Planarian EST Database

DrC_00109

Cluster detail
GO Category :
Consensus Sequence :
>DrC_00109 ATTCGGCACGAGGTTTTCTAGATAGAAAGCAGTGTAATCATAATTTCGGTTTTATTTTTA TCAGCAAAAACAGATCAAACATAAAAAATGACAGCCCTTTCTAAATCCGAAATTGAAGAT ATTCGTGAAGTTTTCGACCTTTTCGATTTCTGGGATGGTCGGGATGGTATGGTTGATGCA GTCAAAGTTGGAGATTTGTTGAGGTGTAACGGAATGAATCCTACCAATAGGATATGCATC AAACACGGTGCAACAGAGAAAATGGGGTTGAAACAGTACAAATTTGAAGAGTTTGTTCCG ATCTATGAAAAAATCATCCAAGAAAAGGAAAGTGGAACATTCGCAGATTATATGGAAGCC TTCAAAACCTTTGATCGTGAAGGACAGGGTTTCATTTCTCTTGCAGAAATGCGACACGTT TTGACTATGTACGGAGAGAAGTTGTCAGAAGAAGAAACAGATAACATTCTTAAATTTACT GACACTCATGAAGATATTGATGGTAACGTGAAATACGAAGATTTCATTAAAAAGGTTCTC GAAGGGCCGAAAACCAAGTAAAAATATCTGGATACCATAGTTATCAGAGACCTTTTCCAG TTCCAACATTGTTCTATCTGATTGCATGATGGCTACATTATTTTGCATGTGTTTATATTT TTGATGCGGTCAGTGTACATCCTGGAGCGTGATCAATTTAATCATAATTAATAGATATAA TCAATTGTGTAATTTTGCCTACCTTGTTTAATTTTGAAATATAACGATGGTCAAACAAT

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00109
         (779 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P07291|MLE_AEQIR  Myosin essential light chain, striated ...   206   6e-53
sp|P07290|MLE_PATYE  Myosin, essential light chain, adductor...   202   1e-51
sp|P05945|MLE_TODPA  Myosin catalytic light chain LC-1, mant...   201   2e-51
sp|P53014|MLE_CAEEL  Myosin, essential light chain (Myosin l...   129   9e-30
sp|P08296|MLEN_CHICK  Myosin light chain alkali, non-muscle ...   113   5e-25
sp|P02607|MLES_CHICK  Myosin light chain alkali, smooth-musc...   113   7e-25
sp|P14649|MLEY_HUMAN  Myosin light chain 1, slow-twitch musc...   112   1e-24
sp|P02605|MLE3_CHICK  Myosin light chain 3, skeletal muscle ...   110   3e-24
sp|P02604|MLE1_CHICK  Myosin light chain 1, skeletal muscle ...   109   7e-24
sp|Q17133|MLE_BRAFL  Myosin, essential light chain (Myosin l...   108   1e-23

>sp|P07291|MLE_AEQIR Myosin essential light chain, striated adductor muscle (E-LC)
           (Sulfhydryl light chain) (SHLC)
          Length = 157

 Score =  206 bits (524), Expect = 6e-53
 Identities = 98/154 (63%), Positives = 120/154 (77%)
 Frame = +1

Query: 88  MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMG 267
           M  LS+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N      G T KMG
Sbjct: 1   MPKLSQDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNEDVFAVGGTHKMG 60

Query: 268 LKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLS 447
            K   FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT  GE+LS
Sbjct: 61  EKSLPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLS 119

Query: 448 EEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 549
           +E+ D I+K TD  ED++GNVKYEDF+KKV+ GP
Sbjct: 120 DEDVDEIIKLTDLQEDLEGNVKYEDFVKKVMAGP 153

>sp|P07290|MLE_PATYE Myosin, essential light chain, adductor muscle (Sulfhydryl light
           chain) (SHLC)
          Length = 156

 Score =  202 bits (513), Expect = 1e-51
 Identities = 96/151 (63%), Positives = 118/151 (78%)
 Frame = +1

Query: 97  LSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQ 276
           LS+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N      G T KMG K 
Sbjct: 3   LSQDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNDDVFAVGGTHKMGEKS 62

Query: 277 YKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEE 456
             FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT  GE+L++EE
Sbjct: 63  LPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLTDEE 121

Query: 457 TDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 549
            D I+  TD  ED++GNVKYEDF+KKV+ GP
Sbjct: 122 IDEIISLTDLQEDLEGNVKYEDFVKKVMAGP 152

>sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mantle muscle
          Length = 160

 Score =  201 bits (510), Expect = 2e-51
 Identities = 98/152 (64%), Positives = 121/152 (79%), Gaps = 1/152 (0%)
 Frame = +1

