Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_M21
(861 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q03387|IF41_WHEAT Eukaryotic initiation factor (iso)4F s... 53 1e-06
sp|P78344|IF4G2_HUMAN Eukaryotic translation initiation fac... 50 6e-06
sp|Q95L46|I4G2_BOVIN Eukaryotic translation initiation fact... 50 1e-05
sp|Q62448|IF4G2_MOUSE Eukaryotic translation initiation fac... 46 1e-04
sp|P41110|IF4G1_RABIT Eukaryotic translation initiation fac... 45 3e-04
sp|Q6NZJ6|IF4G1_MOUSE Eukaryotic translation initiation fac... 44 8e-04
sp|Q04637|IF4G1_HUMAN Eukaryotic translation initiation fac... 44 8e-04
sp|P79398|IF4G2_RABIT Eukaryotic translation initiation fac... 38 0.032
sp|Q80XI3|IF4G3_MOUSE Eukaryotic translation initiation fac... 36 0.16
sp|P12174|MATK_MARPO Maturase K (Intron maturase) 34 0.60
>sp|Q03387|IF41_WHEAT Eukaryotic initiation factor (iso)4F subunit p82-34 (eIF-(iso)4F
p82-34)
Length = 788
Score = 53.1 bits (126), Expect = 1e-06
Identities = 30/96 (31%), Positives = 51/96 (53%)
Frame = +2
Query: 434 LISKIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKL 613
L K LL EY EA++C+ EL++P +H E+V +AI + ++K + + ++KL
Sbjct: 623 LQKKTASLLEEYFGIRILDEAQQCIEELQSPDYHPEIVKEAINLALDKGASFV-DPLVKL 681
Query: 614 LDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAA 721
L+ L + LE G + + DI +D+P A
Sbjct: 682 LEHLYTKKTFKTEDLENGCLLYGSLLEDIGIDLPKA 717
>sp|P78344|IF4G2_HUMAN Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
(eIF-4G 2) (eIF4G 2) (p97) (Death associated protein 5)
(DAP-5)
Length = 907
Score = 50.4 bits (119), Expect = 6e-06
Identities = 34/127 (26%), Positives = 62/127 (48%), Gaps = 1/127 (0%)
Frame = +2
Query: 434 LISKIRFLLAEYLDSDDEIEAERCLRELEAP-HFHHELVFQAILMVIEKSTEYACNQMIK 610
L+ ++ EYL+S + EA +RE+ AP HF E++ + I++ +++S E +
Sbjct: 544 LLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDK-EKASS 602
Query: 611 LLDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLA 790
L+ L + I T D Q + + DQ P +++D+P L +F A + +LA
Sbjct: 603 LISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELA 662
Query: 791 NKMPKNT 811
+ T
Sbjct: 663 QPLESGT 669
Score = 35.8 bits (81), Expect = 0.16
Identities = 23/85 (27%), Positives = 44/85 (51%)
Frame = +2
Query: 26 NLVPELRSRIPYLCIVQALQRKPAQCELTSRLLCELIGTTINDDNMAKGFDLVISEMSEL 205
+ +PE+ S++ L + ++ + K L S L E I T+ DN + F V+ + +L
Sbjct: 576 HFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATS---DNFMQAFLNVLDQCPKL 632
Query: 206 TIDCPEAPEVVGKFFARAVADDLLA 280
+D P + +F ARA+ +L++
Sbjct: 633 EVDIPLVKSYLAQFAARAIISELVS 657
>sp|Q95L46|I4G2_BOVIN Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
(eIF-4G 2) (eIF4G 2) (p97)
Length = 136
Score = 49.7 bits (117), Expect = 1e-05
Identities = 30/104 (28%), Positives = 55/104 (52%), Gaps = 1/104 (0%)
Frame = +2
Query: 434 LISKIRFLLAEYLDSDDEIEAERCLRELEAP-HFHHELVFQAILMVIEKSTEYACNQMIK 610
L+ ++ EYL+S + EA +RE+ AP HF E++ + I++ +++S E +
