Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_B21
(561 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07291|MLE_AEQIR Myosin essential light chain, striated ... 145 5e-35
sp|P07290|MLE_PATYE Myosin, essential light chain, adductor... 142 4e-34
sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mant... 132 8e-31
sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin l... 104 2e-22
sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle ... 95 1e-19
sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-musc... 95 1e-19
sp|Q60605|MYL6_MOUSE Myosin light polypeptide 6 (Myosin lig... 91 2e-18
sp|Q64119|MYL6_RAT Myosin light polypeptide 6 (Myosin light... 90 3e-18
sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch musc... 90 3e-18
sp|Q24756|MLC1_DROVI Myosin light chain alkali 90 3e-18
>sp|P07291|MLE_AEQIR Myosin essential light chain, striated adductor muscle (E-LC)
(Sulfhydryl light chain) (SHLC)
Length = 157
Score = 145 bits (367), Expect = 5e-35
Identities = 71/111 (63%), Positives = 84/111 (75%)
Frame = +1
Query: 1 NPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFI 180
NP N G T KMG K FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFI
Sbjct: 44 NPRNEDVFAVGGTHKMGEKSLPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFI 102
Query: 181 SLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 333
S AE+RHVLT GE+LS+E+ D I+K TD ED++GNVKYEDF+KKV+ GP
Sbjct: 103 SGAELRHVLTALGERLSDEDVDEIIKLTDLQEDLEGNVKYEDFVKKVMAGP 153
>sp|P07290|MLE_PATYE Myosin, essential light chain, adductor muscle (Sulfhydryl light
chain) (SHLC)
Length = 156
Score = 142 bits (359), Expect = 4e-34
Identities = 70/111 (63%), Positives = 83/111 (74%)
Frame = +1
Query: 1 NPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFI 180
NP N G T KMG K FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFI
Sbjct: 43 NPRNDDVFAVGGTHKMGEKSLPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFI 101
Query: 181 SLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 333
S AE+RHVLT GE+L++EE D I+ TD ED++GNVKYEDF+KKV+ GP
Sbjct: 102 SGAELRHVLTALGERLTDEEIDEIISLTDLQEDLEGNVKYEDFVKKVMAGP 152
>sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mantle muscle
Length = 160
Score = 132 bits (331), Expect = 8e-31
Identities = 65/112 (58%), Positives = 85/112 (75%), Gaps = 1/112 (0%)
Frame = +1
Query: 1 NPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFAD-YMEAFKTFDREGQGF 177
NPT +HG T+KMG K YK EE +PIYE++ K++GT AD +MEAFKTFDREGQG
Sbjct: 44 NPTEAQVHQHGGTKKMGEKAYKLEEILPIYEEM-SSKDTGTAADEFMEAFKTFDREGQGL 102
Query: 178 ISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 333
IS AE+R+VL M GE+++E++ ++I F D EDIDGN+KYED +KKV+ GP
Sbjct: 103 ISSAEIRNVLKMLGERITEDQCNDIFTFCDIREDIDGNIKYEDLMKKVMAGP 154
>sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin light chain alkali)
Length = 153
Score = 104 bits (259), Expect = 2e-22
Identities = 55/112 (49%), Positives = 73/112 (65%), Gaps = 2/112 (1%)
Frame = +1
Query: 4 PTNRICIKHGATE--KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGF 177
PT + K E + G K+ FEE++P+YE++ +EKE GT+AD+ E K FD+E G
Sbjct: 40 PTQAMVTKAAGQEFKRKGEKRLTFEEWLPMYEQLAKEKEQGTYADFYEGLKVFDKEETGK 99
Query: 178 ISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 333
I AE+RH+L GE+LS +E D +LK ED +G VKYEDFIKKVL GP
Sbjct: 100 ILAAELRHILLALGERLSADEADELLKGV---EDGEGMVKYEDFIKKVLAGP 148
>sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle isoform (G2 catalytic)
(LC17-NM)
Length = 151
Score = 95.1 bits (235), Expect = 1e-19
Identities = 46/113 (40%), Positives = 72/113 (63%), Gaps = 3/113 (2%)
Frame = +1
Query: 1 NPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQ 171
NPTN +K + +++M LK KFE+F+P+ + I + K+ G F DY+E + FD+EG
Sbjct: 42 NPTNAEVMKVLGNPKSDEMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGN 101
Query: 172 GFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 330
G + AE+RHVL GEK++EEE + ++ HED +G + YE F++ +L G
Sbjct: 102 GTVMGAEIRHVLVTLGEKMTEEEVEQLVA---GHEDSNGCINYEAFVRHILSG 151
Score = 33.1 bits (74), Expect = 0.48
Identities = 20/72 (27%), Positives = 34/72 (47%)
Frame = +1
Query: 127 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 306
A++ EAF+ FDR G G I ++ V+ G+ + E +L + E +K+E
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69
Query: 307 FIKKVLEGPKTK 342
F+ + K K
Sbjct: 70 FLPMMQTIAKNK 81
>sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-muscle isoform (G2 catalytic)
(LC17-GI)
Length = 151
Score = 94.7 bits (234), Expect = 1e-19
Identities = 46/113 (40%), Positives = 72/113 (63%), Gaps = 3/113 (2%)
Frame = +1
Query: 1 NPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQ 171
NPTN +K + +++M LK KFE+F+P+ + I + K+ G F DY+E + FD+EG
