Planaria EST Database


DrC_00100

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00100
         (747 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P59282|P25B_HUMAN  Protein p25-beta                             66   1e-10
sp|Q9BW30|CG38_HUMAN  Protein CGI-38                               65   1e-10
sp|Q9CRB6|CG38_MOUSE  Protein CGI-38 homolog                       63   7e-10
sp|Q7TQD2|P25A_MOUSE  Tubulin polymerization-promoting prote...    60   5e-09
sp|Q27957|P25A_BOVIN  Tubulin polymerization-promoting prote...    58   3e-08
sp|O94811|P25A_HUMAN  Tubulin polymerization-promoting prote...    56   9e-08
sp|P91127|YBYK_CAEEL  Hypothetical protein C32E8.3 in chromo...    53   8e-07
sp|Q9VV43|Y4893_DROME  Hypothetical protein CG4893                 52   2e-06
sp|P32350|KNS1_YEAST  Protein kinase KNS1                          32   1.8  
sp|O46409|APOA4_PIG  Apolipoprotein A-IV precursor (Apo-AIV)...    31   4.0  
>sp|P59282|P25B_HUMAN Protein p25-beta
          Length = 170

 Score = 65.9 bits (159), Expect = 1e-10
 Identities = 50/149 (33%), Positives = 66/149 (44%), Gaps = 5/149 (3%)
 Frame = +3

Query: 168 FLEFCESTKKGSTTATDKTIKKICTDAKLYT-KTFTANDVDIEFRKCIGNQKKDVDFAGF 344
           F  F ES+  G T   +K   K+C D  +   KT T+ DVDI F K      + + F  F
Sbjct: 12  FAAFGESSSSG-TEMNNKNFSKLCKDCGIMDGKTVTSTDVDIVFSKVKAKNARTITFQQF 70

Query: 345 ENFLDKFFIPAYAKSCKKDAAAISDEVKKKIIS----NGPKAHGATKASKDGATERLTDV 512
           +  + +          K+      DEV + I        P   GATKA+  GA +RLTD 
Sbjct: 71  KEAVKEL-------GQKRFKGKSPDEVLENIYGLMEGKDPATTGATKATTVGAVDRLTDT 123

Query: 513 KGYTGARKEAFDAETXXXXXXEGREYLVD 599
             YTG  KE FD E+       GRE + D
Sbjct: 124 SKYTGTHKELFD-ESGKGKGIAGREEMTD 151
>sp|Q9BW30|CG38_HUMAN Protein CGI-38
          Length = 176

 Score = 65.5 bits (158), Expect = 1e-10
 Identities = 41/129 (31%), Positives = 64/129 (49%), Gaps = 1/129 (0%)
 Frame = +3

Query: 219 KTIKKICTDAKLYT-KTFTANDVDIEFRKCIGNQKKDVDFAGFENFLDKFFIPAYAKSCK 395
           K   K+C D K+   K+ T  DVDI F K  G   + +++  F+  L++     +    K
Sbjct: 33  KNWAKLCKDCKVADGKSVTGTDVDIVFSKVKGKSARVINYEEFKKALEELATKRFKGKSK 92

Query: 396 KDAAAISDEVKKKIISNGPKAHGATKASKDGATERLTDVKGYTGARKEAFDAETXXXXXX 575
           ++A    D + + +    P   G TKA   GA +RLTD   YTG+ KE FD E+      
Sbjct: 93  EEAF---DAICQLVAGKEPANVGVTKAKTGGAVDRLTDTSRYTGSHKERFD-ESGKGKGI 148

Query: 576 EGREYLVDE 602
            GR+ ++D+
Sbjct: 149 AGRQDILDD 157
>sp|Q9CRB6|CG38_MOUSE Protein CGI-38 homolog
          Length = 176

 Score = 63.2 bits (152), Expect = 7e-10
 Identities = 41/129 (31%), Positives = 62/129 (48%), Gaps = 1/129 (0%)
 Frame = +3

Query: 219 KTIKKICTDAKLYT-KTFTANDVDIEFRKCIGNQKKDVDFAGFENFLDKFFIPAYAKSCK 395
           K   K+C D K+   K  T  DVDI F K      + +++  F+  L++     +    K
Sbjct: 33  KNWAKLCKDCKVADGKAVTGTDVDIVFSKVKAKSARVINYEEFKKALEELATKRFKGKSK 92

