Planaria EST Database


DrC_00088

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00088
         (677 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q16795|NUEM_HUMAN  NADH-ubiquinone oxidoreductase 39 kDa ...   101   2e-21
sp|P34943|NUEM_BOVIN  NADH-ubiquinone oxidoreductase 39 kDa ...    99   8e-21
sp|Q9DC69|NUEM_MOUSE  NADH-ubiquinone oxidoreductase 39 kDa ...    94   3e-19
sp|P25284|NUEM_NEUCR  NADH-ubiquinone oxidoreductase 40 kDa ...    42   0.001
sp|P25811|TRME_BACSU  Probable tRNA modification GTPase trmE       34   0.31 
sp|Q01008|VP23_SHV21  Probable capsid protein VP23 (Gene 26 ...    34   0.40 
sp|P27169|PON1_HUMAN  Serum paraoxonase/arylesterase 1 (PON ...    31   2.6  
sp|O66841|NUOF_AQUAE  NADH-quinone oxidoreductase chain F (N...    31   3.4  
sp|Q8RFJ8|SYA_FUSNN  Alanyl-tRNA synthetase (Alanine--tRNA l...    30   4.4  
sp|Q9RCA7|TRME_BACHD  Probable tRNA modification GTPase trmE       30   5.8  
>sp|Q16795|NUEM_HUMAN NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial
           precursor (Complex I-39KD) (CI-39KD)
          Length = 377

 Score =  101 bits (251), Expect = 2e-21
 Identities = 77/208 (37%), Positives = 104/208 (50%), Gaps = 2/208 (0%)
 Frame = +2

Query: 8   STFLKTKGIGELEVLREFPEAVIFRPADMYGHNDHFFFYLMRWERRRFMGPKSYIPIWKY 187
           S +L+ K +GE  V   FPEA+I +P+D++G  D F        R    GP   IP+   
Sbjct: 178 SRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHR---FGP---IPLGSL 231

Query: 188 GKKTFKQPVAGYDVAKGIINVVNDPTISETIIEAVGPHRYRLDHLIWWLYMVLRFKPEKL 367
           G KT KQPV   DV+KGI+N V DP  +      VGP RY L HL+ +++ V      +L
Sbjct: 232 GWKTVKQPVYVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLLFHLVKYIFAV----AHRL 287

Query: 368 VISNMDPLLKLQ-VKIMETIKPIYPIVCLDKLERECFTD-TLNDLPTLEDIGVDNLTNLE 541
            +    PL   + V  +  I P  P +  DK+ER   TD  L  LP LED+G+   T LE
Sbjct: 288 FLPFPLPLFAYRWVARVFEISPFEPWITRDKVERMHITDMKLPHLPGLEDLGI-QATPLE 346

Query: 542 DRLFYEIRFRREDLYYWDSVGEIATIPP 625
            +    +  RR   Y W S  EI  + P
Sbjct: 347 LKAIEVL--RRHRTYRWLS-AEIEDVKP 371
>sp|P34943|NUEM_BOVIN NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial
           precursor (Complex I-39KD) (CI-39KD)
          Length = 380

 Score = 99.4 bits (246), Expect = 8e-21
 Identities = 79/211 (37%), Positives = 103/211 (48%), Gaps = 5/211 (2%)
 Frame = +2

Query: 8   STFLKTKGIGELEVLREFPEAVIFRPADMYGHNDHFFFYL--MRWERRRFMGPKSYIPIW 181
           S +L++K +GE EV   FPEA I +PA+++G  D F  Y   +RW    F G    +P+ 
Sbjct: 178 SKYLRSKAVGEKEVRETFPEATIIKPAEIFGREDRFLNYFANIRW----FGG----VPLI 229

Query: 182 KYGKKTFKQPVAGYDVAKGIINVVNDPTISETIIEAVGPHRYRLDHLIWWLYMVLR--FK 355
             GKKT KQPV   DV KGIIN + DP         VGP RY L  L+ +++ V    F 
Sbjct: 230 SLGKKTVKQPVYIVDVTKGIINAIKDPDARGKTFAFVGPSRYLLFDLVQYVFAVAHRPFL 289

Query: 356 PEKLVISNMDPLLKLQVKIMETIKPIYPIVCLDKLERECFTD-TLNDLPTLEDIGVDNLT 532
           P  L            +  +  I P  P    DK+ER   TD  L  LP LED+GV+  T
Sbjct: 290 PYPL-----PHFAYRWIGRLFEISPFEPWTTRDKVERIHTTDKILPHLPGLEDLGVE-AT 343

Query: 533 NLEDRLFYEIRFRREDLYYWDSVGEIATIPP 625
            LE +    +  RR   Y W S  EI  + P
Sbjct: 344 PLELKAIEVL--RRHRTYRWLS-SEIEDVQP 371
>sp|Q9DC69|NUEM_MOUSE NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial
           precursor (Complex I-39KD) (CI-39KD)
          Length = 377