Sbjct: 42 NPTNAEVMKVLGNPKSDEMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGN 101
Query: 172 GFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 330
G + AE+RHVL GEK++EEE + ++ HED +G + YE+ ++ VL G
Sbjct: 102 GTVMGAEIRHVLVTLGEKMTEEEVEQLVA---GHEDSNGCINYEELVRMVLSG 151
Score = 33.1 bits (74), Expect = 0.48
Identities = 20/72 (27%), Positives = 34/72 (47%)
Frame = +1
Query: 127 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 306
A++ EAF+ FDR G G I ++ V+ G+ + E +L + E +K+E
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69
Query: 307 FIKKVLEGPKTK 342
F+ + K K
Sbjct: 70 FLPMMQTIAKNK 81
>sp|Q60605|MYL6_MOUSE Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
sp|P60661|MYL6_BOVIN Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
sp|P60662|MYL6_PIG Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
sp|P60660|MYL6_HUMAN Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
Length = 151
Score = 90.9 bits (224), Expect = 2e-18
Identities = 43/113 (38%), Positives = 71/113 (62%), Gaps = 3/113 (2%)
Frame = +1
Query: 1 NPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQ 171
NPTN +K + +++M +K FE F+P+ + + + K+ GT+ DY+E + FD+EG
Sbjct: 42 NPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGN 101
Query: 172 GFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 330
G + AE+RHVL GEK++EEE + ++ HED +G + YE F++ +L G
Sbjct: 102 GTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEAFVRHILSG 151
Score = 33.1 bits (74), Expect = 0.48
Identities = 20/61 (32%), Positives = 31/61 (50%)
Frame = +1
Query: 127 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 306
A++ EAF+ FDR G G I ++ V+ G+ + E +LK + + NVK D
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAE---VLKVLGNPKSDEMNVKVLD 66
Query: 307 F 309
F
Sbjct: 67 F 67
>sp|Q64119|MYL6_RAT Myosin light polypeptide 6 (Myosin light chain alkali 3) (Myosin
light chain 3) (MLC-3) (LC17)
Length = 151
Score = 90.1 bits (222), Expect = 3e-18
Identities = 43/113 (38%), Positives = 71/113 (62%), Gaps = 3/113 (2%)
Frame = +1
Query: 1 NPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQ 171
NPTN +K + +++M +K FE F+P+ + + + K+ GT+ DY+E + FD+EG
Sbjct: 42 NPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGN 101
Query: 172 GFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 330
G + AE+RHVL GEK++EEE + ++ HED +G + YE+ ++ VL G
Sbjct: 102 GTVMGAEIRHVLVTLGEKMTEEEVEMLVA---GHEDSNGCINYEELLRMVLNG 151
Score = 33.1 bits (74), Expect = 0.48
Identities = 20/61 (32%), Positives = 31/61 (50%)
Frame = +1
Query: 127 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 306
A++ EAF+ FDR G G I ++ V+ G+ + E +LK + + NVK D
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAE---VLKVLGNPKSDEMNVKVLD 66
Query: 307 F 309
F
Sbjct: 67 F 67
>sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch muscle A isoform (MLC1sa)
(Alkali)
Length = 208
Score = 90.1 bits (222), Expect = 3e-18
Identities = 43/111 (38%), Positives = 71/111 (63%), Gaps = 3/111 (2%)
Frame = +1
Query: 1 NPTNRICIK---HGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQ 171
NPTN +K + ++++ ++ FE F+P+ + + + + GT+ DY+E F+ FD+EG
Sbjct: 99 NPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGN 158
Query: 172 GFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 324
G + AE+RHVLT GEK++EEE + +L HED +G + YE F+K +L
Sbjct: 159 GKVMGAELRHVLTTLGEKMTEEEVETVLA---GHEDSNGCINYEAFLKHIL 206
Score = 32.7 bits (73), Expect = 0.63
Identities = 19/73 (26%), Positives = 33/73 (45%)
Frame = +1
Query: 94 KIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTH 273
K++ E ++ EAF+ FDR G G I ++ V+ G+ + E +L +
Sbjct: 56 KVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSD 115
Query: 274 EDIDGNVKYEDFI 312
E V +E F+
Sbjct: 116 ELKSRRVDFETFL 128
>sp|Q24756|MLC1_DROVI Myosin light chain alkali
Length = 155
Score = 90.1 bits (222), Expect = 3e-18
Identities = 41/111 (36%), Positives = 67/111 (60%)
Frame = +1
Query: 1 NPTNRICIKHGATEKMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFI 180
NPT + K G T+K K+ K +EF+PIY ++ +EKE G + D++E K +D+E G +
Sbjct: 41 NPTLALIEKMGGTKKRNEKKIKLDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTM 100
Query: 181 SLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 333
LAE++H L GE L +E+ +N+ ED +G + Y FI++++ P
Sbjct: 101 LLAELQHALLALGESLDDEQVENLFADCMDPEDDEGFIPYSQFIQRLMSDP 151
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,845,410
Number of Sequences: 369166
Number of extensions: 1334347
Number of successful extensions: 4484
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4378
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4029688280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)