2) (eIF4G 2) (p97)
          Length = 136

 Score = 49.7 bits (117), Expect = 2e-05
 Identities = 30/104 (28%), Positives = 55/104 (52%), Gaps = 1/104 (0%)
 Frame = +1

Query: 1030 LISKIRFLLAEYLDSDDEIEAERCLRELEAP-HFHHELVFQAILMVIEKSTEYACNQMIK 1206
            L+     ++ EYL+S +  EA   +RE+ AP HF  E++ + I++ +++S E    +   
Sbjct: 31   LLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDK-EKASS 89

Query: 1207 LLDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERF 1338
            L+  L +  I T D   Q  + + DQ P +++D+P     L +F
Sbjct: 90   LISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQF 133

>sp|Q62448|IF4G2_MOUSE Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
            (eIF-4G 2) (eIF4G 2) (p97) (Novel APOBEC-1 target 1)
            (Translation repressor NAT1)
          Length = 906

 Score = 46.2 bits (108), Expect = 3e-04
 Identities = 31/127 (24%), Positives = 62/127 (48%), Gaps = 1/127 (0%)
 Frame = +1

Query: 1030 LISKIRFLLAEYLDSDDEIEAERCLRELEAP-HFHHELVFQAILMVIEKSTEYACNQMIK 1206
            L+     ++ +YL+S +  +A   +RE+ AP HF  E++ + I++ +++S E    +   
Sbjct: 543  LLKLTEAVVTDYLNSGNANDAVSGVREMRAPKHFLPEMLSKVIILSLDRSDEDK-EKASS 601

Query: 1207 LLDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLA 1386
            L+  L +  I T D   Q  + + +Q P +++D+P     L +F   A  +      +LA
Sbjct: 602  LISLLKQEGIATSDNFMQAFLNVLEQCPKLEVDIPLVKSYLAQFAARAIISELVSISELA 661

Query: 1387 NKMPKNT 1407
              +   T
Sbjct: 662  QPLESGT 668

>sp|P41110|IF4G1_RABIT Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1)
            (eIF-4G1) (eIF-4G 1) (p220)
          Length = 1402

 Score = 44.7 bits (104), Expect = 7e-04
 Identities = 32/127 (25%), Positives = 61/127 (48%)
 Frame = +1

Query: 1030 LISKIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKL 1209
            L  K + ++ EYL  +D  EA +C++EL +P      V   I   +E+S   A  +M +L
Sbjct: 1045 LEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSA-IARERMGQL 1103

Query: 1210 LDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLAN 1389
            L  L  +  ++  Q  QG+  I +   D+++D+P  ++ L   +    +       +L  
Sbjct: 1104 LHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPIMQEGGVPMGELFR 1163

Query: 1390 KMPKNTR 1410
            ++ K+ R
Sbjct: 1164 EITKHLR 1170

>sp|Q6NZJ6|IF4G1_MOUSE Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1)
            (eIF-4G1) (eIF-4G 1)
          Length = 1600

 Score = 43.5 bits (101), Expect = 0.002
 Identities = 29/101 (28%), Positives = 51/101 (50%)
 Frame = +1

Query: 1039 KIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKLLDS 1218
            K + ++ EYL  +D  EA +C++EL +P      V   I   +E+ST  A   M +LL  
Sbjct: 1245 KSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERST-IAREHMGRLLHQ 1303

Query: 1219 LCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFI 1341
            L  +  ++  Q  QG+    +   D+++D+P  ++ L   I
Sbjct: 1304 LLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELI 1344

>sp|Q04637|IF4G1_HUMAN Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1)
            (eIF-4G1) (eIF-4G 1) (p220)
          Length = 1600

 Score = 43.5 bits (101), Expect = 0.002
 Identities = 28/104 (26%), Positives = 52/104 (50%)
 Frame = +1

Query: 1030 LISKIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKL 1209
            L  K + ++ EYL  +D  EA +C++EL +P      V   +   +E+S   A   M +L
Sbjct: 1243 LEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSA-IAREHMGQL 1301

Query: 1210 LDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFI 1341
            L  L  +  ++  Q  QG+  I +   D+++D+P  ++ L   +
Sbjct: 1302 LHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELV 1345

>sp|Q80XI3|IF4G3_MOUSE Eukaryotic translation initiation factor 4 gamma 3 (eIF-4-gamma 3)
            (eIF-4G 3) (eIF4G 3) (eIF-4-gamma II) (eIF4GII)
          Length = 1579

 Score = 35.8 bits (81), Expect = 0.35
 Identities = 27/121 (22%), Positives = 53/121 (43%)
 Frame = +1

Query: 1039 KIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKLLDS 1218
            K + ++ E+L  +D  EA +C+ EL A    H  V   +   +E+S +   + M  LL  
Sbjct: 1219 KSKSIIDEFLHINDFKEATQCIEELSAQGPLHVFVKVGVEFTLERS-QITRDHMGHLLYQ 1277

Query: 1219 LCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLANKMP 1398
            L +S  ++     +G     +   D+ +D+P  ++ L   +    K      ++L  +  
Sbjct: 1278 LVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELIVEFS 1337

Query: 1399 K 1401
            K
Sbjct: 1338 K 1338

>sp|Q6GYQ0|GRIPE_HUMAN GTPase-activating Rap/Ran-GAP domain-like 1 (GAP-related-interacting
            partner to E12) (GRIPE) (Tuberin-like protein 1)
          Length = 2036

 Score = 33.1 bits (74), Expect = 2.2
 Identities = 18/61 (29%), Positives = 33/61 (54%), Gaps = 2/61 (3%)
 Frame = +1

Query: 94   PRTVSFIGDVDDAPVVN--ELVAPQPLFAKESLENSAFKPIKNKKSKRQHSLSCSAASGI 267
            P ++S +  VD  P ++   L  P+PL + +S  +S  +P+   K++ QH L   AA  +
Sbjct: 1450 PMSLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIAARTV 1509

Query: 268  L 270
            +
Sbjct: 1510 I 1510

>sp|Q9Y6W6|DUS10_HUMAN Dual specificity protein phosphatase 10 (Mitogen-activated protein
            kinase phosphatase 5) (MAP kinase phosphatase 5) (MKP-5)
          Length = 482

 Score = 32.7 bits (73), Expect = 2.9
 Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
 Frame = +1

Query: 1369 YPKDLANKMPKNTRSRFLSEGDVFIQKRR-MEYDY*HLFASY*LN 1500
            YP DLA KM K ++S   S+G V I  R  MEY+  H+  +  +N
Sbjct: 151  YPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHIN 195

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 155,857,677
Number of Sequences: 369166
Number of extensions: 2963410
Number of successful extensions: 7081
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6748
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7080
length of database: 68,354,980
effective HSP length: 115
effective length of database: 47,110,455
effective search space used: 18797071545
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00211

  1. Dr_sW_009_P18
  2. Dr_sW_006_D08
  3. Dr_sW_006_M21
  4. Dr_sW_004_H14