Query: 396 KDAAAISDEVKKKIISNGPKAHGATKASKDGATERLTDVKGYTGARKEAFDAETXXXXXX 575
           ++A    D + + I    P   G TKA   GA +RLTD   YTG+ KE FD E+      
Sbjct: 93  EEAF---DAICQLIAGKEPANIGVTKAKTGGAVDRLTDTSKYTGSHKERFD-ESGKGKGI 148

Query: 576 EGREYLVDE 602
            GR+ ++D+
Sbjct: 149 AGRQDILDD 157
>sp|Q7TQD2|P25A_MOUSE Tubulin polymerization-promoting protein (TPPP)
          Length = 218

 Score = 60.5 bits (145), Expect = 5e-09
 Identities = 45/141 (31%), Positives = 61/141 (43%), Gaps = 6/141 (4%)
 Frame = +3

Query: 198 GSTTATDKTI-----KKICTDAKLYT-KTFTANDVDIEFRKCIGNQKKDVDFAGFENFLD 359
           G T AT K +      K+C D  +   K  T  DVDI F K  G   + + F  F+  L+
Sbjct: 61  GDTRATGKEMHGKNWSKLCKDCHVIDGKNVTVTDVDIVFSKIKGKSCRTITFEQFQEALE 120

Query: 360 KFFIPAYAKSCKKDAAAISDEVKKKIISNGPKAHGATKASKDGATERLTDVKGYTGARKE 539
           +     +     ++A     EV + I    P   G TKA       RLTD   +TG+ KE
Sbjct: 121 ELAKKRFKDKSSEEAVR---EVHRLIEGRAPVISGVTKAVSSPTVSRLTDTSKFTGSHKE 177

Query: 540 AFDAETXXXXXXEGREYLVDE 602
            FD ++       GR  LVDE
Sbjct: 178 RFD-QSGKGKGKAGRVDLVDE 197
>sp|Q27957|P25A_BOVIN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha)
          Length = 218

 Score = 57.8 bits (138), Expect = 3e-08
 Identities = 43/129 (33%), Positives = 57/129 (44%), Gaps = 1/129 (0%)
 Frame = +3

Query: 219 KTIKKICTDAKLYT-KTFTANDVDIEFRKCIGNQKKDVDFAGFENFLDKFFIPAYAKSCK 395
           K   K+C D ++   ++ T  DVDI F K  G   + + F  F+  L++    A  +   
Sbjct: 73  KNWSKLCRDCQVIDGRSVTVTDVDIVFSKIKGKSCRTITFEQFKEALEEL---AKKRFKD 129

Query: 396 KDAAAISDEVKKKIISNGPKAHGATKASKDGATERLTDVKGYTGARKEAFDAETXXXXXX 575
           K A     EV K I    P   G TKA       RLTD   +TG+ KE FD  +      
Sbjct: 130 KSAEEAVREVHKLIEGKAPIISGVTKAISSPTVSRLTDTSKFTGSHKERFD-PSGRGKGR 188

Query: 576 EGREYLVDE 602
            GR  LVDE
Sbjct: 189 AGRVDLVDE 197
>sp|O94811|P25A_HUMAN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha) (p24) (p25)
          Length = 219

 Score = 56.2 bits (134), Expect = 9e-08
 Identities = 40/129 (31%), Positives = 56/129 (43%), Gaps = 1/129 (0%)
 Frame = +3

Query: 219 KTIKKICTDAKLYT-KTFTANDVDIEFRKCIGNQKKDVDFAGFENFLDKFFIPAYAKSCK 395
           K   K+C D ++   +  T  DVDI F K  G   + + F  F+  L++     +     
Sbjct: 74  KNWSKLCKDCQVIDGRNVTVTDVDIVFSKIKGKSCRTITFEQFQEALEELAKKRFKDKSS 133

Query: 396 KDAAAISDEVKKKIISNGPKAHGATKASKDGATERLTDVKGYTGARKEAFDAETXXXXXX 575
           ++A     EV + I    P   G TKA       RLTD   +TG+ KE FD  +      
Sbjct: 134 EEAVR---EVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERFD-PSGKGKGK 189

Query: 576 EGREYLVDE 602
            GR  LVDE
Sbjct: 190 AGRVDLVDE 198
>sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromosome I
          Length = 180

