Planaria EST Database

DrC_01111

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01111
         (1343 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8N448|LNX2_HUMAN  Ligand of Numb-protein X 2 (Numb-bindi...    48   7e-05
sp|P70191|TRAF5_MOUSE  TNF receptor-associated factor 5            48   7e-05
sp|Q9Y4K3|TRAF6_HUMAN  TNF receptor-associated factor 6 (Int...    47   1e-04
sp|O00463|TRAF5_HUMAN  TNF receptor-associated factor 5 (RIN...    47   1e-04
sp|O70263|LNX1_MOUSE  Ubiquitin ligase LNX (Numb-binding pro...    45   6e-04
sp|P70196|TRAF6_MOUSE  TNF receptor-associated factor 6            44   8e-04
sp|Q8TBB1|LNX1_HUMAN  Ubiquitin ligase LNX (Numb-binding pro...    43   0.002
sp|Q91XL2|LNX2_MOUSE  Ligand of Numb-protein X 2 (Numb-bindi...    43   0.002
sp|Q60803|TRAF3_MOUSE  TNF receptor-associated factor 3 (CD4...    42   0.003
sp|P31111|ZIP1_YEAST  Synaptonemal complex protein ZIP1            41   0.009

>sp|Q8N448|LNX2_HUMAN Ligand of Numb-protein X 2 (Numb-binding protein 2) (PDZ
           domain-containing RING finger protein 1)
          Length = 690

 Score = 47.8 bits (112), Expect = 7e-05
 Identities = 40/155 (25%), Positives = 60/155 (38%), Gaps = 3/155 (1%)
 Frame = +2

Query: 80  HKFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXX 259
           H +    +V+DD  C IC   L+      CG  FCY+C+  ++                 
Sbjct: 36  HLYNYQNEVDDDLVCHICLQPLLQPLDTPCGHTFCYKCLRNFLQEKDFCPLDRKRLHFKL 95

Query: 260 XXXXIAQNHKKNKLISKLIVRCS-NEMCSHECQLIEMENHLNSCLNKAVKCPFNDVGCEH 436
                   H   KL+ KL+V C  + +C    Q  ++E HL +      +CP    G  H
Sbjct: 96  CKKSSILVH---KLLDKLLVLCPFSSVCKDVMQRCDLEAHLKN------RCP----GASH 142

Query: 437 GDV-INDKLNDHMLADIHNHN-KILIDLIGNLRNE 535
             V +  +      A+I N N   L+D  G L  E
Sbjct: 143 RRVALERRKTSRTQAEIENENGPTLLDPAGTLSPE 177

>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
          Length = 558

 Score = 47.8 bits (112), Expect = 7e-05
 Identities = 27/111 (24%), Positives = 47/111 (42%), Gaps = 1/111 (0%)
 Frame = +2

Query: 83  KFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXXX 262
           ++  +  + + YKC  C ++L N HQ  CG  FC +CI       ++             
Sbjct: 32  EYQFVEQLEERYKCAFCHSVLHNPHQTGCGHRFCQQCIRSLRELNSV--PICPVDKEVIK 89

Query: 263 XXXIAQNHKKNKLISKLIVRCSNEM-CSHECQLIEMENHLNSCLNKAVKCP 412
              + +++   + +  L V C N   C+    L   ++HL  C  +AV CP
Sbjct: 90  PQEVFKDNCCKREVLNLHVYCKNAPGCNARIILGRFQDHLQHCSFQAVPCP 140


 Score = 33.1 bits (74), Expect = 1.9
 Identities = 24/110 (21%), Positives = 48/110 (43%), Gaps = 4/110 (3%)
 Frame = +2

Query: 278 QNHKKNKLISKLIVRCSNEMCSHECQLIEMENHLNSCLNKAVKCPFNDVGC----EHGDV 445
           Q+H++N   +   V C N  C        +  HL  C      CPF   GC    + G++
Sbjct: 181 QDHEENSCPA-YPVSCPNR-CVQTIPRARVNEHLTVCPEAEQDCPFKHYGCTVKGKRGNL 238