Query: 97  LSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQ 276
           L+K EIE++REVFDLFDFWDGRDG VDA KVGDLLRC GMNPT     +HG T+KMG K 
Sbjct: 4   LTKDEIEEVREVFDLFDFWDGRDGDVDAAKVGDLLRCLGMNPTEAQVHQHGGTKKMGEKA 63

Query: 277 YKFEEFVPIYEKIIQEKESGTFAD-YMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 453
           YK EE +PIYE+ +  K++GT AD +MEAFKTFDREGQG IS AE+R+VL M GE+++E+
Sbjct: 64  YKLEEILPIYEE-MSSKDTGTAADEFMEAFKTFDREGQGLISSAEIRNVLKMLGERITED 122

Query: 454 ETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 549
           + ++I  F D  EDIDGN+KYED +KKV+ GP
Sbjct: 123 QCNDIFTFCDIREDIDGNIKYEDLMKKVMAGP 154

>sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin light chain alkali)
          Length = 153

 Score =  129 bits (324), Expect = 9e-30
 Identities = 67/145 (46%), Positives = 95/145 (65%), Gaps = 2/145 (1%)
 Frame = +1

Query: 121 IREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATE--KMGLKQYKFEEF 294
           ++E+F+L+D  +  DG +D  +VGD+ R  G+ PT  +  K    E  + G K+  FEE+
Sbjct: 9   LKEIFNLYD--EELDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQEFKRKGEKRLTFEEW 66

Query: 295 VPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILK 474
           +P+YE++ +EKE GT+AD+ E  K FD+E  G I  AE+RH+L   GE+LS +E D +LK
Sbjct: 67  LPMYEQLAKEKEQGTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLK 126

Query: 475 FTDTHEDIDGNVKYEDFIKKVLEGP 549
                ED +G VKYEDFIKKVL GP
Sbjct: 127 GV---EDGEGMVKYEDFIKKVLAGP 148

>sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle isoform (G2 catalytic)
           (LC17-NM)
          Length = 151

 Score =  113 bits (283), Expect = 5e-25
 Identities = 59/156 (37%), Positives = 93/156 (59%), Gaps = 3/156 (1%)
 Frame = +1

Query: 88  MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATE 258
           M   S+ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  ++
Sbjct: 1   MCDFSEEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSD 58

Query: 259 KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGE 438
           +M LK  KFE+F+P+ + I + K+ G F DY+E  + FD+EG G +  AE+RHVL   GE
Sbjct: 59  EMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGE 118

Query: 439 KLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 546
           K++EEE + ++     HED +G + YE F++ +L G
Sbjct: 119 KMTEEEVEQLVA---GHEDSNGCINYEAFVRHILSG 151


 Score = 33.1 bits (74), Expect = 0.87
 Identities = 20/72 (27%), Positives = 34/72 (47%)
 Frame = +1

Query: 343 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 522
           A++ EAF+ FDR G G I  ++   V+   G+  +  E   +L    + E     +K+E 
Sbjct: 10  AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69

Query: 523 FIKKVLEGPKTK 558
           F+  +    K K
Sbjct: 70  FLPMMQTIAKNK 81

>sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-muscle isoform (G2 catalytic)
           (LC17-GI)
          Length = 151

 Score =  113 bits (282), Expect = 7e-25
 Identities = 59/156 (37%), Positives = 93/156 (59%), Gaps = 3/156 (1%)
 Frame = +1

Query: 88  MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATE 258
           M   S+ +  + +E F LFD     DG +   + GD++R  G NPTN   +K   +  ++
Sbjct: 1   MCDFSEEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSD 58

Query: 259 KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGE 438
           +M LK  KFE+F+P+ + I + K+ G F DY+E  + FD+EG G +  AE+RHVL   GE
Sbjct: 59  EMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGE 118

Query: 439 KLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 546
           K++EEE + ++     HED +G + YE+ ++ VL G
Sbjct: 119 KMTEEEVEQLVA---GHEDSNGCINYEELVRMVLSG 151


 Score = 33.1 bits (74), Expect = 0.87
 Identities = 20/72 (27%), Positives = 34/72 (47%)
 Frame = +1

Query: 343 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 522
           A++ EAF+ FDR G G I  ++   V+   G+  +  E   +L    + E     +K+E 
Sbjct: 10  AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69

Query: 523 FIKKVLEGPKTK 558
           F+  +    K K
Sbjct: 70  FLPMMQTIAKNK 81

>sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch muscle A isoform (MLC1sa)
           (Alkali)
          Length = 208

 Score =  112 bits (279), Expect = 1e-24
 Identities = 56/150 (37%), Positives = 94/150 (62%), Gaps = 3/150 (2%)
 Frame = +1

