Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_N08
(679 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07291|MLE_AEQIR Myosin essential light chain, striated ... 202 9e-52
sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mant... 199 7e-51
sp|P07290|MLE_PATYE Myosin, essential light chain, adductor... 199 7e-51
sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin l... 129 7e-30
sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch musc... 111 2e-24
sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle ... 111 2e-24
sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-musc... 110 3e-24
sp|P02605|MLE3_CHICK Myosin light chain 3, skeletal muscle ... 109 8e-24
sp|P02604|MLE1_CHICK Myosin light chain 1, skeletal muscle ... 108 2e-23
sp|Q60605|MYL6_MOUSE Myosin light polypeptide 6 (Myosin lig... 107 4e-23
>sp|P07291|MLE_AEQIR Myosin essential light chain, striated adductor muscle (E-LC)
(Sulfhydryl light chain) (SHLC)
Length = 157
Score = 202 bits (513), Expect = 9e-52
Identities = 95/149 (63%), Positives = 117/149 (78%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYK 182
+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N G T KMG K
Sbjct: 6 QDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNEDVFAVGGTHKMGEKSLP 65
Query: 183 FEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETD 362
FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT GE+LS+E+ D
Sbjct: 66 FEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLSDEDVD 124
Query: 363 NILKFTDTHEDIDGNVKYEDFIKKVLEGP 449
I+K TD ED++GNVKYEDF+KKV+ GP
Sbjct: 125 EIIKLTDLQEDLEGNVKYEDFVKKVMAGP 153
>sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mantle muscle
Length = 160
Score = 199 bits (505), Expect = 7e-51
Identities = 97/150 (64%), Positives = 119/150 (79%), Gaps = 1/150 (0%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYK 182
K EIE++REVFDLFDFWDGRDG VDA KVGDLLRC GMNPT +HG T+KMG K YK
Sbjct: 6 KDEIEEVREVFDLFDFWDGRDGDVDAAKVGDLLRCLGMNPTEAQVHQHGGTKKMGEKAYK 65
Query: 183 FEEFVPIYEKIIQEKESGTFAD-YMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEET 359
EE +PIYE+ + K++GT AD +MEAFKTFDREGQG IS AE+R+VL M GE+++E++
Sbjct: 66 LEEILPIYEE-MSSKDTGTAADEFMEAFKTFDREGQGLISSAEIRNVLKMLGERITEDQC 124
Query: 360 DNILKFTDTHEDIDGNVKYEDFIKKVLEGP 449
++I F D EDIDGN+KYED +KKV+ GP
Sbjct: 125 NDIFTFCDIREDIDGNIKYEDLMKKVMAGP 154
>sp|P07290|MLE_PATYE Myosin, essential light chain, adductor muscle (Sulfhydryl light
chain) (SHLC)
Length = 156
Score = 199 bits (505), Expect = 7e-51
Identities = 94/149 (63%), Positives = 116/149 (77%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQYK 182
+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N G T KMG K
Sbjct: 5 QDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNDDVFAVGGTHKMGEKSLP 64
Query: 183 FEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETD 362
FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT GE+L++EE D
Sbjct: 65 FEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLTDEEID 123
Query: 363 NILKFTDTHEDIDGNVKYEDFIKKVLEGP 449
I+ TD ED++GNVKYEDF+KKV+ GP
Sbjct: 124 EIISLTDLQEDLEGNVKYEDFVKKVMAGP 152
>sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin light chain alkali)
Length = 153
Score = 129 bits (324), Expect = 7e-30
Identities = 67/145 (46%), Positives = 95/145 (65%), Gaps = 2/145 (1%)
Frame = +3
Query: 21 IREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATE--KMGLKQYKFEEF 194
++E+F+L+D + DG +D +VGD+ R G+ PT + K E + G K+ FEE+
Sbjct: 9 LKEIFNLYD--EELDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQEFKRKGEKRLTFEEW 66
Query: 195 VPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILK 374
+P+YE++ +EKE GT+AD+ E K FD+E G I AE+RH+L GE+LS +E D +LK
Sbjct: 67 LPMYEQLAKEKEQGTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLK 126
Query: 375 FTDTHEDIDGNVKYEDFIKKVLEGP 449
ED +G VKYEDFIKKVL GP
Sbjct: 127 GV---EDGEGMVKYEDFIKKVLAGP 148
>sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch muscle A isoform (MLC1sa)
(Alkali)
Length = 208
Score = 111 bits (278), Expect = 2e-24
Identities = 56/149 (37%), Positives = 93/149 (62%), Gaps = 3/149 (2%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGLK 173
K ++E+ +E F+LFD DG + + GD++R G NPTN +K + ++++ +
Sbjct: 63 KDQLEEFKEAFELFDRVG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSR 120
Query: 174 QYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 353
+ FE F+P+ + + + + GT+ DY+E F+ FD+EG G + AE+RHVLT GEK++EE
Sbjct: 121 RVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEE 180
Query: 354 ETDNILKFTDTHEDIDGNVKYEDFIKKVL 440
E + +L HED +G + YE F+K +L
Sbjct: 181 EVETVLA---GHEDSNGCINYEAFLKHIL 206
Score = 32.7 bits (73), Expect = 0.90
Identities = 19/73 (26%), Positives = 33/73 (45%)
Frame = +3
Query: 210 KIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTH 389
K++ E ++ EAF+ FDR G G I ++ V+ G+ + E +L +
Sbjct: 56 KVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSD 115
Query: 390 EDIDGNVKYEDFI 428
E V +E F+
Sbjct: 116 ELKSRRVDFETFL 128
>sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle isoform (G2 catalytic)
(LC17-NM)
Length = 151
Score = 111 bits (277), Expect = 2e-24
Identities = 57/151 (37%), Positives = 91/151 (60%), Gaps = 3/151 (1%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGLK 173
+ + + +E F LFD DG + + GD++R G NPTN +K + +++M LK
Sbjct: 6 EEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLK 63
Query: 174 QYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 353
KFE+F+P+ + I + K+ G F DY+E + FD+EG G + AE+RHVL GEK++EE
Sbjct: 64 TLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEE 123
Query: 354 ETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 446
E + ++ HED +G + YE F++ +L G
Sbjct: 124 EVEQLVA---GHEDSNGCINYEAFVRHILSG 151
Score = 33.1 bits (74), Expect = 0.69
Identities = 20/72 (27%), Positives = 34/72 (47%)
Frame = +3
Query: 243 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 422
A++ EAF+ FDR G G I ++ V+ G+ + E +L + E +K+E
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69
Query: 423 FIKKVLEGPKTK 458
F+ + K K
Sbjct: 70 FLPMMQTIAKNK 81
>sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-muscle isoform (G2 catalytic)
(LC17-GI)
Length = 151
Score = 110 bits (276), Expect = 3e-24
Identities = 57/151 (37%), Positives = 91/151 (60%), Gaps = 3/151 (1%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGLK 173
+ + + +E F LFD DG + + GD++R G NPTN +K + +++M LK
Sbjct: 6 EEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLK 63
Query: 174 QYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 353
KFE+F+P+ + I + K+ G F DY+E + FD+EG G + AE+RHVL GEK++EE
Sbjct: 64 TLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEE 123
Query: 354 ETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 446
E + ++ HED +G + YE+ ++ VL G
Sbjct: 124 EVEQLVA---GHEDSNGCINYEELVRMVLSG 151
Score = 33.1 bits (74), Expect = 0.69
Identities = 20/72 (27%), Positives = 34/72 (47%)
Frame = +3
Query: 243 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 422
A++ EAF+ FDR G G I ++ V+ G+ + E +L + E +K+E
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69
Query: 423 FIKKVLEGPKTK 458
F+ + K K
Sbjct: 70 FLPMMQTIAKNK 81
>sp|P02605|MLE3_CHICK Myosin light chain 3, skeletal muscle isoform (A2 catalytic)
(Alkali myosin light chain 3) (MLC-3) (Myosin light
chain 3f) (Skeletal-muscle myosin L-4 light chain)
Length = 150
Score = 109 bits (272), Expect = 8e-24
Identities = 58/147 (39%), Positives = 87/147 (59%), Gaps = 3/147 (2%)
Frame = +3
Query: 9 EIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGLKQY 179
EI D +E F LFD D + +VGD++R G NPTN K + + E+M K+
Sbjct: 7 EINDFKEAFLLFDRTG--DAKITLSQVGDIVRALGQNPTNAEINKILGNPSKEEMNAKKI 64
Query: 180 KFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEET 359
FEEF+P+ + K+ GTF D++E + FD+EG G + AE+RHVL GEK++EEE
Sbjct: 65 TFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEEEV 124
Query: 360 DNILKFTDTHEDIDGNVKYEDFIKKVL 440
+ ++K ED +G + YE F+K ++
Sbjct: 125 EELMK---GQEDSNGCINYEAFVKHIM 148
>sp|P02604|MLE1_CHICK Myosin light chain 1, skeletal muscle isoform (A1 catalytic)
(Alkali myosin light chain 1) (MLC-1) (Myosin light
chain 1f) (Skeletal-muscle myosin L-1 light chain)
Length = 192
Score = 108 bits (269), Expect = 2e-23
Identities = 57/149 (38%), Positives = 88/149 (59%), Gaps = 3/149 (2%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGLK 173
K + +D +E F LFD D + +VGD++R G NPTN K + + E+M K
Sbjct: 47 KEQQDDFKEAFLLFDRTG--DAKITLSQVGDIVRALGQNPTNAEINKILGNPSKEEMNAK 104
Query: 174 QYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 353
+ FEEF+P+ + K+ GTF D++E + FD+EG G + AE+RHVL GEK++EE
Sbjct: 105 KITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEE 164
Query: 354 ETDNILKFTDTHEDIDGNVKYEDFIKKVL 440
E + ++K ED +G + YE F+K ++
Sbjct: 165 EVEELMK---GQEDSNGCINYEAFVKHIM 190
>sp|Q60605|MYL6_MOUSE Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
sp|P60661|MYL6_BOVIN Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
sp|P60662|MYL6_PIG Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
sp|P60660|MYL6_HUMAN Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
Length = 151
Score = 107 bits (266), Expect = 4e-23
Identities = 54/151 (35%), Positives = 90/151 (59%), Gaps = 3/151 (1%)
Frame = +3
Query: 3 KSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGLK 173
+ + + +E F LFD DG + + GD++R G NPTN +K + +++M +K
Sbjct: 6 EDQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVK 63
Query: 174 QYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 353
FE F+P+ + + + K+ GT+ DY+E + FD+EG G + AE+RHVL GEK++EE
Sbjct: 64 VLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEE 123
Query: 354 ETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 446
E + ++ HED +G + YE F++ +L G
Sbjct: 124 EVEMLVA---GHEDSNGCINYEAFVRHILSG 151
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,581,789
Number of Sequences: 369166
Number of extensions: 1703051
Number of successful extensions: 5735
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5342
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)