Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_M14
(738 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07291|MLE_AEQIR Myosin essential light chain, striated ... 206 5e-53
sp|P07290|MLE_PATYE Myosin, essential light chain, adductor... 202 1e-51
sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mant... 201 2e-51
sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin l... 129 8e-30
sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle ... 113 5e-25
sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-musc... 113 6e-25
sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch musc... 112 1e-24
sp|P02605|MLE3_CHICK Myosin light chain 3, skeletal muscle ... 110 3e-24
sp|P02604|MLE1_CHICK Myosin light chain 1, skeletal muscle ... 109 7e-24
sp|Q17133|MLE_BRAFL Myosin, essential light chain (Myosin l... 108 1e-23
>sp|P07291|MLE_AEQIR Myosin essential light chain, striated adductor muscle (E-LC)
(Sulfhydryl light chain) (SHLC)
Length = 157
Score = 206 bits (524), Expect = 5e-53
Identities = 98/154 (63%), Positives = 120/154 (77%)
Frame = +3
Query: 51 MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMG 230
M LS+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N G T KMG
Sbjct: 1 MPKLSQDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNEDVFAVGGTHKMG 60
Query: 231 LKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLS 410
K FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT GE+LS
Sbjct: 61 EKSLPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLS 119
Query: 411 EEETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 512
+E+ D I+K TD ED++GNVKYEDF+KKV+ GP
Sbjct: 120 DEDVDEIIKLTDLQEDLEGNVKYEDFVKKVMAGP 153
>sp|P07290|MLE_PATYE Myosin, essential light chain, adductor muscle (Sulfhydryl light
chain) (SHLC)
Length = 156
Score = 202 bits (513), Expect = 1e-51
Identities = 96/151 (63%), Positives = 118/151 (78%)
Frame = +3
Query: 60 LSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQ 239
LS+ EI+D+++VF+LFDFWDGRDG VDA K+GD+ RC G+NP N G T KMG K
Sbjct: 3 LSQDEIDDLKDVFELFDFWDGRDGAVDAFKLGDVCRCLGINPRNDDVFAVGGTHKMGEKS 62
Query: 240 YKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEE 419
FEEF+P YE ++ + E GTFADYMEAFKTFDREGQGFIS AE+RHVLT GE+L++EE
Sbjct: 63 LPFEEFLPAYEGLM-DCEQGTFADYMEAFKTFDREGQGFISGAELRHVLTALGERLTDEE 121
Query: 420 TDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 512
D I+ TD ED++GNVKYEDF+KKV+ GP
Sbjct: 122 IDEIISLTDLQEDLEGNVKYEDFVKKVMAGP 152
>sp|P05945|MLE_TODPA Myosin catalytic light chain LC-1, mantle muscle
Length = 160
Score = 201 bits (510), Expect = 2e-51
Identities = 98/152 (64%), Positives = 121/152 (79%), Gaps = 1/152 (0%)
Frame = +3
Query: 60 LSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMGLKQ 239
L+K EIE++REVFDLFDFWDGRDG VDA KVGDLLRC GMNPT +HG T+KMG K
Sbjct: 4 LTKDEIEEVREVFDLFDFWDGRDGDVDAAKVGDLLRCLGMNPTEAQVHQHGGTKKMGEKA 63
Query: 240 YKFEEFVPIYEKIIQEKESGTFAD-YMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEE 416
YK EE +PIYE+ + K++GT AD +MEAFKTFDREGQG IS AE+R+VL M GE+++E+
Sbjct: 64 YKLEEILPIYEE-MSSKDTGTAADEFMEAFKTFDREGQGLISSAEIRNVLKMLGERITED 122
Query: 417 ETDNILKFTDTHEDIDGNVKYEDFIKKVLEGP 512
+ ++I F D EDIDGN+KYED +KKV+ GP
Sbjct: 123 QCNDIFTFCDIREDIDGNIKYEDLMKKVMAGP 154
>sp|P53014|MLE_CAEEL Myosin, essential light chain (Myosin light chain alkali)
Length = 153
Score = 129 bits (324), Expect = 8e-30
Identities = 67/145 (46%), Positives = 95/145 (65%), Gaps = 2/145 (1%)