Sbjct: 31 LLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDK-EKASS 89
Query: 611 LLDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERF 742
L+ L + I T D Q + + DQ P +++D+P L +F
Sbjct: 90 LISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQF 133
Score = 30.4 bits (67), Expect = 6.6
Identities = 21/77 (27%), Positives = 38/77 (49%)
Frame = +2
Query: 26 NLVPELRSRIPYLCIVQALQRKPAQCELTSRLLCELIGTTINDDNMAKGFDLVISEMSEL 205
+ +PE+ S++ L + ++ + K L S L E I T+ DN + F V+ + +L
Sbjct: 63 HFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATS---DNFMQAFLNVLDQCPKL 119
Query: 206 TIDCPEAPEVVGKFFAR 256
+D P + +F AR
Sbjct: 120 EVDIPLVKSYLAQFAAR 136
>sp|Q62448|IF4G2_MOUSE Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
(eIF-4G 2) (eIF4G 2) (p97) (Novel APOBEC-1 target 1)
(Translation repressor NAT1)
Length = 906
Score = 46.2 bits (108), Expect = 1e-04
Identities = 31/127 (24%), Positives = 62/127 (48%), Gaps = 1/127 (0%)
Frame = +2
Query: 434 LISKIRFLLAEYLDSDDEIEAERCLRELEAP-HFHHELVFQAILMVIEKSTEYACNQMIK 610
L+ ++ +YL+S + +A +RE+ AP HF E++ + I++ +++S E +
Sbjct: 543 LLKLTEAVVTDYLNSGNANDAVSGVREMRAPKHFLPEMLSKVIILSLDRSDEDK-EKASS 601
Query: 611 LLDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLA 790
L+ L + I T D Q + + +Q P +++D+P L +F A + +LA
Sbjct: 602 LISLLKQEGIATSDNFMQAFLNVLEQCPKLEVDIPLVKSYLAQFAARAIISELVSISELA 661
Query: 791 NKMPKNT 811
+ T
Sbjct: 662 QPLESGT 668
Score = 35.8 bits (81), Expect = 0.16
Identities = 23/85 (27%), Positives = 44/85 (51%)
Frame = +2
Query: 26 NLVPELRSRIPYLCIVQALQRKPAQCELTSRLLCELIGTTINDDNMAKGFDLVISEMSEL 205
+ +PE+ S++ L + ++ + K L S L E I T+ DN + F V+ + +L
Sbjct: 575 HFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATS---DNFMQAFLNVLEQCPKL 631
Query: 206 TIDCPEAPEVVGKFFARAVADDLLA 280
+D P + +F ARA+ +L++
Sbjct: 632 EVDIPLVKSYLAQFAARAIISELVS 656
>sp|P41110|IF4G1_RABIT Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1)
(eIF-4G1) (eIF-4G 1) (p220)
Length = 1402
Score = 44.7 bits (104), Expect = 3e-04
Identities = 32/127 (25%), Positives = 61/127 (48%)
Frame = +2
Query: 434 LISKIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKL 613
L K + ++ EYL +D EA +C++EL +P V I +E+S A +M +L
Sbjct: 1045 LEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGIESTLERSA-IARERMGQL 1103
Query: 614 LDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLAN 793
L L + ++ Q QG+ I + D+++D+P ++ L + + +L
Sbjct: 1104 LHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPIMQEGGVPMGELFR 1163
Query: 794 KMPKNTR 814
++ K+ R
Sbjct: 1164 EITKHLR 1170
>sp|Q6NZJ6|IF4G1_MOUSE Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1)
(eIF-4G1) (eIF-4G 1)
Length = 1600
Score = 43.5 bits (101), Expect = 8e-04
Identities = 29/101 (28%), Positives = 51/101 (50%)
Frame = +2
Query: 443 KIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKLLDS 622
K + ++ EYL +D EA +C++EL +P V I +E+ST A M +LL
Sbjct: 1245 KSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRLGIESTLERST-IAREHMGRLLHQ 1303