Query: 446 INDKLNDHMLADIHNHNKILIDLIGNLRNEMRELREKVKGNRLEIEKLKE 595
           +     +H  A + +H  ++++    L   + +L + ++    +I++L E
Sbjct: 239 L-----EHERAALQDHMLLVLEKNYQLEQRISDLYQSLEQKESKIQQLAE 283

>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
           (RING finger protein 85)
          Length = 522

 Score = 47.4 bits (111), Expect = 1e-04
 Identities = 29/99 (29%), Positives = 43/99 (43%)
 Frame = +2

Query: 116 YKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXXXXXXIAQNHKKN 295
           Y+C IC   L  A Q  CG  FC  CI + + +                      N  K 
Sbjct: 68  YECPICLMALREAVQTPCGHRFCKACIIKSIRDAG--HKCPVDNEILLENQLFPDNFAKR 125

Query: 296 KLISKLIVRCSNEMCSHECQLIEMENHLNSCLNKAVKCP 412
           +++S L+V+C NE C H+ +L  +E+H   C    + CP
Sbjct: 126 EILS-LMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163

>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
          Length = 557

 Score = 47.0 bits (110), Expect = 1e-04
 Identities = 32/133 (24%), Positives = 57/133 (42%), Gaps = 1/133 (0%)
 Frame = +2

Query: 83  KFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXXX 262
           ++  +  + + YKC  C ++L N HQ  CG  FC  CI        +             
Sbjct: 32  EYQFVERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNTV--PICPVDKEVIK 89

Query: 263 XXXIAQNHKKNKLISKLIVRCSNEM-CSHECQLIEMENHLNSCLNKAVKCPFNDVGCEHG 439
              + +++   + +  L V CSN   C+ +  L   ++HL  CL + V+C  ++  C   
Sbjct: 90  SQEVFKDNCCKREVLNLYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQC--SNEKCRE- 146

Query: 440 DVINDKLNDHMLA 478
            V+   L +H+ A
Sbjct: 147 PVLRKDLKEHLSA 159


 Score = 46.2 bits (108), Expect = 2e-04
 Identities = 62/322 (19%), Positives = 119/322 (36%), Gaps = 32/322 (9%)
 Frame = +2

Query: 278  QNHKKNKLISKLIVRCSNEMCSHECQLIEMENHLNSCLNKAVKCPFNDVGCEHGDVINDK 457
            QNH++N L  +  V C N  C+      E++ HL  C      CPF   GC     + DK
Sbjct: 181  QNHEEN-LCPEYPVFCPNN-CAKIILKTEVDEHLAVCPEAEQDCPFKHYGC----AVTDK 234

Query: 458  ---LNDHMLADIHNHNKILIDLIGNLRNEMRELREKVKGNRLEIEKLKENDRIKNXXXXX 628
               L  H  + +  H +++++    L  ++ +L + ++    +I++L E  +        
Sbjct: 235  RRNLQQHEHSALREHMRLVLEKNVQLEEQISDLHKSLEQKESKIQQLAETIKKLEKEFKQ 294

Query: 629  XXXXXXXXXXXXXDLRDQMELGDRNLWYKFDEIDIEMKNKCKKDELLIEFDEKFDMKMNA 808
                         +++      D++ W +     +         +++ +   KFD++   
Sbjct: 295  FAQLFGKNGSFLPNIQVFASHIDKSAWLEAQVHQLL--------QMVNQQQNKFDLRPLM 346

Query: 809  RYCD---MKEEWNEKFDEKIEKMREENEKLDQIERVNKIDKYLNMRDF---------GEL 952
               D    K    E  D+++  + EE  K D    ++K     N   F         G+L
Sbjct: 347  EAVDTVKQKITLLENNDQRLAVLEEETNKHDTHINIHKAQLSKNEERFKLLEGTCYNGKL 406