 Score = 94.0 bits (232), Expect = 3e-19
 Identities = 70/198 (35%), Positives = 99/198 (50%), Gaps = 3/198 (1%)
 Frame = +2

Query: 8   STFLKTKGIGELEVLREFPEAVIFRPADMYGHNDHFFFYLMRWERRRFMGPKSYIPIWKY 187
           S  L++K +GE EV   FPEA+I RP+D++G  D F  +   +  R F+     +P+   
Sbjct: 178 SKSLRSKAVGEKEVRSVFPEAIIIRPSDIFGREDRFLNHFANY--RWFLA----VPLVSL 231

Query: 188 GKKTFKQPVAGYDVAKGIINVVNDPTISETIIEAVGPHRYRLDHLIWWLYMVLR--FKPE 361
           G KT KQPV   DV+KGI+N   DP          GP+RY L HL+ +++ +    F P 
Sbjct: 232 GFKTVKQPVYVADVSKGIVNATKDPDAVGKTFAFTGPNRYLLFHLVKYIFGMTHRTFIPY 291

Query: 362 KLVISNMDPLLKLQVKIMETIKPIYPIVCLDKLERECFTDTL-NDLPTLEDIGVDNLTNL 538
            L +     + KL       + P  P    DK+ER   +D +  DLP LED+GV   T L
Sbjct: 292 PLPLFVYSWIGKL-----FGLSPFEPWTTKDKVERIHISDVMPTDLPGLEDLGVQP-TPL 345

Query: 539 EDRLFYEIRFRREDLYYW 592
           E +    +  RR   Y W
Sbjct: 346 ELKSIEVL--RRHRTYRW 361
>sp|P25284|NUEM_NEUCR NADH-ubiquinone oxidoreductase 40 kDa subunit, mitochondrial
           precursor (Complex I-40KD) (CI-40KD)
          Length = 375

 Score = 42.0 bits (97), Expect = 0.001
 Identities = 44/199 (22%), Positives = 81/199 (40%), Gaps = 7/199 (3%)
 Frame = +2

Query: 14  FLKTKGIGELEVLREFPEAVIFRPADMYGHNDHFFFYLMRWERRRFMGPKSYIPIWKYGK 193
           F  TK  GE  V   FPE  I RPA M+G  D     L   +            +   G 
Sbjct: 177 FFATKARGEQVVRSIFPETTIVRPAPMFGFEDRLLHKLASVKN----------ILTSNGM 226

Query: 194 KTFKQPVAGYDVAKGIINVVNDPTISETIIEAVGPHRYRLDHLIWWLYMVLRFKPEKLVI 373
           +    PV   DV + +  ++ D   +    E  GP  Y    +   +   + +K  + V 
Sbjct: 227 QEKYNPVHVIDVGQALEQMLWDDNTASETFELYGPKTYTTAEISEMVDREI-YKRRRHV- 284

Query: 374 SNMDPLLKLQVKIMETIKPI------YPIVCLDKLERECFTDTLN-DLPTLEDIGVDNLT 532
                   +  KI++ I  +      +PI+  D++ERE     ++ +  T +D+G++   
Sbjct: 285 -------NVPKKILKPIAGVLNKALWWPIMSADEIEREFHDQVIDPEAKTFKDLGIEP-A 336

Query: 533 NLEDRLFYEIRFRREDLYY 589
           ++ +  ++ ++  R + YY
Sbjct: 337 DIANFTYHYLQSYRSNAYY 355
>sp|P25811|TRME_BACSU Probable tRNA modification GTPase trmE
          Length = 459

 Score = 34.3 bits (77), Expect = 0.31
 Identities = 43/174 (24%), Positives = 76/174 (43%), Gaps = 14/174 (8%)
 Frame = +2

Query: 197 TFKQPVAGYDVAKGI-INVVNDPTISET--IIEAVGPHRYRLDHLIWWLYMVLRFKPEKL 367
           T +  +  Y   +G+ + +V+   I ET  I+E +G  R R   ++    ++L      L
Sbjct: 255 TTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSR--QVLKEADLIL------L 306

Query: 368 VISNMDPLLKLQVKIMETIKPIYPIVCLDKLERECFTDT------LNDLPT-----LEDI 514
           V++  + L +  VK+ E ++ +  IV L+K + E   DT       N  P      L++ 
Sbjct: 307 VLNYSEELSEEDVKLFEAVEGMDVIVILNKTDLEPKIDTERVRELANGRPVVTTSLLKEE 366

Query: 515 GVDNLTNLEDRLFYEIRFRREDLYYWDSVGEIATIPPPPRAVI*DL*SININIP 676
           G+++L      LFY       DL Y  +   I  +    RA+   L  I  ++P
Sbjct: 367 GINDLEEAIQSLFYTGAIESGDLTYVSNTRHITILQQAKRAIEDALSGIEQDVP 420
>sp|Q01008|VP23_SHV21 Probable capsid protein VP23 (Gene 26 protein)
          Length = 304