Query: 623 LCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFI 745
L + ++ Q QG+ + D+++D+P ++ L I
Sbjct: 1304 LLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELI 1344
>sp|Q04637|IF4G1_HUMAN Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1)
(eIF-4G1) (eIF-4G 1) (p220)
Length = 1600
Score = 43.5 bits (101), Expect = 8e-04
Identities = 28/104 (26%), Positives = 52/104 (50%)
Frame = +2
Query: 434 LISKIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKL 613
L K + ++ EYL +D EA +C++EL +P V + +E+S A M +L
Sbjct: 1243 LEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSA-IAREHMGQL 1301
Query: 614 LDSLCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFI 745
L L + ++ Q QG+ I + D+++D+P ++ L +
Sbjct: 1302 LHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELV 1345
>sp|P79398|IF4G2_RABIT Eukaryotic translation initiation factor 4 gamma 2 (eIF-4-gamma 2)
(eIF-4G 2) (eIF4G 2) (p97) (Novel APOBEC-1 target 1)
(Translation repressor NAT1)
Length = 907
Score = 38.1 bits (87), Expect = 0.032
Identities = 24/85 (28%), Positives = 45/85 (52%)
Frame = +2
Query: 26 NLVPELRSRIPYLCIVQALQRKPAQCELTSRLLCELIGTTINDDNMAKGFDLVISEMSEL 205
+ +PE+ S++ L + ++ + K L S L E IGT+ DN + F V+ + +L
Sbjct: 576 HFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIGTS---DNFMQAFLNVLDQCPKL 632
Query: 206 TIDCPEAPEVVGKFFARAVADDLLA 280
+D P + +F ARA+ +L++
Sbjct: 633 EVDIPLVKSYLAQFAARAIISELVS 657
>sp|Q80XI3|IF4G3_MOUSE Eukaryotic translation initiation factor 4 gamma 3 (eIF-4-gamma 3)
(eIF-4G 3) (eIF4G 3) (eIF-4-gamma II) (eIF4GII)
Length = 1579
Score = 35.8 bits (81), Expect = 0.16
Identities = 27/121 (22%), Positives = 53/121 (43%)
Frame = +2
Query: 443 KIRFLLAEYLDSDDEIEAERCLRELEAPHFHHELVFQAILMVIEKSTEYACNQMIKLLDS 622
K + ++ E+L +D EA +C+ EL A H V + +E+S + + M LL
Sbjct: 1219 KSKSIIDEFLHINDFKEATQCIEELSAQGPLHVFVKVGVEFTLERS-QITRDHMGHLLYQ 1277
Query: 623 LCKSVIVTIDQLEQGVMRIYDQMPDIQLDVPAAYILLERFINIATKNATFYPKDLANKMP 802
L +S ++ +G + D+ +D+P ++ L + K ++L +
Sbjct: 1278 LVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELIVEFS 1337
Query: 803 K 805
K
Sbjct: 1338 K 1338
>sp|P12174|MATK_MARPO Maturase K (Intron maturase)
Length = 370
Score = 33.9 bits (76), Expect = 0.60
Identities = 20/69 (28%), Positives = 38/69 (55%), Gaps = 1/69 (1%)
Frame = +2
Query: 14 LSELNLVP-ELRSRIPYLCIVQALQRKPAQCELTSRLLCELIGTTINDDNMAKGFDLVIS 190
L +NL+ E S IP + +++ L ++ C++ R LC+L TT++D+ + + FD +I
Sbjct: 213 LRNVNLIKKEFCSIIPVIPLIRLLAKEKF-CDVLGRPLCKLSWTTLSDNEIFERFDQIIK 271
Query: 191 EMSELTIDC 217
+ C
Sbjct: 272 HIFSYYSGC 280
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,473,169
Number of Sequences: 369166
Number of extensions: 1701680
Number of successful extensions: 4190
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4060
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4188
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)