Frame = +3
Query: 84 IREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATE--KMGLKQYKFEEF 257
++E+F+L+D + DG +D +VGD+ R G+ PT + K E + G K+ FEE+
Sbjct: 9 LKEIFNLYD--EELDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQEFKRKGEKRLTFEEW 66
Query: 258 VPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILK 437
+P+YE++ +EKE GT+AD+ E K FD+E G I AE+RH+L GE+LS +E D +LK
Sbjct: 67 LPMYEQLAKEKEQGTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLK 126
Query: 438 FTDTHEDIDGNVKYEDFIKKVLEGP 512
ED +G VKYEDFIKKVL GP
Sbjct: 127 GV---EDGEGMVKYEDFIKKVLAGP 148
>sp|P08296|MLEN_CHICK Myosin light chain alkali, non-muscle isoform (G2 catalytic)
(LC17-NM)
Length = 151
Score = 113 bits (283), Expect = 5e-25
Identities = 59/156 (37%), Positives = 93/156 (59%), Gaps = 3/156 (1%)
Frame = +3
Query: 51 MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATE 221
M S+ + + +E F LFD DG + + GD++R G NPTN +K + ++
Sbjct: 1 MCDFSEEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSD 58
Query: 222 KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGE 401
+M LK KFE+F+P+ + I + K+ G F DY+E + FD+EG G + AE+RHVL GE
Sbjct: 59 EMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGE 118
Query: 402 KLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 509
K++EEE + ++ HED +G + YE F++ +L G
Sbjct: 119 KMTEEEVEQLVA---GHEDSNGCINYEAFVRHILSG 151
Score = 33.1 bits (74), Expect = 0.79
Identities = 20/72 (27%), Positives = 34/72 (47%)
Frame = +3
Query: 306 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 485
A++ EAF+ FDR G G I ++ V+ G+ + E +L + E +K+E
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69
Query: 486 FIKKVLEGPKTK 521
F+ + K K
Sbjct: 70 FLPMMQTIAKNK 81
>sp|P02607|MLES_CHICK Myosin light chain alkali, smooth-muscle isoform (G2 catalytic)
(LC17-GI)
Length = 151
Score = 113 bits (282), Expect = 6e-25
Identities = 59/156 (37%), Positives = 93/156 (59%), Gaps = 3/156 (1%)
Frame = +3
Query: 51 MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATE 221
M S+ + + +E F LFD DG + + GD++R G NPTN +K + ++
Sbjct: 1 MCDFSEEQTAEFKEAFQLFDRTG--DGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSD 58
Query: 222 KMGLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGE 401
+M LK KFE+F+P+ + I + K+ G F DY+E + FD+EG G + AE+RHVL GE
Sbjct: 59 EMNLKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGE 118
Query: 402 KLSEEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 509
K++EEE + ++ HED +G + YE+ ++ VL G
Sbjct: 119 KMTEEEVEQLVA---GHEDSNGCINYEELVRMVLSG 151
Score = 33.1 bits (74), Expect = 0.79
Identities = 20/72 (27%), Positives = 34/72 (47%)
Frame = +3
Query: 306 ADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTHEDIDGNVKYED 485
A++ EAF+ FDR G G I ++ V+ G+ + E +L + E +K+E
Sbjct: 10 AEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNLKTLKFEQ 69
Query: 486 FIKKVLEGPKTK 521
F+ + K K
Sbjct: 70 FLPMMQTIAKNK 81
>sp|P14649|MLEY_HUMAN Myosin light chain 1, slow-twitch muscle A isoform (MLC1sa)
(Alkali)
Length = 208
Score = 112 bits (279), Expect = 1e-24
Identities = 56/150 (37%), Positives = 94/150 (62%), Gaps = 3/150 (2%)
Frame = +3
Query: 63 SKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGL 233
+K ++E+ +E F+LFD DG + + GD++R G NPTN +K + ++++
Sbjct: 62 NKDQLEEFKEAFELFDRVG--DGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKS 119
Query: 234 KQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSE 413
++ FE F+P+ + + + + GT+ DY+E F+ FD+EG G + AE+RHVLT GEK++E