 Score = 53.1 bits (126), Expect = 8e-07
 Identities = 48/127 (37%), Positives = 57/127 (44%), Gaps = 5/127 (3%)
 Frame = +3

Query: 189 TKKGSTTATDKTIK---KICTDAK-LYTKTFTANDVDIEFRKCIGNQKKDVDFAGFENFL 356
           TK G+ TAT+ T K   K   DA  L  K  T     I F K  G +KK   F   +  L
Sbjct: 22  TKFGAATATEMTGKNFDKWLKDAGVLDNKAITGTMTGIAFSKVTGPKKK-ATFDETKKVL 80

Query: 357 DKFFIPAYAKSCKKDAAAISDEVKKKIIS-NGPKAHGATKASKDGATERLTDVKGYTGAR 533
             F     A+  KK      D + +K+     P   GA KA+  G   RLTD   YTGA 
Sbjct: 81  -AFVAEDRARQSKKPIQDELDAITEKLAKLEAPSVGGAAKANAAGVYSRLTDHTKYTGAH 139

Query: 534 KEAFDAE 554
           KE FDAE
Sbjct: 140 KERFDAE 146
>sp|Q9VV43|Y4893_DROME Hypothetical protein CG4893
          Length = 192

 Score = 52.0 bits (123), Expect = 2e-06
 Identities = 47/157 (29%), Positives = 72/157 (45%), Gaps = 4/157 (2%)
 Frame = +3

Query: 141 IKMSDMKTVFLEFCESTKKG---STTATDKTIKKICTDAKLYTKTFTANDVDIEFRKCIG 311
           +  SD    F +F +S   G   + + +DK +K+    AK+  K  T  D  I F+K   
Sbjct: 35  VSFSDQFKAFSKFGDSKSDGKLITLSQSDKWMKQ----AKVIDKKITTTDTGIHFKKF-- 88

Query: 312 NQKKDVDFAGFENFLDKFFIPAYAKSCKKDAAAISDEVKKKIISNGPKAHGATKASKDGA 491
            +   +  + +  FLD       AK+ K + +    E+K+K+ S G     +  A K  A
Sbjct: 89  -KAMKISLSDYNKFLDDL-----AKTKKVELS----EIKQKLASCGAPGVVSVSAGKAAA 138

Query: 492 T-ERLTDVKGYTGARKEAFDAETXXXXXXEGREYLVD 599
             +RLTD   YTG+ KE FDA +       GR  +VD
Sbjct: 139 AVDRLTDTSKYTGSHKERFDA-SGKGKGIAGRRNVVD 174
>sp|P32350|KNS1_YEAST Protein kinase KNS1
          Length = 737

 Score = 32.0 bits (71), Expect = 1.8
 Identities = 14/36 (38%), Positives = 23/36 (63%)
 Frame = +3

Query: 129 NHNNIKMSDMKTVFLEFCESTKKGSTTATDKTIKKI 236
           N   IK  D KT+ L++ E  K+G T  T+K++K++
Sbjct: 588 NSTVIKHFDRKTLSLQWPEKNKRGDTITTEKSMKRV 623
>sp|O46409|APOA4_PIG Apolipoprotein A-IV precursor (Apo-AIV) (ApoA-IV)
          Length = 382

 Score = 30.8 bits (68), Expect = 4.0
 Identities = 14/61 (22%), Positives = 31/61 (50%)
 Frame = +3

Query: 363 FFIPAYAKSCKKDAAAISDEVKKKIISNGPKAHGATKASKDGATERLTDVKGYTGARKEA 542
           F +   A+  K   +A +DE+++K++      HG  K + +G  + L +++ +   + E 
Sbjct: 243 FQMKKQAEELKAKISANADELRQKLVPVAENVHGHLKGNTEGLQKSLLELRSHLDQQVEE 302

Query: 543 F 545
           F
Sbjct: 303 F 303
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,096,354
Number of Sequences: 369166
Number of extensions: 1260005
Number of successful extensions: 3692
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3594
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3691
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00100

  1. Dr_sW_003_J11
  2. Dr_sW_005_N04
  3. Dr_sW_013_O15
  4. Dr_sW_028_P23
  5. Dr_sW_012_H16
  6. Dr_sW_016_N16