Query: 953  IWQIGKINEIK---------WMISERFYDKQNPYKMFXXXXXXXXXXXXXXGIH------ 1087
            IW++      K          + S+ FY  +  Y++               G H      
Sbjct: 407  IWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRL--CARAYLNGDGSGRGSHLSLYFV 464

Query: 1088 --SGAFDDDLKWPCQADITIII 1147
               G FD  L+WP +  +T+++
Sbjct: 465  VMRGEFDSLLQWPFRQRVTLML 486

>sp|O70263|LNX1_MOUSE Ubiquitin ligase LNX (Numb-binding protein 1) (Ligand of
           Numb-binding protein 1) (Ligand of Numb-protein X 1)
          Length = 728

 Score = 44.7 bits (104), Expect = 6e-04
 Identities = 28/108 (25%), Positives = 47/108 (43%), Gaps = 6/108 (5%)
 Frame = +2

Query: 80  HKFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXX 259
           H +T   DV+DD  C IC   L++     CG  +C  C+  ++                 
Sbjct: 31  HFYTYTEDVDDDLICHICLQALLDPLDTPCGHTYCTLCLTNFLVE--------KDFCPVD 82

Query: 260 XXXXIAQNHKK-----NKLISKLIVRCS-NEMCSHECQLIEMENHLNS 385
               + Q+ KK     NKL++KL+V C   E C+   Q  ++++H  +
Sbjct: 83  RKPVVLQHCKKSSILVNKLLNKLLVTCPFTEHCTEVLQRCDLQHHFQT 130

>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
          Length = 530

 Score = 44.3 bits (103), Expect = 8e-04
 Identities = 34/147 (23%), Positives = 60/147 (40%)
 Frame = +2

Query: 116 YKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXXXXXXIAQNHKKN 295
           Y+C IC   L  A Q  CG  FC  CI + + +                      N  K 
Sbjct: 68  YECPICLMALREAVQTPCGHRFCKACIIKSIRDAG--HKCPVDNEILLENQLFPDNFAKR 125

Query: 296 KLISKLIVRCSNEMCSHECQLIEMENHLNSCLNKAVKCPFNDVGCEHGDVINDKLNDHML 475
           +++S L V+C N+ C  + +L  +E+H   C    V CP     C+       ++N H++
Sbjct: 126 EILS-LTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP----QCQR-PFQKCQVNTHII 179

Query: 476 ADIHNHNKILIDLIGNLRNEMRELREK 556
            D        ++   ++  E +E+ ++
Sbjct: 180 EDCPRRQVSCVNCAVSMAYEEKEIHDQ 206

>sp|Q8TBB1|LNX1_HUMAN Ubiquitin ligase LNX (Numb-binding protein 1) (Ligand of
           Numb-protein X 1)
          Length = 728

 Score = 43.1 bits (100), Expect = 0.002
 Identities = 28/114 (24%), Positives = 49/114 (42%), Gaps = 6/114 (5%)
 Frame = +2

Query: 62  YKLISQHKFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXX 241
           + L   H ++   +V+DD  C IC   L++     CG  +C  C+  ++           
Sbjct: 21  HSLEENHFYSYPEEVDDDLICHICLQALLDPLDTPCGHTYCTLCLTNFLVE--------K 72

Query: 242 XXXXXXXXXXIAQNHKK-----NKLISKLIVRCS-NEMCSHECQLIEMENHLNS 385
                     + Q+ KK     NKL++KL+V C   E C+   Q  ++E+H  +
Sbjct: 73  DFCPMDRKPLVLQHCKKSSILVNKLLNKLLVTCPFREHCTQVLQRCDLEHHFQT 126

>sp|Q91XL2|LNX2_MOUSE Ligand of Numb-protein X 2 (Numb-binding protein 2)
          Length = 687

 Score = 43.1 bits (100), Expect = 0.002
 Identities = 28/105 (26%), Positives = 43/105 (40%), Gaps = 2/105 (1%)
 Frame = +2