Sbjct: 120 RRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTE 179
Query: 414 EETDNILKFTDTHEDIDGNVKYEDFIKKVL 503
EE + +L HED +G + YE F+K +L
Sbjct: 180 EEVETVLA---GHEDSNGCINYEAFLKHIL 206
Score = 32.7 bits (73), Expect = 1.0
Identities = 19/73 (26%), Positives = 33/73 (45%)
Frame = +3
Query: 273 KIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSEEETDNILKFTDTH 452
K++ E ++ EAF+ FDR G G I ++ V+ G+ + E +L +
Sbjct: 56 KVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSD 115
Query: 453 EDIDGNVKYEDFI 491
E V +E F+
Sbjct: 116 ELKSRRVDFETFL 128
>sp|P02605|MLE3_CHICK Myosin light chain 3, skeletal muscle isoform (A2 catalytic)
(Alkali myosin light chain 3) (MLC-3) (Myosin light
chain 3f) (Skeletal-muscle myosin L-4 light chain)
Length = 150
Score = 110 bits (276), Expect = 3e-24
Identities = 59/152 (38%), Positives = 89/152 (58%), Gaps = 3/152 (1%)
Frame = +3
Query: 57 ALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKM 227
+ S EI D +E F LFD D + +VGD++R G NPTN K + + E+M
Sbjct: 2 SFSPDEINDFKEAFLLFDRTG--DAKITLSQVGDIVRALGQNPTNAEINKILGNPSKEEM 59
Query: 228 GLKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKL 407
K+ FEEF+P+ + K+ GTF D++E + FD+EG G + AE+RHVL GEK+
Sbjct: 60 NAKKITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKM 119
Query: 408 SEEETDNILKFTDTHEDIDGNVKYEDFIKKVL 503
+EEE + ++K ED +G + YE F+K ++
Sbjct: 120 TEEEVEELMK---GQEDSNGCINYEAFVKHIM 148
>sp|P02604|MLE1_CHICK Myosin light chain 1, skeletal muscle isoform (A1 catalytic)
(Alkali myosin light chain 1) (MLC-1) (Myosin light
chain 1f) (Skeletal-muscle myosin L-1 light chain)
Length = 192
Score = 109 bits (273), Expect = 7e-24
Identities = 58/150 (38%), Positives = 89/150 (59%), Gaps = 3/150 (2%)
Frame = +3
Query: 63 SKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIK---HGATEKMGL 233
SK + +D +E F LFD D + +VGD++R G NPTN K + + E+M
Sbjct: 46 SKEQQDDFKEAFLLFDRTG--DAKITLSQVGDIVRALGQNPTNAEINKILGNPSKEEMNA 103
Query: 234 KQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLSE 413
K+ FEEF+P+ + K+ GTF D++E + FD+EG G + AE+RHVL GEK++E
Sbjct: 104 KKITFEEFLPMLQAAANNKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTE 163
Query: 414 EETDNILKFTDTHEDIDGNVKYEDFIKKVL 503
EE + ++K ED +G + YE F+K ++
Sbjct: 164 EEVEELMK---GQEDSNGCINYEAFVKHIM 190
>sp|Q17133|MLE_BRAFL Myosin, essential light chain (Myosin light chain alkali)
Length = 149
Score = 108 bits (271), Expect = 1e-23
Identities = 55/153 (35%), Positives = 94/153 (61%)
Frame = +3
Query: 51 MTALSKSEIEDIREVFDLFDFWDGRDGMVDAVKVGDLLRCNGMNPTNRICIKHGATEKMG 230
M + +S I+++++ F LFD + DG +D ++GD+LR G+NP+N K +
Sbjct: 1 MAEIEQSMIDEMKDGFPLFD--NKGDGKIDGAQLGDVLRSFGLNPSNAEVEKIAKANEG- 57
Query: 231 LKQYKFEEFVPIYEKIIQEKESGTFADYMEAFKTFDREGQGFISLAEMRHVLTMYGEKLS 410
K+ F++++ I+++++ + E G++ D+ E K FD+EG G IS AE+RHVL GEKL+
Sbjct: 58 -KRLSFDDYLAIHKQVLGQGEVGSYEDFFEGLKLFDKEGTGLISGAELRHVLATLGEKLT 116
Query: 411 EEETDNILKFTDTHEDIDGNVKYEDFIKKVLEG 509
E + D ++ ED +GNV Y+ F K ++ G
Sbjct: 117 EAQVDELMAGGGGQEDAEGNVNYDTFAKYLMLG 149
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,622,957
Number of Sequences: 369166
Number of extensions: 1777378
Number of successful extensions: 5875
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5290
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5466
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6631293200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)