 Score = 33.9 bits (76), Expect = 0.40
 Identities = 30/100 (30%), Positives = 46/100 (46%), Gaps = 19/100 (19%)
 Frame = +2

Query: 383 DPLLKLQVKIMETIKPI---YPIVCLDKL------ERECFTDTLN--------DLPTLED 511
           D + KLQ KI  +I P+   + +  LD L       R+ F D ++         L  LE+
Sbjct: 18  DEITKLQKKI-GSILPLQDPHKLQSLDTLGLNAVCSRDVFPDYVHMFSYLSKCTLAILEE 76

Query: 512 IGVDNL--TNLEDRLFYEIRFRREDLYYWDSVGEIATIPP 625
           +  DNL  T L+    Y+I+   E ++ WD    +  IPP
Sbjct: 77  VNPDNLILTRLDPSETYQIKNVYEPMFQWDGFSNLTVIPP 116
>sp|P27169|PON1_HUMAN Serum paraoxonase/arylesterase 1 (PON 1) (Serum
           aryldialkylphosphatase 1) (A-esterase 1) (Aromatic
           esterase 1) (K-45)
          Length = 355

 Score = 31.2 bits (69), Expect = 2.6
 Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
 Frame = +2

Query: 44  EVLREFPEAVIFRPADMYGHNDHFFF--YLMRWERRRFMGPKSYIPIWKYGKKTFKQPVA 217
           ++L    + V   P   YG NDH+F   YL  WE   ++G  ++  +  Y     +    
Sbjct: 162 KLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWE--MYLG-LAWSYVVYYSPSEVRVVAE 218

Query: 218 GYDVAKGI 241
           G+D A GI
Sbjct: 219 GFDFANGI 226
>sp|O66841|NUOF_AQUAE NADH-quinone oxidoreductase chain F (NADH dehydrogenase I, chain F)
           (NDH-1, chain F)
          Length = 426

 Score = 30.8 bits (68), Expect = 3.4
 Identities = 19/60 (31%), Positives = 27/60 (45%)
 Frame = +2

Query: 116 FFYLMRWERRRFMGPKSYIPIWKYGKKTFKQPVAGYDVAKGIINVVNDPTISETIIEAVG 295
           F   M WE  R++GP  Y      G K F  PV+G     G+  +  + T+ E I +  G
Sbjct: 229 FIISMGWEEYRYIGPSDYA-----GPKLF--PVSGKVKKPGVYELPMNTTLREVIFKYAG 281
>sp|Q8RFJ8|SYA_FUSNN Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
          Length = 867

 Score = 30.4 bits (67), Expect = 4.4
 Identities = 24/75 (32%), Positives = 40/75 (53%), Gaps = 9/75 (12%)
 Frame = +2

Query: 389 LLKLQVKIMETIKPIYP--------IVCLDKLERECFTDTLNDLPTLEDIGVDN-LTNLE 541
           + K+  K++E  +  YP        I  + K+E E F++TL+    L +  +DN LTN +
Sbjct: 322 MYKMVDKVVEKFEIAYPDLRKNVENIRKIVKIEEEKFSNTLDQGIQLVNQEIDNLLTNGK 381

Query: 542 DRLFYEIRFRREDLY 586
           ++L  EI F+  D Y
Sbjct: 382 NKLDGEISFKLYDTY 396
>sp|Q9RCA7|TRME_BACHD Probable tRNA modification GTPase trmE
          Length = 458

 Score = 30.0 bits (66), Expect = 5.8
 Identities = 41/174 (23%), Positives = 73/174 (41%), Gaps = 14/174 (8%)
 Frame = +2

Query: 197 TFKQPVAGYDVAKGI-INVVNDPTISET--IIEAVGPHRYRLDHLIWWLYMVLRFKPEKL 367
           T +  +  Y   +G+ + +V+   I ET  I+E +G  R R   ++    ++L      L
Sbjct: 254 TTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSR--EVLKEAELIL------L 305

Query: 368 VISNMDPLLKLQVKIMETIKPIYPIVCLDKLERECFTDTLN-----------DLPTLEDI 514
           V++  + L K    + E I  +  IV ++K + E   D                  ++D 
Sbjct: 306 VLNFGEELSKEDEALFEAISGMEAIVIVNKTDVEQKIDLTRVRELAAGRPVITTSIVQDQ 365

Query: 515 GVDNLTNLEDRLFYEIRFRREDLYYWDSVGEIATIPPPPRAVI*DL*SININIP 676
           G+D L      LF+E      D+ Y  +   IA +    RAV   L +I +++P
Sbjct: 366 GIDELEAAIASLFFESGIESGDVTYVSNSRHIALLEQAKRAVEDALEAIEMDVP 419
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,047,191
Number of Sequences: 369166
Number of extensions: 1741171
Number of successful extensions: 4748
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4742
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00088

  1. Dr_sW_022_M24
  2. Dr_sW_003_E11
  3. Dr_sW_022_B08