Query: 80  HKFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXX 259
           H +   G+V+DD  C IC   L+      CG  FC++C+  ++                 
Sbjct: 37  HLYNYQGEVDDDLVCHICLQPLLQPLDTPCGHTFCHKCLRNFLQEKDFCPLDRKRLHFKL 96

Query: 260 XXXXIAQNHKKNKLISKLIVRCS-NEMCSHECQLIEMENHL-NSC 388
                   H   KL+ KL+V C  + +C    Q  ++E HL N C
Sbjct: 97  CKKSSILVH---KLLDKLLVLCPFSPVCQDVMQRCDLEAHLKNRC 138

>sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD40 receptor associated factor
           1) (CRAF1) (TRAFAMN)
          Length = 567

 Score = 42.4 bits (98), Expect = 0.003
 Identities = 33/137 (24%), Positives = 56/137 (40%), Gaps = 6/137 (4%)
 Frame = +2

Query: 80  HKFTIIGDVNDDYKCVICKNILINAHQAICGCNFCYECINQYMANGAIXXXXXXXXXXXX 259
           +K   +  V D YKC  C+ +L N  Q  CG  FC  C+   +++ +             
Sbjct: 38  YKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHRFCESCMAALLSSSS---PKCTACQESI 94

Query: 260 XXXXIAQNHKKNKLISKLIVRCSNE--MCSHECQLIEMENHLNSCLNKAVKCPFNDVGCE 433
               + +++   + I  L V C NE   C+ +  L  +  HL +      +C F ++ C 
Sbjct: 95  IKDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKN------ECQFEELPCL 148

Query: 434 HGD----VINDKLNDHM 472
             D    V+   L DH+
Sbjct: 149 RADCKEKVLRKDLRDHV 165


 Score = 33.9 bits (76), Expect = 1.1
 Identities = 27/110 (24%), Positives = 45/110 (40%), Gaps = 19/110 (17%)
 Frame = +2

Query: 323 CSNEMCSHECQL-----IEMENHLNSCLNKAVKCPFNDVGCEHGDVINDKLNDHMLADIH 487
           C    C H+C +      E+  HL+ C+N    C F   GC      N ++  H  +   
Sbjct: 198 CVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGT-NQQIKAHEASSAV 256

Query: 488 NHNKILIDLIGNLRNEMRELR-EKVKGNR-------------LEIEKLKE 595
            H  +L +   +L  ++  L+ E V+ N+             +EIE+ KE
Sbjct: 257 QHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKE 306

>sp|P31111|ZIP1_YEAST Synaptonemal complex protein ZIP1
          Length = 875

 Score = 40.8 bits (94), Expect = 0.009
 Identities = 36/113 (31%), Positives = 55/113 (48%), Gaps = 4/113 (3%)
 Frame = +2

Query: 287 KKNKLISKLIVRCSNEMCSHECQLIEMENHLNSCLNK---AVKCPFNDVGCEHGDVINDK 457
           K N+ + KL V    ++ +HE +L E   HL +CL K    V+   +DV      VI  K
Sbjct: 635 KNNEQLQKLNVEVV-QLKAHELELEEQNRHLKNCLEKKETGVEESLSDVKTLKQQVIVLK 693

Query: 458 LNDHMLADIHNHNKILIDLIGNLRNEMRELREKVKGNRLEIE-KLKENDRIKN 613
                  DI      L D + +L    + L++KV+  + E+E K+KE + IKN
Sbjct: 694 SEKQ---DITAEKLELQDNLESLEEVTKNLQQKVQSQKRELEQKIKELEEIKN 743

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 125,616,709
Number of Sequences: 369166
Number of extensions: 2359781
Number of successful extensions: 8050
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7412
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8003
length of database: 68,354,980
effective HSP length: 114
effective length of database: 47,295,190
effective search space used: 15749298270
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01111

  1. Dr_sW_028_A21
  2. Dr_sW_002_C24
  3. Dr_sW_023_A21