Query: 100 SKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGL 270
           +K ++E+ +E F+LFD     DG +   + GD++R  G NPTN   +K   +  ++++  
Sbjct: 62  NKDQLEEFKEAFELFDRVG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKS 119

Query: 271 KQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSE 450
           ++  FE F+P+ + + + +  GT+ DY+E F+ FD+EG G +  AE+RHVLT  GEK++E
Sbjct: 120 RRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTE 179

Query: 451 EETDNILKFTDTHEDIDGNVKYEDFIKKVL 540
           EE + +L     HED +G + YE F+K +L
Sbjct: 180 EEVETVLA---GHEDSNGCINYEAFLKHIL 206


 Score = 32.7 bits (73), Expect = 1.1
 Identities = 19/73 (26%), Positives = 33/73 (45%)
 Frame = +1

Query: 310 KIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTH 489
           K++ E       ++ EAF+ FDR G G I  ++   V+   G+  +  E   +L    + 
Sbjct: 56  KVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSD 115

Query: 490 EDIDGNVKYEDFI 528
           E     V +E F+
Sbjct: 116 ELKSRRVDFETFL 128

>sp|P02605|MLE3_CHICK Myosin light chain 3, skeletal muscle isoform (A2 catalytic)
           (Alkali myosin light chain 3) (MLC-3) (Myosin light
           chain 3f) (Skeletal-muscle myosin L-4 light chain)
          Length = 150

 Score =  110 bits (276), Expect = 3e-24
 Identities = 59/152 (38%), Positives = 89/152 (58%), Gaps = 3/152 (1%)
 Frame = +1

Query: 94  ALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKM 264
           + S  EI D +E F LFD     D  +   +VGD++R  G NPTN    K   + + E+M
Sbjct: 2   SFSPDEINDFKEAFLLFDRTG--DAKITLSQVGDIVRALGQNPTNAEINKILGNPSKEEM 59

Query: 265 GLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKL 444
             K+  FEEF+P+ +     K+ GTF D++E  + FD+EG G +  AE+RHVL   GEK+
Sbjct: 60  NAKKITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKM 119

Query: 445 SEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 540
           +EEE + ++K     ED +G + YE F+K ++
Sbjct: 120 TEEEVEELMK---GQEDSNGCINYEAFVKHIM 148

>sp|P02604|MLE1_CHICK Myosin light chain 1, skeletal muscle isoform (A1 catalytic)
           (Alkali myosin light chain 1) (MLC-1) (Myosin light
           chain 1f) (Skeletal-muscle myosin L-1 light chain)
          Length = 192

 Score =  109 bits (273), Expect = 7e-24
 Identities = 58/150 (38%), Positives = 89/150 (59%), Gaps = 3/150 (2%)
 Frame = +1

Query: 100 SKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGL 270
           SK + +D +E F LFD     D  +   +VGD++R  G NPTN    K   + + E+M  
Sbjct: 46  SKEQQDDFKEAFLLFDRTG--DAKITLSQVGDIVRALGQNPTNAEINKILGNPSKEEMNA 103

Query: 271 KQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSE 450
           K+  FEEF+P+ +     K+ GTF D++E  + FD+EG G +  AE+RHVL   GEK++E
Sbjct: 104 KKITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTE 163

Query: 451 EETDNILKFTDTHEDIDGNVKYEDFIKKVL 540
           EE + ++K     ED +G + YE F+K ++
Sbjct: 164 EEVEELMK---GQEDSNGCINYEAFVKHIM 190

>sp|Q17133|MLE_BRAFL Myosin, essential light chain (Myosin light chain alkali)
          Length = 149

 Score =  108 bits (271), Expect = 1e-23
 Identities = 55/153 (35%), Positives = 94/153 (61%)
 Frame = +1

Query: 88  MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMG 267
           M  + +S I+++++ F LFD  +  DG +D  ++GD+LR  G+NP+N    K     +  
Sbjct: 1   MAEIEQSMIDEMKDGFPLFD--NKGDGKIDGAQLGDVLRSFGLNPSNAEVEKIAKANEG- 57

Query: 268 LKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLS 447
            K+  F++++ I+++++ + E G++ D+ E  K FD+EG G IS AE+RHVL   GEKL+
Sbjct: 58  -KRLSFDDYLAIHKQVLGQGEVGSYEDFFEGLKLFDKEGTGLISGAELRHVLATLGEKLT 116

Query: 448 EEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 546
           E + D ++      ED +GNV Y+ F K ++ G
Sbjct: 117 EAQVDELMAGGGGQEDAEGNVNYDTFAKYLMLG 149

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,316,158
Number of Sequences: 369166
Number of extensions: 1833324
Number of successful extensions: 5936
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5352
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5527
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7308943600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00109

Planaria EST Database

DrC_00109

BLASTX 2.2.13 [Nov-27-2005]

Cluster detail